##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841181.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11933255
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.13514200442377	38.0	35.0	39.0	31.0	39.0
2	36.86564621304078	38.0	35.0	39.0	33.0	39.0
3	37.17502014328865	39.0	37.0	39.0	33.0	40.0
4	37.75831598335911	39.0	37.0	40.0	33.0	41.0
5	38.16722788543444	40.0	37.0	41.0	33.0	41.0
6	38.20509048034254	40.0	37.0	41.0	33.0	41.0
7	38.147025518184265	40.0	37.0	41.0	33.0	41.0
8	38.04701047618609	40.0	37.0	41.0	33.0	41.0
9	38.00459195751704	39.0	37.0	41.0	33.0	41.0
10	37.8617642043181	39.0	37.0	41.0	33.0	41.0
11	37.774218350315984	39.0	36.0	40.0	33.0	41.0
12	37.67226410564427	39.0	36.0	40.0	33.0	41.0
13	37.51484259743046	39.0	36.0	40.0	32.0	41.0
14	37.457194872647904	39.0	36.0	40.0	32.0	41.0
15	37.42148617456009	39.0	36.0	40.0	32.0	41.0
16	37.38231480011112	39.0	36.0	40.0	32.0	41.0
17	37.328633637678905	39.0	36.0	40.0	32.0	41.0
18	37.19015616443292	38.0	35.0	40.0	31.0	41.0
19	37.11431248221881	38.0	35.0	40.0	31.0	41.0
20	37.001296377224826	38.0	35.0	40.0	31.0	41.0
21	36.82099494228524	38.0	35.0	40.0	31.0	41.0
22	36.87575208943411	38.0	35.0	40.0	31.0	41.0
23	36.82225662654489	38.0	35.0	40.0	31.0	41.0
24	36.76184486127213	38.0	35.0	40.0	31.0	41.0
25	36.73566998261613	38.0	35.0	40.0	31.0	41.0
26	36.71756956488425	38.0	35.0	40.0	31.0	41.0
27	36.721105780091335	38.0	35.0	40.0	31.0	41.0
28	36.72664025852437	38.0	35.0	40.0	31.0	41.0
29	36.70838892068916	38.0	35.0	40.0	31.0	41.0
30	36.703623215250346	38.0	35.0	40.0	31.0	41.0
31	36.631490382951476	38.0	35.0	40.0	31.0	41.0
32	36.558447166413096	38.0	35.0	40.0	31.0	41.0
33	36.48090075211535	38.0	35.0	40.0	31.0	41.0
34	36.41628770794981	38.0	35.0	40.0	31.0	41.0
35	36.30857865655214	37.0	34.0	40.0	31.0	41.0
36	36.16640512333185	37.0	34.0	40.0	31.0	41.0
37	36.110864524572015	37.0	34.0	40.0	31.0	41.0
38	36.02133946705516	37.0	34.0	40.0	31.0	41.0
39	35.875812658929334	36.0	34.0	40.0	30.0	41.0
40	35.728587501290285	36.0	34.0	39.0	30.0	41.0
41	35.53994581846189	36.0	34.0	39.0	30.0	41.0
42	35.393974131468504	35.0	34.0	39.0	30.0	41.0
43	35.26712947422451	35.0	33.0	39.0	30.0	40.0
44	35.14827673208856	35.0	33.0	39.0	30.0	40.0
45	35.12009421335486	35.0	33.0	39.0	30.0	40.0
46	35.00028626535842	35.0	33.0	38.0	30.0	40.0
47	34.97054204372219	35.0	33.0	38.0	30.0	40.0
48	34.973872246490416	35.0	33.0	38.0	30.0	40.0
49	35.04735349335147	35.0	33.0	39.0	30.0	40.0
50	35.673216539265034	36.0	34.0	39.0	30.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	3.0
20	9.0
21	28.0
22	152.0
23	441.0
24	1253.0
25	3233.0
26	7938.0
27	19696.0
28	45871.0
29	99385.0
30	193668.0
31	339403.0
32	537625.0
33	791099.0
34	1105309.0
35	1233421.0
36	1514230.0
37	1741489.0
38	1812259.0
39	1660340.0
40	826403.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	45.87481789335768	18.923512486743977	35.20166961989834
2	27.380366882296574	20.735893098739613	30.05497661786327	21.828763401100538
3	28.299763978897623	17.08944458154963	26.345854504910854	28.264936934641888
4	19.557823913089933	17.122218539702704	32.288566698692016	31.031390848515343
5	23.19026954506545	18.55533129896244	29.82275162979422	28.431647526177894
6	26.330887926219624	17.34209987132597	26.70138197834539	29.625630224109013
7	31.304878677276232	17.428404907127184	22.618371936240365	28.64834447935622
8	27.124091457024928	17.18075244348671	27.013367266516973	28.68178883297139
9	28.196221399777343	16.793180066964126	25.110969303848783	29.899629229409747
10	29.042243713052308	18.33112591660867	26.126316750961916	26.50031361937711
11	32.970392403413825	16.9029824637117	24.50050719606679	25.626117936807685
12	31.542383029609272	16.355797307608025	23.217412181336943	28.884407481445756
13	28.000591623995298	17.024751419457644	26.62847647184276	28.3461804847043
14	28.139329964875472	18.002866778594775	21.687628396443383	32.17017486008637
15	25.631137522830105	19.982385359233504	23.416393934429458	30.97008318350693
16	28.452974481815733	19.44482037801086	25.666157305781194	26.436047834392205
17	29.592881405785764	19.48722289098825	18.72250278737863	32.19739291584735
18	27.13866417838218	19.195098068381174	21.837026025170836	31.829211728065815
19	28.703761044241492	17.749222655511847	26.18504339344127	27.36197290680539
20	29.655999138541834	17.417200922966952	25.00900215406442	27.917797784426796
21	29.96019107946658	20.717180685403942	22.05311124249	27.269516992639474
22	30.276600977687984	19.607039319950843	24.288243232881555	25.828116469479617
23	29.522121164761835	21.067914831284508	22.920200733161238	26.489763270792423
24	32.41576585768091	20.851695534872924	20.369714717401077	26.36282389004509
25	29.768408526959206	21.20492560936577	23.31403051954687	25.71263534412816
26	30.73147801946419	20.987300271387394	22.564787490001944	25.71643421914647
27	29.886484089896744	21.007617793467077	23.21772760689247	25.888170509743713
28	29.97195804251597	20.07318867651604	24.125319174989237	25.82953410597876
29	32.791080971253045	19.602934557959266	24.39736256821886	23.20862190256883
30	28.937809260472765	21.164259399674897	22.95123309975908	26.94669824009326
31	28.08518761013979	20.491250314811893	27.446636389113355	23.976925685934958
32	27.935159761716072	19.14048924048272	25.730556592522973	27.193794405278236
33	26.729708777349515	19.276597238066653	26.776901496228415	27.216792488355416
34	26.226471568069186	18.22699797667377	30.298635021033153	25.24789543422389
35	29.053411850946137	18.436872406005957	24.55989272678939	27.949823016258517
36	28.122701125683374	19.207951608207004	24.337167832177535	28.33217943393208
37	25.10873962755733	20.63364160381228	27.45553654862036	26.802082220010032
38	30.269247422848185	20.83297783546433	22.418161254962193	26.479613486725295
39	27.993355247646072	21.075144024551477	24.697500725713386	26.23400000208907
40	28.460506528555307	20.07720222424063	22.500549690273992	28.961741556930072
41	27.187209441344883	20.91090492903836	23.092138964523496	28.809746665093268
42	26.865988083961177	21.58579336227393	25.914963348535508	25.633255205229382
43	25.23496509644233	23.413072117349536	24.59123897037556	26.76072381583257
44	23.786585580378457	23.222104390219386	25.017596059608472	27.973713969793685
45	26.23848860880541	20.086266471825397	27.279621981037717	26.395622938331474
46	25.052036426434693	21.392416755760518	24.443701735629364	29.111845082175424
47	28.094705249211565	20.42444231464426	24.23969006115591	27.241162374988264
48	24.540400459159333	22.45950982197933	24.705709557406514	28.294380161454818
49	27.648751778619427	21.010079468482935	22.15851165815477	29.18265709474287
50	24.35957696089844	26.147822709139596	23.283152925259035	26.209447404702928
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	5663.0
1	5431.5
2	5073.5
3	4840.0
4	4502.5
5	3649.0
6	2671.5
7	2442.5
8	2686.5
9	2886.0
10	2962.5
11	2927.0
12	2868.5
13	2978.0
14	3269.0
15	3613.5
16	3941.0
17	4545.0
18	5391.0
19	6534.5
20	7833.5
21	9671.5
22	11949.0
23	15388.5
24	19310.0
25	22912.0
26	27867.0
27	34243.0
28	41306.0
29	49081.0
30	57843.5
31	70477.5
32	87482.5
33	101911.5
34	118018.0
35	139214.5
36	158874.0
37	176555.0
38	196330.0
39	229370.0
40	270407.0
41	336807.0
42	443365.0
43	538921.0
44	576161.0
45	553700.0
46	561155.5
47	605003.5
48	682980.5
49	802333.5
50	901167.5
51	967314.5
52	1037356.0
53	1089896.5
54	1055768.0
55	1002590.0
56	986165.0
57	1006636.5
58	1027637.5
59	983841.5
60	929569.5
61	892607.0
62	837992.5
63	781341.0
64	738287.0
65	705396.0
66	639694.5
67	571416.0
68	503946.0
69	431515.0
70	377874.0
71	337650.5
72	312305.5
73	282536.0
74	237177.0
75	195540.0
76	167440.5
77	132695.5
78	105277.0
79	87901.5
80	69343.0
81	51395.0
82	37971.0
83	28560.0
84	21350.5
85	16100.0
86	11938.5
87	8263.0
88	5559.0
89	3898.5
90	2588.0
91	1574.0
92	1078.5
93	875.5
94	564.0
95	236.0
96	157.5
97	143.0
98	86.5
99	30.0
100	30.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	110799.0
25	102678.0
26	95152.0
27	93334.0
28	88342.0
29	78541.0
30	95775.0
31	90205.0
32	94092.0
33	89076.0
34	90022.0
35	96960.0
36	85877.0
37	91771.0
38	99614.0
39	106965.0
40	110945.0
41	111059.0
42	123849.0
43	129949.0
44	133081.0
45	201711.0
46	794686.0
47	1663086.0
48	2222787.0
49	3339382.0
50	1593517.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.175631624194637
#Duplication Level	Percentage of deduplicated	Percentage of total
1	55.34167866379804	9.505282861939472
2	12.883091549127352	4.425504692571728
3	7.006112514894982	3.6100322302048813
4	4.777953793825023	3.282574971206473
5	3.472481764202462	2.9821033801837498
6	2.6462332122071754	2.7270436106747833
7	2.0160932503559033	2.4239372491696742
8	1.6684055022791504	2.292473464554089
9	1.4079421648923753	2.1764066375126285
>10	7.910466152254878	23.945122838994454
>50	0.48171649155650775	5.712157414174141
>100	0.3189722710756602	10.90437077783907
>500	0.03440220908781615	4.053152667564612
>1k	0.02881407022018659	10.093803647114862
>5k	0.0033186222805847623	4.024512879294256
>10k+	0.002317767941995707	7.841520677001139
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCC	54145	0.4537320286878978	No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA	39889	0.33426755734290436	No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTG	35778	0.29981761053459427	No Hit
ATTCCTGCTCACCGCAGTGTAGACAGGGAAACTTTCTTTGCAGTTCAGG	35588	0.29822542131212315	No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA	35164	0.2946723253630296	No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG	34151	0.28618344282427555	No Hit
ATCCGAATTCAGTCTTCCTGAGGCTCTGGCTCACCTCCAGGCAGGTCCC	28830	0.24159376465180707	No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATC	25452	0.21328631626492522	No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGA	25186	0.2110572513534656	No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTAG	25162	0.21085613271483766	No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGT	24807	0.20788125285179943	No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA	24270	0.2033812233124994	No Hit
ACTACTTCTCCCCCGGACTCCTTGGTAGTCTGTTAGTGGGAGATCCTTG	22931	0.19216047926571586	No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAAT	22525	0.18875822229559328	No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCCG	21252	0.17809055450503655	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA	18341	0.15369653962812327	No Hit
CTGCGCGTCTGCTCCTCCTTCTCACGTTTCTCCTGCCGCCCTAATCCT	18002	0.15085573885750367	No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATC	17197	0.14410988452019166	No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGT	17015	0.14258473484392986	No Hit
CTCTTTCCCTTCGGTGTGCCACTGAAGATCCTGGTGTCGCCATGGGCCG	16348	0.13699531267872847	No Hit
AAAACAGCCGGGGCTCCAGCGGGAGAACGATAATGCAAAGTGCTATGTT	16339	0.136919893189243	No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG	15709	0.1316405289252597	No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC	15600	0.13072711510815782	No Hit
CTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAATCCT	15054	0.1261516660793723	No Hit
AAACAGCCGGGGCTCCAGCGGGAGAACGATAATGCAAAGTGCTATGTTC	15016	0.12583322823487808	No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGC	15004	0.12573266891556412	No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGAC	14691	0.1231097466701248	No Hit
ATTCCTGCTCACCGCAGTGTAGACAGGGAAACTTTCTTTGCAGTTCA	14086	0.11803988098804559	No Hit
AGCGAGCGGCCTTGAGAGGCTCTGGCTCTTGCTTCTTAGGCGGCCCGAG	13978	0.11713484711421988	No Hit
AGGCCTGGTTTGCGCTGGAGAGTGGTCTTGGTTGTGAGGGTCATAAGAT	13902	0.11649797142523143	No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTG	13860	0.11614601380763254	No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACC	13602	0.11398398844238222	No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTGC	13536	0.1134309121861554	No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG	13293	0.11139458597004756	No Hit
ATCCGAATTCAGTCTTCCTGAGGCTCTGGCTCACCTCCAGGCAGGTC	13253	0.111059388239001	No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGGTTGTGAGGGTTCCTGCTT	12970	0.10868786429184663	No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG	12855	0.10772417081508776	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAAC	12621	0.1057632640884654	No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCC	12167	0.10195876984108694	No Hit
CTTTCCGCGCCGATAGCGCTCACGCAAGCATGGTTAACGTCCCTAAAAC	12086	0.10127999443571767	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.12760139626614866	0.0	0.0	0.0	0.0
2	0.3549743971783055	0.0	0.0	0.0	0.0
3	0.47560368063868574	0.0	0.0	0.0	0.0
4	0.5691322275439518	0.0	0.0	0.0	0.0
5	0.7009319753914586	0.0	0.0	0.0	0.0
6	0.8028823652892694	0.0	0.0	0.0	0.0
7	0.896905328847829	0.0	0.0	0.0	0.0
8	0.9730371135117787	0.0	0.0	0.0	0.0
9	1.0316631966718217	0.0	0.0	0.0	0.0
10	1.0855127121644514	0.0	0.0	0.0	0.0
11	1.1371750624620023	0.0	0.0	0.0	0.0
12	1.1772144314355135	0.0	0.0	0.0	0.0
13	1.2089660365088988	0.0	0.0	0.0	0.0
14	1.2416897150023192	4.189971638081982E-5	0.0	0.0	0.0
15	1.2807905303289002	4.189971638081982E-5	0.0	0.0	0.0
16	1.3048661073613192	4.189971638081982E-5	0.0	0.0	0.0
17	1.3307601320846658	4.189971638081982E-5	0.0	0.0	0.0
18	1.3550452076989892	4.189971638081982E-5	0.0	0.0	0.0
19	1.422453471412452	4.189971638081982E-5	0.0	0.0	0.0
20	1.4409396262796696	4.189971638081982E-5	0.0	0.0	0.0
21	1.4778867961842765	4.189971638081982E-5	0.0	0.0	0.0
22	1.4912695655963104	4.189971638081982E-5	0.0	0.0	0.0
23	1.5050713321721525	4.189971638081982E-5	0.0	0.0	0.0
24	1.5187725394286806	4.189971638081982E-5	0.0	0.0	0.0
25	1.5351301887037527	4.189971638081982E-5	0.0	0.0	0.0
26	1.5885942268056787	4.189971638081982E-5	0.0	0.0	0.0
27	1.600828943988878	4.189971638081982E-5	0.0	0.0	0.0
28	1.614924008579386	4.189971638081982E-5	0.0	0.0	0.0
29	1.6279883401469255	4.189971638081982E-5	0.0	0.0	0.0
30	1.6401560177839156	4.189971638081982E-5	0.0	0.0	0.0
31	1.6519382180302022	4.189971638081982E-5	0.0	0.0	0.0
32	1.6640639959508114	4.189971638081982E-5	0.0	0.0	0.0
33	1.6758294363105457	4.189971638081982E-5	0.0	0.0	0.0
34	1.686941241094739	4.189971638081982E-5	0.0	0.0	0.0
35	1.6997793141938222	4.189971638081982E-5	0.0	0.0	0.0
36	1.7106900003393877	4.189971638081982E-5	0.0	0.0	0.0
37	1.7197235791910925	4.189971638081982E-5	0.0	0.0	0.0
38	1.7262599349465004	4.189971638081982E-5	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTAGTC	2610	0.0	189.61816	44
TAAGCCA	5960	0.0	187.7808	44
ATGTTCT	2580	0.0	112.528404	44
CTATACG	260	0.0	109.92711	44
AATCGCA	345	0.0	104.64457	44
CTATCAT	1830	0.0	91.24234	44
TTAAGCC	4545	0.0	82.480385	43
ACATTGG	1125	0.0	81.56463	44
ACTTCTA	1805	0.0	77.505104	44
TATTAGT	2280	0.0	75.21706	43
CGTGCGC	3125	0.0	66.909744	44
AATCCCG	3710	0.0	63.93379	43
ATCCTTG	4635	0.0	63.264748	43
AGGTCCC	6110	0.0	62.856297	43
TCGTGCG	7070	0.0	61.42754	43
ATTAAGC	6025	0.0	59.642315	42
CCTTAAC	2290	0.0	58.22325	43
TGTGTTA	8205	0.0	57.85428	42
TTCAGGA	3865	0.0	56.82349	44
CGCTACC	1260	0.0	56.1115	44
>>END_MODULE