FastQCFastQC Report
Tue 24 May 2016
ERR841171.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841171.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10488448
Sequences flagged as poor quality0
Sequence length24-50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA439380.41891803248678927No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCC389260.3711321255537521No Hit
ATCCGAATTCAGTCTTCCTGAGGCTCTGGCTCACCTCCAGGCAGGTCCC325560.3103986404852272No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTG295870.2820913065498346No Hit
ACTACTTCTCCCCCGGACTCCTTGGTAGTCTGTTAGTGGGAGATCCTTG263940.25164828962302144No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCCG255180.2432962436387157No Hit
ATTCCTGCTCACCGCAGTGTAGACAGGGAAACTTTCTTTGCAGTTCAGG251860.24013085634786005No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA238060.2269735236328578No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGA229180.21850706605972592No Hit
ACTACTTCTCCCCCGGACTCCTTGGTAGTCTGTTAGTGGGAGATCCTTGT213540.20359542231605668No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA212730.20282314409148045No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA207550.1978843771738202No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGAC200390.19105781904052915No Hit
ATCCGAATTCAGTCTTCCTGAGGCTCTGGCTCACCTCCAGGCAGGTCCCA196120.18698667333813354No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATC187820.1790732051109945No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGC185030.1764131356707875No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTAG178900.17056861034158724No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG176210.1680038838920687No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAAT165240.1575447578135488No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGT160850.15335920052232704No Hit
ATCCGAATTCAGTCTTCCTGAGGCTCTGGCTCACCTCCAGGCAGGTC154910.14769582687543475No Hit
ATTCCTGCTCACCGCAGTGTAGACAGGGAAACTTTCTTTGCAGTTCAGGA153010.14588431005235475No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTGC147580.1407071856579734No Hit
AGCGAGCGGCCTTGAGAGGCTCTGGCTCTTGCTTCTTAGGCGGCCCGAG146760.13992537313432835No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAAT140500.13395690191723314No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG139510.13301300630941776No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTG129370.12334522705361176No Hit
AAACAGCCGGGGCTCCAGCGGGAGAACGATAATGCAAAGTGCTATGTTCT128770.12277316910948122No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAAC128050.12208669957652458No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCC126570.12067562331433593No Hit
CTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAATCCT123500.11774859350020135No Hit
ACTACTTCTCCCCCGGACTCCTTGGTAGTCTGTTAGTGGGAGATCCT121380.1157273220976068No Hit
AGGCCTGGTTTGCGCTGGAGAGTGGTCTTGGTTGTGAGGGTCATAAGAT119450.11388720237732025No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGG118750.11321980144250131No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACC117290.11182779377845034No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAAG113980.10867194078666358No Hit
ATTCCTGCTCACCGCAGTGTAGACAGGGAAACTTTCTTTGCAGTTCA106480.10152121648503191No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGCCA65150.0149.6267744
CTATACG4850.0139.973744
ATTAGTC17250.0114.37524444
ATGTTCT28350.0109.6283244
CCAGAAT35600.092.06969544
TCCTTGT58500.086.6722944
GGTCCCA54050.084.78055644
CGTGCGC34150.077.9634844
TTAAGCC55400.077.50407443
AATCGCA2900.073.1543644
TTCAGGA53000.069.0480544
ATCCTTG68550.068.6306443
AGGTCCC78200.066.3016443
AATCCCG40950.065.84309443
TATTAGT13450.064.19047543
TCGTGCG46500.062.1545243
CAATGAA55700.061.33775343
AATGAAG45300.060.8812244
CTATGCG4300.056.7371644
ATTAAGC74700.056.6444442
Produced by FastQC (version 0.11.5)