##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841175.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	12002686
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.04991224464258	38.0	35.0	39.0	31.0	39.0
2	36.84407740067515	38.0	35.0	39.0	33.0	39.0
3	37.08830048540801	39.0	37.0	39.0	33.0	39.0
4	37.65657412015944	39.0	37.0	40.0	33.0	41.0
5	38.05461294246971	39.0	37.0	40.0	33.0	41.0
6	38.096459075910175	39.0	37.0	41.0	33.0	41.0
7	38.01405385427895	39.0	37.0	41.0	33.0	41.0
8	37.94380982723367	39.0	37.0	40.0	33.0	41.0
9	37.891898446731005	39.0	37.0	40.0	33.0	41.0
10	37.696320057027236	39.0	36.0	40.0	33.0	41.0
11	37.61089976027033	39.0	36.0	40.0	33.0	41.0
12	37.47335429752974	39.0	36.0	40.0	32.0	41.0
13	37.33110922005291	39.0	36.0	40.0	32.0	41.0
14	37.29634233537393	39.0	36.0	40.0	32.0	41.0
15	37.249417921955136	38.0	36.0	40.0	32.0	41.0
16	37.211969304204075	38.0	36.0	40.0	32.0	41.0
17	37.10736813409932	38.0	35.0	40.0	31.0	41.0
18	36.989734714379765	38.0	35.0	40.0	31.0	41.0
19	36.90641944644724	38.0	35.0	40.0	31.0	41.0
20	36.76219947768358	38.0	35.0	40.0	31.0	41.0
21	36.54902619297047	38.0	35.0	40.0	30.0	41.0
22	36.60697588856361	38.0	35.0	40.0	30.0	41.0
23	36.554066398137884	38.0	35.0	40.0	30.0	41.0
24	36.51328544294169	38.0	35.0	40.0	30.0	41.0
25	36.47013051166761	38.0	35.0	40.0	30.0	41.0
26	36.465587382590144	38.0	35.0	40.0	31.0	41.0
27	36.492883904515324	38.0	35.0	40.0	31.0	41.0
28	36.454511206665934	38.0	35.0	40.0	31.0	41.0
29	36.460297015156144	38.0	35.0	40.0	31.0	41.0
30	36.459906517032856	38.0	35.0	40.0	31.0	41.0
31	36.358543630959076	38.0	34.0	40.0	31.0	41.0
32	36.31119435851497	38.0	34.0	40.0	31.0	41.0
33	36.224236708134804	37.0	34.0	40.0	31.0	41.0
34	36.16346590218497	37.0	34.0	40.0	31.0	41.0
35	36.06497730019846	37.0	34.0	40.0	30.0	41.0
36	35.96645530362711	37.0	34.0	40.0	30.0	41.0
37	35.881551621378236	37.0	34.0	40.0	30.0	41.0
38	35.812367788272496	36.0	34.0	40.0	30.0	41.0
39	35.66435258348541	36.0	34.0	39.0	30.0	41.0
40	35.4768997236806	36.0	34.0	39.0	30.0	41.0
41	35.293268259119394	35.0	33.0	39.0	29.0	41.0
42	35.16848432332533	35.0	33.0	39.0	29.0	40.0
43	35.01133387779584	35.0	33.0	39.0	29.0	40.0
44	34.93997406137881	35.0	33.0	39.0	29.0	40.0
45	34.8576249377576	35.0	33.0	38.0	29.0	40.0
46	34.76231350019578	35.0	33.0	38.0	29.0	40.0
47	34.7052028433518	35.0	33.0	38.0	29.0	40.0
48	34.715995614590135	35.0	33.0	38.0	29.0	40.0
49	34.78225884637953	35.0	33.0	38.0	29.0	40.0
50	35.39344643692208	36.0	33.0	39.0	30.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	3.0
21	18.0
22	125.0
23	391.0
24	1120.0
25	3132.0
26	8091.0
27	20366.0
28	49205.0
29	107850.0
30	212166.0
31	372923.0
32	591272.0
33	865457.0
34	1184103.0
35	1347276.0
36	1611249.0
37	1760013.0
38	1723335.0
39	1484749.0
40	659840.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	49.91542726353085	18.51046507423422	31.574107662234933
2	29.14001082757643	23.30580005175508	25.533884665482375	22.020304455186114
3	27.80026070831146	17.065988396263972	27.00696327472034	28.12678762070423
4	21.935106858581488	18.32225720142975	30.711842332624546	29.03079360736422
5	23.708718198576552	20.205793936457223	27.008146343243506	29.077341521722722
6	28.120330732637676	18.39010868067364	25.092608437811336	28.39695214887734
7	31.685416081033864	18.450603473255903	23.30175928954569	26.562221156164544
8	30.11536751023896	17.190235585601425	25.099148640562618	27.595248263597
9	29.823891085711978	17.13069058042508	24.358439435972915	28.686978897890018
10	28.393202988064502	17.464215926335154	26.45851103661297	27.68407004898737
11	32.76196677976913	18.184729651346373	23.12660682783837	25.92669674104613
12	32.30505238577432	17.277524380792766	23.694179786091212	26.723243447341705
13	28.822981789242842	17.324405553890188	24.102288437771346	29.750324219095624
14	28.20246234884425	19.66108252769422	21.712964914686598	30.423490208774933
15	27.437641874493757	20.042672115224875	23.771712431700703	28.74797357858066
16	29.78513309437571	19.270211684284668	23.463206485614972	27.481448735724655
17	29.459664278479003	19.910085125945976	19.48038963945237	31.149860956122655
18	28.871046030863422	19.48507192473418	21.821174027213573	29.82270801718882
19	29.687388306250785	18.623264825889805	24.090216139954006	27.5991307279054
20	30.599584126419703	18.06487314589418	22.728487606857332	28.60705512082879
21	30.92549451014548	20.859755891306328	22.245620688569208	25.969128909978984
22	31.821418972386677	18.742488139738057	22.385406066608756	27.050686821266506
23	30.307291217982375	20.935855524338468	23.913214092245685	24.84363916543347
24	35.21166012340905	19.988484244276655	20.678371491181224	24.121484141133077
25	31.721936993536858	21.386966787108616	21.87034863603219	25.02074758332234
26	30.005640910871907	21.84180416634922	21.968201724550767	26.184353198228106
27	30.89812683838151	21.269763606844144	22.309249589816588	25.52285996495775
28	31.289878358853894	21.374579505913008	22.026429238216227	25.309112897016874
29	31.288913066259816	19.828982003814406	23.78946959931106	25.09263533061472
30	30.079276445004993	21.38719246358533	23.121559564345066	25.411971527064615
31	29.80974628329658	20.173588521105806	24.77557595912516	25.24108923647245
32	29.398246563037922	19.617463430123923	24.502775068033795	26.481514938804363
33	27.588596579444957	19.107361432367618	27.666685692903847	25.637356295283574
34	27.924987822698487	18.52610329093287	28.203516749843953	25.345392136524687
35	29.0637125989454	18.760233167537233	24.474984391782982	27.701069841734384
36	28.069585545727517	20.86564076410833	24.091347547563664	26.973426142600488
37	26.763393219647497	20.919659503858835	27.316536577594913	25.000410698898754
38	28.158722103479466	20.899359054410542	22.71022979329159	28.231689048818403
39	28.532053300752636	21.45470791960353	24.661975727341044	25.351263052302798
40	29.090126826546143	19.965380929343617	24.068647957704282	26.875844286405957
41	26.800369705695843	21.500914952987983	24.75142388639881	26.947291454917373
42	28.40257020597749	20.25161034810471	24.777623556774724	26.56819588914307
43	25.3789776080987	22.593493320773458	25.48529372544698	26.542235345680858
44	25.325876388096003	21.653593225903666	24.861118937888598	28.159411448111733
45	27.19462250527687	20.115863116054662	25.397035002099965	27.292479376568497
46	27.45034723458209	20.173973255224624	23.887484807957325	28.488194702235965
47	28.07503951463991	19.985275318871814	24.931121111594955	27.00856405489332
48	24.864345604834277	22.381377442509216	25.1550320828764	27.599244869780104
49	28.202254081811716	20.87568870167956	22.870532686372222	28.0515245301365
50	24.784344777802527	24.27839902600924	25.245392727489	25.691863468699232
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2007.0
1	2251.0
2	3363.5
3	4226.0
4	4121.0
5	3379.5
6	2491.0
7	2397.0
8	2680.5
9	2902.5
10	3043.5
11	3236.0
12	3403.0
13	3589.0
14	3934.5
15	4526.5
16	5206.0
17	6149.0
18	7519.5
19	9777.5
20	12161.0
21	15238.0
22	18963.5
23	23519.5
24	28548.0
25	33420.0
26	39466.0
27	47454.0
28	56665.0
29	65394.5
30	74219.5
31	85945.0
32	100899.0
33	114845.0
34	130603.0
35	148674.0
36	168666.5
37	188562.5
38	206310.5
39	235632.0
40	273162.0
41	319723.0
42	382557.0
43	434750.0
44	477155.5
45	521186.0
46	572067.0
47	612128.5
48	664887.5
49	742039.0
50	849050.0
51	966212.0
52	1072244.0
53	1140407.0
54	1093183.5
55	1005433.0
56	953720.0
57	947125.0
58	960018.5
59	937090.5
60	893789.0
61	863847.5
62	837800.0
63	802299.0
64	757068.0
65	713459.0
66	657899.5
67	601932.5
68	541852.5
69	478786.5
70	434881.5
71	403428.5
72	368240.5
73	327243.0
74	283126.0
75	242845.0
76	211727.5
77	171091.5
78	135549.5
79	109975.5
80	85657.0
81	63859.5
82	47552.5
83	35650.0
84	26366.5
85	19988.5
86	15168.5
87	10933.5
88	7778.5
89	5547.0
90	3747.5
91	2329.0
92	1616.5
93	1294.0
94	874.5
95	431.0
96	266.5
97	244.0
98	143.5
99	42.5
100	42.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	127809.0
25	114365.0
26	106579.0
27	107100.0
28	105464.0
29	87429.0
30	114948.0
31	97103.0
32	104499.0
33	94900.0
34	97241.0
35	99914.0
36	92762.0
37	98479.0
38	103735.0
39	121370.0
40	126279.0
41	125541.0
42	140764.0
43	140973.0
44	147668.0
45	208612.0
46	744906.0
47	1606197.0
48	2147412.0
49	3225631.0
50	1715006.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.179117287873822
#Duplication Level	Percentage of deduplicated	Percentage of total
1	56.24618923319195	10.787522602993342
2	12.566195284308513	4.820170664401598
3	6.904194166307305	3.972490490815861
4	4.759858511330313	3.6515953864995425
5	3.4101373232443297	3.2701711845129546
6	2.5879660186167746	2.9780942284849785
7	2.073851252075572	2.784224548081297
8	1.6786581190507048	2.5756144761212103
9	1.3185895267353702	2.2760444869037735
>10	7.652172489194976	25.603041759978783
>50	0.4425276889032744	5.843374177215178
>100	0.3012763295883457	11.309474450496943
>500	0.03257526101596494	4.335501646758384
>1k	0.022336340500861807	8.568680452362399
>5k	0.0023462540107804	3.093812806544647
>10k+	0.001126201925174592	4.130186637829043
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG	43608	0.36331867716942695	No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGA	41135	0.34271495563576354	No Hit
ACTACTTCTCCCCCGGACTCCTTGGTAGTCTGTTAGTGGGAGATCCTTG	36642	0.30528166778669374	No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGAC	26857	0.22375824877864836	No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGT	24460	0.20378771884893096	No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTG	22338	0.18610834274928129	No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG	20004	0.16666269533336123	No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG	19892	0.1657295708643882	No Hit
AGCGAGCGGCCTTGAGAGGCTCTGGCTCTTGCTTCTTAGGCGGCCCGAG	18891	0.15738977092294174	No Hit
ATCCGAATTCAGTCTTCCTGAGGCTCTGGCTCACCTCCAGGCAGGTCCC	18026	0.15018305069381968	No Hit
ACCAGCGAGCGGCCTTGAGAGGCTCTGGCTCTTGCTTCTTAGGCGGCCC	17580	0.14646721575487354	No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGG	16284	0.13566963261389992	No Hit
ACTACTTCTCCCCCGGACTCCTTGGTAGTCTGTTAGTGGGAGATCCT	15882	0.13232038228776458	No Hit
ACTACTTCTCCCCCGGACTCCTTGGTAGTCTGTTAGTGGGAGATCCTTGT	13697	0.11411612367431757	No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCC	13596	0.11327464535854724	No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG	13353	0.11125009851961469	No Hit
AAAACAGCCGGGGCTCCAGCGGGAGAACGATAATGCAAAGTGCTATGTT	13282	0.11065856425803357	No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGGTTGTGAGGGTTCCTGCTT	13219	0.11013368174423625	No Hit
CTCTTTCCCTTCGGTGTGCCACTGAAGATCCTGGTGTCGCCATGGGCCG	12778	0.10645950414765494	No Hit
AAACAGCCGGGGCTCCAGCGGGAGAACGATAATGCAAAGTGCTATGTTC	12299	0.10246873074910066	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.1760605917708753	0.0	0.0	0.0	0.0
2	0.4656707673599059	0.0	0.0	0.0	0.0
3	0.6022235356319411	0.0	0.0	0.0	0.0
4	0.714315112467326	0.0	0.0	0.0	0.0
5	0.985937647623207	0.0	0.0	0.0	0.0
6	1.1534334898038656	0.0	0.0	0.0	0.0
7	1.3007505153429824	0.0	0.0	0.0	0.0
8	1.4165995844596784	0.0	0.0	0.0	0.0
9	1.5126030956737517	0.0	0.0	0.0	0.0
10	1.5956095160699864	0.0	0.0	0.0	0.0
11	1.6802655672238698	0.0	0.0	0.0	0.0
12	1.7406103933736166	0.0	0.0	0.0	0.0
13	1.785408699352795	0.0	0.0	0.0	0.0
14	1.8359807129837438	1.0830909014865506E-4	0.0	0.0	0.0
15	1.901557701334518	1.0830909014865506E-4	0.0	0.0	0.0
16	1.936374908083074	1.0830909014865506E-4	0.0	0.0	0.0
17	1.978790414078982	1.0830909014865506E-4	0.0	0.0	0.0
18	2.0122579229349165	1.0830909014865506E-4	0.0	0.0	0.0
19	2.1392794912738697	1.0830909014865506E-4	0.0	0.0	0.0
20	2.165948521855858	1.0830909014865506E-4	0.0	0.0	0.0
21	2.2310339535667265	1.0830909014865506E-4	0.0	0.0	0.0
22	2.249504819171309	1.0830909014865506E-4	0.0	0.0	0.0
23	2.2691087644882155	1.0830909014865506E-4	0.0	0.0	0.0
24	2.286163280452392	1.0830909014865506E-4	0.0	0.0	0.0
25	2.3098996341318934	1.1664055862162852E-4	0.0	0.0	0.0
26	2.445136030385199	1.1664055862162852E-4	0.0	0.0	0.0
27	2.463356951935592	1.6662936945946932E-4	0.0	0.0	0.0
28	2.4813279294317954	2.4161258571623053E-4	0.0	0.0	0.0
29	2.497515972674783	2.4161258571623053E-4	0.0	0.0	0.0
30	2.511587822925635	2.4161258571623053E-4	0.0	0.0	0.0
31	2.5259262801676226	2.4161258571623053E-4	0.0	0.0	0.0
32	2.5403397206258664	2.4161258571623053E-4	0.0	0.0	0.0
33	2.5547198352102187	2.4161258571623053E-4	0.0	0.0	0.0
34	2.5670754029556386	2.4161258571623053E-4	0.0	0.0	0.0
35	2.581388865792207	2.4161258571623053E-4	0.0	0.0	0.0
36	2.594444276889356	2.4161258571623053E-4	0.0	0.0	0.0
37	2.6053668320574244	2.4161258571623053E-4	0.0	0.0	0.0
38	2.6128651536831007	2.4161258571623053E-4	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATACG	355	0.0	114.55221	44
ATGTTCT	3330	0.0	102.32833	44
CGTGCGC	4135	0.0	83.76359	44
TCGTGCG	7250	0.0	78.75399	43
ATCCTTG	7485	0.0	69.06561	43
TCCTTGT	5840	0.0	66.272	44
CCCGAGG	4245	0.0	63.09421	44
CTATCAT	1565	0.0	60.92961	44
TTTGGAC	5175	0.0	58.800076	43
ACTTCTA	1290	0.0	58.69997	44
CTCGTGC	8475	0.0	53.871178	42
CCCATAC	600	0.0	53.753956	44
AGGTCCC	4765	0.0	53.4355	43
TATGTTC	4940	0.0	50.7616	43
ACGACAC	415	0.0	50.68471	44
TCGGATA	140	2.067927E-5	50.08133	44
GCCCGAG	6030	0.0	47.023922	43
GATCCTT	8765	0.0	46.611805	42
GTTTGGA	10935	0.0	45.896507	42
TGTGTTA	3200	0.0	44.90049	42
>>END_MODULE