##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841170.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4486055
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.38651041951113	39.0	37.0	39.0	34.0	39.0
2	37.972159057345486	39.0	38.0	39.0	35.0	39.0
3	38.18333658414799	39.0	38.0	39.0	35.0	39.0
4	38.527344181023196	39.0	38.0	40.0	35.0	41.0
5	39.2666835337507	40.0	39.0	41.0	36.0	41.0
6	39.195507634213136	40.0	39.0	41.0	35.0	41.0
7	39.115507723378336	40.0	39.0	41.0	35.0	41.0
8	39.16207982291791	40.0	39.0	41.0	35.0	41.0
9	39.15640200577122	40.0	38.0	41.0	35.0	41.0
10	38.97427182680551	40.0	38.0	41.0	35.0	41.0
11	39.10398245228826	40.0	39.0	41.0	35.0	41.0
12	39.09857569735547	40.0	38.0	41.0	35.0	41.0
13	39.07904829521707	40.0	38.0	41.0	35.0	41.0
14	39.027945043027785	40.0	38.0	41.0	35.0	41.0
15	38.98820121465296	40.0	38.0	41.0	35.0	41.0
16	38.911620566399655	40.0	38.0	41.0	35.0	41.0
17	38.839788633888794	40.0	38.0	41.0	35.0	41.0
18	38.77952967585105	40.0	38.0	41.0	35.0	41.0
19	38.6494516897363	40.0	38.0	41.0	35.0	41.0
20	38.56139035299389	40.0	38.0	41.0	35.0	41.0
21	38.2564056838358	40.0	37.0	41.0	34.0	41.0
22	38.1789349439541	40.0	37.0	41.0	34.0	41.0
23	37.988000147122584	40.0	36.0	41.0	33.0	41.0
24	37.87771661292606	39.0	36.0	41.0	33.0	41.0
25	37.824343083743344	39.0	36.0	41.0	33.0	41.0
26	37.64882070967324	39.0	35.0	41.0	33.0	41.0
27	37.58138059882498	39.0	35.0	41.0	33.0	41.0
28	37.58815421939786	39.0	35.0	41.0	33.0	41.0
29	37.631829973268104	39.0	35.0	41.0	33.0	41.0
30	37.66766721791891	39.0	35.0	41.0	34.0	41.0
31	37.64377668629644	39.0	35.0	41.0	34.0	41.0
32	37.593486961091976	39.0	35.0	41.0	34.0	41.0
33	37.450991293309826	39.0	35.0	41.0	34.0	41.0
34	37.39415292401533	38.0	35.0	41.0	34.0	41.0
35	37.30185264007361	38.0	35.0	41.0	34.0	41.0
36	37.115107782031025	37.0	35.0	41.0	33.0	41.0
37	37.01074262353607	37.0	35.0	41.0	33.0	41.0
38	36.92276371557952	37.0	35.0	41.0	33.0	41.0
39	36.77167441589618	37.0	35.0	40.0	33.0	41.0
40	36.62437172810527	36.0	35.0	40.0	33.0	41.0
41	36.461935545105	36.0	35.0	40.0	33.0	41.0
42	36.37487048975955	36.0	35.0	40.0	33.0	41.0
43	36.27812904071337	35.0	35.0	40.0	33.0	41.0
44	36.21916065544746	35.0	35.0	39.0	33.0	41.0
45	36.118976415718095	35.0	35.0	39.0	33.0	41.0
46	35.99708566473982	35.0	35.0	39.0	33.0	41.0
47	35.86207348754062	35.0	35.0	39.0	33.0	41.0
48	35.79610514834896	35.0	35.0	39.0	33.0	41.0
49	35.928963224172136	35.0	35.0	39.0	33.0	41.0
50	36.07647438153747	36.0	35.0	39.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	26.0
14	251.0
15	1222.0
16	191.0
17	7.0
18	0.0
19	0.0
20	4.0
21	8.0
22	35.0
23	93.0
24	287.0
25	714.0
26	1872.0
27	4391.0
28	9480.0
29	18495.0
30	32811.0
31	54093.0
32	84393.0
33	133036.0
34	256423.0
35	293632.0
36	457707.0
37	681590.0
38	805350.0
39	845911.0
40	804033.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	51.312634374745734	18.23845227042468	30.44891335482958
2	29.04748158459939	23.25958999611017	27.170175131602264	20.522753287688182
3	32.78234439836337	14.077357500075232	21.56698925893686	31.57330884262453
4	23.203571957989816	16.77534047175079	26.236437136860786	33.78465043339861
5	26.285812367436424	21.18204079085076	28.68437859098919	23.84776825072363
6	30.984283518592616	21.976993148768795	21.796099245328023	25.242624087310567
7	33.47480135664855	20.691966549674493	22.85854720907345	22.974684884603512
8	31.950232442535814	17.500788554754678	26.161203997721827	24.387775004987677
9	26.841133245134085	20.230001638410585	26.189870610146333	26.738994506308995
10	30.90940704026143	19.642581288013634	25.000941807445514	24.447069864279417
11	29.3935094420376	23.22942986655313	23.395477763870485	23.981582927538785
12	26.59100256238499	16.763971908503127	28.79287035045268	27.8521551786592
13	24.28418733163102	16.955721675280397	25.991856987932604	32.768234005155975
14	24.829543998011616	22.1609409603761	20.947112775032853	32.06240226657943
15	23.62001803366209	22.073157819063745	26.291117696952	28.015706450322163
16	29.703113314482323	21.912459833862936	23.648327985278826	24.736098866375915
17	29.061279899599985	19.95124892583796	19.62871609911158	31.35875507545048
18	27.491905471511163	19.30025378645603	19.04479548289087	34.16304525914194
19	26.181645120267138	23.732031818602316	25.256734480517963	24.829588580612587
20	33.782688798955874	17.234518970453998	21.91386418579353	27.068928044796596
21	28.264053829032417	20.491612340909775	24.241967608511263	27.00236622154655
22	29.530467192221227	21.585268125335066	25.884680415197764	22.999584267245943
23	30.622518003011557	18.43731296205686	23.153661736202523	27.786507298729063
24	31.349972303059147	19.340890827241306	20.898696070378094	28.410440799321453
25	26.313193566047644	22.348940535756164	24.779944319669127	26.557921578527065
26	28.646111103103333	20.373624363982298	25.02234169825735	25.957922834657023
27	28.767998955458747	22.33488971793306	24.062508358823496	24.834602967784697
28	28.017805984712812	17.727272310032323	28.10893337580284	26.145988329452024
29	34.48035391746121	18.91032966529104	23.706396916346037	22.90291950090171
30	25.99028985552514	19.6155057653232	22.41576784840315	31.97843653074851
31	25.871027444280763	18.753394792499904	33.08479410455297	22.290783658666356
32	28.391836885165784	18.29337415715376	26.816462341408116	26.498326616272337
33	28.8245017697575	20.251695052819745	22.266966492818845	28.656836684603913
34	23.201946169458036	21.51584041038365	31.475467381515454	23.806746038642864
35	26.72380408761782	22.77145605700128	22.551427369374114	27.953312486006787
36	25.6521456387092	20.42877415140149	26.790162866723357	27.12891734316595
37	24.289924293728006	21.00379177742177	23.23492910181713	31.471354827033093
38	29.2875291737086	25.835396549991827	21.10354473368634	23.773529542613232
39	26.204075649615017	26.508589747322574	21.167193600886737	26.12014100217567
40	26.698005219392574	26.524336565563004	19.968237680624227	26.80942053442019
41	28.83759032255363	23.40642376615325	20.860706399581165	26.895279511711955
42	26.853817376632392	26.12140406223852	23.468022279594912	23.556756281534177
43	24.407276044509892	23.85678447705623	26.315524698078413	25.420414780355465
44	24.79207755807157	23.695100026427234	25.12847150350128	26.384350911999917
45	25.24259108054045	25.09395616043861	26.06303484957569	23.600417909445255
46	26.2422162279117	26.15495545441993	22.31661879976364	25.286209517904723
47	32.322251444603836	20.65831079973944	21.147385660615782	25.87205209504095
48	24.779235444569885	22.78791045235554	22.435136416036535	29.997717687038044
49	27.729550214727528	22.217605597659098	20.157254364426464	29.895589823186913
50	24.80426513760149	29.342470378178444	22.616369254756453	23.236895229463613
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2498.0
1	2509.5
2	3668.0
3	4685.5
4	4329.0
5	3071.5
6	1781.5
7	1586.5
8	1711.5
9	1814.5
10	1807.5
11	1753.0
12	1742.5
13	1810.0
14	1932.5
15	2230.0
16	2543.0
17	3031.0
18	3502.5
19	4589.5
20	5726.5
21	7075.0
22	8570.0
23	10715.5
24	12821.5
25	14472.5
26	16703.5
27	20108.0
28	23485.5
29	27134.5
30	31016.5
31	35721.5
32	41250.5
33	48035.0
34	54913.0
35	60137.5
36	67570.5
37	78625.5
38	101381.0
39	136327.0
40	164990.5
41	189054.0
42	214011.0
43	298124.0
44	358118.5
45	277140.5
46	209693.0
47	210000.5
48	227026.5
49	258936.0
50	306958.5
51	352073.5
52	370645.5
53	372125.0
54	359487.0
55	324390.5
56	293411.0
57	297460.5
58	320902.5
59	320803.5
60	285360.0
61	259599.5
62	251412.5
63	243828.0
64	257318.0
65	275672.5
66	242891.5
67	197084.0
68	169625.0
69	149621.5
70	133107.5
71	121006.0
72	119147.5
73	110804.0
74	90149.0
75	74101.0
76	66871.0
77	52528.5
78	39777.5
79	36644.5
80	33188.5
81	23051.0
82	14365.5
83	12334.0
84	10210.0
85	7789.0
86	5760.5
87	3653.0
88	1864.0
89	1269.0
90	836.0
91	503.0
92	342.0
93	282.0
94	196.0
95	89.0
96	49.5
97	41.0
98	36.5
99	31.5
100	31.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	25593.0
25	20334.0
26	28622.0
27	53870.0
28	82967.0
29	112630.0
30	17824.0
31	21132.0
32	20261.0
33	17669.0
34	20683.0
35	20243.0
36	21839.0
37	21354.0
38	16982.0
39	19606.0
40	23985.0
41	27643.0
42	36646.0
43	35437.0
44	29383.0
45	30730.0
46	51745.0
47	122886.0
48	546998.0
49	1181070.0
50	1877923.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	13.065203887340978
#Duplication Level	Percentage of deduplicated	Percentage of total
1	46.103522653637626	6.023519233944192
2	10.404429630818141	2.718719889162617
3	6.515545560394344	2.553807935514343
4	5.428936524487323	2.837206503354368
5	4.539385960821443	2.965400155073269
6	3.714569824397248	2.911896726570865
7	3.1537206487963645	2.8842802296169245
8	2.7856072398071112	2.9115621230506434
9	2.225991089947752	2.6174724697414575
>10	14.152723271933013	31.891906165910527
>50	0.5481717194147312	4.890011797756095
>100	0.3532243939403074	9.037342325618653
>500	0.035209112954521005	3.2526509375107087
>1k	0.030562224950288902	8.082590292855658
>5k	0.0050043409275326856	4.561566581041405
>10k+	0.0033958027722543224	9.860066633278246
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA	138888	3.0959941418462327	No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCCG	23176	0.5166231800546359	No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCC	20786	0.46334697189401375	No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC	18956	0.4225538920053365	No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTTCTACCGGGAAGCAGTGGT	18418	0.41056117234407513	No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAAG	18297	0.4078639249853156	No Hit
CTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAATCCTG	16960	0.37806045623604706	No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA	16372	0.3649531715505049	No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA	16355	0.3645742194422494	Illumina Single End Adapter 1 (100% over 32bp)
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA	16197	0.3610521939655221	Illumina Single End Adapter 1 (100% over 32bp)
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGAC	14797	0.3298443732856597	No Hit
CTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAATCCT	14670	0.32701337812398645	No Hit
CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGG	14659	0.32676817381864465	No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGC	14600	0.3254529870899933	No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATC	13824	0.30815493791315535	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA	13812	0.3078874423073279	No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGC	10794	0.2406122974417389	No Hit
ATCCGAATTCAGTCTTCCTGAGGCTCTGGCTCACCTCCAGGCAGGTCCC	10583	0.23590883303927393	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAAT	10111	0.22538733921006318	No Hit
AAGCAGTGGTATCAACGCAGAGTGCAG	9127	0.20345269953221706	No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCC	8865	0.1976123788049857	No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTAG	8806	0.1962971920763343	No Hit
CAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGCGC	8588	0.19143768857047005	No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCG	8486	0.1891639759209372	No Hit
CGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGT	8370	0.18657818506460577	No Hit
ATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGC	8313	0.18530758093692565	No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGA	8270	0.18434905501604418	No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTG	8221	0.18325678129224898	No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAAT	8148	0.18162951635679903	No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA	7519	0.16760828835134656	Illumina Single End Adapter 1 (100% over 32bp)
TCGCTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAATC	7124	0.15880322465952826	No Hit
ATCCGAATTCAGTCTTCCTGAGGCTCTGGCTCACCTCCAGGCAGGTCCCA	6841	0.15249478662209892	No Hit
ACTACTTCTCCCCCGGACTCCTTGGTAGTCTGTTAGTGGGAGATCCTTGT	6733	0.1500873261696524	No Hit
CTCTGCAGGTACCATGGAGCTGAGCTATAGGCTCTTCATCTGCCTCCTG	6600	0.14712258320506547	No Hit
TACCTGGTTGATCCTGCCAGTAGCATATG	6465	0.1441132576395073	No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTTCTACC	6232	0.1389193846263588	No Hit
AGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTCGCT	6130	0.13664567197682595	No Hit
TCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA	6086	0.13566485475545886	Illumina Single End Adapter 1 (100% over 31bp)
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTTCTACCGGGAAGCAGTGG	5980	0.13330197690398357	No Hit
AAGCAGTGGTATCAACGCAGAGTG	5839	0.1301589035355117	No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA	5748	0.12813039519132066	Illumina Single End Adapter 1 (100% over 32bp)
TCGCTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAAT	5658	0.12612417814761523	No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTTCTAC	5579	0.12436316540925156	No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA	5556	0.1238504654980824	No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA	5514	0.1229142308776865	Illumina Single End Adapter 1 (100% over 32bp)
AGGCCTGGTTTGCGCTGGAGAGTGGTCTTGGTTGTGAGGGTCATAAGATG	5462	0.12175508325243448	No Hit
ACTACTTCTCCCCCGGACTCCTTGGTAGTCTGTTAGTGGGAGATCCTTG	5088	0.11341813687081412	No Hit
CCCTTCCACCTTCTGCCGTGTTAGGATTTGGTGTTCCTCCCTTCCAGAA	4734	0.10552701649890604	No Hit
TCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA	4684	0.10441245147462526	Illumina Single End Adapter 1 (100% over 31bp)
AGGCCTGGTTTGCGCTGGAGAGTGGTCTTGGTTGTGAGGGTCATAAGAT	4642	0.10347621685422939	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.13720295448896636	0.0	0.0	0.0	0.0
2	0.23831629349172045	0.0	0.0	0.0	0.0
3	0.3137277630345593	0.0	0.0	0.0	0.0
4	0.42050309236065986	0.0	0.0	0.0	0.0
5	0.5493691004680058	0.0	0.0	0.0	0.0
6	0.6975616660963809	0.0	0.0	0.0	0.0
7	0.8133203895181846	0.0	0.0	0.0	0.0
8	0.9357665030856732	0.0	0.0	0.0	0.0
9	1.0194480451086756	0.0	0.0	0.0	0.0
10	1.099652144255922	0.0	0.0	0.0	0.0
11	1.1828655689687264	0.0	0.0	0.0	0.0
12	1.2410012806352129	0.0	0.0	0.0	0.0
13	1.2996051096118972	0.0	0.0	0.0	0.0
14	1.3647625809313528	0.0	0.0	0.0	0.0
15	1.4357826642785254	0.0	0.0	0.0	0.0
16	1.4897499027542016	0.0	0.0	0.0	0.0
17	1.57365435778206	0.0	0.0	0.0	0.0
18	1.6179471718469791	0.0	0.0	0.0	0.0
19	1.689413081203864	0.0	0.0	0.0	0.0
20	1.7211781843958667	0.0	0.0	0.0	0.0
21	1.7600096298418098	0.0	0.0	0.0	0.0
22	1.7844631864745306	0.0	0.0	0.0	2.229130048561598E-5
23	1.8153589289475942	0.0	0.0	0.0	2.229130048561598E-5
24	1.8459203019133739	0.0	0.0	0.0	2.229130048561598E-5
25	1.8761027227708977	0.0	0.0	0.0	2.229130048561598E-5
26	1.9349294647524384	0.0	0.0	0.0	2.229130048561598E-5
27	1.956953269632227	0.0	0.0	0.0	2.229130048561598E-5
28	1.989922103050453	0.0	0.0	0.0	2.229130048561598E-5
29	2.038338807705211	0.0	0.0	0.0	2.229130048561598E-5
30	2.0564170523990457	0.0	0.0	0.0	2.229130048561598E-5
31	2.0706389021088687	0.0	0.0	0.0	2.229130048561598E-5
32	2.0836347302919824	0.0	0.0	0.0	2.229130048561598E-5
33	2.0966974323765535	0.0	0.0	0.0	2.229130048561598E-5
34	2.1105849125790925	0.0	0.0	0.0	2.229130048561598E-5
35	2.123068040851037	0.0	0.0	0.0	2.229130048561598E-5
36	2.1366880254477487	0.0	0.0	0.0	2.229130048561598E-5
37	2.149327192823093	0.0	0.0	0.0	2.229130048561598E-5
38	2.159893269253275	0.0	0.0	0.0	2.229130048561598E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAAGCCA	16260	0.0	91.225464	44
ATTAGTC	2335	0.0	85.217445	44
AATGAAG	3485	0.0	58.64008	44
TTAAGCC	16205	0.0	56.500416	43
TATTAGT	2335	0.0	52.742897	43
CTATACG	205	0.0	52.467434	44
AATCCCG	3135	0.0	50.725666	43
CCAGAAT	2390	0.0	49.708355	44
CAATGAA	4820	0.0	48.80692	43
ATTAAGC	16245	0.0	48.07718	42
AGATTAA	16545	0.0	45.35592	40
GATTAAG	16895	0.0	45.268944	41
AATCCTG	3695	0.0	44.97944	43
AAGATTA	16640	0.0	44.799225	39
TAATCCT	5720	0.0	44.24031	42
AAAGATT	17030	0.0	43.527767	38
CTAATCC	11150	0.0	43.21222	41
CAAAGAT	17065	0.0	43.060352	37
CGCTACC	660	0.0	42.96404	44
CTCAAAG	16730	0.0	42.952354	35
>>END_MODULE