##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841171.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10488448
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.005978291545134	35.0	32.0	37.0	23.0	39.0
2	33.817799068079474	35.0	33.0	37.0	25.0	39.0
3	34.49636514382299	35.0	33.0	37.0	30.0	39.0
4	34.81513690109347	35.0	34.0	37.0	31.0	39.0
5	35.33658802522547	35.0	34.0	38.0	32.0	41.0
6	35.43119706557157	35.0	34.0	38.0	32.0	41.0
7	35.382864175900956	35.0	34.0	38.0	32.0	41.0
8	35.28569098116328	35.0	34.0	38.0	31.0	41.0
9	35.23579036669677	35.0	34.0	38.0	31.0	41.0
10	35.13957460627159	35.0	34.0	38.0	31.0	41.0
11	35.056545544202535	35.0	34.0	38.0	31.0	40.0
12	34.96306136046058	35.0	34.0	38.0	31.0	40.0
13	34.86640997791093	35.0	34.0	38.0	30.0	40.0
14	34.83897350685249	35.0	33.0	38.0	30.0	40.0
15	34.78631767064107	35.0	33.0	38.0	30.0	40.0
16	34.7726552107614	35.0	33.0	38.0	30.0	40.0
17	34.74852695079387	35.0	33.0	38.0	30.0	40.0
18	34.66893624299801	35.0	33.0	38.0	30.0	40.0
19	34.59835382699137	35.0	33.0	38.0	30.0	40.0
20	34.53435932561233	35.0	33.0	38.0	30.0	40.0
21	34.34465280277883	35.0	33.0	37.0	29.0	40.0
22	34.38623683885356	35.0	33.0	37.0	30.0	40.0
23	34.4166049161897	35.0	33.0	37.0	30.0	40.0
24	34.401602887290856	35.0	33.0	37.0	30.0	40.0
25	34.398603116329625	35.0	33.0	37.0	30.0	40.0
26	34.377301289626246	35.0	33.0	37.0	30.0	40.0
27	34.440682458490166	35.0	33.0	37.0	30.0	40.0
28	34.48497966959186	35.0	33.0	37.0	30.0	40.0
29	34.51680687165394	35.0	33.0	37.0	30.0	40.0
30	34.56174814334551	35.0	33.0	37.0	30.0	40.0
31	34.5358614393069	35.0	33.0	37.0	30.0	40.0
32	34.54709123869939	35.0	33.0	36.0	30.0	40.0
33	34.51420269413356	35.0	33.0	36.0	30.0	40.0
34	34.518619871651254	35.0	33.0	36.0	30.0	40.0
35	34.4829500786679	35.0	33.0	36.0	30.0	40.0
36	34.42000149431209	35.0	33.0	36.0	30.0	40.0
37	34.381104986581214	35.0	33.0	36.0	30.0	40.0
38	34.368060156089804	35.0	33.0	36.0	30.0	40.0
39	34.2850751888554	35.0	33.0	35.0	30.0	40.0
40	34.193722298850474	35.0	33.0	35.0	30.0	39.0
41	34.06790263201957	35.0	33.0	35.0	29.0	39.0
42	34.014173751489054	35.0	33.0	35.0	29.0	39.0
43	33.93736681101367	35.0	33.0	35.0	29.0	39.0
44	33.874436704127675	35.0	33.0	35.0	29.0	39.0
45	33.86447632921797	35.0	33.0	35.0	29.0	39.0
46	33.80697263667946	35.0	33.0	35.0	29.0	38.0
47	33.805273651018936	35.0	33.0	35.0	29.0	38.0
48	33.89539866736313	35.0	33.0	35.0	29.0	39.0
49	33.87535341684412	35.0	33.0	35.0	29.0	39.0
50	34.475501619933574	35.0	33.0	37.0	29.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	5.0
20	60.0
21	283.0
22	1279.0
23	3976.0
24	10990.0
25	25928.0
26	55270.0
27	106190.0
28	189513.0
29	316125.0
30	490894.0
31	728256.0
32	1056935.0
33	1593348.0
34	2638055.0
35	594788.0
36	562623.0
37	647151.0
38	640542.0
39	572960.0
40	253277.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	47.916927270841214	19.000466036538484	33.082606692620296
2	28.626847365787576	20.833225277943885	28.12376054112105	22.416166815147484
3	29.128017796341272	15.74005992116279	26.384284881805204	28.74763740069074
4	20.70118477013949	17.84635820285327	29.361941823995313	32.09051520301192
5	24.119412138001735	18.637161570520252	28.129910164020455	29.11351612745756
6	27.37839764281617	18.91735555155539	25.536342459818652	28.16790434580979
7	31.816118075810646	19.14157366275735	22.42978179421779	26.612526467214217
8	27.321182314104046	16.40146378186744	27.753248145006776	28.524105759021733
9	26.42588302864256	17.866265819309017	26.33677546954516	29.371075682503268
10	28.20688056040322	18.08024409331104	26.27530784344834	27.437567502837407
11	31.878701214898523	18.122957753139453	24.20365720457402	25.794683827388
12	30.271161186097313	16.717640207588385	23.836090906872016	29.175107699442282
13	26.96747888724814	17.309968071539277	27.08623811644964	28.63631492476294
14	27.403768412638364	18.481752495698125	21.900304029728705	32.21417506193481
15	26.061148417764002	19.396749643035843	24.7826179812304	29.759483957969756
16	29.294496192382326	20.162754298824765	25.59474004161531	24.948009467177602
17	29.749968727499056	20.448506776217034	18.37782863584774	31.42369586043617
18	27.64304118207003	20.32542850953735	21.1818469233961	30.849683384996524
19	29.033828455840176	18.214839793265885	26.418093506303315	26.333238244590618
20	30.3564168883709	17.750719648893718	24.02162836675169	27.871235095983693
21	29.483122765160296	21.067673692046718	22.152524377295858	27.296679165497128
22	30.742927838322696	20.242651725021663	23.98316700430798	25.03125343234767
23	30.018006477221416	21.17750881731978	22.694797171135328	26.109687534323477
24	32.67906748453156	21.065547543354366	20.061576317106212	26.19380865500787
25	30.44135046922572	21.207819751203395	23.5985913451086	24.75223843446228
26	30.62728282413171	20.697839016504894	23.329972402479076	25.344905756884323
27	29.42699604996549	21.459166691299348	23.707533053538814	25.40630420519635
28	30.83927510913137	20.075219290533393	24.35136611724125	24.734139483093987
29	33.09272464398306	20.04503710497782	24.355600181642465	22.50663806939665
30	28.82851447819741	21.15576459111142	22.43631866009577	27.5794022705954
31	27.912853510215868	20.308096542968297	28.208321098335272	23.570728848480567
32	28.11629314822569	18.920854858072815	25.40578252635258	27.55706946734891
33	26.948427214347408	19.558558648764958	27.06788020388763	26.425133933000005
34	25.988628661820336	18.148270286824662	30.10004891983526	25.763052131519743
35	28.329842460690703	18.835511605691615	24.429303692072512	28.405342241545167
36	27.992158883050394	19.34714012795206	25.37339811225628	27.287302876741265
37	24.894103363311107	20.397213075139323	27.057432181930015	27.651251379619556
38	29.831869852436977	21.22632892894112	22.479613073882206	26.462188144739702
39	27.81377099600879	21.99444024807986	24.45500657169146	25.736782184219887
40	29.089563180958898	20.323317988557914	22.452711183801057	28.134407646682135
41	27.148298444998282	21.492863263589907	23.313124704757556	28.04571358665426
42	27.604263034363314	21.981679740193062	24.71823858853403	25.69581863690959
43	24.99150005277393	24.220195738564637	24.58823013242912	26.200074076232312
44	24.30076219111373	23.015436606185645	25.24385327617254	27.439947926528085
45	26.249891002440386	20.385787415810285	26.65262971872235	26.71169186302698
46	25.113112870216792	21.65505275401566	24.101302591545686	29.130531784221862
47	28.283077157308927	20.27943642168721	24.220484195868615	27.217002225135257
48	24.23087277973754	22.980768271982488	24.415374030378967	28.372984917901007
49	27.54895502353678	21.49887466456241	21.465460909557816	29.486709402342992
50	24.644657270882394	25.72810546606786	23.892977575236632	25.734259687813115
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	7352.0
1	7239.0
2	5461.0
3	3740.5
4	3552.5
5	2873.5
6	2085.5
7	2002.0
8	2312.0
9	2572.5
10	2764.0
11	2879.5
12	2874.5
13	2802.0
14	2825.0
15	3086.0
16	3432.5
17	4091.5
18	4979.5
19	6063.5
20	7396.5
21	9457.5
22	11835.5
23	15049.0
24	18744.0
25	22383.5
26	27278.0
27	33903.5
28	41265.0
29	48456.5
30	56336.0
31	68254.0
32	84163.0
33	98109.0
34	113040.0
35	132638.0
36	151575.5
37	168872.5
38	184699.5
39	207851.5
40	239371.0
41	294212.5
42	379986.5
43	476233.5
44	528001.5
45	503419.5
46	495484.0
47	533027.5
48	600739.0
49	695018.5
50	775845.0
51	842656.5
52	918016.0
53	960705.5
54	936144.5
55	889104.5
56	855453.5
57	872477.5
58	917173.0
59	881201.0
60	798722.5
61	747780.0
62	703964.5
63	661891.5
64	640580.5
65	627429.5
66	568605.0
67	499306.5
68	441989.0
69	382164.5
70	333177.5
71	299703.5
72	278498.0
73	250031.5
74	207219.0
75	169227.5
76	144335.5
77	115008.5
78	90120.5
79	72692.0
80	55790.5
81	41806.0
82	31603.0
83	24008.0
84	18320.0
85	14021.5
86	10221.0
87	6888.5
88	4542.5
89	3221.5
90	2241.0
91	1447.0
92	1025.0
93	845.0
94	532.5
95	213.0
96	128.5
97	117.0
98	73.5
99	30.0
100	30.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	107197.0
25	105328.0
26	92974.0
27	88464.0
28	81408.0
29	74098.0
30	90183.0
31	76320.0
32	84983.0
33	77894.0
34	77121.0
35	83314.0
36	76109.0
37	77637.0
38	82417.0
39	91764.0
40	96874.0
41	95887.0
42	107833.0
43	111410.0
44	108123.0
45	161089.0
46	610834.0
47	1310532.0
48	1999558.0
49	2552967.0
50	1966130.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.86093773869952
#Duplication Level	Percentage of deduplicated	Percentage of total
1	55.69637556442374	10.504858717918216
2	13.56935925474711	5.1186168011566275
3	7.296551478810607	4.128594094471884
4	4.84938050045673	3.6585545476151164
5	3.4508034440073025	3.2542694452955803
6	2.5878259349398967	2.9285294302495966
7	2.034447555906073	2.68601120792157
8	1.58251840516057	2.387822488806366
9	1.231511598154824	2.090471721955603
>10	6.936328959466481	22.76467718911889
>50	0.4190967511598294	5.4879420888235515
>100	0.2862392320594587	10.658863028780093
>500	0.030904624326938922	4.052990699453458
>1k	0.023451369138409492	8.64524969805802
>5k	0.0031231963453242553	4.303929805276893
>10k+	0.002082130896882837	7.328619035098536
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA	43938	0.41891803248678927	No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCC	38926	0.3711321255537521	No Hit
ATCCGAATTCAGTCTTCCTGAGGCTCTGGCTCACCTCCAGGCAGGTCCC	32556	0.3103986404852272	No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTG	29587	0.2820913065498346	No Hit
ACTACTTCTCCCCCGGACTCCTTGGTAGTCTGTTAGTGGGAGATCCTTG	26394	0.25164828962302144	No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCCG	25518	0.2432962436387157	No Hit
ATTCCTGCTCACCGCAGTGTAGACAGGGAAACTTTCTTTGCAGTTCAGG	25186	0.24013085634786005	No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA	23806	0.2269735236328578	No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGA	22918	0.21850706605972592	No Hit
ACTACTTCTCCCCCGGACTCCTTGGTAGTCTGTTAGTGGGAGATCCTTGT	21354	0.20359542231605668	No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA	21273	0.20282314409148045	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA	20755	0.1978843771738202	No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGAC	20039	0.19105781904052915	No Hit
ATCCGAATTCAGTCTTCCTGAGGCTCTGGCTCACCTCCAGGCAGGTCCCA	19612	0.18698667333813354	No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATC	18782	0.1790732051109945	No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGC	18503	0.1764131356707875	No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTAG	17890	0.17056861034158724	No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG	17621	0.1680038838920687	No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAAT	16524	0.1575447578135488	No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGT	16085	0.15335920052232704	No Hit
ATCCGAATTCAGTCTTCCTGAGGCTCTGGCTCACCTCCAGGCAGGTC	15491	0.14769582687543475	No Hit
ATTCCTGCTCACCGCAGTGTAGACAGGGAAACTTTCTTTGCAGTTCAGGA	15301	0.14588431005235475	No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTGC	14758	0.1407071856579734	No Hit
AGCGAGCGGCCTTGAGAGGCTCTGGCTCTTGCTTCTTAGGCGGCCCGAG	14676	0.13992537313432835	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAAT	14050	0.13395690191723314	No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG	13951	0.13301300630941776	No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTG	12937	0.12334522705361176	No Hit
AAACAGCCGGGGCTCCAGCGGGAGAACGATAATGCAAAGTGCTATGTTCT	12877	0.12277316910948122	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAAC	12805	0.12208669957652458	No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCC	12657	0.12067562331433593	No Hit
CTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAATCCT	12350	0.11774859350020135	No Hit
ACTACTTCTCCCCCGGACTCCTTGGTAGTCTGTTAGTGGGAGATCCT	12138	0.1157273220976068	No Hit
AGGCCTGGTTTGCGCTGGAGAGTGGTCTTGGTTGTGAGGGTCATAAGAT	11945	0.11388720237732025	No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGG	11875	0.11321980144250131	No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACC	11729	0.11182779377845034	No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAAG	11398	0.10867194078666358	No Hit
ATTCCTGCTCACCGCAGTGTAGACAGGGAAACTTTCTTTGCAGTTCA	10648	0.10152121648503191	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.10478194676657596	0.0	0.0	0.0	0.0
2	0.27760065168840997	0.0	0.0	0.0	0.0
3	0.39630267509549555	0.0	0.0	0.0	0.0
4	0.4967846529820237	0.0	0.0	0.0	0.0
5	0.6428787176138929	0.0	0.0	0.0	0.0
6	0.7576240069074089	0.0	0.0	0.0	0.0
7	0.8585731654483104	0.0	0.0	0.0	0.0
8	0.9344375831390879	0.0	0.0	0.0	0.0
9	1.0045718870894913	0.0	0.0	0.0	0.0
10	1.0721891360857203	0.0	0.0	0.0	0.0
11	1.1366600663892312	0.0	0.0	0.0	0.0
12	1.187373003136403	0.0	0.0	0.0	0.0
13	1.2273884563283337	0.0	0.0	0.0	0.0
14	1.270998340269218	8.580869161957994E-5	0.0	0.0	0.0
15	1.3165818241173528	8.580869161957994E-5	0.0	0.0	9.534299068842215E-6
16	1.3495228274002027	8.580869161957994E-5	0.0	0.0	9.534299068842215E-6
17	1.382854736944875	8.580869161957994E-5	0.0	0.0	9.534299068842215E-6
18	1.41542390256404	8.580869161957994E-5	0.0	0.0	9.534299068842215E-6
19	1.4729252602482275	8.580869161957994E-5	0.0	0.0	9.534299068842215E-6
20	1.4973902716588765	8.580869161957994E-5	0.0	0.0	4.767149534421108E-5
21	1.5324860265312847	8.580869161957994E-5	0.0	0.0	4.767149534421108E-5
22	1.549266392892447	8.580869161957994E-5	0.0	0.0	4.767149534421108E-5
23	1.56637092542195	8.580869161957994E-5	0.0	0.0	4.767149534421108E-5
24	1.582073915988333	8.580869161957994E-5	0.0	0.0	4.767149534421108E-5
25	1.6014285430980828	8.580869161957994E-5	0.0	0.0	4.767149534421108E-5
26	1.6417395595611477	8.580869161957994E-5	0.0	0.0	4.767149534421108E-5
27	1.6563461057346138	8.580869161957994E-5	0.0	0.0	4.767149534421108E-5
28	1.6722779194786492	1.0487728975726437E-4	0.0	0.0	4.767149534421108E-5
29	1.6858547613526806	1.0487728975726437E-4	0.0	0.0	4.767149534421108E-5
30	1.6989072167779256	1.0487728975726437E-4	0.0	0.0	4.767149534421108E-5
31	1.712245701175236	1.0487728975726437E-4	0.0	0.0	4.767149534421108E-5
32	1.7244305353852163	1.0487728975726437E-4	0.0	0.0	4.767149534421108E-5
33	1.737073015950501	1.0487728975726437E-4	0.0	3.813719627536886E-5	4.767149534421108E-5
34	1.7489241496930719	1.0487728975726437E-4	0.0	3.813719627536886E-5	4.767149534421108E-5
35	1.7615189587630125	1.0487728975726437E-4	0.0	3.813719627536886E-5	4.767149534421108E-5
36	1.7724929369912499	1.0487728975726437E-4	0.0	3.813719627536886E-5	4.767149534421108E-5
37	1.7821035104526428	1.0487728975726437E-4	0.0	3.813719627536886E-5	4.767149534421108E-5
38	1.788920534286865	1.0487728975726437E-4	0.0	3.813719627536886E-5	4.767149534421108E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAAGCCA	6515	0.0	149.62677	44
CTATACG	485	0.0	139.9737	44
ATTAGTC	1725	0.0	114.375244	44
ATGTTCT	2835	0.0	109.62832	44
CCAGAAT	3560	0.0	92.069695	44
TCCTTGT	5850	0.0	86.67229	44
GGTCCCA	5405	0.0	84.780556	44
CGTGCGC	3415	0.0	77.96348	44
TTAAGCC	5540	0.0	77.504074	43
AATCGCA	290	0.0	73.15436	44
TTCAGGA	5300	0.0	69.04805	44
ATCCTTG	6855	0.0	68.63064	43
AGGTCCC	7820	0.0	66.30164	43
AATCCCG	4095	0.0	65.843094	43
TATTAGT	1345	0.0	64.190475	43
TCGTGCG	4650	0.0	62.15452	43
CAATGAA	5570	0.0	61.337753	43
AATGAAG	4530	0.0	60.88122	44
CTATGCG	430	0.0	56.73716	44
ATTAAGC	7470	0.0	56.64444	42
>>END_MODULE