FastQCFastQC Report
Tue 24 May 2016
ERR841175.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841175.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12002686
Sequences flagged as poor quality0
Sequence length24-50
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG436080.36331867716942695No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGA411350.34271495563576354No Hit
ACTACTTCTCCCCCGGACTCCTTGGTAGTCTGTTAGTGGGAGATCCTTG366420.30528166778669374No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGAC268570.22375824877864836No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGT244600.20378771884893096No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTG223380.18610834274928129No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG200040.16666269533336123No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG198920.1657295708643882No Hit
AGCGAGCGGCCTTGAGAGGCTCTGGCTCTTGCTTCTTAGGCGGCCCGAG188910.15738977092294174No Hit
ATCCGAATTCAGTCTTCCTGAGGCTCTGGCTCACCTCCAGGCAGGTCCC180260.15018305069381968No Hit
ACCAGCGAGCGGCCTTGAGAGGCTCTGGCTCTTGCTTCTTAGGCGGCCC175800.14646721575487354No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGG162840.13566963261389992No Hit
ACTACTTCTCCCCCGGACTCCTTGGTAGTCTGTTAGTGGGAGATCCT158820.13232038228776458No Hit
ACTACTTCTCCCCCGGACTCCTTGGTAGTCTGTTAGTGGGAGATCCTTGT136970.11411612367431757No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCC135960.11327464535854724No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG133530.11125009851961469No Hit
AAAACAGCCGGGGCTCCAGCGGGAGAACGATAATGCAAAGTGCTATGTT132820.11065856425803357No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGGTTGTGAGGGTTCCTGCTT132190.11013368174423625No Hit
CTCTTTCCCTTCGGTGTGCCACTGAAGATCCTGGTGTCGCCATGGGCCG127780.10645950414765494No Hit
AAACAGCCGGGGCTCCAGCGGGAGAACGATAATGCAAAGTGCTATGTTC122990.10246873074910066No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG3550.0114.5522144
ATGTTCT33300.0102.3283344
CGTGCGC41350.083.7635944
TCGTGCG72500.078.7539943
ATCCTTG74850.069.0656143
TCCTTGT58400.066.27244
CCCGAGG42450.063.0942144
CTATCAT15650.060.9296144
TTTGGAC51750.058.80007643
ACTTCTA12900.058.6999744
CTCGTGC84750.053.87117842
CCCATAC6000.053.75395644
AGGTCCC47650.053.435543
TATGTTC49400.050.761643
ACGACAC4150.050.6847144
TCGGATA1402.067927E-550.0813344
GCCCGAG60300.047.02392243
GATCCTT87650.046.61180542
GTTTGGA109350.045.89650742
TGTGTTA32000.044.9004942
Produced by FastQC (version 0.11.5)