##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841178.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4954425
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.08505406782825	38.0	35.0	39.0	31.0	39.0
2	36.847320728439726	38.0	35.0	39.0	32.0	39.0
3	37.198518899771415	39.0	37.0	39.0	33.0	40.0
4	37.727274305292745	39.0	37.0	40.0	33.0	41.0
5	38.061638434328906	39.0	37.0	41.0	33.0	41.0
6	38.118163863616864	40.0	37.0	41.0	33.0	41.0
7	38.092329584159614	40.0	37.0	41.0	33.0	41.0
8	37.94024412520121	39.0	37.0	41.0	33.0	41.0
9	37.851128233851554	39.0	37.0	40.0	33.0	41.0
10	37.711658567845916	39.0	36.0	40.0	33.0	41.0
11	37.58604600937546	39.0	36.0	40.0	32.0	41.0
12	37.535833522558114	39.0	36.0	40.0	32.0	41.0
13	37.37888978034787	39.0	36.0	40.0	32.0	41.0
14	37.36172754658714	39.0	36.0	40.0	32.0	41.0
15	37.348334872361576	39.0	36.0	40.0	32.0	41.0
16	37.30560176004279	39.0	36.0	40.0	32.0	41.0
17	37.25723247400052	39.0	36.0	40.0	32.0	41.0
18	37.099996265964265	38.0	35.0	40.0	31.0	41.0
19	37.0439003517058	38.0	35.0	40.0	31.0	41.0
20	36.940109296235185	38.0	35.0	40.0	31.0	41.0
21	36.7467140990125	38.0	35.0	40.0	31.0	41.0
22	36.814968840985586	38.0	35.0	40.0	31.0	41.0
23	36.78868708275935	38.0	35.0	40.0	31.0	41.0
24	36.72433531640907	38.0	35.0	40.0	31.0	41.0
25	36.709482517516705	38.0	35.0	40.0	31.0	41.0
26	36.69121395364894	38.0	35.0	40.0	31.0	41.0
27	36.68727861869042	38.0	35.0	40.0	31.0	41.0
28	36.732712644114784	38.0	35.0	40.0	31.0	41.0
29	36.741648477180036	38.0	35.0	40.0	31.0	41.0
30	36.71919911369044	38.0	35.0	40.0	31.0	41.0
31	36.666652729742204	38.0	35.0	40.0	31.0	41.0
32	36.58121495896142	38.0	35.0	40.0	31.0	41.0
33	36.50044364300303	38.0	35.0	40.0	31.0	41.0
34	36.412603571211136	38.0	34.0	40.0	31.0	41.0
35	36.28682273357371	37.0	34.0	40.0	31.0	41.0
36	36.12450375104922	37.0	34.0	40.0	31.0	41.0
37	36.11390749235663	37.0	34.0	40.0	31.0	41.0
38	36.04996273031721	37.0	34.0	40.0	31.0	41.0
39	35.884822294775475	37.0	34.0	40.0	30.0	41.0
40	35.707938476545486	36.0	34.0	39.0	30.0	41.0
41	35.5100375563704	36.0	34.0	39.0	30.0	41.0
42	35.39949970854384	36.0	34.0	39.0	30.0	41.0
43	35.233590498186814	35.0	33.0	39.0	30.0	40.0
44	35.121849635576794	35.0	33.0	39.0	30.0	40.0
45	35.08206257645359	35.0	33.0	39.0	30.0	40.0
46	34.97869952060555	35.0	33.0	38.0	30.0	40.0
47	34.89437149172145	35.0	33.0	38.0	29.0	40.0
48	34.89845113570752	35.0	33.0	38.0	29.0	40.0
49	35.111309684930745	35.0	33.0	39.0	30.0	40.0
50	36.04740555637219	37.0	34.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	7.0
21	20.0
22	75.0
23	238.0
24	616.0
25	1450.0
26	3683.0
27	8756.0
28	19586.0
29	42139.0
30	82876.0
31	144515.0
32	229240.0
33	337435.0
34	466125.0
35	516432.0
36	627261.0
37	710762.0
38	744737.0
39	683194.0
40	335278.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	49.53733278836596	15.246027541036547	35.216639670597495
2	28.910075336693964	19.497055662362435	28.80580491177079	22.78706408917281
3	31.06142488785278	15.931697422001545	23.75173708351625	29.255140606629425
4	22.532503771880695	18.05101500174087	29.41719775756016	29.999283468818277
5	25.700459690075032	18.345115729877836	26.337143058982626	29.617281521064502
6	27.772445843866844	19.260539820463528	24.388359900492993	28.578654435176638
7	33.48761965313835	20.77587207395409	20.180565857793788	25.555942415113762
8	29.455850073419214	17.23695484339757	27.974144325527178	25.333050757656032
9	28.55162809004072	19.7920848534391	24.027066713089816	27.629220343430365
10	30.02110234790112	18.811829828890335	26.590573073565547	24.576494749642997
11	33.16604853237258	17.12739621651352	23.954646603793577	25.751908647320327
12	32.14631364890981	16.44081805658578	21.69886515589599	29.714003138608412
13	27.91833562926071	17.427612689666308	26.849331658063246	27.804720023009732
14	26.50281314178739	18.873875374034323	21.406500249776713	33.216811234401575
15	25.302068353037942	19.61876504337032	25.107030583771074	29.972136019820667
16	27.85505886152278	21.87398133991331	26.382698294958544	23.888261503605364
17	29.375053613688774	20.75231737285356	18.560478763933254	31.312150249524418
18	26.17155371208566	21.624244993112217	22.09646931783204	30.107731976970083
19	28.43538856678626	17.27443648859353	26.439253798372164	27.850921146248055
20	28.069614536500197	17.05644953753463	25.12317776533099	29.750758160634184
21	29.841989736447722	20.52607517522215	22.36842822325497	27.26350686507516
22	29.677349036467398	18.775841797988665	24.444733748114057	27.102075417429873
23	28.822880556270402	20.894029075018796	22.607345958410914	27.675744410299885
24	32.66821074090333	20.357720623483047	21.03751696715562	25.936551668457998
25	28.668771163196393	21.06021566574071	22.908939207862183	27.36207396320071
26	30.574608672494797	20.599353294515662	23.207319739476652	25.618718293512888
27	27.744579284623626	20.95115737149138	24.029340254437155	27.27492308944784
28	28.329367409182566	19.59975647187269	26.210685224450575	25.860190894494174
29	30.959511621479326	18.862916251995415	26.25660890519433	23.920963221330936
30	27.418720750282365	21.83275731393699	24.10045546933485	26.648066466445798
31	28.23221276905286	19.67984040230497	27.724617180772075	24.363329647870096
32	27.036970978344616	18.89541244374124	25.88172875614008	28.185887821774063
33	25.146250146885475	18.293296136102942	28.617547584920437	27.94290613209115
34	24.67621746132765	19.48380506953074	29.471208112160223	26.368769356981392
35	29.69575109934527	18.84162730288877	24.688753708418247	26.77386788934772
36	26.678861642476566	18.975796203375932	24.710054848362574	29.635287305784924
37	24.4797365507268	20.64790884662353	26.384402376249756	28.487952226399916
38	29.969164193569714	21.611099890701343	21.06830972844362	27.351426187285327
39	27.162460164452344	20.884662516408273	26.210171913258712	25.742705405880674
40	26.68317975694868	19.6036161577453	23.457065384637716	30.256138700668306
41	25.263504510689543	23.405334983598028	22.51605353269383	28.815106973018594
42	26.75646037373456	21.000563886215325	26.098585194978348	26.144390545071776
43	24.537305692489507	23.90339039253828	24.92886075022026	26.63044316475195
44	23.870666710639302	23.036261544343347	22.909449352686178	30.183622392331174
45	26.516085735074572	19.11954218057023	28.312261717596073	26.052110366759123
46	24.295332726710196	20.935745331801826	24.848996702595276	29.919925238892702
47	29.471405233376487	19.844632369151455	23.12691135820195	27.557051039270107
48	22.7718542398322	23.27495617454818	25.265220893138896	28.687968692480727
49	28.078700457553214	22.051803851100075	20.820410634715124	29.049085056631586
50	22.632925896192102	28.897130602260688	24.092312215199176	24.377631286348027
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2342.0
1	2289.0
2	1893.5
3	1524.5
4	1431.0
5	1174.5
6	859.5
7	797.0
8	916.5
9	997.5
10	1021.0
11	1046.5
12	1098.5
13	1167.5
14	1230.5
15	1350.5
16	1521.0
17	1749.5
18	2049.0
19	2659.5
20	3486.0
21	4496.0
22	5456.0
23	6544.0
24	8011.5
25	9603.5
26	11538.0
27	14535.0
28	17932.5
29	20653.0
30	23722.0
31	30112.0
32	39302.0
33	45685.0
34	55359.0
35	69934.5
36	78732.0
37	82752.5
38	86645.0
39	96882.5
40	114540.5
41	146501.5
42	197934.0
43	238729.0
44	248882.5
45	236807.0
46	231345.5
47	235937.0
48	259205.5
49	304207.5
50	361114.0
51	401134.0
52	418591.5
53	458120.5
54	463824.0
55	420189.5
56	388693.5
57	396649.0
58	411711.5
59	392757.5
60	373545.5
61	367626.5
62	369355.5
63	363146.5
64	337690.0
65	314714.0
66	269437.5
67	225700.5
68	202348.0
69	178608.0
70	159771.0
71	146693.5
72	135439.5
73	116954.0
74	93829.0
75	76223.5
76	65030.0
77	51285.5
78	40259.5
79	31766.0
80	24052.0
81	18252.0
82	14056.0
83	10887.5
84	8459.0
85	6295.5
86	4481.0
87	2955.0
88	1814.5
89	1177.5
90	784.0
91	504.5
92	385.5
93	341.5
94	218.0
95	85.0
96	54.5
97	50.0
98	31.5
99	12.5
100	12.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	45254.0
25	42659.0
26	39151.0
27	36128.0
28	33448.0
29	31671.0
30	38327.0
31	39844.0
32	44016.0
33	37745.0
34	42353.0
35	45482.0
36	37568.0
37	39078.0
38	45667.0
39	50359.0
40	51004.0
41	49922.0
42	55141.0
43	56153.0
44	58327.0
45	88691.0
46	408377.0
47	661307.0
48	1036967.0
49	1309152.0
50	530634.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.029548621664972
#Duplication Level	Percentage of deduplicated	Percentage of total
1	56.98709061343688	10.274515210221876
2	12.557588621864985	4.528153092575631
3	7.032813101033508	3.803953372564982
4	4.610070756846921	3.3246997943954955
5	3.3356470491510617	3.007010532869118
6	2.549185638832207	2.757639985258522
7	1.9451398654875072	2.454899564652211
8	1.5833433970675306	2.2837573409776986
9	1.2624628192502991	2.0485471304445757
>10	7.366649926903696	23.230986442894245
>50	0.4150105507099771	5.1930351060075814
>100	0.28904827658966503	10.323298755771777
>500	0.03155708785714563	3.968007397842549
>1k	0.028352071121161043	10.613816380288208
>5k	0.00394463598207458	4.861519151106292
>10k+	0.002095587865477121	7.326160742129156
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG	43539	0.8787901724216232	No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCC	41408	0.8357781175413896	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC	26023	0.525247632167204	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA	21468	0.43330961716041716	No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGT	21375	0.4314325073040767	No Hit
CTCTGCAGGTACCATGGAGCTGAGCTATAGGCTCTTCATCTGCCTCCT	20054	0.404769473753261	No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG	19307	0.3896920429716869	No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATC	19215	0.3878351170922963	No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA	18064	0.3646033596229633	No Hit
CTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAATCCT	17639	0.35602516941925655	No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA	13324	0.26893130888044525	No Hit
ACTACTTCTCCCCCGGACTCCTTGGTAGTCTGTTAGTGGGAGATCCTTG	12515	0.2526024715279775	No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGA	12336	0.24898953965394574	No Hit
ATCCGAATTCAGTCTTCCTGAGGCTCTGGCTCACCTCCAGGCAGGTCCC	11562	0.2333671414947244	No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA	10706	0.21608965722561144	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA	10591	0.21376849987637314	No Hit
AGGCCTGGTTTGCGCTGGAGAGTGGTCTTGGTTGTGAGGGTCATAAGAT	10510	0.21213359774343138	No Hit
ACCAGCGAGCGGCCTTGAGAGGCTCTGGCTCTTGCTTCTTAGGCGGCCC	9844	0.19869106909479908	No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG	9280	0.18730730609505644	Illumina Single End Adapter 1 (100% over 32bp)
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTG	8988	0.18141358482568615	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG	8889	0.1794153711076462	No Hit
CGGCTCCTGGCCAGGTAACTTACTACTTCCAGTCAATATGCCGACCTT	8860	0.17883003577609916	No Hit
CTCTGCAGGTACCATGGAGCTGAGCTATAGGCTCTTCATCTGCCTC	8295	0.1674260887994066	No Hit
AGCGAGCGGCCTTGAGAGGCTCTGGCTCTTGCTTCTTAGGCGGCCCGAG	8137	0.16423702044132266	No Hit
CTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAATC	8124	0.163974628740974	No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTC	7963	0.16072500845204035	No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGT	7834	0.15812127542550344	No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAAT	7450	0.1503706282767425	No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCCG	7400	0.1493614294292476	No Hit
TCGGAAGAGCTCGTATGCCGTCTTCTGCTTG	7147	0.1442548832609233	Illumina Single End Adapter 1 (100% over 31bp)
CGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGT	6935	0.1399758801475449	No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG	6555	0.1323059689065835	No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTAG	6546	0.13212431311403441	No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTG	6496	0.13111511426653952	No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTG	6404	0.12925818838714886	No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGC	6268	0.12651316752196268	No Hit
CTGCGCGTCTGCTCCCCCTTCTCACGTTTCTCCTGCTGCCCTAATCCT	6143	0.1239901704032254	No Hit
CGGCACAGCTGGTTTGAGCAACTGAACTGGAAACAAGATGCAGGACCC	6076	0.1226378439475822	No Hit
ATTCCTGCTCACCGCAGTGTAGACAGGGAAACTTTCTTTGCAGTTCAGG	5694	0.11492756475272106	No Hit
CTCTGCAGGTACCATGGAGCTGAGCTATAGGCTCTTCATCTGCCTCCTG	5628	0.11359542227402776	No Hit
CAGGTACCATGGAGCTGAGCTATAGGCTCTTCATCTGCCTCCTGCTCT	5512	0.11125408094783955	No Hit
ACCACGCTTTTCATCTGTCCCGCTGCGTGTTTTCCTCTTGATCGGGAAC	5508	0.11117334504003996	No Hit
ACTACTTCTCCCCCGGACTCCTTGGTAGTCTGTTAGTGGGAGATCCT	5467	0.11034580198509412	No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAA	5338	0.10774206895855724	Illumina Single End Adapter 1 (100% over 32bp)
ATCCGAATTCAGTCTTCCTGAGGCTCTGGCTCACCTCCAGGCAGGTC	5322	0.10741912532735887	No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGC	5246	0.1058851430791666	No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG	5159	0.10412913708452544	No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGGTTGTGAGGGTTCCTGCTT	5124	0.103422697891279	No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATG	5109	0.10311993823703053	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.31955272307079025	0.0	0.0	0.0	0.0
2	0.5402846949948783	0.0	0.0	0.0	0.0
3	0.6811284861512689	0.0	0.0	0.0	0.0
4	0.7804740206986683	0.0	0.0	0.0	0.0
5	0.8898509917901674	0.0	0.0	0.0	0.0
6	1.0174540940674246	0.0	0.0	0.0	0.0
7	1.140031386084157	0.0	0.0	0.0	0.0
8	1.234552950140531	0.0	0.0	0.0	0.0
9	1.321465154886793	0.0	0.0	0.0	0.0
10	1.392351281934836	0.0	0.0	0.0	0.0
11	1.452862844830631	0.0	0.0	0.0	0.0
12	1.5102257073222423	0.0	0.0	0.0	0.0
13	1.5497459341901432	0.0	0.0	0.0	0.0
14	1.589669840597042	0.0	0.0	0.0	0.0
15	1.6239624174349192	0.0	0.0	0.0	0.0
16	1.6554897894306604	0.0	0.0	0.0	0.0
17	1.6873602890345498	0.0	0.0	0.0	0.0
18	1.7206840349788322	4.0367953899796644E-5	0.0	0.0	0.0
19	1.745308486857708	1.2110386169938994E-4	0.0	0.0	0.0
20	1.763756641789915	1.2110386169938994E-4	0.0	0.0	0.0
21	1.7839809866937133	1.2110386169938994E-4	0.0	0.0	0.0
22	1.7980088506738925	1.2110386169938994E-4	0.0	0.0	0.0
23	1.8131064654324165	1.2110386169938994E-4	0.0	0.0	0.0
24	1.8262462344268002	1.2110386169938994E-4	0.0	0.0	0.0
25	1.8397089470523824	1.2110386169938994E-4	0.0	0.0	0.0
26	1.8571680871140446	1.2110386169938994E-4	0.0	0.0	0.0
27	1.8702069362236788	1.2110386169938994E-4	0.0	0.0	0.0
28	1.8824586102322671	1.2110386169938994E-4	0.0	0.0	0.0
29	1.8946497323100058	1.2110386169938994E-4	0.0	0.0	0.0
30	1.9060334953097484	1.2110386169938994E-4	0.0	0.0	0.0
31	1.9171548666091422	1.2110386169938994E-4	0.0	0.0	0.0
32	1.9280542141620873	1.2110386169938994E-4	0.0	0.0	0.0
33	1.9393572412540305	1.2110386169938994E-4	0.0	0.0	0.0
34	1.9486822386048834	1.2110386169938994E-4	0.0	0.0	0.0
35	1.9598237938812273	1.2110386169938994E-4	0.0	0.0	0.0
36	1.9687451116930825	1.2110386169938994E-4	0.0	0.0	0.0
37	1.9763544710031942	1.2110386169938994E-4	0.0	0.0	0.0
38	1.981380281263719	1.2110386169938994E-4	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAAGCCA	1630	0.0	261.3408	44
CTATCAT	1095	0.0	163.5294	44
ACTTCTA	1150	0.0	145.87422	44
ACATTGG	785	0.0	144.06813	44
CCCATAC	495	0.0	106.620125	44
GAACACA	2150	0.0	97.313	44
TCGTGCG	5490	0.0	95.0703	43
TTAAGCC	1475	0.0	84.19931	43
CGTGCGC	1680	0.0	83.02498	44
CAATGAA	2350	0.0	82.46188	43
AATGAAG	1345	0.0	75.67594	44
AATCGCA	125	1.8261908E-6	75.39566	44
ATCCTTG	2020	0.0	71.55252	43
CCAGAAT	1350	0.0	69.8108	44
AGGTCCC	2130	0.0	65.59541	43
TGGTACA	640	0.0	64.79315	44
ATAAGAT	2075	0.0	64.754234	43
TCCAGAA	4385	0.0	64.33593	43
GTAGAGA	560	0.0	63.95168	44
AATCCCG	1515	0.0	59.008743	43
>>END_MODULE