FastQCFastQC Report
Tue 24 May 2016
ERR841169.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841169.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3136146
Sequences flagged as poor quality0
Sequence length24-50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA2587148.249424612247006No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGC353741.1279449362370246No Hit
CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGG261650.8343042702731315No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGC236940.7555132956182524No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC223670.7132002145308286No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA194330.6196458965877226No Hit
CAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGCGC144750.46155376694835004No Hit
ATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGC133890.4269252770757484No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCG133320.4251077596514958No Hit
CGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGT132560.4226844030858257No Hit
TACCTGGTTGATCCTGCCAGTAGCATATG119570.3812641375752277No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCC100470.3203613607274661No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA96670.3082445778991157Illumina Single End Adapter 1 (100% over 32bp)
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA95810.30550235862743635Illumina Single End Adapter 1 (100% over 32bp)
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA89190.2843936474896258No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTGTAATGGGGAAGCAGTGGT86800.27677282881600535No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGAC83840.26733449271813237No Hit
AGGCCTGGTTTGCGCTGGAGAGTGGTCTTGGTTGTGAGGGTCATAAGAT82410.26277475602220046No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCC79840.2545799844777635No Hit
AACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTT79100.25222040045329525No Hit
ATCCGAATTCAGTCTTCCTGAGGCTCTGGCTCACCTCCAGGCAGGTCCC75930.24211245267280285No Hit
AAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCGAATG73950.23579897109382025No Hit
CAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGG70320.22422425486568545No Hit
ACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCAT68530.2185166124281204No Hit
AGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTCGCT68270.2176875693924964No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCCG66140.21089579375449993No Hit
CTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAATCCT63430.20225461442165No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG60710.19358154881819917No Hit
TCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGC57540.1834736010377068No Hit
CGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGGGGAATCA56860.18130533463684406No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGA54210.17285547292759967No Hit
CGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTG51110.16297072904131377No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAAG50650.16150396059367134No Hit
ATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAAT50210.16010096468723076No Hit
TCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA50120.15981398825182247Illumina Single End Adapter 1 (100% over 31bp)
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAAT49370.1574225179567533No Hit
CTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAATCCTG49120.15662536119173023No Hit
CGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTC48560.15483973003807858No Hit
AAGCAGTGGTATCAACGCAGAGTGCAG44770.14275483348032905No Hit
ACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTCG43410.1384183006786036No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTAG43210.13778057526658516No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA41750.13312517975885052Illumina Single End Adapter 1 (100% over 32bp)
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAAT41710.13299763467644685No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGT41240.13149897995820348No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCT40930.1305105055695749No Hit
ATCAGATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGG40860.13028730167536842No Hit
ATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGGGGAATCAG40850.1302554154047675No Hit
AAGCAGTGGTATCAACGCAGAGTG38530.12285780062535354No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA37900.12084896557749544No Hit
CGAGGCCCTGTAATTGGAATGAGTCCACTTTAAATCCTTTAACGAGGATC37260.11880824425903641No Hit
TTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAATTC36700.11702261310538475No Hit
TCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA35390.11284551165666394Illumina Single End Adapter 1 (100% over 31bp)
CGCTGACCCCCTTCGCGGGGGGGATGCGTGCATTTATCAGATCAAAA34700.11064535898520031No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA33010.10525657925364443Illumina Single End Adapter 1 (100% over 32bp)
TGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGAC32730.10436376367681861No Hit
CCCTTCCACCTTCTGCCGTGTTAGGATTTGGTGTTCCTCCCTTCCAGA32300.10299265404097896No Hit
AGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGT31880.10165343067574022No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA31820.10146211305213468Illumina Single End Adapter 1 (100% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGCCA299550.085.76681544
ATTAGTC25450.080.1895444
CTATACG3450.074.4413844
TTAAGCC300350.056.264743
TATTAGT25600.052.5469643
AATCCCG10050.050.1941143
CAATGAA30700.048.80583243
ATTAAGC300800.047.31574642
ATAAGAT16750.045.71249443
AGGTCCC15750.045.37388243
GATTAAG305700.045.12117841
CGACGGG58450.045.11581844
AGATTAA304350.044.62545440
AAGATTA304950.044.28455739
AAAGATT306850.043.72353438
TCGTGCG12850.043.46296743
CAAAGAT308000.043.13894737
ATATTAG26500.042.87880342
CTCAAAG305250.042.8080535
CTAATCC43900.042.73567641
Produced by FastQC (version 0.11.5)