FastQCFastQC Report
Tue 24 May 2016
ERR841173.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841173.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5734513
Sequences flagged as poor quality0
Sequence length24-50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCC540510.9425560636099352No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATC240910.4201054213322038No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAAT201180.35082316493135507No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCCG197450.34431868931154225No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA172710.30117640329701933No Hit
AAGCAGTGGTATCAACGCAGAGTGCAG163840.28570865564346964No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGA146680.2557845801378426No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATC135120.2356259371981544No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG125480.21881544256678814No Hit
AAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTCTTCTGCT122310.21328751020356918Illumina Single End Adapter 2 (100% over 32bp)
ATTCCTGCTCACCGCAGTGTAGACAGGGAAACTTTCTTTGCAGTTCAGG119210.2078816457474244No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCC108290.18883905224384354No Hit
AAGCAGTGGTATCAACGCAGAGCG101820.17755648997569629No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGA100950.17603936027348793No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG98800.17229013169906493No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTAG93000.16217593368434252No Hit
AAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTCTTCTG91100.15886266192089896Illumina Single End Adapter 2 (100% over 30bp)
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTA90860.1584441433823587No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTG90620.15802562484381846No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGAC88970.15514830989135434No Hit
ACTACTTCTCCCCCGGACTCCTTGGTAGTCTGTTAGTGGGAGATCCTTG83770.1460804082229825No Hit
CTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAATCCT83350.14534800078053708No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGC73670.1284677530594141No Hit
AAGCAGTGGTATCAACGCAGAGTG69720.12157963544593936No Hit
CTCTTTCCCTTCGGTGTGCCACTGAAGATCCTGGTGTCGCCATGGGCCG67950.1184930612242051No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGT67290.11734213524321943No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAA62500.10898920274485382No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA62480.10895432619997548No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGT59370.10353102347139156No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCGCA2550.0128.8595744
CTATACG1700.096.6446844
ATGTTCT11900.083.9003244
CTATCAT9000.075.828944
TCGTGCG26950.071.3929243
AATCCCG32550.068.1452643
ACGACAC3050.066.2984944
CGCTACC8050.065.9381744
CGTGCGC15950.064.181244
ATCCTTG21200.061.8367343
ACTTCTA9750.057.033744
AACGACA6050.056.02478443
TGTGTTA34750.055.63539542
TAATCCC103300.054.25920542
CCCATAC5950.053.1014744
TAAGCCA11000.052.85044544
TAATCCT34750.052.34565442
TAATCGC2550.050.8229443
ACGCTAC14950.050.01228743
TTTGGAC21400.049.84557743
Produced by FastQC (version 0.11.5)