##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841179.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6808861
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.96880653019646	38.0	35.0	39.0	30.0	39.0
2	36.78339314020363	38.0	35.0	39.0	32.0	39.0
3	37.07511726263761	39.0	37.0	39.0	33.0	39.0
4	37.61587613552399	39.0	37.0	40.0	33.0	41.0
5	38.05338719647823	39.0	37.0	40.0	33.0	41.0
6	38.07163782606224	39.0	37.0	41.0	33.0	41.0
7	38.01578839691396	39.0	37.0	41.0	33.0	41.0
8	37.90937250738413	39.0	37.0	40.0	33.0	41.0
9	37.83060221085436	39.0	37.0	40.0	33.0	41.0
10	37.68161268088745	39.0	36.0	40.0	33.0	41.0
11	37.550799318711306	39.0	36.0	40.0	32.0	41.0
12	37.45232146756998	39.0	36.0	40.0	32.0	41.0
13	37.281194461158776	39.0	36.0	40.0	32.0	41.0
14	37.22043760329371	38.0	36.0	40.0	32.0	41.0
15	37.197602800233405	38.0	36.0	40.0	32.0	41.0
16	37.12546048450688	38.0	35.0	40.0	31.0	41.0
17	37.09187880322421	38.0	35.0	40.0	31.0	41.0
18	36.9484634801621	38.0	35.0	40.0	31.0	41.0
19	36.86587360206061	38.0	35.0	40.0	31.0	41.0
20	36.740579812100734	38.0	35.0	40.0	31.0	41.0
21	36.54810356680802	38.0	35.0	40.0	30.0	41.0
22	36.5597648417261	38.0	35.0	40.0	31.0	41.0
23	36.54376613063477	38.0	35.0	40.0	31.0	41.0
24	36.49766032821055	38.0	35.0	40.0	31.0	41.0
25	36.459805111918705	38.0	35.0	40.0	31.0	41.0
26	36.38853314656737	38.0	35.0	40.0	30.0	41.0
27	36.35874714133367	38.0	34.0	40.0	31.0	41.0
28	36.344289535599074	38.0	34.0	40.0	31.0	41.0
29	36.34102651756378	38.0	34.0	40.0	31.0	41.0
30	36.32304535666568	38.0	34.0	40.0	31.0	41.0
31	36.22900893036168	37.0	34.0	40.0	31.0	41.0
32	36.146890949531155	37.0	34.0	40.0	31.0	41.0
33	36.0351034118628	37.0	34.0	40.0	31.0	41.0
34	35.98158962743212	37.0	34.0	40.0	31.0	41.0
35	35.86990345819269	37.0	34.0	40.0	30.0	41.0
36	35.738521718731164	36.0	34.0	39.0	30.0	41.0
37	35.65227694655853	36.0	34.0	39.0	30.0	41.0
38	35.59304428011976	36.0	34.0	39.0	30.0	41.0
39	35.42336890589526	36.0	34.0	39.0	30.0	41.0
40	35.29373211625	35.0	33.0	39.0	30.0	40.0
41	35.12563484376352	35.0	33.0	39.0	30.0	40.0
42	34.992959807401455	35.0	33.0	39.0	29.0	40.0
43	34.886953977184525	35.0	33.0	38.0	29.0	40.0
44	34.7871288421607	35.0	33.0	38.0	29.0	40.0
45	34.75355694566274	35.0	33.0	38.0	29.0	40.0
46	34.66693615433642	35.0	33.0	38.0	29.0	40.0
47	34.64563264275803	35.0	33.0	38.0	29.0	40.0
48	34.647453874699565	35.0	33.0	38.0	29.0	40.0
49	34.75895378341578	35.0	33.0	38.0	29.0	40.0
50	35.33679275543338	36.0	33.0	39.0	30.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	3.0
21	25.0
22	74.0
23	221.0
24	649.0
25	1880.0
26	5177.0
27	13110.0
28	30780.0
29	66350.0
30	128053.0
31	221870.0
32	346368.0
33	501176.0
34	677018.0
35	781123.0
36	932929.0
37	1024427.0
38	962237.0
39	776742.0
40	338649.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	47.75060028395352	19.459745176175574	32.78965453987091
2	28.18722544049585	20.383952617038297	28.576908825132424	22.851913117333428
3	28.19926857076389	15.88455102843192	27.648956264491225	28.26722413631296
4	20.34102326365599	17.06896351680553	31.177593433027933	31.412419786510547
5	23.92024157931848	18.083303507003595	27.508330688495477	30.48812422518245
6	26.792278473594923	17.807780185261528	27.193564386172664	28.206376954970885
7	31.228835483644033	18.89903465498855	22.39596314273415	27.476166718633266
8	28.03634851702803	15.839066181553713	27.435836918979543	28.688748382438707
9	27.590194600829715	16.98212079817755	25.587245796323348	29.840438804669382
10	27.406948093080473	18.23351952698109	27.164499319342838	27.1950330605956
11	31.695271793623046	16.909436100986643	25.13828671197723	26.257005393413085
12	31.328264742076538	16.316106320866293	23.07971920707443	29.27590972998274
13	27.43325205199519	16.565957213695505	27.37190552134931	28.628885212959993
14	28.46753664085667	18.60413951760801	21.695963539276246	31.23236030225907
15	26.314694924745858	18.809944864493488	23.438075766269865	31.437284444490786
16	27.572908302871802	20.813921741095903	25.36679776544124	26.246372190591057
17	28.88274558696381	19.308104542007833	18.951921620958338	32.85722825007002
18	27.419431825675396	19.090432305785065	22.466312647592602	31.023823220946937
19	28.03504139679162	17.417509330855776	26.283324039072024	28.264125233280573
20	28.943651515282802	17.274783550435235	25.220414985707595	28.561149948574364
21	30.261375581025963	20.703580231701014	21.240292612817328	27.794751574455702
22	30.116520222692166	19.204386754260366	24.633708927234675	26.045384095812796
23	29.506241939731183	21.473283123271276	22.79914364531748	26.221331291680062
24	32.75497913674548	20.808986995034854	20.498817643655816	25.93721622456384
25	29.697338791947818	22.24365133490534	22.6811691149053	25.377840758241536
26	31.553931367699388	21.11766435544781	21.92038272251121	25.40802155434159
27	30.03205331544873	21.47912002924374	23.09696656606578	25.391860089241746
28	30.81821627240319	20.72586275025842	23.8671443007211	24.58877667661729
29	31.929177924026362	19.901746968098877	24.34170852727232	23.827366580602437
30	29.076515304422983	22.417367012681144	22.538593843063882	25.96752383983199
31	28.228572750261655	20.92889326683892	26.888369931984972	23.954164050914454
32	28.96062368160528	19.522876508462716	25.503215073882135	26.01328473604987
33	26.797971182767956	18.8093719315095	27.083085465000988	27.30957142072155
34	26.33301901212063	18.28576378849263	30.405008266803257	24.976208932583486
35	29.70326599156184	19.13919592185811	24.937593034558567	26.219945052021487
36	28.287051720251423	19.711055819707795	23.01463707753844	28.987255382502347
37	26.006251964480537	20.0996063932914	27.806501104405758	26.087640537822303
38	30.429027494878635	19.97682149297933	22.78503935760518	26.80911165453686
39	28.127826745216705	20.18608974365453	25.763509673385332	25.922573837743435
40	28.22359066848248	19.584503999007573	23.356041180167324	28.835864152342626
41	26.359226832792952	21.417091619907154	23.50822706485208	28.71545448244781
42	26.222907981314986	20.901246504930768	26.87446632176403	26.00137919199022
43	24.92686613000725	23.771187630473207	24.73712865962945	26.56481757989009
44	23.67303335894893	22.249113829131087	25.40840062793989	28.66945218398009
45	26.72381054928569	19.322985294749433	27.315968655336864	26.637235500628016
46	24.99797191279391	21.10343466500896	24.10701795785755	29.791575464339576
47	28.04663023272646	20.557726806063144	24.215044921656528	27.180598039553868
48	24.229356472069146	22.27858729940824	25.14366678554662	28.348389442975996
49	28.73116759208141	21.27723066713844	22.19938706475333	27.792214676026827
50	25.041841177232886	24.372382341506725	24.098312290966444	26.487464190293952
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2754.0
1	2799.5
2	2711.0
3	2526.5
4	2361.5
5	1950.0
6	1516.5
7	1472.5
8	1662.0
9	1810.5
10	1876.0
11	1892.5
12	1867.5
13	1877.0
14	2039.5
15	2310.0
16	2616.5
17	3007.0
18	3421.5
19	4269.5
20	5350.5
21	6923.5
22	8648.5
23	10632.0
24	13136.5
25	15806.0
26	18902.0
27	22715.5
28	27402.0
29	32615.5
30	37787.0
31	44191.5
32	52603.0
33	61261.0
34	72185.0
35	86512.5
36	99843.0
37	110022.0
38	119454.0
39	136033.5
40	159563.0
41	197144.0
42	251916.5
43	290597.5
44	301111.5
45	300279.0
46	316892.5
47	348072.5
48	393780.5
49	448418.5
50	493851.5
51	532519.5
52	583173.5
53	623067.0
54	606146.0
55	576798.5
56	565877.0
57	574565.5
58	579495.5
59	550794.0
60	527793.0
61	520659.0
62	501261.0
63	470016.5
64	429311.0
65	393590.5
66	357275.0
67	323469.0
68	290032.5
69	252325.5
70	221850.5
71	199463.5
72	184151.5
73	167143.5
74	141470.5
75	116528.0
76	99916.0
77	80732.0
78	64725.0
79	53001.0
80	40695.0
81	29791.0
82	21892.5
83	16296.5
84	12151.5
85	9038.0
86	6569.5
87	4667.5
88	3309.5
89	2316.5
90	1468.0
91	829.5
92	568.0
93	483.5
94	321.0
95	141.5
96	89.0
97	83.0
98	47.5
99	12.0
100	12.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	66735.0
25	62822.0
26	59084.0
27	58153.0
28	53362.0
29	48089.0
30	61883.0
31	56266.0
32	63932.0
33	56662.0
34	57497.0
35	60152.0
36	53006.0
37	54283.0
38	60337.0
39	68142.0
40	68210.0
41	66454.0
42	71231.0
43	72217.0
44	73743.0
45	105098.0
46	396216.0
47	885070.0
48	1224853.0
49	1817321.0
50	1088043.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.340862256953532
#Duplication Level	Percentage of deduplicated	Percentage of total
1	55.5743667851342	10.748561730086939
2	13.199715017531977	5.105877399702538
3	7.042665540653995	4.086336724307463
4	4.857205726168483	3.757701876140423
5	3.473065889346146	3.3586044487583817
6	2.6328458862234987	3.055290577754126
7	2.083105659526756	2.820234173931113
8	1.6418713713679176	2.540416642980983
9	1.3120007268610767	2.28377028053187
>10	7.4433401549040825	24.96760309706935
>50	0.40055770542345853	5.36226992383235
>100	0.2813098895929516	10.813763806913999
>500	0.02967749729413253	3.9630938286157122
>1k	0.02430412892282912	9.074738416482086
>5k	0.0025626802605700797	3.4982106280265586
>10k+	0.00140534078805456	4.56352644486611
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCC	32776	0.4813727288602308	No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG	30002	0.4406317003680939	No Hit
ATTCCTGCTCACCGCAGTGTAGACAGGGAAACTTTCTTTGCAGTTCAGG	23504	0.3451972363659649	No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGT	20620	0.3028406660086026	No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA	18197	0.26725468474095737	No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCCG	18146	0.266505660785262	No Hit
ACTACTTCTCCCCCGGACTCCTTGGTAGTCTGTTAGTGGGAGATCCTTG	17693	0.25985256564937953	No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATC	15468	0.22717455973913994	No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTAG	14493	0.21285498411555176	No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG	13804	0.2027358173415495	No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG	13449	0.19752202314014047	No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAAT	12065	0.17719556912676	No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG	11838	0.17386167818670406	Illumina Single End Adapter 1 (100% over 32bp)
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG	11116	0.16325784885313416	No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATC	11112	0.1631991018762169	No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCGC	10585	0.15545918766736463	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA	10557	0.15504795882894362	No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGA	9836	0.14445881623960308	No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTG	9493	0.1394212629689459	No Hit
ATTCCTGCTCACCGCAGTGTAGACAGGGAAACTTTCTTTGCAGTTCA	9302	0.13661609482114556	No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCC	9116	0.13388436039449184	No Hit
ATCCGAATTCAGTCTTCCTGAGGCTCTGGCTCACCTCCAGGCAGGTCCC	9087	0.13345844481184152	No Hit
CTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAATCCT	8747	0.12846495177387232	No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGT	7878	0.1157021710385922	No Hit
CTCTTTCCCTTCGGTGTGCCACTGAAGATCCTGGTGTCGCCATGGGCCG	7788	0.11438036405795331	No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA	7611	0.11178081032936346	No Hit
ACTACTTCTCCCCCGGACTCCTTGGTAGTCTGTTAGTGGGAGATCCT	7578	0.11129614776979586	No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGAC	7527	0.11054712381410048	No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTC	7504	0.11020932869682609	No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGGTTGTGAGGGTTCCTGCTT	7470	0.10970997939302918	No Hit
ACTACTTCTCCCCCGGACTCCTTGGTAGTCTGTTAGTGGGAGATCCTTGT	7170	0.10530395612423282	No Hit
CTTTCCGCGCCGATAGCGCTCACGCAAGCATGGTTAACGTCCCTAAAAC	7146	0.10495147426272912	No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCG	7083	0.10402620937628188	No Hit
AGGCCTGGTTTGCGCTGGAGAGTGGTCTTGGTTGTGAGGGTCATAAGAT	7003	0.10285126983793619	No Hit
ATTCCTGCTCACCGCAGTGTAGACAGGGAAACTTTCTTTGCAGTTCAGGA	6905	0.10141196890346271	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.11120802730441993	0.0	0.0	0.0	0.0
2	0.24647294165646794	0.0	0.0	0.0	0.0
3	0.3296439742271138	0.0	0.0	0.0	0.0
4	0.39203326371327013	0.0	0.0	0.0	0.0
5	0.4846919330560574	0.0	0.0	0.0	0.0
6	0.5603433525812908	0.0	0.0	0.0	0.0
7	0.6356276035007911	0.0	0.0	0.0	0.0
8	0.6899685571492794	0.0	0.0	0.0	0.0
9	0.7353946570505698	0.0	0.0	0.0	0.0
10	0.7769581432195488	0.0	0.0	0.0	0.0
11	0.8192559665999938	0.0	0.0	0.0	0.0
12	0.8509499606468688	0.0	0.0	0.0	0.0
13	0.87688675095585	0.0	0.0	0.0	0.0
14	0.907655480116278	0.0	0.0	0.0	0.0
15	0.9439023648742425	0.0	0.0	0.0	0.0
16	0.967151480989258	0.0	0.0	0.0	0.0
17	0.9934848133924309	0.0	0.0	0.0	0.0
18	1.0179676160227091	0.0	0.0	0.0	0.0
19	1.0852475913372295	0.0	0.0	0.0	0.0
20	1.1055887320948394	0.0	0.0	0.0	0.0
21	1.1446848452332923	0.0	0.0	0.0	0.0
22	1.1586959992280648	0.0	0.0	0.0	0.0
23	1.1732358760150927	0.0	0.0	0.0	0.0
24	1.1845152955832114	0.0	0.0	0.0	0.0
25	1.2042395930831897	0.0	0.0	0.0	0.0
26	1.264939906982974	0.0	0.0	0.0	0.0
27	1.277276772135604	0.0	0.0	0.0	0.0
28	1.2907004563612035	0.0	0.0	0.0	0.0
29	1.3026995263965588	0.0	0.0	0.0	0.0
30	1.313817391778155	0.0	0.0	0.0	0.0
31	1.3246855825078527	0.0	0.0	0.0	0.0
32	1.336038435797118	0.0	0.0	0.0	2.9373488458642347E-5
33	1.3473619155979246	0.0	0.0	0.0	2.9373488458642347E-5
34	1.358083238885329	0.0	0.0	0.0	2.9373488458642347E-5
35	1.3690836103130906	0.0	0.0	0.0	2.9373488458642347E-5
36	1.3792762108082395	0.0	0.0	0.0	2.9373488458642347E-5
37	1.3875301610651178	0.0	0.0	0.0	2.9373488458642347E-5
38	1.3937132803856622	0.0	0.0	0.0	2.9373488458642347E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATACG	250	0.0	143.81892	44
CGTGCGC	2990	0.0	105.32236	44
ATGTTCT	1615	0.0	91.355034	44
TAAGCCA	1480	0.0	78.74522	44
TCGTGCG	5265	0.0	76.82013	43
ATCCTTG	3525	0.0	66.05436	43
AATCCCG	2605	0.0	63.386856	43
TTCAGGA	3110	0.0	62.190247	44
CGCTACC	750	0.0	59.511276	44
CCAGAAT	1505	0.0	59.313564	44
ATTAGTC	500	0.0	57.03164	44
CACATGC	1275	0.0	56.39958	44
ACGCTAC	1415	0.0	55.72491	43
TGTGTTA	4325	0.0	54.89156	42
ACGACAC	320	0.0	54.242054	44
CTAAAAC	1770	0.0	53.982235	43
TCCTTGT	3155	0.0	53.05086	44
GTTCAGG	5780	0.0	52.722313	43
TCGCAAA	670	0.0	52.61332	43
AGGTCCC	2340	0.0	51.734653	43
>>END_MODULE