FastQCFastQC Report
Tue 24 May 2016
ERR841172.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841172.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4729741
Sequences flagged as poor quality0
Sequence length24-50
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG348200.7361925314726536No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCGC248030.5244050361319996No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGT222910.4712943055444262No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCC215570.45577548538070056No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCCG210340.44471779744387696No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG175250.37052768851402224No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG167950.35509343957734685No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA143560.30352613388344096No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTG111480.23570000978911954No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA109340.23117544914192978No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAAG106290.2247268930793462No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGC106040.224198322910282No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG101960.2155720577511538No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCC101870.2153817724902907No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATC100300.21206235182856734No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA95010.20087780705116834No Hit
ATCCGAATTCAGTCTTCCTGAGGCTCTGGCTCACCTCCAGGCAGGTCCC89230.18865726474240346No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGAC89120.1884246938680152No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAAT88770.18768469563132528No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCG85420.18060185536586465No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTGC83820.1772190062838536No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTC79080.1671973158783959No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGA78210.16535789169005238No Hit
ATCCGAATTCAGTCTTCCTGAGGCTCTGGCTCACCTCCAGGCAGGTCCCA77900.16470246468041275No Hit
CTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAATCCTG74220.15692191179178733No Hit
ATTCCTGCTCACCGCAGTGTAGACAGGGAAACTTTCTTTGCAGTTCAGG73050.15444820340056675No Hit
AAACAGCCGGGGCTCCAGCGGGAGAACGATAATGCAAAGTGCTATGTTCT70870.1498390715263267No Hit
ATTCCTGCTCACCGCAGTGTAGACAGGGAAACTTTCTTTGCAGTTCAGGA68470.14476479790331015No Hit
AGGCCTGGTTTGCGCTGGAGAGTGGTCTTGGTTGTGAGGGTCATAAGAT64780.13696310220792218No Hit
CTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAATCCT63270.13377053838677425No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATG56140.11869571716506254No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGG53720.11357915792852083No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTG52350.11068259340204886No Hit
AGGCCTGGTTTGCGCTGGAGAGTGGTCTTGGTTGTGAGGGTCATAAGATG51840.10960431025715785No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGGTTGTGAGGGTTCCTGCTT51690.10928716815571932No Hit
CTCTGCAGGTACCATGGAGCTGAGCTATAGGCTCTTCATCTGCCTCCTG51380.10863174114607967No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGGTTGTGAGGG48990.10357861032982568No Hit
CGGCTCCTGGCCAGGTAACTTACTACTTCCAGTCAATATGCCGACCTT48510.10256375560522236No Hit
CGGCTCCTGGCCAGGTAACTTACTACTTCCAGTCAATATGCCGACCTTA47600.10063976018982858No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCGC41850.0139.2723144
TAAGCCA18050.0118.5964544
ATGTTCT15300.0112.8999444
CCAGAAT21350.0105.2290244
CTATACG1400.098.4040244
AATGAAG25500.086.0255944
TCGTGCG76100.078.4897443
GGTCCCA21550.072.2882244
CGCTACC6200.071.7885444
CAATGAA34500.071.7148343
AATCCCG29900.071.3237443
TTCAGGA27150.070.6490444
TTAAGCC13650.070.0004843
ATTAGTC5600.066.23347544
AGGTCCC26050.064.14519543
GACCTTA6950.062.43186643
GACCGTA855.6522567E-662.33738744
ACGACAC2900.062.1224344
AATCCTG16150.057.16375743
TCCTTGT16950.056.8943644
Produced by FastQC (version 0.11.5)