FastQCFastQC Report
Tue 24 May 2016
ERR841176.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841176.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3260970
Sequences flagged as poor quality0
Sequence length24-50
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG381541.1700199633851276No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCGC197130.6045133809878656No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCC196130.6014468087716232No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGT186500.5719157183292088No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG170900.5240771917558272No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA147710.4529633820611658No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG146350.4487928438470762No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG137240.4208563709571078No Hit
ATTCCTGCTCACCGCAGTGTAGACAGGGAAACTTTCTTTGCAGTTCAGG133580.40963271664566064No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCCG126160.38687875080114204No Hit
ATCCGAATTCAGTCTTCCTGAGGCTCTGGCTCACCTCCAGGCAGGTCCC115580.35443441675329734No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA103230.3165622498827036No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTAG89700.2750715277969439No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTG85000.26065863838060455No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGC84540.25924801516113305No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA77550.23781267536959863No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATC72630.22272514006568597No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGAC71970.22070120240296598No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC71660.21975056501593085No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA71470.21916791629484478No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAAT71330.21873859618457087No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTC68930.21137882286558907No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCG67640.2074229447066364No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG59660.18295169842102196No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGA57020.17485594777014202No Hit
AGGCCTGGTTTGCGCTGGAGAGTGGTCTTGGTTGTGAGGGTCATAAGAT55370.16979610361334205No Hit
ATTCCTGCTCACCGCAGTGTAGACAGGGAAACTTTCTTTGCAGTTCA52910.16225233596138572No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTGC51160.15688583458296151No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCC49270.15109001309426337No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAAG49020.15032337004020277No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTG48020.14725679782396037No Hit
ATCCGAATTCAGTCTTCCTGAGGCTCTGGCTCACCTCCAGGCAGGTCCCA48000.14719546637963551No Hit
ATTCCTGCTCACCGCAGTGTAGACAGGGAAACTTTCTTTGCAGTTCAGGA45850.14060233611471434No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGGTTGTGAGGGTTCCTGCTT45820.14051033894822706No Hit
ATCCGAATTCAGTCTTCCTGAGGCTCTGGCTCACCTCCAGGCAGGTC45650.13998902167146585No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGGTTGTGAGGG45070.13821040978604524No Hit
CTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAATCCT45020.13805708117523313No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGT41260.12652676964216167No Hit
CTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAATCCTG40350.1237361889253811No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGT39550.12128293115238717No Hit
AGGCCTGGTTTGCGCTGGAGAGTGGTCTTGGTTGTGAGGGTCATAAGATG39390.12079227959778838No Hit
CGGCTCCTGGCCAGGTAACTTACTACTTCCAGTCAATATGCCGACCTT37520.11505778955341509No Hit
AAACAGCCGGGGCTCCAGCGGGAGAACGATAATGCAAAGTGCTATGTTCT35740.1095992910085036No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTT35480.10880198223228058No Hit
TCTGGAAGAGGTCTTGCGAAAGCCGCCTCGAGCGCGCTCCGCGGCTGC34440.10561274712738847No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCGC29800.0182.0684444
TAAGCCA13150.0162.8136944
CCAGAAT14750.0146.9556744
ATGTTCT7200.0144.0631644
CTATCAT5400.0128.0561444
GGTCCCA11700.0114.79587644
CTATACG1100.0108.8029344
CGCTACC2750.0106.38508644
AATGAAG16050.097.76823444
TCACGTC1650.096.7137244
ACTTCTA3200.095.5803544
TCGTGCG66700.089.4657843
TTCAGGA16100.087.5529544
CCTCGAA1800.081.26639644
GATACCC1750.075.9893544
TTAAGCC10500.074.6464843
GAACACA8350.073.25918644
AGGTCCC22550.071.8611843
ACGACAC1303.097739E-971.6053544
AATCCCG18700.070.1991943
Produced by FastQC (version 0.11.5)