FastQCFastQC Report
Tue 24 May 2016
ERR841182.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841182.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17350636
Sequences flagged as poor quality0
Sequence length24-50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG3070931.7699235924262373Illumina Single End Adapter 1 (100% over 32bp)
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAA1981961.1422981843432136Illumina Single End Adapter 1 (100% over 32bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAA1643300.9471122557121249No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA1532640.8833336138225711No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA1530020.8818235827205413No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA1525760.8793683413103704Illumina Single End Adapter 1 (100% over 32bp)
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAA1466770.8453695876047427Illumina Single End Adapter 1 (100% over 32bp)
AAAAAAAAAAAAAAAAAAAAAAAAA1350630.778432560051401No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA1203610.6936979140130656No Hit
TCGGAAGAGCTCGTATGCCGTCTTCTGCTTG1175850.6776985005045348Illumina Single End Adapter 1 (100% over 31bp)
AAAAAAAAAAAAAAAAAAAAAAAA1169890.6742634679212911No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCC803680.4631991588089336No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA765220.4410328243875325No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAA745140.4294597615902956Illumina Single End Adapter 1 (100% over 32bp)
TCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAA704330.4059390099590585Illumina Single End Adapter 1 (100% over 31bp)
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA699680.40325899292682993Illumina Single End Adapter 1 (100% over 32bp)
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGA698950.4028382590701574Illumina Single End Adapter 1 (100% over 32bp)
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAA595280.34308828794517965Illumina Single End Adapter 1 (100% over 32bp)
AGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG573430.3304950896324492Illumina Single End Adapter 2 (100% over 34bp)
TCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA558340.3217980021020555Illumina Single End Adapter 1 (100% over 31bp)
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAA524370.3022194690730645Illumina Single End Adapter 1 (100% over 32bp)
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGGA503790.29035823240139436Illumina Single End Adapter 1 (100% over 32bp)
TCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAA491050.28301556208083667Illumina Single End Adapter 1 (100% over 31bp)
AAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG467740.2695808960547613Illumina Single End Adapter 2 (100% over 34bp)
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAA467440.2694079917301014Illumina Single End Adapter 1 (100% over 32bp)
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG461070.2657366565698226No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA442450.25500506148593055No Hit
AGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAA388600.22396873520947588Illumina Single End Adapter 2 (100% over 34bp)
ACTACTTCTCCCCCGGACTCCTTGGTAGTCTGTTAGTGGGAGATCCTTG388410.22385922913719128No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA380100.21906977934411162Illumina Single End Adapter 1 (100% over 32bp)
AAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAA365750.21079918914787907Illumina Single End Adapter 2 (100% over 34bp)
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGT364240.20992890404709086No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATC362110.20870128334200544No Hit
AAGCAGTGGTATCAACGCAGAGTGCAG345260.1989898237736069No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTAATAAAGGGAAGCAGTGGT340830.19643660324612883No Hit
AAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAA323830.18663869151540036Illumina Single End Adapter 2 (100% over 34bp)
AGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAA315040.18157259480286486Illumina Single End Adapter 2 (100% over 34bp)
CTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAATCCT311570.17957266811429853No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAAT311350.17944587160954792No Hit
TCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA294130.16952116337406883Illumina Single End Adapter 1 (100% over 31bp)
ATTCCTGCTCACCGCAGTGTAGACAGGGAAACTTTCTTTGCAGTTCAGG277810.16011516811256948No Hit
AGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA270220.15574068869867363Illumina Single End Adapter 2 (100% over 34bp)
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTT268210.1545822297234522Illumina Single End Adapter 1 (100% over 31bp)
AAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA261250.1505708493913422Illumina Single End Adapter 2 (100% over 34bp)
TCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAA259900.1497927799303726Illumina Single End Adapter 1 (100% over 31bp)
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA259620.14963140256069No Hit
TCGGAAGAGCTCGTATGCCGTCTTCTGCTTGA253220.14594277696794516Illumina Single End Adapter 1 (100% over 31bp)
ATCGGAAGAGCTCGTATGCCGTCTTCT244060.1406634315883291Illumina Single End Adapter 1 (100% over 27bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA242320.1396605865053016No Hit
AAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTCTTCTGCT224350.1293036174581727Illumina Single End Adapter 2 (100% over 32bp)
AAGCAGTGGTATCAACGCAGAGTGCAGA216910.12501559020660682No Hit
ATCCGAATTCAGTCTTCCTGAGGCTCTGGCTCACCTCCAGGCAGGTCCC203790.1174539077414799No Hit
TCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAA203750.11743085383152527Illumina Single End Adapter 1 (100% over 31bp)
TCGGAAGAGCTCGTATGCCGTCTTCTGCTTGGA201530.1161513618290419Illumina Single End Adapter 1 (100% over 31bp)
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG201080.115892005342052No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAA198920.11464709420450064No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCCG198810.11458369595212534No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAA189480.10920637145520198No Hit
TCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAA186870.10770210383066073Illumina Single End Adapter 1 (100% over 31bp)
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA186440.10745427429864819No Hit
TCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA183230.10560419802478711Illumina Single End Adapter 1 (100% over 31bp)
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA182760.1053333145828199No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTAATAAAGGGAAGCAGTG181410.10455524512185028No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAA180100.10380022957083533No Hit
CAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG174210.10040554133001232Illumina Single End Adapter 2 (100% over 34bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTAGTC17700.0231.9523244
ACATTGG12750.0129.4036444
CTATCAT18950.0107.3136344
ATCCTTG58250.099.3613743
TCGTGCG74450.095.7018643
TAAGCCA16350.095.04417444
TATTAGT16600.091.1323543
AATCGCA3600.085.2659644
AATCCCG36150.085.0032843
CAATGAA41750.084.439743
ACTTCTA16950.083.7568344
AGGTCCC42400.080.596743
CACATGC33650.076.39728544
AACGACA10050.071.2315543
TGTGTTA47450.070.8532742
ATGTTCT22100.070.31547544
TAATCCT79750.069.8882442
TAATCCC141800.069.58729642
GTTCAGG61600.069.5090243
CTATACG3950.067.9969144
Produced by FastQC (version 0.11.5)