##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841176.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3260970
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	56
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.44487345789749	37.0	35.0	39.0	30.0	39.0
2	36.41826971729271	37.0	35.0	39.0	32.0	39.0
3	36.61108811181949	38.0	35.0	39.0	32.0	39.0
4	36.92127403809296	38.0	35.0	40.0	32.0	41.0
5	37.31352573007418	38.0	36.0	40.0	32.0	41.0
6	37.311325771166246	38.0	36.0	40.0	32.0	41.0
7	37.24736259456542	38.0	36.0	40.0	32.0	41.0
8	36.97279705118416	38.0	35.0	40.0	31.0	41.0
9	36.87888695694839	38.0	35.0	40.0	31.0	41.0
10	36.6978061742365	38.0	35.0	40.0	31.0	41.0
11	36.388899008577205	38.0	35.0	40.0	30.0	41.0
12	36.32406461880974	38.0	34.0	40.0	30.0	41.0
13	36.11887843187763	38.0	34.0	40.0	30.0	41.0
14	36.12738663649159	38.0	34.0	40.0	30.0	40.0
15	36.19342833574059	38.0	34.0	40.0	30.0	41.0
16	36.04600195647308	38.0	34.0	40.0	30.0	40.0
17	36.10254709488281	38.0	34.0	40.0	30.0	41.0
18	35.891891369745814	37.0	34.0	40.0	30.0	40.0
19	35.752507076115386	37.0	34.0	39.0	30.0	40.0
20	35.65278674750151	37.0	33.0	39.0	30.0	40.0
21	35.37211749878104	37.0	33.0	39.0	29.0	40.0
22	35.48259413610061	37.0	33.0	39.0	29.0	40.0
23	35.541144199425325	37.0	33.0	39.0	29.0	40.0
24	35.43770871857147	37.0	33.0	39.0	29.0	40.0
25	35.39170544818062	37.0	33.0	39.0	29.0	40.0
26	35.31217824161704	36.0	33.0	39.0	29.0	40.0
27	35.27041738883942	36.0	33.0	39.0	29.0	40.0
28	35.28584019037298	36.0	33.0	39.0	29.0	40.0
29	35.289114827647275	36.0	33.0	39.0	29.0	40.0
30	35.2687887378726	36.0	33.0	39.0	30.0	40.0
31	35.08888596544556	35.0	33.0	39.0	29.0	40.0
32	35.027619868989774	35.0	33.0	39.0	29.0	40.0
33	34.967048582692215	35.0	33.0	39.0	29.0	40.0
34	34.87976556905325	35.0	33.0	39.0	29.0	40.0
35	34.76398860418645	35.0	33.0	38.0	29.0	40.0
36	34.599088726976966	35.0	33.0	38.0	29.0	40.0
37	34.54496472630568	35.0	33.0	38.0	29.0	40.0
38	34.508560800458355	35.0	33.0	38.0	29.0	40.0
39	34.28860276232423	35.0	33.0	37.0	29.0	40.0
40	34.088600269050175	35.0	33.0	37.0	28.0	40.0
41	33.96860083061767	35.0	33.0	37.0	28.0	40.0
42	33.837346573804794	35.0	32.0	36.0	28.0	39.0
43	33.77236294040914	35.0	32.0	36.0	28.0	39.0
44	33.66725263960792	34.0	32.0	36.0	28.0	39.0
45	33.654477904412786	34.0	32.0	36.0	28.0	39.0
46	33.47247748806523	34.0	32.0	35.0	28.0	39.0
47	33.50329441434873	34.0	32.0	35.0	28.0	39.0
48	33.54374301998186	34.0	32.0	35.0	28.0	39.0
49	33.60537066823739	34.0	32.0	36.0	28.0	39.0
50	34.092437767096754	35.0	33.0	37.0	29.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	7.0
21	16.0
22	73.0
23	211.0
24	679.0
25	1881.0
26	5063.0
27	12513.0
28	28333.0
29	57278.0
30	104121.0
31	169670.0
32	249323.0
33	336107.0
34	414691.0
35	455129.0
36	481213.0
37	432353.0
38	303881.0
39	170613.0
40	37815.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	51.78609432162823	14.398568524089459	33.81533715428232
2	28.91789866205454	21.03177888787692	26.899787486545414	23.15053496352312
3	30.512761540277893	16.476385860648826	23.17298227214602	29.837870326927263
4	20.404787532543995	17.572010782067913	28.69081898944179	33.3323826959463
5	24.126716897119568	18.18173733582339	24.734450178934487	32.957095588122556
6	29.616279818581585	19.127069552924436	24.680785165150247	26.575865463343728
7	36.19775710908104	19.679911192068616	18.799283648730285	25.323048050120057
8	28.513111129510545	15.458222553412021	29.246788532246544	26.781877784830893
9	29.971818201332734	17.3492243105579	24.652971355148928	28.02598613296044
10	31.75680855696311	18.30354158425254	26.165404772199686	23.774245086584667
11	34.85711306758418	16.87037905899165	24.877965758654632	23.394542114769532
12	35.58569996044122	16.7989279263532	20.792126269177576	26.823245844028005
13	30.076510976795245	16.518029911345398	27.774189888284774	25.63126922357458
14	27.969990524291855	18.12761233620671	20.37277251860643	33.52962462089501
15	26.34225399191038	17.683358019239673	24.154929361508998	31.819458627340946
16	27.718654265448624	22.812752033904022	25.342244792193735	24.126348908453622
17	28.57180532172943	19.661757084548462	16.666421340889368	35.100016252832745
18	26.84492650959684	21.10779921311757	23.367985599376873	28.679288677908723
19	30.360444898297136	16.810458237886273	26.12284074983824	26.706256113978355
20	29.9491562326547	16.502421058764725	25.03862347706357	28.509799231517004
21	32.493767191970484	21.16091224390289	19.849891290014934	26.495429274111693
22	31.060819326764737	19.584326136088343	21.63294970514908	27.72190483199784
23	31.554384124968948	23.372248134757452	19.251511053459552	25.821856686814048
24	33.727694520342105	21.001971805935042	17.771399307567997	27.498934366154852
25	28.768211377849017	23.766754064105044	22.361431811529446	25.10360274651649
26	32.807880380572996	19.80767146440807	21.31881078007411	26.065637374944828
27	28.51745004534832	20.250899816127983	22.78347783144154	28.448172307082153
28	29.642453252274713	21.247471066820363	24.235426294866784	24.874649386038136
29	35.15094526891981	19.08338463554455	22.9640374202329	22.801632675302738
30	28.10352340912596	25.80034396922677	19.96805859721714	26.12807402443013
31	29.15968611520559	20.540013135562393	25.60358307844562	24.6967176707864
32	26.939884292195764	19.935736529839726	26.199461730516255	26.924917447448255
33	25.789091511497368	18.360416883786566	27.45097107334913	28.39952053136694
34	25.363397491045088	17.211800093788856	29.474521494881778	27.950280920284275
35	31.615222890238037	17.3944142458086	24.830095781083713	26.160267082869648
36	27.795654950684195	18.354140956636325	21.593121480925866	32.25708261175362
37	25.809178263562544	19.4474829669112	27.241470670129164	27.501868099397093
38	29.50896825248671	19.364669206391145	21.139759994600812	29.98660254652133
39	28.92961751204941	20.710061964238776	25.486332870090024	24.873987653621793
40	28.13116895452581	18.470839276023828	24.05182639710598	29.34616537234438
41	27.298036623468626	23.159131550295964	21.65324045026689	27.88959137596852
42	25.0374450147529	21.19639423140584	25.624988413704706	28.141172340136556
43	25.16000477700708	23.983417180559734	25.968755148050736	24.887822894382445
44	23.399818124813795	22.315937164150935	23.536653465377423	30.747591245657844
45	28.4808813690832	18.04973733902861	27.55984592667662	25.90953536521157
46	23.528153089261544	20.34321075709805	26.444532700054303	29.684103453586104
47	32.71876155608172	18.647068720458055	22.403507265325008	26.230662458135217
48	23.721050948233827	21.817242807632013	23.55725318918012	30.904453054954047
49	31.204926636611464	20.666576047967496	20.294510772297862	27.833986543123174
50	24.977607427979954	23.82282710064872	22.65308423091507	28.546481240456256
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1071.0
1	971.5
2	689.0
3	502.0
4	467.0
5	389.5
6	301.0
7	269.5
8	290.5
9	315.0
10	364.5
11	448.5
12	518.0
13	537.0
14	520.5
15	521.5
16	557.0
17	650.0
18	747.0
19	899.5
20	1123.5
21	1461.0
22	1890.5
23	2585.5
24	3398.5
25	4012.0
26	4736.5
27	5884.0
28	7343.5
29	8992.0
30	11097.0
31	14327.0
32	18165.5
33	21177.0
34	25862.5
35	33059.5
36	40080.0
37	45385.0
38	48640.5
39	55762.5
40	66988.0
41	85320.5
42	112633.5
43	133390.0
44	137209.0
45	130468.5
46	135128.0
47	150258.0
48	172898.5
49	206026.5
50	240412.0
51	257406.5
52	258441.5
53	266814.0
54	267992.0
55	265053.0
56	269663.0
57	285557.5
58	299802.0
59	290770.0
60	284752.0
61	295968.0
62	305859.0
63	298607.5
64	257975.0
65	209414.5
66	180089.5
67	162922.0
68	150449.0
69	136355.5
70	119823.5
71	104905.0
72	94651.5
73	85004.0
74	71838.5
75	59038.0
76	50615.0
77	40852.0
78	32165.0
79	25445.0
80	19138.5
81	14312.5
82	10845.5
83	8203.0
84	6334.0
85	4754.5
86	3391.5
87	2301.0
88	1456.5
89	1043.5
90	726.5
91	413.5
92	274.5
93	247.0
94	157.5
95	56.5
96	37.0
97	36.0
98	19.5
99	3.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	39289.0
25	38586.0
26	36341.0
27	33287.0
28	30429.0
29	28695.0
30	44194.0
31	30229.0
32	34159.0
33	32835.0
34	32921.0
35	34974.0
36	29335.0
37	30092.0
38	31112.0
39	38270.0
40	37011.0
41	36002.0
42	39068.0
43	39745.0
44	38326.0
45	58490.0
46	185232.0
47	375118.0
48	583000.0
49	836345.0
50	487885.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.590393083278936
#Duplication Level	Percentage of deduplicated	Percentage of total
1	57.388890215829605	11.242709179412426
2	13.421017236296956	5.258460064730386
3	7.076133078267384	4.1587268553855194
4	4.6316214952685995	3.6294114282110406
5	3.300989235509868	3.233383834365537
6	2.435531873916099	2.8627816066122804
7	1.8205802690458246	2.49661181771886
8	1.5292108553187516	2.3966273410329233
9	1.1597482361131906	2.044793144078712
>10	6.479251114027409	22.020071480926642
>50	0.4071304139501889	5.522903800738841
>100	0.2906522981808959	11.377225488869062
>500	0.03123448940588793	4.187962035122802
>1k	0.02325611439460134	9.090977667202825
>5k	0.0027160425570337335	3.6864734181515963
>10k+	0.0020370319177752997	6.790880837440659
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG	38154	1.1700199633851276	No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCGC	19713	0.6045133809878656	No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCC	19613	0.6014468087716232	No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGT	18650	0.5719157183292088	No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG	17090	0.5240771917558272	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA	14771	0.4529633820611658	No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG	14635	0.4487928438470762	No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG	13724	0.4208563709571078	No Hit
ATTCCTGCTCACCGCAGTGTAGACAGGGAAACTTTCTTTGCAGTTCAGG	13358	0.40963271664566064	No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCCG	12616	0.38687875080114204	No Hit
ATCCGAATTCAGTCTTCCTGAGGCTCTGGCTCACCTCCAGGCAGGTCCC	11558	0.35443441675329734	No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA	10323	0.3165622498827036	No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTAG	8970	0.2750715277969439	No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTG	8500	0.26065863838060455	No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGC	8454	0.25924801516113305	No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA	7755	0.23781267536959863	No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATC	7263	0.22272514006568597	No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGAC	7197	0.22070120240296598	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC	7166	0.21975056501593085	No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA	7147	0.21916791629484478	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAAT	7133	0.21873859618457087	No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTC	6893	0.21137882286558907	No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCG	6764	0.2074229447066364	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG	5966	0.18295169842102196	No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGA	5702	0.17485594777014202	No Hit
AGGCCTGGTTTGCGCTGGAGAGTGGTCTTGGTTGTGAGGGTCATAAGAT	5537	0.16979610361334205	No Hit
ATTCCTGCTCACCGCAGTGTAGACAGGGAAACTTTCTTTGCAGTTCA	5291	0.16225233596138572	No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTGC	5116	0.15688583458296151	No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCC	4927	0.15109001309426337	No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAAG	4902	0.15032337004020277	No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTG	4802	0.14725679782396037	No Hit
ATCCGAATTCAGTCTTCCTGAGGCTCTGGCTCACCTCCAGGCAGGTCCCA	4800	0.14719546637963551	No Hit
ATTCCTGCTCACCGCAGTGTAGACAGGGAAACTTTCTTTGCAGTTCAGGA	4585	0.14060233611471434	No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGGTTGTGAGGGTTCCTGCTT	4582	0.14051033894822706	No Hit
ATCCGAATTCAGTCTTCCTGAGGCTCTGGCTCACCTCCAGGCAGGTC	4565	0.13998902167146585	No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGGTTGTGAGGG	4507	0.13821040978604524	No Hit
CTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAATCCT	4502	0.13805708117523313	No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGT	4126	0.12652676964216167	No Hit
CTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAATCCTG	4035	0.1237361889253811	No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGT	3955	0.12128293115238717	No Hit
AGGCCTGGTTTGCGCTGGAGAGTGGTCTTGGTTGTGAGGGTCATAAGATG	3939	0.12079227959778838	No Hit
CGGCTCCTGGCCAGGTAACTTACTACTTCCAGTCAATATGCCGACCTT	3752	0.11505778955341509	No Hit
AAACAGCCGGGGCTCCAGCGGGAGAACGATAATGCAAAGTGCTATGTTCT	3574	0.1095992910085036	No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTT	3548	0.10880198223228058	No Hit
TCTGGAAGAGGTCTTGCGAAAGCCGCCTCGAGCGCGCTCCGCGGCTGC	3444	0.10561274712738847	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0491878183485282	0.0	0.0	0.0	0.0
2	0.10742202473497149	0.0	0.0	0.0	0.0
3	0.15553654280781484	0.0	0.0	0.0	0.0
4	0.19681260483843765	0.0	0.0	0.0	0.0
5	0.24434447419019495	0.0	0.0	0.0	0.0
6	0.3062279015139667	0.0	0.0	0.0	0.0
7	0.35958625807658456	0.0	0.0	0.0	0.0
8	0.3943611870087735	0.0	0.0	0.0	0.0
9	0.4286454643863636	0.0	0.0	0.0	0.0
10	0.45740991177471735	0.0	0.0	0.0	0.0
11	0.48249447250358024	0.0	0.0	0.0	0.0
12	0.5028871777415922	0.0	0.0	0.0	0.0
13	0.5192320076541642	0.0	0.0	0.0	0.0
14	0.5370487922305326	0.0	0.0	0.0	0.0
15	0.552841639144181	0.0	0.0	0.0	0.0
16	0.567009202783221	0.0	0.0	0.0	0.0
17	0.5812380978665858	0.0	0.0	0.0	0.0
18	0.5973989334461832	0.0	0.0	0.0	0.0
19	0.6078559447035699	0.0	0.0	0.0	0.0
20	0.6171783242409468	0.0	0.0	0.0	0.0
21	0.6288926301069927	0.0	0.0	0.0	0.0
22	0.6371417093686847	0.0	0.0	0.0	0.0
23	0.6472307319601223	0.0	0.0	0.0	0.0
24	0.6557864684434386	0.0	0.0	0.0	0.0
25	0.6665501369224495	0.0	0.0	0.0	0.0
26	0.6755045277938773	0.0	0.0	0.0	0.0
27	0.6839989328328687	0.0	0.0	0.0	0.0
28	0.6944559440902554	0.0	0.0	0.0	0.0
29	0.7027356890741099	0.0	0.0	0.0	0.0
30	0.7123033943887862	0.0	0.0	0.0	0.0
31	0.7203071478731788	0.0	0.0	0.0	0.0
32	0.7280042441359473	0.0	0.0	0.0	0.0
33	0.7358546690095279	0.0	0.0	0.0	0.0
34	0.7428464536625605	0.0	0.0	0.0	0.0
35	0.7495009153718065	0.0	0.0	0.0	0.0
36	0.7563700371361896	0.0	0.0	0.0	0.0
37	0.7626871759016489	0.0	0.0	0.0	0.0
38	0.7679923458357483	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTGCGC	2980	0.0	182.06844	44
TAAGCCA	1315	0.0	162.81369	44
CCAGAAT	1475	0.0	146.95567	44
ATGTTCT	720	0.0	144.06316	44
CTATCAT	540	0.0	128.05614	44
GGTCCCA	1170	0.0	114.795876	44
CTATACG	110	0.0	108.80293	44
CGCTACC	275	0.0	106.385086	44
AATGAAG	1605	0.0	97.768234	44
TCACGTC	165	0.0	96.71372	44
ACTTCTA	320	0.0	95.58035	44
TCGTGCG	6670	0.0	89.46578	43
TTCAGGA	1610	0.0	87.55295	44
CCTCGAA	180	0.0	81.266396	44
GATACCC	175	0.0	75.98935	44
TTAAGCC	1050	0.0	74.64648	43
GAACACA	835	0.0	73.259186	44
AGGTCCC	2255	0.0	71.86118	43
ACGACAC	130	3.097739E-9	71.60535	44
AATCCCG	1870	0.0	70.19919	43
>>END_MODULE