##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841172.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4729741
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.95033998690415	35.0	32.0	37.0	22.0	39.0
2	33.90762158012458	35.0	33.0	37.0	27.0	39.0
3	34.50573297776771	35.0	33.0	37.0	30.0	39.0
4	34.78022369512411	35.0	33.0	38.0	31.0	39.0
5	35.29461338369268	35.0	34.0	38.0	31.0	41.0
6	35.392641372963126	35.0	34.0	38.0	32.0	41.0
7	35.34478991555775	35.0	34.0	38.0	31.0	41.0
8	35.147589899742925	35.0	34.0	38.0	31.0	41.0
9	35.07189505725578	35.0	34.0	38.0	31.0	40.0
10	34.91262777390982	35.0	34.0	38.0	31.0	40.0
11	34.673533286494965	35.0	33.0	38.0	30.0	40.0
12	34.62433164944973	35.0	33.0	38.0	30.0	40.0
13	34.41127664284366	35.0	33.0	37.0	29.0	40.0
14	34.43530184845217	35.0	33.0	37.0	30.0	40.0
15	34.37169646287186	35.0	33.0	36.0	30.0	40.0
16	34.322813447924524	35.0	33.0	36.0	30.0	40.0
17	34.26751887682645	35.0	33.0	36.0	30.0	39.0
18	34.13195648556655	35.0	33.0	36.0	30.0	39.0
19	33.99641883139056	35.0	33.0	35.0	29.0	39.0
20	33.86112199378359	35.0	33.0	35.0	29.0	39.0
21	33.611149955145535	35.0	33.0	35.0	29.0	39.0
22	33.581475391570066	35.0	33.0	35.0	29.0	38.0
23	33.572076779679904	35.0	33.0	35.0	29.0	38.0
24	33.499612135209944	35.0	33.0	35.0	29.0	37.0
25	33.468131981797825	35.0	33.0	35.0	29.0	37.0
26	33.453131416250066	35.0	33.0	35.0	29.0	37.0
27	33.465481738329224	35.0	33.0	35.0	29.0	37.0
28	33.509982354229756	35.0	33.0	35.0	29.0	37.0
29	33.53599796821202	35.0	33.0	35.0	30.0	37.0
30	33.57040585236606	35.0	33.0	35.0	30.0	36.0
31	33.5029547153213	35.0	33.0	35.0	30.0	36.0
32	33.52847079478541	35.0	33.0	35.0	30.0	36.0
33	33.48875880689431	35.0	33.0	35.0	30.0	36.0
34	33.500747391098756	35.0	33.0	35.0	30.0	36.0
35	33.47383359506768	35.0	33.0	35.0	30.0	36.0
36	33.410933647562125	35.0	33.0	35.0	29.0	35.0
37	33.41169269362359	35.0	33.0	35.0	30.0	35.0
38	33.42291347039338	35.0	33.0	35.0	30.0	35.0
39	33.35276267021069	35.0	33.0	35.0	29.0	35.0
40	33.23402426468836	35.0	33.0	35.0	29.0	35.0
41	33.18884221665247	35.0	33.0	35.0	29.0	35.0
42	33.159920266928964	34.0	33.0	35.0	29.0	35.0
43	33.145880858756094	34.0	33.0	35.0	29.0	35.0
44	33.12630155266622	34.0	33.0	35.0	29.0	35.0
45	33.14655818758936	34.0	33.0	35.0	29.0	35.0
46	33.08555851856583	34.0	33.0	35.0	29.0	35.0
47	33.063680264862846	34.0	33.0	35.0	29.0	35.0
48	33.07409165848743	34.0	33.0	35.0	29.0	35.0
49	33.05745260671649	34.0	33.0	35.0	29.0	35.0
50	33.083261328833615	34.0	33.0	35.0	29.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	20.0
21	123.0
22	535.0
23	1725.0
24	4840.0
25	12009.0
26	26405.0
27	52155.0
28	96088.0
29	161853.0
30	255632.0
31	378803.0
32	543457.0
33	800822.0
34	1214133.0
35	397801.0
36	374830.0
37	246678.0
38	93806.0
39	53924.0
40	14101.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	51.74207213460525	15.313608081288171	32.944319784106575
2	30.115560238922175	19.580332200008417	26.890774780268096	23.413332780801316
3	30.046613546069434	16.07018227847994	24.248177648628115	29.635026526822507
4	20.515330543469503	17.186120762215097	30.065113501986684	32.23343519232871
5	24.0905368814064	18.189304657485472	27.179754663098887	30.540403798009237
6	29.11814832989798	18.756206735210238	24.93417715684643	27.191467778045354
7	33.61446218725296	20.225906661696698	20.567362990912187	25.592268160138154
8	28.96871097170014	16.673640269097188	28.061790275619742	26.29585848358293
9	30.16317383975148	17.86157000985889	23.883041375838552	28.092214774551078
10	31.642303458054045	18.63820873066834	25.512517493029748	24.20697031824787
11	35.403756780762414	16.517669783609715	25.037798052789782	23.04077538283809
12	34.38114264607724	16.0077898557236	22.406639179608355	27.204428318590807
13	29.005943454409028	16.867625521143758	27.8754164340077	26.25101459043952
14	27.966880216062574	18.67780920773463	22.043469187847705	31.311841388355095
15	26.574837818815027	18.91951377464432	24.31198663943755	30.1936617671031
16	27.46264541758206	22.891951166036364	25.6257372232433	24.01966619313827
17	28.348000450764637	21.08083719594794	18.1356864995356	32.435475853751825
18	27.004692223104815	22.145102659955377	22.64379804306409	28.206407073875717
19	30.818854563072268	17.510705131634058	25.248803264280223	26.42163704101345
20	29.49032515733948	18.23780625619881	24.158447576727774	28.113421009733937
21	32.464441499016544	20.21812610880807	21.29169440779104	26.025737984384346
22	30.337475138702096	20.48310890596335	22.03097378905103	27.148442166283527
23	30.554104336791383	21.97545277849252	20.97531344739596	26.49512943732014
24	33.52663496796125	21.461196289606555	19.44706908898394	25.565099653448254
25	29.33020261278533	23.57800582618346	22.697389159434874	24.394402401596334
26	31.610510223985656	19.75459237445184	22.80391302553982	25.830984376022688
27	29.138182113256473	21.324936370561097	22.899857689935075	26.63702382624735
28	30.671695105748032	21.291630753176076	23.712766929426827	24.32390721164907
29	33.817636816566534	19.905705678905125	22.598468566450588	23.678188938077753
30	28.78828363118815	23.731577086438318	20.85831867556427	26.62182060680926
31	28.78345694116689	19.5991329548397	26.72177099740723	24.895639106586174
32	27.940945442937682	19.789410081456527	25.90297039861826	26.366674076987533
33	26.696043700428845	18.89141563200143	27.219024273002447	27.19351639456728
34	26.065810364256777	18.06178123411968	28.550061268418848	27.322347133204694
35	29.83902751983159	18.75060630046353	24.82907599106401	26.58129018864087
36	27.3849681275466	19.451542986403926	23.56663874573496	29.596850140314512
37	26.139474325390776	19.88462850047835	26.529288052418075	27.446609121712807
38	28.7361556336167	20.60803768758223	21.686630988755056	28.96917569004602
39	29.625436270228178	20.160112540407766	25.42188682545911	24.792564363904948
40	27.673718134694152	19.82423614282496	23.572201167910396	28.929844554570494
41	26.91351339266862	22.836294715714743	22.75772472433434	27.492467167282292
42	26.780455752465787	21.090808894818856	25.248261616101743	26.880473736613613
43	24.673673598829737	24.16715113987263	25.435860579546937	25.723314681750697
44	24.199386352921778	22.92144441496145	22.889860396577795	29.989308835538974
45	27.535256091574627	19.339146129156596	26.656081814435744	26.46951596483303
46	24.731492718446603	20.376586186339633	24.810030233350368	30.081890861863396
47	31.331097723391476	18.608291326239694	22.687607127744766	27.373003822624064
48	23.62668753587898	22.073864231711468	24.17203083522312	30.127417397186434
49	29.467818902731736	21.66160935427725	20.627895356336822	28.242676386654193
50	25.962334483897383	23.368023668746126	22.55486290494049	28.114778942416002
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1442.0
1	1395.5
2	1046.5
3	731.0
4	711.0
5	641.5
6	535.5
7	526.5
8	585.0
9	636.0
10	694.0
11	740.5
12	761.0
13	782.0
14	806.0
15	883.5
16	1028.5
17	1214.0
18	1416.0
19	1773.5
20	2253.0
21	2977.0
22	3919.5
23	5149.0
24	6505.0
25	7966.0
26	9842.5
27	11885.5
28	14060.0
29	16658.0
30	19747.0
31	24202.5
32	30453.5
33	36049.5
34	42731.5
35	52311.0
36	61048.0
37	68397.5
38	75494.0
39	86283.5
40	100076.5
41	118413.0
42	148583.5
43	188139.0
44	213836.5
45	214584.0
46	217784.5
47	235622.5
48	264625.5
49	304972.0
50	348760.5
51	379591.0
52	399190.5
53	413155.0
54	415145.5
55	406922.0
56	397380.0
57	410655.5
58	431483.5
59	418024.5
60	396639.5
61	392168.5
62	385847.5
63	369020.0
64	335511.0
65	297372.5
66	258352.5
67	222744.0
68	198899.5
69	177444.5
70	155469.5
71	138063.5
72	125280.0
73	110414.5
74	90866.0
75	73801.0
76	62773.5
77	49733.5
78	38352.5
79	30176.0
80	22687.5
81	16706.5
82	12222.5
83	8925.0
84	6467.0
85	4660.0
86	3252.0
87	2188.5
88	1433.0
89	999.0
90	645.5
91	376.0
92	266.0
93	217.5
94	153.5
95	81.0
96	59.5
97	59.0
98	33.5
99	7.0
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	50520.0
25	49862.0
26	45003.0
27	42191.0
28	37758.0
29	35787.0
30	46705.0
31	36140.0
32	42019.0
33	38112.0
34	37958.0
35	41466.0
36	35757.0
37	35885.0
38	38723.0
39	45166.0
40	44979.0
41	44426.0
42	49178.0
43	50396.0
44	49819.0
45	74451.0
46	262197.0
47	584416.0
48	873513.0
49	1153110.0
50	884204.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.48953814919249
#Duplication Level	Percentage of deduplicated	Percentage of total
1	55.62192618511084	10.840456523162866
2	13.695830250755073	5.338508123139111
3	7.391075953861763	4.321459702991044
4	4.8656150326603065	3.7931435919327003
5	3.470301294905962	3.381728473813092
6	2.61731096445363	3.0606109134011295
7	1.9881551941431894	2.712375855193823
8	1.5305546745176983	2.3863842974750065
9	1.2192772643042982	2.1386835681391507
>10	6.837575244894064	23.19130324262997
>50	0.4149169734470305	5.595310487664127
>100	0.29151346316202126	11.268702683353863
>500	0.028983420220068338	3.86347990654829
>1k	0.022687841236916848	8.923033093053371
>5k	0.0024944746909699154	3.468739840607519
>10k+	0.0017817676364070823	5.716079696894976
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG	34820	0.7361925314726536	No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCGC	24803	0.5244050361319996	No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGT	22291	0.4712943055444262	No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCC	21557	0.45577548538070056	No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCCG	21034	0.44471779744387696	No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG	17525	0.37052768851402224	No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG	16795	0.35509343957734685	No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA	14356	0.30352613388344096	No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTG	11148	0.23570000978911954	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA	10934	0.23117544914192978	No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAAG	10629	0.2247268930793462	No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGC	10604	0.224198322910282	No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG	10196	0.2155720577511538	No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCC	10187	0.2153817724902907	No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATC	10030	0.21206235182856734	No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA	9501	0.20087780705116834	No Hit
ATCCGAATTCAGTCTTCCTGAGGCTCTGGCTCACCTCCAGGCAGGTCCC	8923	0.18865726474240346	No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGAC	8912	0.1884246938680152	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAAT	8877	0.18768469563132528	No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCG	8542	0.18060185536586465	No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTGC	8382	0.1772190062838536	No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTC	7908	0.1671973158783959	No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGA	7821	0.16535789169005238	No Hit
ATCCGAATTCAGTCTTCCTGAGGCTCTGGCTCACCTCCAGGCAGGTCCCA	7790	0.16470246468041275	No Hit
CTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAATCCTG	7422	0.15692191179178733	No Hit
ATTCCTGCTCACCGCAGTGTAGACAGGGAAACTTTCTTTGCAGTTCAGG	7305	0.15444820340056675	No Hit
AAACAGCCGGGGCTCCAGCGGGAGAACGATAATGCAAAGTGCTATGTTCT	7087	0.1498390715263267	No Hit
ATTCCTGCTCACCGCAGTGTAGACAGGGAAACTTTCTTTGCAGTTCAGGA	6847	0.14476479790331015	No Hit
AGGCCTGGTTTGCGCTGGAGAGTGGTCTTGGTTGTGAGGGTCATAAGAT	6478	0.13696310220792218	No Hit
CTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAATCCT	6327	0.13377053838677425	No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATG	5614	0.11869571716506254	No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGG	5372	0.11357915792852083	No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTG	5235	0.11068259340204886	No Hit
AGGCCTGGTTTGCGCTGGAGAGTGGTCTTGGTTGTGAGGGTCATAAGATG	5184	0.10960431025715785	No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGGTTGTGAGGGTTCCTGCTT	5169	0.10928716815571932	No Hit
CTCTGCAGGTACCATGGAGCTGAGCTATAGGCTCTTCATCTGCCTCCTG	5138	0.10863174114607967	No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGGTTGTGAGGG	4899	0.10357861032982568	No Hit
CGGCTCCTGGCCAGGTAACTTACTACTTCCAGTCAATATGCCGACCTT	4851	0.10256375560522236	No Hit
CGGCTCCTGGCCAGGTAACTTACTACTTCCAGTCAATATGCCGACCTTA	4760	0.10063976018982858	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.10038604650867775	0.0	0.0	0.0	0.0
2	0.20032809407534155	0.0	0.0	0.0	0.0
3	0.27663248368145316	0.0	0.0	0.0	0.0
4	0.33344320545247613	0.0	0.0	0.0	0.0
5	0.3956030573344291	0.0	0.0	0.0	0.0
6	0.46323889616788744	0.0	0.0	0.0	0.0
7	0.5208953302094131	0.0	0.0	0.0	0.0
8	0.5620180893626099	0.0	0.0	0.0	0.0
9	0.6000539987284716	0.0	0.0	0.0	0.0
10	0.6342842028770709	0.0	0.0	0.0	0.0
11	0.6623618502577625	0.0	0.0	0.0	0.0
12	0.6876697899525577	0.0	0.0	0.0	0.0
13	0.7075017426958474	0.0	0.0	0.0	0.0
14	0.7269742677241735	0.0	0.0	0.0	0.0
15	0.7446073685641561	0.0	0.0	0.0	0.0
16	0.7616273280080241	0.0	0.0	0.0	0.0
17	0.7768712916838364	0.0	0.0	0.0	0.0
18	0.7945678209441067	0.0	0.0	0.0	0.0
19	0.8097694990063938	0.0	0.0	0.0	0.0
20	0.8227300395518486	0.0	0.0	0.0	0.0
21	0.8369802913098201	0.0	0.0	0.0	0.0
22	0.8481859788939817	0.0	0.0	0.0	0.0
23	0.8614425187341125	0.0	0.0	0.0	0.0
24	0.8723733498303607	0.0	0.0	0.0	0.0
25	0.8858201749313546	0.0	0.0	0.0	0.0
26	0.897279576196667	0.0	0.0	0.0	0.0
27	0.9072801237953622	0.0	0.0	0.0	0.0
28	0.9183800973457109	0.0	0.0	0.0	0.0
29	0.9286343586255569	0.0	0.0	0.0	0.0
30	0.9411086146154726	0.0	0.0	0.0	0.0
31	0.9515108755426566	0.0	0.0	0.0	0.0
32	0.9612365666534383	0.0	0.0	0.0	0.0
33	0.9723788258173122	0.0	0.0	2.1142806762569027E-5	0.0
34	0.9814490899184543	0.0	0.0	2.1142806762569027E-5	0.0
35	0.9915130659374372	0.0	0.0	2.1142806762569027E-5	0.0
36	1.0011964714346937	0.0	0.0	2.1142806762569027E-5	0.0
37	1.00923073800447	0.0	0.0	2.1142806762569027E-5	0.0
38	1.0148970102168384	0.0	0.0	2.1142806762569027E-5	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTGCGC	4185	0.0	139.27231	44
TAAGCCA	1805	0.0	118.59645	44
ATGTTCT	1530	0.0	112.89994	44
CCAGAAT	2135	0.0	105.22902	44
CTATACG	140	0.0	98.40402	44
AATGAAG	2550	0.0	86.02559	44
TCGTGCG	7610	0.0	78.48974	43
GGTCCCA	2155	0.0	72.28822	44
CGCTACC	620	0.0	71.78854	44
CAATGAA	3450	0.0	71.71483	43
AATCCCG	2990	0.0	71.32374	43
TTCAGGA	2715	0.0	70.64904	44
TTAAGCC	1365	0.0	70.00048	43
ATTAGTC	560	0.0	66.233475	44
AGGTCCC	2605	0.0	64.145195	43
GACCTTA	695	0.0	62.431866	43
GACCGTA	85	5.6522567E-6	62.337387	44
ACGACAC	290	0.0	62.12243	44
AATCCTG	1615	0.0	57.163757	43
TCCTTGT	1695	0.0	56.89436	44
>>END_MODULE