FastQCFastQC Report
Tue 24 May 2016
ERR841166.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841166.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences945837
Sequences flagged as poor quality0
Sequence length24-50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA109451.1571761307709467No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTAGGACTGGGAAGCAGTGGT100001.0572646238199606No Hit
AAGCAGTGGTATCAACGCAGAGTGCAG72130.7626049731613375No Hit
AAGCAGTGGTATCAACGCAGAGTG65640.6939884990754221No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAAG47830.5056896695730871No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC47690.5042094990997391No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA46630.4930024940872476No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGC38450.40651824785877483No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA33360.3527034785063389No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTG32550.3441396350533971No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGAC26260.27763769021512164No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCT26250.27753196375273964No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA22160.23428984063850322Illumina Single End Adapter 1 (100% over 32bp)
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA22030.23291539662753732Illumina Single End Adapter 1 (100% over 32bp)
AAGCAGTGGTATCAACGCAGAGTGCAGT21540.2277347999708195No Hit
TCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA21100.22308283562601167Illumina Single End Adapter 1 (100% over 31bp)
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAAT19890.21028993367779017No Hit
AAGCAGTGGTATCAACGCAGAGTGCA19500.2061666016448923No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTAG18730.1980256640414786No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTA18100.19136489691141287No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTAGG17690.18703011195375102No Hit
TCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA17100.18079225067321325Illumina Single End Adapter 1 (100% over 31bp)
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCCG17080.18058079774844926No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC16150.17074823674692363No Hit
AGGCCTGGTTTGCGCTGGAGAGTGGTCTTGGTTGTGAGGGTCATAAGATG15820.16725926348831777No Hit
CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGG15430.1631359314554199No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTAGGACTGGG14550.15383200276580428No Hit
AGGCCTGGTTTGCGCTGGAGAGTGGTCTTGGTTGTGAGGGTCATAAGAT13920.1471712356357385No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCC13720.1450567063880986No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTAGGAC13640.1442108946890426No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGA13120.13871311864517882No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTAG12470.1318408985903491No Hit
AAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTCTTCTGCT12400.1311008133536751Illumina Single End Adapter 2 (100% over 32bp)
AAGCAGTGGTATCAACGCAGAGTGC12230.12930346349318117No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGC12090.12782329301983322No Hit
ATCCGAATTCAGTCTTCCTGAGGCTCTGGCTCACCTCCAGGCAGGTCCC11550.12211406405120544No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTAGGACTGGGAAGCAGTGG11450.12105679942738547No Hit
CTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAATCCTG11220.11862509079259957No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATC11180.11820218494307158No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG10890.1151361175339937No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA10730.11344449413588176Illumina Single End Adapter 1 (100% over 32bp)
AAGCAGTGGTATCAACGCAGAGTGCAGG10610.1121757765872978No Hit
CTTCCGCCACGGCCGTCTCTGGAGAGCAGCAGCCATGGCCCTACGCTAC10470.11069560611394987No Hit
AAGCAGTGGTATCAACAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG10320.10910970917821994Illumina Single End Adapter 2 (100% over 34bp)
CTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAATCCT10260.10847535040392794No Hit
CCCTTCCACCTTCTGCCGTGTTAGGATTTGGTGTTCCTCCCTTCCAGAA9590.1013916774243342No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGCCA13350.075.68841644
ATTAGTC6350.074.0751944
CGGGTAT200.002924186665.330244
AATGAAG9400.053.7468344
GATACTC507.709423E-752.2641644
TTAAGCC13150.051.69782643
AATCCCG2550.047.64740843
ATAAGAT4250.046.2860543
CAATGAA13500.046.07176643
CCAGAAT5500.045.92910844
TATTAGT7150.045.71961243
ATTAAGC13500.044.80832342
GATTAAG13900.043.32526841
AGATTAA13550.042.69696840
ATATTAG6900.042.5285342
GCAATGA13550.041.983442
CTAATCC9950.041.18696241
AAGCAAT13350.040.93927440
TAATCCT5900.040.57472242
ATATGCT13450.040.45554725
Produced by FastQC (version 0.11.5)