FastQCFastQC Report
Tue 24 May 2016
ERR841168.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841168.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4893257
Sequences flagged as poor quality0
Sequence length24-50
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA1111692.27188148915947No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCC893541.826063907945158No Hit
CTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAATCCT564651.1539348944884766No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGC411040.8400131037466457No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGC383070.782852811532278No Hit
CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGG379690.7759453468313641No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAAT378900.7743308802296711No Hit
ATCCGAATTCAGTCTTCCTGAGGCTCTGGCTCACCTCCAGGCAGGTCCC346100.7072998618302697No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA326750.6677556482318423No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCCG251430.5138295413463876No Hit
CGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGT226500.4628818801056229No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATC203850.4165936920950606No Hit
ATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGC199310.40731561820685075No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA195180.3988754320486334No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCG194500.39748576459401175No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGA185860.3798288134058767No Hit
TCGCTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAAT185320.37872525395661827No Hit
CTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAATCCTG179120.3660547565762436No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA158470.323853825785157Illumina Single End Adapter 1 (100% over 32bp)
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA154780.3163128362152243Illumina Single End Adapter 1 (100% over 32bp)
CAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGCGC149560.3056450948723928No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCC145940.29824715930514173No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA137960.2819390029994337No Hit
CTGTGCGTCTGCTCCTCCTTCTCGCGCTTCTCCTGCCGCCCTAATCCT114030.23303497036840695No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGAC111310.22747630054992No Hit
AGGCCTGGTTTGCGCTGGAGAGTGGTCTTGGTTGTGAGGGTCATAAGAT99820.20399500782403213No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG95510.19518696851606201No Hit
CGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTG90550.18505057061176225No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTG85160.17403541240527526No Hit
ACTACTTCTCCCCCGGACTCCTTGGTAGTCTGTTAGTGGGAGATCCTTG82940.16949855689165721No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGT79340.16214149389660099No Hit
CCCTTCCACCTTCTGCCGTGTTAGGATTTGGTGTTCCTCCCTTCCAGA75990.1552953380539792No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAAG72990.14916445222476563No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTAG71730.14658948017649595No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC70450.1439736355560315No Hit
TCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGC69480.14199131580458577No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGGTTGTGAGGGTTCCTGCTT68570.14013161376972436No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA67440.13782231344072057Illumina Single End Adapter 1 (100% over 32bp)
ACACCCGGGTGAGGGGTCTGCTGGCTGCACCTGTCGGTCTCATGGCTCC65050.13293804106344712No Hit
TCTGGAAGAGGTCTTGCGAAAGCCGCCTCGAGCGCGCTCCGCGGCTGC64110.13101703017029354No Hit
CGGCTCCTGGCCAGGTAACTTACTACTTCCAGTCAATATGCCGACCTT63410.12958649014347703No Hit
TCGCTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAATC62480.12768591553642084No Hit
AGCGAGCGGCCTTGAGAGGCTCTGGCTCTTGCTTCTTAGGCGGCCCGAG60040.12269946172866047No Hit
TTGTCTTTCTAGGTCTCAGCCGGTCGTCGCGACGTTCGCCCGCTCGCT59430.12145284827672038No Hit
CGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTC59020.12061496054672788No Hit
CGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGGGGAATCA56690.11585330588603868No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA53170.10865973317976145Illumina Single End Adapter 1 (100% over 32bp)
CTCTGCAGGTACCATGGAGCTGAGCTATAGGCTCTTCATCTGCCTCCT52760.10782184544976894No Hit
CTCTTTTTAAGTTAGTGCTGGAACGTGGAAGAGCTGCTGCCTCCGAAG51830.10592127084271274No Hit
TCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA51730.10571690798173897Illumina Single End Adapter 1 (100% over 31bp)
TACCTGGTTGATCCTGCCAGTAGCATATG51540.10532861854588876No Hit
TCGCTGTGCGTCTGCTCCTCCTTCTCGCGCTTCTCCTGCCGCCCTAAT50850.10391851480516966No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA50050.10228361191737938Illumina Single End Adapter 1 (100% over 32bp)
ACCAGCGAGCGGCCTTGAGAGGCTCTGGCTCTTGCTTCTTAGGCGGCCC49940.1020588127703082No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGCCA126850.0165.6525644
ATTAGTC10200.0145.690644
CGACGGG65900.0110.54993444
AATGAAG19500.076.6720744
TTAAGCC125350.070.14493643
CAATGAA50900.065.89506543
AGGTCCC52950.061.00306743
GGCGGGC70650.059.51051344
AATCGCA1403.092282E-1158.2508644
CGGGCGC60250.056.39778544
TATTAGT11000.056.33917243
AATCCCG40200.055.12456543
ACTACAC4950.054.91663744
AATCCTG35750.052.42563243
ATAAGAT19100.051.32469643
CTATCAT6100.050.505344
CTATACG550.00882522449.42497344
GACGGGC29200.048.71970744
TCGTGCG17100.048.1024543
ATCCTTG14850.048.05586243
Produced by FastQC (version 0.11.5)