##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841173.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5734513
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.57240344559337	37.0	35.0	39.0	30.0	39.0
2	36.53121616430201	38.0	35.0	39.0	32.0	39.0
3	36.81386161300881	38.0	35.0	39.0	32.0	39.0
4	37.25176383766154	38.0	36.0	40.0	33.0	41.0
5	37.678252887385554	39.0	36.0	40.0	33.0	41.0
6	37.70591661401762	39.0	36.0	40.0	33.0	41.0
7	37.440711181577235	39.0	36.0	40.0	32.0	41.0
8	37.49697786019493	39.0	36.0	40.0	33.0	41.0
9	37.44735498899384	39.0	36.0	40.0	32.0	41.0
10	37.02603115556631	38.0	35.0	40.0	31.0	41.0
11	37.08156263661797	38.0	35.0	40.0	32.0	41.0
12	36.97753514553023	38.0	35.0	40.0	31.0	41.0
13	36.86528847349374	38.0	35.0	40.0	31.0	41.0
14	36.817691406401906	38.0	35.0	40.0	31.0	41.0
15	36.82503431416059	38.0	35.0	40.0	31.0	41.0
16	36.62607932007478	38.0	35.0	40.0	31.0	41.0
17	36.53830935599937	38.0	35.0	40.0	31.0	41.0
18	36.49257905597214	38.0	35.0	40.0	31.0	41.0
19	36.36979077386345	38.0	34.0	40.0	30.0	41.0
20	36.20551945736281	38.0	34.0	40.0	30.0	41.0
21	36.00500129653555	38.0	34.0	40.0	30.0	41.0
22	35.99417805836346	38.0	34.0	40.0	30.0	41.0
23	35.87166355713205	37.0	34.0	40.0	30.0	41.0
24	35.887991011616855	37.0	34.0	40.0	30.0	41.0
25	35.914211486022225	37.0	34.0	40.0	30.0	41.0
26	35.948691007573096	37.0	34.0	40.0	30.0	41.0
27	35.91222097124347	37.0	34.0	40.0	30.0	41.0
28	35.82271019297081	37.0	34.0	40.0	30.0	41.0
29	35.84974057819526	37.0	34.0	40.0	30.0	41.0
30	35.841277191525606	37.0	34.0	40.0	30.0	41.0
31	35.777970038382655	37.0	34.0	40.0	30.0	41.0
32	35.7569617498169	37.0	34.0	39.0	30.0	41.0
33	35.69812743045401	36.0	34.0	39.0	30.0	41.0
34	35.601943293294056	36.0	34.0	39.0	30.0	41.0
35	35.451031058889356	36.0	34.0	39.0	30.0	41.0
36	35.30524353417984	36.0	33.0	39.0	29.0	40.0
37	35.2653514799491	35.0	33.0	39.0	30.0	40.0
38	35.19643668447132	35.0	33.0	39.0	30.0	40.0
39	35.019836229455784	35.0	33.0	39.0	29.0	40.0
40	34.934265202808795	35.0	33.0	39.0	29.0	40.0
41	34.80255315052133	35.0	33.0	38.0	29.0	40.0
42	34.62579119690051	35.0	33.0	38.0	29.0	40.0
43	34.53848958569718	35.0	33.0	38.0	29.0	40.0
44	34.44450336230497	35.0	33.0	37.0	29.0	40.0
45	34.4230789484107	35.0	33.0	37.0	29.0	40.0
46	34.351263572816606	35.0	33.0	37.0	29.0	40.0
47	34.35278829780235	35.0	33.0	37.0	29.0	40.0
48	34.35362878896797	35.0	33.0	37.0	29.0	40.0
49	34.418485772308124	35.0	33.0	37.0	29.0	40.0
50	34.99576420394523	35.0	33.0	38.0	29.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	1.0
20	6.0
21	28.0
22	83.0
23	306.0
24	802.0
25	2408.0
26	6279.0
27	15673.0
28	36124.0
29	74529.0
30	138481.0
31	232537.0
32	351745.0
33	494017.0
34	641459.0
35	713826.0
36	817131.0
37	827038.0
38	698818.0
39	509688.0
40	173533.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	47.22653867904737	20.910598685537902	31.862862635414725
2	27.46569761024171	25.481239644935847	26.370085829433123	20.68297691538933
3	33.0719278167126	16.330994454106214	25.267742875463007	25.329334853718176
4	19.03605415141617	17.566583247784077	28.577021274517993	34.82034132628176
5	23.386833371900977	23.369133525375215	26.966684006122225	26.277349096601576
6	29.955324889838074	17.87843187381387	23.620715481855218	28.545527754492838
7	29.261368838121037	19.951441386565868	24.730086059618316	26.057103715694772
8	30.67699035646096	16.492612363072507	26.38408876220178	26.44630851826476
9	29.65953691272476	17.123250047562887	23.007097551265467	30.21011548844688
10	25.949753710559204	20.510268265151723	28.56409951464056	24.97587850964851
11	32.04409860087509	20.803056859405498	23.162629503150487	23.99021503656893
12	28.75115986309561	17.047219179728078	25.235987781351266	28.965633175825044
13	25.682302926159554	18.116795619784977	25.57941711876841	30.621484335287057
14	26.15449646726758	22.545157714351678	20.310198965457047	30.990146852923694
15	23.83266024508097	23.715911011100683	21.30365734631694	31.147771397501412
16	26.349438914865132	19.698446930018296	25.6284186643225	28.323695490794076
17	29.94346686457943	19.707968226770085	17.884866596343926	32.463698312306555
18	26.2544352066165	18.860590254133175	20.732013337488294	34.15296120176203
19	26.53867904737508	21.063915976823143	26.665647109004727	25.731757866797057
20	30.51913911434153	17.437993426817584	24.791067698338114	27.251799760502767
21	27.65980302076218	23.898018890183003	20.58275916368138	27.859418925373436
22	30.613375538602845	18.67119317717128	25.803586110974024	24.911845173251855
23	27.406703934579973	21.079383724476692	24.70684084245689	26.807071498486444
24	33.60236518776747	21.961324353088045	19.729748629046618	24.706561830097865
25	28.798191921276572	20.796710794841687	22.20464820238191	28.200449081499833
26	30.443764195080707	24.077042121327192	20.525531442327672	24.953662241264425
27	30.10059004564764	21.7220904205185	23.43999185394205	24.73732767989181
28	28.95868497067668	20.927858374682735	25.2211381848293	24.892318469811283
29	30.981252217925842	20.640052733888123	25.416421676289996	22.962273371896043
30	28.35404304393797	20.936069283850518	23.25811965876138	27.451768013450128
31	27.465214944970416	21.15941125689853	27.329709856451256	24.045663941679795
32	27.722035925569372	19.26485242387811	25.60397349264768	27.409138157904838
33	25.55452943928025	18.80706936795978	26.32389685605041	29.31450433670956
34	26.32428640752173	18.567954558183455	31.179092628472887	23.92866640582193
35	30.707852699145832	18.716553628061405	23.516626755752128	27.05896691704063
36	27.037573776242397	21.029958189958784	22.763707394582916	29.168760639215908
37	24.75387777630136	21.046870667440874	27.85411677429363	26.345134781964134
38	31.559959655951513	20.72169657555874	22.068603276370016	25.649740492119726
39	26.05430700069858	21.22647576923061	26.343974392964103	26.37524283710671
40	27.210232768475134	20.257891893928925	22.97233074143396	29.55954459616198
41	26.405952586952758	21.16630871071391	23.48538827193795	28.94235043039538
42	25.54273081364313	21.40751297637089	28.380730796013793	24.669025413972193
43	24.478390234365456	24.70187181644573	24.1724940911138	26.647243858075015
44	23.072699323995074	24.57049765657532	24.92757090077878	27.429232118650827
45	25.360284374864978	20.17378714012069	28.780150468797256	25.68577801621707
46	24.82939004061484	21.951354288503513	23.762237408017977	29.45701826286367
47	25.73198148898172	21.587190483421363	24.59046535963474	28.090362667962175
48	24.362727906547264	22.43685028324207	24.856183291334784	28.344238518875887
49	27.352761480675603	21.97311307213699	22.692961116048433	27.981164331138974
50	24.07043075853577	24.33350674842166	25.063213207414293	26.53284928562828
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3763.0
1	3704.0
2	3265.0
3	2836.5
4	2670.0
5	2182.0
6	1589.0
7	1474.5
8	1681.0
9	1841.0
10	1944.5
11	2019.0
12	2040.0
13	2145.5
14	2437.0
15	2737.0
16	2956.5
17	3349.5
18	3849.5
19	4526.0
20	5340.5
21	6775.0
22	8572.0
23	10859.5
24	13399.0
25	16022.5
26	19434.5
27	23414.0
28	27934.5
29	32915.0
30	38167.5
31	45014.5
32	54135.0
33	62246.5
34	71651.0
35	84542.5
36	96012.0
37	105194.5
38	114334.5
39	128832.5
40	147746.0
41	177525.0
42	217329.5
43	241898.5
44	252887.0
45	261763.5
46	280110.0
47	302264.5
48	346739.5
49	401729.0
50	460634.0
51	532418.5
52	574955.0
53	576081.0
54	538841.5
55	505408.0
56	501742.5
57	521863.0
58	528124.5
59	471540.0
60	408520.5
61	383449.5
62	365025.5
63	343745.5
64	323419.5
65	306959.0
66	281904.0
67	256040.5
68	229926.5
69	200998.0
70	176575.0
71	155212.0
72	140474.0
73	128311.0
74	111740.0
75	94639.0
76	82431.0
77	66183.0
78	51556.0
79	42251.0
80	33265.0
81	24638.5
82	18342.0
83	13895.5
84	10201.0
85	7575.5
86	5603.0
87	3960.5
88	2764.0
89	2082.0
90	1442.5
91	849.0
92	562.0
93	451.0
94	302.0
95	146.0
96	100.5
97	98.0
98	56.0
99	14.0
100	14.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	84759.0
25	66834.0
26	63686.0
27	69135.0
28	70636.0
29	51930.0
30	65000.0
31	52205.0
32	53884.0
33	49958.0
34	52313.0
35	59679.0
36	50101.0
37	51478.0
38	57369.0
39	59126.0
40	60756.0
41	64325.0
42	68411.0
43	68168.0
44	71048.0
45	100472.0
46	345837.0
47	672685.0
48	1070909.0
49	1373455.0
50	880354.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.965833987314355
#Duplication Level	Percentage of deduplicated	Percentage of total
1	57.94554567116388	12.728222365171277
2	13.06117401644094	5.737991602491311
3	7.096031580003184	4.6761075496526985
4	4.614220049374951	4.054207663420304
5	3.4451681294683447	3.783799559514399
6	2.456736724788589	3.237856262834672
7	1.9415818278702694	2.9853924872649285
8	1.5229634384226223	2.6762529645712614
9	1.1937256367275588	2.3599061246482815
>10	6.116115159380618	22.880502398016606
>50	0.32502736239297336	4.940759060526053
>100	0.23175024790523463	9.899933941801773
>500	0.027320630804997834	4.082819375071677
>1k	0.019575529356563936	8.386000562932963
>5k	0.0018724419384539364	2.9404218256572854
>10k+	0.001191553960834323	4.629826256424642
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCC	54051	0.9425560636099352	No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATC	24091	0.4201054213322038	No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAAT	20118	0.35082316493135507	No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCCG	19745	0.34431868931154225	No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA	17271	0.30117640329701933	No Hit
AAGCAGTGGTATCAACGCAGAGTGCAG	16384	0.28570865564346964	No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGA	14668	0.2557845801378426	No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATC	13512	0.2356259371981544	No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG	12548	0.21881544256678814	No Hit
AAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTCTTCTGCT	12231	0.21328751020356918	Illumina Single End Adapter 2 (100% over 32bp)
ATTCCTGCTCACCGCAGTGTAGACAGGGAAACTTTCTTTGCAGTTCAGG	11921	0.2078816457474244	No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCC	10829	0.18883905224384354	No Hit
AAGCAGTGGTATCAACGCAGAGCG	10182	0.17755648997569629	No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGA	10095	0.17603936027348793	No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG	9880	0.17229013169906493	No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTAG	9300	0.16217593368434252	No Hit
AAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTCTTCTG	9110	0.15886266192089896	Illumina Single End Adapter 2 (100% over 30bp)
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTA	9086	0.1584441433823587	No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTG	9062	0.15802562484381846	No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGAC	8897	0.15514830989135434	No Hit
ACTACTTCTCCCCCGGACTCCTTGGTAGTCTGTTAGTGGGAGATCCTTG	8377	0.1460804082229825	No Hit
CTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAATCCT	8335	0.14534800078053708	No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGC	7367	0.1284677530594141	No Hit
AAGCAGTGGTATCAACGCAGAGTG	6972	0.12157963544593936	No Hit
CTCTTTCCCTTCGGTGTGCCACTGAAGATCCTGGTGTCGCCATGGGCCG	6795	0.1184930612242051	No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGT	6729	0.11734213524321943	No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAA	6250	0.10898920274485382	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA	6248	0.10895432619997548	No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGT	5937	0.10353102347139156	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.13333303106994437	0.0	0.0	0.0	0.0
2	0.516486753103533	0.0	0.0	0.0	0.0
3	0.6910438602196909	0.0	0.0	0.0	0.0
4	0.8189884651059297	0.0	0.0	0.0	0.0
5	1.1225539117271162	0.0	0.0	0.0	0.0
6	1.3008776856901363	0.0	0.0	0.0	0.0
7	1.45074219903242	0.0	0.0	0.0	0.0
8	1.567142667563924	1.7438272439176614E-5	0.0	0.0	0.0
9	1.667674308175777	1.7438272439176614E-5	0.0	0.0	0.0
10	1.7507676763484536	1.7438272439176614E-5	0.0	0.0	3.487654487835323E-5
11	1.8473931439339313	1.7438272439176614E-5	0.0	0.0	3.487654487835323E-5
12	1.9098570358110618	1.7438272439176614E-5	0.0	0.0	3.487654487835323E-5
13	1.95648697631342	1.7438272439176614E-5	0.0	0.0	3.487654487835323E-5
14	2.0140855901800205	3.3132717634435564E-4	0.0	0.0	3.487654487835323E-5
15	2.113448866538449	3.3132717634435564E-4	0.0	0.0	3.487654487835323E-5
16	2.151080658462192	3.3132717634435564E-4	0.0	0.0	3.487654487835323E-5
17	2.220275723500845	3.3132717634435564E-4	0.0	0.0	3.487654487835323E-5
18	2.2752585965015686	3.3132717634435564E-4	0.0	0.0	3.487654487835323E-5
19	2.842438407585788	3.3132717634435564E-4	0.0	0.0	3.487654487835323E-5
20	2.8779601685443907	3.3132717634435564E-4	0.0	0.0	3.487654487835323E-5
21	3.053371751010068	3.3132717634435564E-4	0.0	0.0	3.487654487835323E-5
22	3.0711762271704677	3.3132717634435564E-4	0.0	0.0	3.487654487835323E-5
23	3.089870055225265	3.3132717634435564E-4	0.0	0.0	3.487654487835323E-5
24	3.10882545736665	3.3132717634435564E-4	0.0	0.0	3.487654487835323E-5
25	3.131024378181722	3.3132717634435564E-4	0.0	0.0	3.487654487835323E-5
26	3.3856754706110177	3.3132717634435564E-4	0.0	0.0	3.487654487835323E-5
27	3.4040554097619102	3.3132717634435564E-4	0.0	0.0	3.487654487835323E-5
28	3.420621768579128	3.3132717634435564E-4	0.0	0.0	3.487654487835323E-5
29	3.4366649792231705	3.3132717634435564E-4	0.0	0.0	3.487654487835323E-5
30	3.449081029199864	3.3132717634435564E-4	0.0	0.0	3.487654487835323E-5
31	3.462107418711929	3.3132717634435564E-4	0.0	0.0	3.487654487835323E-5
32	3.4733376661627586	3.3132717634435564E-4	0.0	0.0	3.487654487835323E-5
33	3.485300321056034	3.3132717634435564E-4	0.0	0.0	3.487654487835323E-5
34	3.4963910623273504	5.40586445614475E-4	0.0	0.0	3.487654487835323E-5
35	3.508301402403308	5.40586445614475E-4	0.0	0.0	3.487654487835323E-5
36	3.519566526399016	5.40586445614475E-4	0.0	0.0	3.487654487835323E-5
37	3.5280415268044556	5.40586445614475E-4	0.0	0.0	3.487654487835323E-5
38	3.5347901382384173	5.40586445614475E-4	0.0	0.0	3.487654487835323E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATCGCA	255	0.0	128.85957	44
CTATACG	170	0.0	96.64468	44
ATGTTCT	1190	0.0	83.90032	44
CTATCAT	900	0.0	75.8289	44
TCGTGCG	2695	0.0	71.39292	43
AATCCCG	3255	0.0	68.14526	43
ACGACAC	305	0.0	66.29849	44
CGCTACC	805	0.0	65.93817	44
CGTGCGC	1595	0.0	64.1812	44
ATCCTTG	2120	0.0	61.83673	43
ACTTCTA	975	0.0	57.0337	44
AACGACA	605	0.0	56.024784	43
TGTGTTA	3475	0.0	55.635395	42
TAATCCC	10330	0.0	54.259205	42
CCCATAC	595	0.0	53.10147	44
TAAGCCA	1100	0.0	52.850445	44
TAATCCT	3475	0.0	52.345654	42
TAATCGC	255	0.0	50.82294	43
ACGCTAC	1495	0.0	50.012287	43
TTTGGAC	2140	0.0	49.845577	43
>>END_MODULE