FastQCFastQC Report
Tue 24 May 2016
ERR841179.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841179.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6808861
Sequences flagged as poor quality0
Sequence length24-50
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCC327760.4813727288602308No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG300020.4406317003680939No Hit
ATTCCTGCTCACCGCAGTGTAGACAGGGAAACTTTCTTTGCAGTTCAGG235040.3451972363659649No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGT206200.3028406660086026No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA181970.26725468474095737No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCCG181460.266505660785262No Hit
ACTACTTCTCCCCCGGACTCCTTGGTAGTCTGTTAGTGGGAGATCCTTG176930.25985256564937953No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATC154680.22717455973913994No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTAG144930.21285498411555176No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG138040.2027358173415495No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG134490.19752202314014047No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAAT120650.17719556912676No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG118380.17386167818670406Illumina Single End Adapter 1 (100% over 32bp)
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG111160.16325784885313416No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATC111120.1631991018762169No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCGC105850.15545918766736463No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA105570.15504795882894362No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGA98360.14445881623960308No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTG94930.1394212629689459No Hit
ATTCCTGCTCACCGCAGTGTAGACAGGGAAACTTTCTTTGCAGTTCA93020.13661609482114556No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCC91160.13388436039449184No Hit
ATCCGAATTCAGTCTTCCTGAGGCTCTGGCTCACCTCCAGGCAGGTCCC90870.13345844481184152No Hit
CTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAATCCT87470.12846495177387232No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGT78780.1157021710385922No Hit
CTCTTTCCCTTCGGTGTGCCACTGAAGATCCTGGTGTCGCCATGGGCCG77880.11438036405795331No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA76110.11178081032936346No Hit
ACTACTTCTCCCCCGGACTCCTTGGTAGTCTGTTAGTGGGAGATCCT75780.11129614776979586No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGAC75270.11054712381410048No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTC75040.11020932869682609No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGGTTGTGAGGGTTCCTGCTT74700.10970997939302918No Hit
ACTACTTCTCCCCCGGACTCCTTGGTAGTCTGTTAGTGGGAGATCCTTGT71700.10530395612423282No Hit
CTTTCCGCGCCGATAGCGCTCACGCAAGCATGGTTAACGTCCCTAAAAC71460.10495147426272912No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCG70830.10402620937628188No Hit
AGGCCTGGTTTGCGCTGGAGAGTGGTCTTGGTTGTGAGGGTCATAAGAT70030.10285126983793619No Hit
ATTCCTGCTCACCGCAGTGTAGACAGGGAAACTTTCTTTGCAGTTCAGGA69050.10141196890346271No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG2500.0143.8189244
CGTGCGC29900.0105.3223644
ATGTTCT16150.091.35503444
TAAGCCA14800.078.7452244
TCGTGCG52650.076.8201343
ATCCTTG35250.066.0543643
AATCCCG26050.063.38685643
TTCAGGA31100.062.19024744
CGCTACC7500.059.51127644
CCAGAAT15050.059.31356444
ATTAGTC5000.057.0316444
CACATGC12750.056.3995844
ACGCTAC14150.055.7249143
TGTGTTA43250.054.8915642
ACGACAC3200.054.24205444
CTAAAAC17700.053.98223543
TCCTTGT31550.053.0508644
GTTCAGG57800.052.72231343
TCGCAAA6700.052.6133243
AGGTCCC23400.051.73465343
Produced by FastQC (version 0.11.5)