##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841167.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2269816
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.196069196798334	39.0	35.0	39.0	32.0	39.0
2	36.97050245482453	39.0	35.0	39.0	33.0	39.0
3	37.19772219422191	39.0	35.0	39.0	35.0	39.0
4	37.579117866822685	39.0	35.0	39.0	35.0	41.0
5	38.24106227112682	40.0	35.0	41.0	35.0	41.0
6	38.23263207237944	40.0	35.0	41.0	35.0	41.0
7	38.187895847064254	40.0	35.0	41.0	35.0	41.0
8	38.20966941813786	40.0	35.0	41.0	35.0	41.0
9	38.19989241418688	40.0	35.0	41.0	35.0	41.0
10	38.15954861539438	40.0	35.0	41.0	35.0	41.0
11	38.18856286148304	40.0	35.0	41.0	35.0	41.0
12	38.157766973181964	40.0	35.0	41.0	34.0	41.0
13	38.14678855026134	40.0	35.0	41.0	34.0	41.0
14	38.13248562879106	40.0	35.0	41.0	35.0	41.0
15	38.13381877649994	40.0	35.0	41.0	35.0	41.0
16	38.1157080573932	40.0	35.0	41.0	34.0	41.0
17	38.05498991988778	40.0	35.0	41.0	34.0	41.0
18	38.01479326958661	40.0	35.0	41.0	34.0	41.0
19	37.87435016759068	40.0	35.0	41.0	34.0	41.0
20	37.85983225071988	40.0	35.0	41.0	34.0	41.0
21	37.66476533780712	39.0	35.0	41.0	34.0	41.0
22	37.56506826985095	39.0	35.0	41.0	33.0	41.0
23	37.47194221910498	39.0	35.0	41.0	33.0	41.0
24	37.3832037486739	39.0	35.0	41.0	33.0	41.0
25	37.3401151196459	39.0	35.0	41.0	33.0	41.0
26	37.24108303415999	38.0	35.0	41.0	33.0	41.0
27	37.20250951771115	38.0	35.0	41.0	33.0	41.0
28	37.303601090200885	38.0	35.0	41.0	33.0	41.0
29	37.340876482036045	38.0	35.0	41.0	33.0	41.0
30	37.34541071112944	38.0	35.0	41.0	34.0	41.0
31	37.30083276323919	38.0	35.0	41.0	33.0	41.0
32	37.29643935939816	38.0	35.0	41.0	34.0	41.0
33	37.25568057747412	38.0	35.0	41.0	34.0	41.0
34	37.199854829549125	37.0	35.0	41.0	34.0	41.0
35	37.08815629132454	37.0	35.0	41.0	34.0	41.0
36	36.98528949976967	37.0	35.0	41.0	34.0	41.0
37	36.927569927166616	36.0	35.0	41.0	33.0	41.0
38	36.840102536495586	36.0	35.0	41.0	33.0	41.0
39	36.72415580362911	36.0	35.0	40.0	33.0	41.0
40	36.573101576943564	35.0	35.0	40.0	33.0	41.0
41	36.43783583855059	35.0	35.0	40.0	33.0	41.0
42	36.37075794833809	35.0	35.0	40.0	33.0	41.0
43	36.28608216880883	35.0	35.0	40.0	33.0	41.0
44	36.213460393031404	35.0	35.0	40.0	33.0	41.0
45	36.112655558420734	35.0	35.0	39.0	33.0	41.0
46	36.00457340423204	35.0	35.0	39.0	33.0	41.0
47	35.94979684551984	35.0	35.0	39.0	33.0	41.0
48	35.91150652845718	35.0	35.0	39.0	33.0	41.0
49	36.234856461699586	35.0	35.0	39.0	33.0	41.0
50	36.82119965352967	37.0	35.0	40.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	3.0
14	79.0
15	551.0
16	221.0
17	2.0
18	0.0
19	0.0
20	7.0
21	20.0
22	60.0
23	186.0
24	452.0
25	971.0
26	1974.0
27	3687.0
28	6865.0
29	12061.0
30	20575.0
31	34066.0
32	59011.0
33	115843.0
34	396864.0
35	125336.0
36	131933.0
37	217027.0
38	319870.0
39	377381.0
40	444771.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	49.41669280681782	15.432087887300117	35.151219305882066
2	29.972209201098238	20.53805242363258	28.75396067346428	20.735777701804903
3	32.5166885774001	13.739924293422904	22.47887053399923	31.26451659517776
4	22.099456519823633	17.451987297648795	28.39379932117846	32.05475686134911
5	26.221376534485614	19.864341426794066	29.658879838718207	24.255402200002116
6	29.372513014270762	24.238043964797146	21.882038015416228	24.507405005515864
7	33.967378853616324	21.035229287307867	21.90670961875324	23.090682240322565
8	30.84073775143007	17.811443746982132	25.379986747824496	25.9678317537633
9	25.24222227704801	21.95371783439715	26.715205109136598	26.08885477941824
10	31.245968836240472	18.801127492272503	23.442252587874965	26.510651083612064
11	31.479732277858645	20.59933492406433	24.693895892882946	23.227036905194076
12	28.623157119343595	16.912031636044507	25.837380651118856	28.62743059349304
13	24.82989810627822	16.665403715543462	28.725059652412355	29.779638525765968
14	23.86933566421243	21.776699080454097	22.0080394181731	32.345925837160365
15	24.75059652412354	20.779481684859036	26.54215143430128	27.92777035671614
16	28.725015595977826	23.491727963852576	23.757564489808868	24.025691950360734
17	28.331767861359687	20.454257085155803	21.19211425067054	30.021860802813972
18	28.759952348560414	20.68929816337536	20.224590891948953	30.32615859611528
19	26.555280251791334	22.35097470455755	26.34799472732591	24.7457503163252
20	33.78119636129096	17.579927183525008	21.04320350195787	27.595672953226163
21	29.15712110585175	19.41915115586462	23.42062087852055	28.003106859763083
22	28.553547952785603	21.632942934581482	25.26975754862949	24.543751564003426
23	30.91074342589884	19.079960666415253	21.303004296383495	28.706291611302415
24	30.909201450690276	20.048144871654795	21.02822431421754	28.014429363437387
25	27.918572527014785	20.653941207795697	26.09890657141681	25.328579693772713
26	29.51000474765418	18.895574723987032	25.266855505945234	26.327565022413552
27	27.568283510819843	21.977134232940248	24.233114701447374	26.22146755479254
28	28.85877074954031	17.89451780552644	25.77961512934802	27.46709631558523
29	34.93776960007236	19.478722044315592	22.745973795346	22.837534560266043
30	27.565380309903965	21.998326754256762	21.23678783950981	29.199505096329464
31	26.727337930042587	19.5343111512306	30.490621596585303	23.24772932214151
32	28.173562825335306	18.365059462745545	24.472909305064782	28.988468406854363
33	29.44131095017188	20.66791900486791	22.16333477956146	27.727435265398753
34	24.42873226578346	21.800735613220752	27.686694032460956	26.08383808853484
35	26.60334402005105	23.031174078174494	22.874092782236985	27.491389119537473
36	26.790890169283866	20.939972843192347	26.89218554418528	25.376951443338502
37	26.339801655757554	20.305180742316615	23.183171871391437	30.17184573053439
38	28.578161242331866	24.226627810595115	21.528777170665155	25.66643377640786
39	26.460384755097117	25.541136517115532	20.488172352340296	27.51030637544706
40	27.283894886096576	26.354610581894526	19.953271394102718	26.408223137906184
41	28.53581892593636	22.695935434400784	21.30996278201831	27.45828285764454
42	29.447506627220633	22.84644379953727	22.62170425264582	25.08434532059628
43	24.9834943402765	22.98006076472738	25.272281146901864	26.764163748094255
44	27.05344769842591	22.388029769150705	23.84705707759352	26.711465454829863
45	27.3104598135595	23.191571068770433	26.43946890749178	23.05850021017828
46	27.788964900254804	24.66961518164613	22.864476704746988	24.676943213352082
47	33.44042732779452	19.154395336135842	20.646142961173084	26.75903437489656
48	24.82978250107408	23.345640752751724	22.112899673086446	29.71167707308775
49	27.82653040438261	22.726458545672426	20.69158564708312	28.755425402861846
50	26.72419942424783	28.650039487427716	21.702799786003617	22.92296130232084
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	915.0
1	1093.0
2	2096.0
3	2783.5
4	2506.5
5	1772.5
6	1041.0
7	921.5
8	947.5
9	993.5
10	1046.0
11	1049.0
12	1000.0
13	944.5
14	925.5
15	1047.5
16	1186.5
17	1446.5
18	1747.0
19	2290.0
20	2856.0
21	3631.5
22	4449.0
23	5448.5
24	6395.5
25	7556.0
26	9058.0
27	10620.0
28	12211.5
29	13906.0
30	15869.0
31	19083.0
32	22574.0
33	25410.5
34	28169.5
35	31006.0
36	35706.5
37	41768.5
38	50482.5
39	64215.0
40	76351.0
41	88831.0
42	110648.5
43	146726.0
44	157788.5
45	128864.5
46	112772.0
47	107400.5
48	106795.0
49	118940.0
50	136140.5
51	150462.5
52	157105.5
53	164714.5
54	165472.5
55	151569.0
56	142149.5
57	150180.0
58	166773.5
59	170269.0
60	155111.5
61	140500.5
62	133152.0
63	128757.5
64	136732.0
65	145405.5
66	126177.5
67	103953.0
68	90859.5
69	81416.0
70	73482.0
71	71924.0
72	78074.5
73	67565.0
74	48331.0
75	42378.0
76	41747.5
77	31372.5
78	22094.0
79	20709.5
80	19214.0
81	12974.5
82	7766.0
83	6977.5
84	6322.0
85	5191.5
86	4063.5
87	2432.5
88	1085.0
89	719.0
90	465.0
91	275.5
92	170.0
93	140.0
94	96.5
95	43.0
96	26.0
97	25.0
98	22.0
99	19.0
100	19.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	10775.0
25	9509.0
26	13963.0
27	29737.0
28	38965.0
29	45242.0
30	7939.0
31	8166.0
32	8110.0
33	7453.0
34	8869.0
35	7943.0
36	7746.0
37	7205.0
38	7279.0
39	8483.0
40	9275.0
41	10816.0
42	13939.0
43	13854.0
44	12897.0
45	16594.0
46	31553.0
47	99380.0
48	410497.0
49	638567.0
50	785060.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	12.832004685507576
#Duplication Level	Percentage of deduplicated	Percentage of total
1	44.81857555746217	5.751121715511298
2	10.042188289667749	2.577228143715317
3	6.702296145166846	2.58011686615321
4	5.4213750926933635	2.782684423653412
5	4.502499278745623	2.8888045920679155
6	3.8287249895864113	2.9478130203535664
7	3.198650131721703	2.873156543428662
8	2.7253677886680796	2.797754578713617
9	2.3206180611575586	2.680036365064257
>10	15.37422790760685	34.611251850468705
>50	0.6217577662016847	5.495034093266145
>100	0.362976692307476	9.203194023684128
>500	0.043644485953737606	3.8256292513087424
>1k	0.030187436118001846	8.16314077352249
>5k	0.004364448595373761	3.8713663615828953
>10k+	0.0025459283473013607	6.951667397505701
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA	47357	2.0863805700550175	No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA	22207	0.9783612416160605	No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGC	20544	0.9050953909920452	No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGC	18270	0.80491105886997	No Hit
CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGG	17463	0.7693575162039566	No Hit
ATCCGAATTCAGTCTTCCTGAGGCTCTGGCTCACCTCCAGGCAGGTCCC	12932	0.5697378113468228	No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCC	10179	0.4484504470846976	No Hit
ATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGC	9882	0.43536568602917597	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA	9828	0.43298663856453556	No Hit
CGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGT	9366	0.41263256581150193	No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGA	7446	0.3280442115131799	No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCG	7287	0.32103923842285015	No Hit
CAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGCGC	6120	0.2696253793259013	No Hit
AGGCCTGGTTTGCGCTGGAGAGTGGTCTTGGTTGTGAGGGTCATAAGAT	5943	0.26182739041402475	No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA	5930	0.2612546567651299	No Hit
CTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAATCCT	5422	0.23887398802369883	No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCC	5391	0.23750823855325717	No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA	5181	0.22825638730187822	Illumina Single End Adapter 1 (100% over 32bp)
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAAG	5155	0.22711092000408845	No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA	4969	0.2189164231814385	Illumina Single End Adapter 1 (100% over 32bp)
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGAC	4914	0.21649331928226778	No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAAT	4700	0.20706524229276735	No Hit
ATCCGAATTCAGTCTTCCTGAGGCTCTGGCTCACCTCCAGGCAGGTCCCA	4444	0.19578679505299107	No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCCG	4248	0.1871517338850374	No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC	4108	0.18098383305078472	No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG	3955	0.1742431985676372	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAAT	3617	0.15935212369637008	No Hit
AAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCGAATG	3563	0.1569730762317298	No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGT	3430	0.1511135704391898	No Hit
CTGCGCGTCTGCTCCTCCTTCTCACGTTTCTCCTGCCGCCCTAATCCT	3320	0.14626736264084841	No Hit
AACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTT	3269	0.14402048447979926	No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGGTTGTGAGGGTTCCTGCTT	3265	0.14384425874167775	No Hit
AGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTCGCT	3123	0.13758824503836434	No Hit
CAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGG	3052	0.13446023818670766	No Hit
CTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAATCCTG	2894	0.12749932153090823	No Hit
CTCTGCAGGTACCATGGAGCTGAGCTATAGGCTCTTCATCTGCCTCCT	2769	0.12199226721461122	No Hit
CGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTG	2750	0.12115519495853408	No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTG	2719	0.11978944548809242	No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTAG	2707	0.1192607682737279	No Hit
TCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGC	2690	0.11851180888671152	No Hit
TCTGGAAGAGGTCTTGCGAAAGCCGCCTCGAGCGCGCTCCGCGGCTGC	2644	0.11648521289831422	No Hit
ATCAGATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGG	2530	0.11146277936185135	No Hit
CGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTC	2491	0.10974457841516669	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.22904940312342498	0.0	0.0	0.0	0.0
2	0.3514381782488096	0.0	0.0	0.0	0.0
3	0.40721362436426567	0.0	0.0	0.0	0.0
4	0.4751486464101055	0.0	0.0	0.0	0.0
5	0.5555516394280418	0.0	0.0	0.0	0.0
6	0.6434882827506723	0.0	0.0	0.0	0.0
7	0.7060924762183366	0.0	0.0	0.0	0.0
8	0.770811378543459	0.0	0.0	0.0	0.0
9	0.8204189238246624	0.0	0.0	0.0	0.0
10	0.8646956405276903	0.0	0.0	0.0	0.0
11	0.9116157433025408	0.0	0.0	0.0	0.0
12	0.9453629721528088	0.0	0.0	0.0	0.0
13	0.9803437811699274	0.0	0.0	0.0	0.0
14	1.0145315743654992	0.0	0.0	0.0	0.0
15	1.0520676565853797	0.0	0.0	0.0	0.0
16	1.080131605381229	0.0	0.0	0.0	0.0
17	1.1197823964585676	0.0	0.0	0.0	0.0
18	1.142559573110772	0.0	0.0	0.0	0.0
19	1.1812851790629726	0.0	0.0	0.0	0.0
20	1.2008462359944594	0.0	0.0	0.0	0.0
21	1.2268835888019116	0.0	0.0	0.0	0.0
22	1.244726444786714	0.0	0.0	0.0	0.0
23	1.2627014700751074	0.0	0.0	0.0	0.0
24	1.2816897933576994	0.0	0.0	0.0	0.0
25	1.2988277463900157	0.0	0.0	0.0	0.0
26	1.3256140585844844	0.0	0.0	0.0	0.0
27	1.3429722937894526	0.0	0.0	0.0	0.0
28	1.3578633686607195	0.0	0.0	0.0	0.0
29	1.3775125384612674	0.0	0.0	0.0	0.0
30	1.3931085162850205	0.0	0.0	0.0	0.0
31	1.4072065753347407	0.0	0.0	0.0	0.0
32	1.4202032235212017	0.0	0.0	0.0	0.0
33	1.4324509123206464	0.0	0.0	0.0	0.0
34	1.447650382233626	0.0	0.0	0.0	0.0
35	1.4618806105869375	0.0	0.0	0.0	0.0
36	1.4761548953747792	0.0	0.0	0.0	0.0
37	1.4882704148706327	0.0	0.0	0.0	0.0
38	1.498271225509028	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAAGCCA	5460	0.0	107.91729	44
ATTAGTC	600	0.0	87.140785	44
CTATACG	135	0.0	78.328804	44
CGACGGG	3105	0.0	71.895996	44
TTAAGCC	5395	0.0	60.592274	43
CAATGAA	3540	0.0	56.981686	43
GGTCCCA	1255	0.0	55.703945	44
AGGTCCC	2485	0.0	54.811428	43
AATGAAG	1285	0.0	48.460228	44
CCAGAAT	1030	0.0	47.90985	44
ATTAAGC	5415	0.0	47.042957	42
TATTAGT	625	0.0	46.699337	43
ACGCTAC	445	0.0	46.64103	43
ACTACAC	345	0.0	45.975597	44
ATAAGAT	1090	0.0	44.033524	43
TAATCCT	2340	0.0	43.868244	42
GCAATGA	3630	0.0	43.56449	42
AGATTAA	5535	0.0	43.44756	40
GATTAAG	5730	0.0	43.439392	41
TCGTGCG	910	0.0	42.764954	43
>>END_MODULE