FastQCFastQC Report
Tue 24 May 2016
ERR841183.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841183.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3795552
Sequences flagged as poor quality0
Sequence length24-50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCC344970.9088796570301236No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA175840.46327912250971665No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGA174000.458431342792827No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA163400.4305039161629191No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATC161360.42512920386810665No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG147140.3876642975777963No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAAT116180.3060951345153485No Hit
ATCCGAATTCAGTCTTCCTGAGGCTCTGGCTCACCTCCAGGCAGGTCCC111370.29342240601630537No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG106100.2795377325880399Illumina Single End Adapter 1 (100% over 32bp)
CTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAATCCT101040.26620633836659335No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTG95940.25276955762956216No Hit
CTGCGCGTCTGCTCCTCCTTCTCACGTTTCTCCTGCCGCCCTAATCCT92690.24420690323831684No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA91450.24093992125519556No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC87670.23098089553245482No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGT74920.19738894368987703No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG74350.19588718584279702No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGGTTGTGAGGGTTCCTGCTT70530.18582277360447177No Hit
AGGCCTGGTTTGCGCTGGAGAGTGGTCTTGGTTGTGAGGGTCATAAGAT70370.1854012275421335No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG69150.1821869388168045No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGT68360.1801055551340095No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG64940.1710950080515298No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAA60840.1602928902041126Illumina Single End Adapter 1 (100% over 32bp)
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCCG59140.15581396329176891No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTG58650.15452297847585805No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTA53950.1421400628946725No Hit
ATCCGAATTCAGTCTTCCTGAGGCTCTGGCTCACCTCCAGGCAGGTC51840.13658092419758708No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTG49960.13162775796511286No Hit
CTCTGCAGGTACCATGGAGCTGAGCTATAGGCTCTTCATCTGCCTCCT49340.12999426697355218No Hit
CTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAATC48530.1278601900329649No Hit
CTCGTATGCCGTCTTCTGCTTGAAAAAAA47390.12485667433880501Illumina Single End Adapter 1 (100% over 22bp)
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGAC47390.12485667433880501No Hit
CTCTTTCCCTTCGGTGTGCCACTGAAGATCCTGGTGTCGCCATGGGCCG46550.12264355751152928No Hit
ATTCCTGCTCACCGCAGTGTAGACAGGGAAACTTTCTTTGCAGTTCAGG46290.12195854516022966No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA46200.1217214255001644Illumina Single End Adapter 1 (100% over 32bp)
CTTCCGCCACGGCCGTCTCTGGAGAGCAGCAGCCATGGCCCTACGCTAC43910.11568804748294846No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAA43620.11492399524496043Illumina Single End Adapter 1 (100% over 32bp)
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT43400.11434436940924535No Hit
CTGCGCGTCTGCTCCTCCTTCTCACGTTTCTCCTGCCGCCCTAATC43400.11434436940924535No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTAG42440.11181509303521595No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCGCA2150.0214.992644
CTATCAT8000.0175.2016344
TAAGCCA8700.0113.11623444
CCCATAC3700.0112.838344
ACTTCTA8900.0103.8728244
TCGTGCG20000.086.3417343
AGGTCCC17950.074.6001343
TAATCGC2200.072.852343
ACATTGG5600.071.8912444
TTAAGCC5450.065.45711543
CAATGAA17800.064.1913543
GAACACA11450.063.81030344
CAGGCAC15800.061.34179744
CACATGC18750.060.43834344
ATAAGAT14400.059.24145543
TGTGTTA26500.058.5608142
TCCAGAA37900.057.97676543
CTAATCG2650.057.28775442
AATCCCG13600.055.50322343
TAATCCT40900.054.68715742
Produced by FastQC (version 0.11.5)