##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841177.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	343203
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.7098451936609	37.0	35.0	39.0	30.0	39.0
2	36.51195356683945	38.0	35.0	39.0	32.0	39.0
3	36.64542850732657	38.0	35.0	39.0	32.0	39.0
4	37.04097283531904	38.0	35.0	39.0	33.0	40.0
5	37.65791091569712	39.0	36.0	40.0	33.0	41.0
6	37.67370331844418	39.0	36.0	40.0	33.0	41.0
7	37.602678298266625	39.0	36.0	40.0	33.0	41.0
8	37.51461671372336	39.0	36.0	40.0	33.0	41.0
9	37.48232969991521	39.0	36.0	40.0	33.0	41.0
10	37.295603476659586	39.0	35.0	40.0	32.0	41.0
11	37.182428475275564	38.0	35.0	40.0	32.0	41.0
12	37.09477772630193	38.0	35.0	40.0	32.0	41.0
13	36.944971343490586	38.0	35.0	40.0	31.0	41.0
14	36.84743140357165	38.0	35.0	40.0	31.0	41.0
15	36.76102481621664	38.0	35.0	40.0	31.0	41.0
16	36.73727502381972	38.0	35.0	40.0	31.0	41.0
17	36.72102225213649	38.0	35.0	40.0	31.0	41.0
18	36.60594750045891	38.0	35.0	40.0	31.0	41.0
19	36.525004152061605	38.0	35.0	40.0	31.0	41.0
20	36.37247926154492	38.0	35.0	40.0	30.0	41.0
21	36.226915265892195	38.0	34.0	40.0	30.0	41.0
22	36.21332855482032	38.0	34.0	40.0	30.0	41.0
23	36.246207055299635	38.0	34.0	40.0	30.0	41.0
24	36.22099457172577	38.0	34.0	40.0	30.0	41.0
25	36.19453536779025	38.0	34.0	40.0	30.0	41.0
26	36.166214318024906	38.0	34.0	40.0	30.0	41.0
27	36.21742435395529	38.0	34.0	40.0	30.0	41.0
28	36.18082136919137	38.0	34.0	40.0	30.0	41.0
29	36.17944939851674	37.0	34.0	40.0	30.0	41.0
30	36.23065323993536	37.0	34.0	40.0	31.0	41.0
31	36.16146730581979	37.0	34.0	40.0	31.0	41.0
32	36.14426596913353	37.0	34.0	40.0	31.0	41.0
33	36.07589272946254	37.0	34.0	40.0	31.0	41.0
34	36.024873181086505	37.0	34.0	40.0	31.0	41.0
35	35.91978694418287	37.0	34.0	40.0	30.0	41.0
36	35.836714019143606	36.0	34.0	40.0	30.0	41.0
37	35.76952814473967	36.0	34.0	39.0	30.0	41.0
38	35.68461909915911	36.0	34.0	39.0	30.0	41.0
39	35.565182436819036	36.0	34.0	39.0	30.0	41.0
40	35.449349862896256	36.0	34.0	39.0	30.0	41.0
41	35.29123152438059	35.0	33.0	39.0	30.0	40.0
42	35.15162464937192	35.0	33.0	39.0	29.0	40.0
43	35.02297313036696	35.0	33.0	39.0	29.0	40.0
44	34.91684273855652	35.0	33.0	39.0	29.0	40.0
45	34.85695328122159	35.0	33.0	38.0	29.0	40.0
46	34.80465822409835	35.0	33.0	38.0	29.0	40.0
47	34.757981216989094	35.0	33.0	38.0	29.0	40.0
48	34.76353678402403	35.0	33.0	38.0	29.0	40.0
49	34.78073554058755	35.0	33.0	38.0	29.0	40.0
50	34.977941435983105	35.0	33.0	38.0	29.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	2.0
21	3.0
22	11.0
23	32.0
24	75.0
25	167.0
26	451.0
27	982.0
28	2023.0
29	4103.0
30	7406.0
31	12545.0
32	19198.0
33	28030.0
34	37405.0
35	38064.0
36	45108.0
37	48404.0
38	45444.0
39	38680.0
40	15070.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	51.72361546956174	15.021139092607	33.255245437831256
2	26.75471951002759	22.262625909447177	31.40590262905627	19.576751951468957
3	25.715101557970065	17.61639612707348	27.883497521874805	28.785004793081647
4	19.82995486636189	20.044113833503786	31.88025745695696	28.24567384317736
5	21.049058428976437	21.26962759649537	28.306570746759206	29.37474322776899
6	24.975306159911188	20.675809943386277	27.372721100922774	26.976162795779757
7	29.754693286480595	19.71952459623022	24.531254097429215	25.994528019859963
8	26.005308811403165	18.19622788845086	26.21626267835654	29.582200621789433
9	27.42516819491671	19.349189838084165	25.556594785010624	27.669047181988503
10	26.009388029824915	20.02255225041739	26.657692386138816	27.310367333618878
11	29.443215822705515	20.27750340177679	25.24482594849113	25.03445482702657
12	28.988091595935934	19.54703193153906	24.51668546020868	26.948191012316325
13	27.01578948901962	19.436310288662977	25.483751598907933	28.06414862340947
14	27.490435689664714	21.56915877774961	22.287683965466503	28.652721567119166
15	25.346515036290473	21.74456516988488	24.428108145907814	28.48081164791683
16	27.791423734641015	22.536516289193276	22.57439474596667	27.097665230199038
17	29.30160866892189	21.9648429646594	20.197084524319425	28.53646384209928
18	26.90739882809882	21.382388848582327	22.213383915641764	29.49682840767709
19	28.1192180721031	20.35122070611271	24.49978584103286	27.029775380751335
20	28.24538247043295	20.29644263016349	24.701415780165092	26.756759119238467
21	28.54724463364248	24.10060518119014	21.829354638508406	25.522795546658973
22	30.002068746485318	21.741360069696363	22.73435838264817	25.522212801170152
23	28.435940245277575	24.131490692097678	22.154817994015204	25.27775106860954
24	30.354047021733493	23.4132568771252	21.01992115453536	25.212774946605943
25	29.00220671205796	24.124459738553234	22.43657544215262	24.43675810723619
26	29.496405018689142	23.697712068185606	22.802537913958528	24.003344999166725
27	29.36217262110428	23.738331235184013	22.199721782395375	24.699774361316333
28	29.098957511620288	23.347674122258766	23.05155402371388	24.50181434240707
29	29.848089865612064	23.234377823999903	23.157794775810785	23.759737534577248
30	28.07377492406522	24.369270130054723	22.68928075937868	24.867674186501375
31	26.758295258782113	23.322535140938257	25.66162334906249	24.25754625121714
32	26.53364432734953	22.023286778662285	25.34003166598991	26.10303722799828
33	26.462353939386237	21.19670396369657	26.357168052726408	25.98377404419078
34	26.611129281541196	20.403255285991882	28.0438142511752	24.941801181291726
35	26.78147864102489	21.346031252932594	25.588702605273973	26.28378750076854
36	26.853331503285915	22.20174312016549	23.829504939494058	27.11542043705454
37	25.47094816354131	23.588474922896772	26.351326835221744	24.58925007834018
38	27.379901756723	22.901007409874282	24.0	25.719090833402714
39	26.367645426381102	22.43130237048449	25.42409370259115	25.776958500543255
40	26.782178554661186	22.884417802393703	23.568235478230104	26.765168164715007
41	25.137512994031354	23.202004695061923	24.92995270516801	26.730529605738717
42	25.913201038381793	23.76988344338554	25.00409429064237	25.312821227590295
43	25.212913518111325	24.731144844722248	23.70619522749011	26.349746409676317
44	23.731387112253888	24.110986169702265	25.250857679002443	26.906769039041407
45	25.252863115797126	22.11706962370478	25.918196691510637	26.711870568987457
46	24.885580982004	23.351847737539806	24.100940531733688	27.661630748722505
47	25.962590242835265	23.48657686658124	24.80662249585899	25.744210394724504
48	23.98891011475581	23.60502947168767	24.622358551780778	27.783701861775747
49	26.39155045378364	23.37096417164722	23.35768269930114	26.879802675268
50	23.907954024070303	26.320866813331605	24.582063740426584	25.189115422171504
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1103.0
1	1069.0
2	717.0
3	389.5
4	372.5
5	278.5
6	156.0
7	135.0
8	150.0
9	149.0
10	144.5
11	148.5
12	159.0
13	176.0
14	202.5
15	232.0
16	259.5
17	303.0
18	351.5
19	398.0
20	470.5
21	591.5
22	772.5
23	1120.5
24	1504.5
25	1695.0
26	1723.5
27	1822.5
28	2069.0
29	2411.5
30	2796.0
31	3322.0
32	4002.5
33	4809.0
34	5731.5
35	6440.5
36	6936.5
37	7902.5
38	8906.0
39	9918.0
40	11338.5
41	12764.0
42	14657.0
43	16557.5
44	17641.0
45	18333.0
46	19731.0
47	21598.0
48	24004.0
49	26523.0
50	28120.0
51	29256.0
52	30771.0
53	31576.0
54	30520.0
55	28655.5
56	27005.5
57	25996.5
58	24958.5
59	22991.0
60	21520.0
61	21071.5
62	20296.5
63	19057.5
64	17306.0
65	15706.0
66	14432.5
67	13342.5
68	12140.0
69	10754.0
70	9413.0
71	8058.5
72	7140.5
73	6501.5
74	5549.5
75	4537.5
76	3768.5
77	2971.5
78	2311.0
79	1919.5
80	1574.5
81	1243.5
82	952.5
83	677.0
84	499.5
85	374.0
86	261.5
87	179.0
88	109.5
89	73.5
90	52.5
91	32.0
92	20.5
93	20.0
94	12.5
95	3.5
96	2.0
97	2.0
98	2.0
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	3784.0
25	3395.0
26	3191.0
27	3237.0
28	3153.0
29	2810.0
30	3209.0
31	2733.0
32	3010.0
33	2819.0
34	2835.0
35	3024.0
36	2838.0
37	2890.0
38	2994.0
39	3343.0
40	3420.0
41	3533.0
42	3654.0
43	4089.0
44	4192.0
45	4990.0
46	14084.0
47	35595.0
48	62266.0
49	73114.0
50	85001.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.611292380043686
#Duplication Level	Percentage of deduplicated	Percentage of total
1	56.298527506213446	17.233706860586313
2	14.05281393372538	8.603495921752389
3	7.819368175113861	7.180828963068571
4	5.0463638793061	6.179028806621221
5	3.5660959490952946	5.458140287652272
6	2.6537511000926215	4.874085049727868
7	1.9927912193971942	4.27013402685459
8	1.568112740287715	3.8401566062255004
9	1.1653677844249961	3.210607258138555
>10	5.519795782128136	27.47188488564538
>50	0.1874158581146745	3.9841923145485523
>100	0.1256085006513244	6.849356349574315
>500	0.0029906785869362952	0.4821556263010656
>1k	9.968928623120983E-4	0.36222704330339545
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ATTCCTGCTCACCGCAGTGTAGACAGGGAAACTTTCTTTGCAGTTCAGG	1187	0.34585944761555115	No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA	542	0.15792402747062234	No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCC	533	0.15530167277092566	No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTAG	505	0.14714323592742487	No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGT	486	0.14160715378362076	No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCCG	479	0.13956754457274556	No Hit
ATTCCTGCTCACCGCAGTGTAGACAGGGAAACTTTCTTTGCAGTTCA	467	0.13607107163981666	No Hit
ATTCCTGCTCACCGCAGTGTAGACAGGGAAACTTTCTTTGCAGTTCAGGA	448	0.13053498949601255	No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG	433	0.12616439832985143	No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG	387	0.11276125208695728	No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCGC	374	0.10897340640961763	No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG	369	0.10751654268756392	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.21969504928569972	0.0	0.0	0.0	0.0
2	0.5064058297858701	0.0	0.0	0.0	0.0
3	0.6788984944770297	0.0	0.0	0.0	0.0
4	0.8205056482606504	0.0	0.0	0.0	0.0
5	1.0367042246134213	0.0	0.0	0.0	0.0
6	1.1972506067837403	0.0	0.0	0.0	0.0
7	1.321958141391538	0.0	0.0	0.0	0.0
8	1.4280178203570482	0.0	0.0	0.0	0.0
9	1.5273759262011113	0.0	0.0	0.0	0.0
10	1.5961398938820466	0.0	0.0	0.0	0.0
11	1.6783070078058757	0.0	0.0	0.0	0.0
12	1.7476537209756324	0.0	0.0	0.0	0.0
13	1.792525123614887	0.0	0.0	0.0	0.0
14	1.8484686905417493	0.0	0.0	0.0	0.0
15	1.9163585399894523	0.0	0.0	0.0	0.0
16	1.9606471971398851	0.0	0.0	0.0	0.0
17	2.0063927180123717	0.0	0.0	0.0	0.0
18	2.0541778480957333	0.0	0.0	0.0	0.0
19	2.176845773492656	0.0	0.0	0.0	0.0
20	2.207148538911373	0.0	0.0	0.0	0.0
21	2.277369370314362	0.0	0.0	0.0	0.0
22	2.3003878171228105	0.0	0.0	0.0	0.0
23	2.3301078370527066	0.0	0.0	0.0	0.0
24	2.346424710739708	0.0	0.0	0.0	0.0
25	2.371191394014621	0.0	0.0	0.0	0.0
26	2.450444780494343	0.0	0.0	0.0	0.0
27	2.4702581271142736	0.0	0.0	0.0	0.0
28	2.4947334376447756	0.0	0.0	0.0	0.0
29	2.51192442956501	0.0	0.0	0.0	0.0
30	2.529698166974065	0.0	0.0	0.0	0.0
31	2.5463064134054774	0.0	0.0	0.0	0.0
32	2.5617491688592464	0.0	0.0	0.0	0.0
33	2.5742781968689084	0.0	0.0	0.0	0.0
34	2.5833107519456413	0.0	0.0	0.0	0.0
35	2.598753507399411	0.0	0.0	0.0	0.0
36	2.610991162664662	0.0	0.0	0.0	0.0
37	2.624394308907556	0.0	0.0	0.0	0.0
38	2.633426863984289	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCTACC	55	2.0008883E-10	101.04824	44
TCGCAAA	20	2.9061875E-5	85.66373	43
CGTGCGC	110	0.0	79.39504	44
ACGACAC	50	1.3335102E-6	79.39504	44
TCGTGCG	130	0.0	75.77946	43
CGCTATT	15	0.0034980187	62.431225	42
AATCCCG	90	0.0	61.86825	43
ACGCTAC	115	0.0	55.867645	43
ATCGGGA	25	1.3792127E-5	53.60728	41
CTCGCAA	35	6.5352833E-7	53.512478	42
ACCAGTA	45	0.00671022	52.930035	44
AATCCTG	75	1.03682396E-10	51.39824	43
AACGACA	85	5.456968E-12	50.39043	43
TACGCTA	115	0.0	48.85922	42
GTTCAGG	380	0.0	48.467636	43
GGGAACT	85	3.0999967E-5	46.70297	44
TTCAGGA	225	0.0	45.872692	44
CTAATCC	235	0.0	45.62322	41
TGTGTTA	185	0.0	45.557922	42
CGGGATC	45	7.661583E-9	44.383327	39
>>END_MODULE