##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841166.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	945837
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.39969149018277	39.0	37.0	39.0	34.0	39.0
2	37.98032324808609	39.0	38.0	39.0	35.0	39.0
3	38.16692833966106	39.0	38.0	39.0	35.0	39.0
4	38.36338713753004	39.0	38.0	40.0	35.0	41.0
5	39.32441319170216	40.0	39.0	41.0	36.0	41.0
6	39.216649380390066	40.0	39.0	41.0	36.0	41.0
7	38.92870547462195	40.0	38.0	41.0	35.0	41.0
8	39.15706723251469	40.0	38.0	41.0	35.0	41.0
9	39.22951523359733	40.0	39.0	41.0	36.0	41.0
10	38.79665629490071	40.0	38.0	41.0	35.0	41.0
11	39.06424257033717	40.0	39.0	41.0	35.0	41.0
12	39.134090757709835	40.0	39.0	41.0	35.0	41.0
13	39.16464676260286	40.0	39.0	41.0	35.0	41.0
14	39.12392198655794	40.0	39.0	41.0	35.0	41.0
15	39.15696573511081	40.0	38.0	41.0	35.0	41.0
16	39.072527295929426	40.0	38.0	41.0	35.0	41.0
17	38.93798720075446	40.0	38.0	41.0	35.0	41.0
18	38.91147629031218	40.0	38.0	41.0	35.0	41.0
19	38.86846147909206	40.0	38.0	41.0	35.0	41.0
20	38.81010152912182	40.0	38.0	41.0	35.0	41.0
21	38.48381486450625	40.0	38.0	41.0	34.0	41.0
22	38.46976276039106	40.0	38.0	41.0	34.0	41.0
23	38.048273645459	40.0	37.0	41.0	33.0	41.0
24	38.0781054240847	40.0	37.0	41.0	33.0	41.0
25	38.11413201091976	40.0	37.0	41.0	33.0	41.0
26	38.13464178097013	40.0	37.0	41.0	33.0	41.0
27	38.093479319949175	40.0	37.0	41.0	33.0	41.0
28	38.01530810791742	40.0	37.0	41.0	33.0	41.0
29	38.16886819711352	40.0	37.0	41.0	33.0	41.0
30	38.3415045884324	40.0	37.0	41.0	34.0	41.0
31	38.31678078187626	40.0	37.0	41.0	34.0	41.0
32	38.36089838387528	40.0	37.0	41.0	34.0	41.0
33	38.340017212520095	40.0	37.0	41.0	34.0	41.0
34	38.31281923374355	40.0	37.0	41.0	34.0	41.0
35	38.326537943529544	40.0	37.0	41.0	34.0	41.0
36	38.225275773009244	40.0	36.0	41.0	34.0	41.0
37	38.16359261839962	40.0	36.0	41.0	34.0	41.0
38	38.11488348006215	40.0	36.0	41.0	34.0	41.0
39	37.99727653178257	40.0	35.0	41.0	34.0	41.0
40	37.83569422185459	39.0	35.0	41.0	34.0	41.0
41	37.69090000632314	39.0	35.0	41.0	34.0	41.0
42	37.62925032772934	39.0	35.0	41.0	34.0	41.0
43	37.537260791493914	39.0	35.0	41.0	34.0	41.0
44	37.46239681451977	39.0	35.0	41.0	33.0	41.0
45	37.39654483313435	38.0	35.0	41.0	34.0	41.0
46	37.275176062931926	38.0	35.0	41.0	33.0	41.0
47	37.045769824160686	37.0	35.0	41.0	33.0	41.0
48	37.05201006176717	37.0	35.0	40.0	33.0	41.0
49	37.08095248187868	38.0	35.0	40.0	33.0	41.0
50	36.856236704874895	38.0	35.0	40.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	30.0
15	260.0
16	359.0
17	16.0
18	0.0
19	0.0
20	0.0
21	1.0
22	5.0
23	9.0
24	29.0
25	91.0
26	244.0
27	713.0
28	1682.0
29	3616.0
30	6817.0
31	11455.0
32	17253.0
33	24853.0
34	44507.0
35	44991.0
36	65282.0
37	97937.0
38	149922.0
39	223226.0
40	252539.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	52.60948768128123	14.624295729602457	32.766216589116304
2	26.337519043979036	28.501951181863262	26.865834176501867	18.29469559765583
3	36.03443299426857	17.70114723784331	20.19819482638129	26.06622494150683
4	25.581046205635854	16.068730658665288	25.04152406810053	33.30869906759833
5	24.31074276011617	28.793967670962335	23.93763407437011	22.95765549455139
6	32.02623707890472	25.30425432711979	21.1371515387958	21.5323570551797
7	29.066424764520736	20.40594732496191	26.900935362012696	23.626692548504657
8	34.56398935545977	19.2602953785906	24.96857280905695	21.207142456892676
9	34.86414678216225	19.550620244291565	22.89760286391841	22.68763010962777
10	29.41775379901611	18.88834968393074	30.526718662940866	21.167177854112285
11	28.768910499377796	27.360739746911992	23.25865873295293	20.611691020757274
12	26.65438125173788	19.04355613070751	31.783594847738033	22.518467769816574
13	24.16209135400709	17.96704929073403	24.455482287117125	33.41537706814176
14	22.72421146561194	28.27263048495671	22.32911167569042	26.67404637374093
15	21.115160434620343	30.449432618939625	23.733476275510473	24.70193067092956
16	25.568993388924305	24.26295439911951	22.64333072188971	27.524721490066472
17	33.748309698182666	22.985673007082617	19.030657502296908	24.23535979243781
18	24.533402689892654	20.696483643587637	20.918614941052212	33.85149872546749
19	24.669578373440668	27.9978474092259	23.094676989798455	24.237897227534976
20	34.89195284176872	19.150868490025236	22.0960905526005	23.861088115605543
21	25.275391002889503	27.178678778690195	23.96892910723518	23.57700111118512
22	32.33210373457583	21.93718367964036	23.254641127382413	22.476071458401396
23	26.256321120869664	19.680029434247125	29.046759642517685	25.016889802365522
24	32.50866692675377	19.799606063201164	21.887809421707967	25.803917588337104
25	24.322036528321917	22.544633063407147	24.47570931871373	28.657621089557207
26	25.135888434222892	26.796477935421635	24.313937002025003	23.753696628330463
27	31.19395163171618	21.5019579292064	23.428529887580467	23.875560551496953
28	26.174593567860686	20.37082970915036	28.69096164594681	24.763615077042136
29	32.03100318508933	20.641582448617527	24.182281999017377	23.145132367275764
30	24.925709714259213	21.19826781604531	23.885703685238756	29.990318784456722
31	26.255267305524765	20.851748441438787	30.915751172720853	21.977233080315596
32	26.813058617662485	24.894212470614573	24.779793432983556	23.51293547873939
33	29.331343382663245	22.466724832023825	23.53871394498632	24.663217840326613
34	25.73847417011161	24.677066725222687	26.72684570806641	22.857613396599298
35	24.922837003156204	26.325046946580017	24.02883955743462	24.723276492829157
36	24.695744526780757	23.624634917569818	25.802457215959834	25.87716333968959
37	23.8669884362049	24.453675626200262	25.544382102945214	26.134953834649625
38	28.258323940658602	25.964236756328628	22.974746397782315	22.80269290523045
39	27.452192422515797	26.71863084826549	21.941116781093132	23.88805994812558
40	26.438012520833183	26.917575694911168	22.228787084290367	24.41562469996529
41	25.20764083559706	27.60696339148558	22.609945187034832	24.57545058588253
42	25.401554355582274	28.108492470035728	23.71898130849297	22.77097186588903
43	24.499011389199932	25.644236298156947	25.636039907595155	24.220712405047966
44	23.657555789765425	26.068405950399775	24.172831626977416	26.101206632857384
45	24.444037364788347	27.538853333469977	24.86382758497005	23.153281716771627
46	26.115172860026583	26.175356484656405	24.39309463939548	23.316376015921538
47	28.9036040118501	23.630389057257137	23.93515450442545	23.530852426467312
48	25.894356836852168	26.306452526091306	22.63142711554586	25.167763521510665
49	25.988118955776113	25.52971169392686	22.782295911879228	25.699873438417796
50	24.290264506301064	26.76732935132276	25.925976375825517	23.016429766550658
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	889.0
1	948.5
2	1068.0
3	1076.5
4	1010.0
5	879.5
6	713.5
7	617.0
8	562.5
9	594.0
10	638.0
11	605.0
12	588.0
13	661.0
14	719.5
15	831.0
16	962.5
17	1105.0
18	1239.0
19	1645.5
20	2076.5
21	2473.5
22	2921.0
23	3995.5
24	5025.0
25	5485.5
26	6157.5
27	7296.0
28	8363.5
29	9273.0
30	10461.5
31	11977.5
32	13432.5
33	14816.5
34	16445.0
35	18546.5
36	20861.0
37	23550.5
38	28285.0
39	34802.5
40	40688.0
41	45504.0
42	50845.5
43	60906.0
44	64657.0
45	54592.5
46	48448.5
47	50059.0
48	59181.5
49	69182.0
50	84624.5
51	104083.0
52	107377.5
53	104162.5
54	96516.5
55	76446.5
56	60166.5
57	54777.0
58	54215.5
59	54639.0
60	51839.0
61	47752.0
62	44058.5
63	41128.0
64	39959.5
65	39173.0
66	35999.5
67	32422.5
68	28324.0
69	24185.0
70	21120.5
71	18046.5
72	15759.5
73	13949.5
74	11680.5
75	9703.5
76	8381.0
77	6453.0
78	4850.0
79	4214.5
80	3413.5
81	2306.0
82	1451.5
83	1222.0
84	996.5
85	773.0
86	601.0
87	417.0
88	236.0
89	155.0
90	107.5
91	64.0
92	38.0
93	28.5
94	17.5
95	6.5
96	5.0
97	5.0
98	8.5
99	12.0
100	12.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	11384.0
25	5566.0
26	7250.0
27	14536.0
28	17644.0
29	26962.0
30	7219.0
31	6641.0
32	5063.0
33	5628.0
34	5615.0
35	3749.0
36	4802.0
37	3103.0
38	3702.0
39	4567.0
40	5845.0
41	6358.0
42	7171.0
43	6463.0
44	5941.0
45	6331.0
46	10759.0
47	21720.0
48	81270.0
49	220067.0
50	440481.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.399596097824958
#Duplication Level	Percentage of deduplicated	Percentage of total
1	56.29719786147953	10.921428999520378
2	9.624130895685152	3.7340850433778066
3	5.525645608048748	3.2158587892759836
4	4.310686897926683	3.3450233889585457
5	3.510998015675156	3.4055971702181465
6	3.0458274815571826	3.545269375551884
7	2.579504209909563	3.502893786341923
8	2.1222888064031857	3.293723651772547
9	1.7877485603383505	3.121343999552885
>10	10.621343388462359	34.78051628303448
>50	0.318927085179516	4.2595171324935475
>100	0.2092609295648901	7.921169212848625
>500	0.0212618056777161	2.6296076155238817
>1k	0.022940369283851583	8.55507115599481
>5k	0.0016785636061354818	2.580870976401855
>10k+	5.595212020451605E-4	1.188023419132704
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA	10945	1.1571761307709467	No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTAGGACTGGGAAGCAGTGGT	10000	1.0572646238199606	No Hit
AAGCAGTGGTATCAACGCAGAGTGCAG	7213	0.7626049731613375	No Hit
AAGCAGTGGTATCAACGCAGAGTG	6564	0.6939884990754221	No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAAG	4783	0.5056896695730871	No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC	4769	0.5042094990997391	No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA	4663	0.4930024940872476	No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGC	3845	0.40651824785877483	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA	3336	0.3527034785063389	No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTG	3255	0.3441396350533971	No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGAC	2626	0.27763769021512164	No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCT	2625	0.27753196375273964	No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA	2216	0.23428984063850322	Illumina Single End Adapter 1 (100% over 32bp)
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA	2203	0.23291539662753732	Illumina Single End Adapter 1 (100% over 32bp)
AAGCAGTGGTATCAACGCAGAGTGCAGT	2154	0.2277347999708195	No Hit
TCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA	2110	0.22308283562601167	Illumina Single End Adapter 1 (100% over 31bp)
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAAT	1989	0.21028993367779017	No Hit
AAGCAGTGGTATCAACGCAGAGTGCA	1950	0.2061666016448923	No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTAG	1873	0.1980256640414786	No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTA	1810	0.19136489691141287	No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTAGG	1769	0.18703011195375102	No Hit
TCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA	1710	0.18079225067321325	Illumina Single End Adapter 1 (100% over 31bp)
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCCG	1708	0.18058079774844926	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC	1615	0.17074823674692363	No Hit
AGGCCTGGTTTGCGCTGGAGAGTGGTCTTGGTTGTGAGGGTCATAAGATG	1582	0.16725926348831777	No Hit
CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGG	1543	0.1631359314554199	No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTAGGACTGGG	1455	0.15383200276580428	No Hit
AGGCCTGGTTTGCGCTGGAGAGTGGTCTTGGTTGTGAGGGTCATAAGAT	1392	0.1471712356357385	No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCC	1372	0.1450567063880986	No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTAGGAC	1364	0.1442108946890426	No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGA	1312	0.13871311864517882	No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTAG	1247	0.1318408985903491	No Hit
AAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTCTTCTGCT	1240	0.1311008133536751	Illumina Single End Adapter 2 (100% over 32bp)
AAGCAGTGGTATCAACGCAGAGTGC	1223	0.12930346349318117	No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGC	1209	0.12782329301983322	No Hit
ATCCGAATTCAGTCTTCCTGAGGCTCTGGCTCACCTCCAGGCAGGTCCC	1155	0.12211406405120544	No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTAGGACTGGGAAGCAGTGG	1145	0.12105679942738547	No Hit
CTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAATCCTG	1122	0.11862509079259957	No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATC	1118	0.11820218494307158	No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG	1089	0.1151361175339937	No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA	1073	0.11344449413588176	Illumina Single End Adapter 1 (100% over 32bp)
AAGCAGTGGTATCAACGCAGAGTGCAGG	1061	0.1121757765872978	No Hit
CTTCCGCCACGGCCGTCTCTGGAGAGCAGCAGCCATGGCCCTACGCTAC	1047	0.11069560611394987	No Hit
AAGCAGTGGTATCAACAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG	1032	0.10910970917821994	Illumina Single End Adapter 2 (100% over 34bp)
CTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAATCCT	1026	0.10847535040392794	No Hit
CCCTTCCACCTTCTGCCGTGTTAGGATTTGGTGTTCCTCCCTTCCAGAA	959	0.1013916774243342	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.241479240080479	0.0	0.0	0.0	0.0
2	0.43739037487431764	0.0	0.0	0.0	0.0
3	0.5468172634396836	0.0	0.0	0.0	0.0
4	0.643134070669682	0.0	0.0	0.0	0.0
5	0.8053184639636639	0.0	0.0	0.0	0.0
6	0.938322353640215	0.0	0.0	0.0	0.0
7	1.0543042828732647	0.0	0.0	0.0	0.0
8	1.2209291875872905	0.0	0.0	0.0	0.0
9	1.3031843753204833	0.0	0.0	0.0	0.0
10	1.3890342627746641	0.0	0.0	0.0	0.0
11	1.5123113179120715	0.0	0.0	0.0	0.0
12	1.575535742416505	0.0	0.0	0.0	0.0
13	1.6554649479772943	0.0	0.0	0.0	0.0
14	1.7448038086900808	0.0	0.0	0.0	0.0
15	1.8511646298463689	0.0	0.0	0.0	0.0
16	1.9159749512865325	0.0	0.0	0.0	0.0
17	2.089894981904916	0.0	0.0	0.0	0.0
18	2.15100487716171	0.0	0.0	0.0	0.0
19	2.3414182359116844	0.0	0.0	0.0	0.0
20	2.382228650391135	0.0	0.0	0.0	0.0
21	2.469770161243428	0.0	0.0	0.0	0.0
22	2.5011709205708805	0.0	0.0	0.0	0.0
23	2.5559372280847548	0.0	0.0	0.0	0.0
24	2.6292056665154777	0.0	0.0	0.0	0.0
25	2.6873552208255758	0.0	0.0	0.0	0.0
26	2.8707906330583386	0.0	0.0	0.0	0.0
27	2.9018742129986457	0.0	0.0	0.0	0.0
28	2.9546317177272616	0.0	0.0	0.0	0.0
29	3.0084464870796976	0.0	0.0	0.0	0.0
30	3.0449221166014864	0.0	0.0	0.0	0.0
31	3.0688162970998176	0.0	0.0	0.0	0.0
32	3.089750136651453	0.0	0.0	0.0	0.0
33	3.1078293617187738	0.0	0.0	0.0	0.0
34	3.1277059366465894	0.0	0.0	0.0	0.0
35	3.1514943906825383	0.0	0.0	0.0	0.0
36	3.1677762658893656	0.0	0.0	0.0	0.0
37	3.1961109578077407	0.0	0.0	0.0	0.0
38	3.211864200702658	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAAGCCA	1335	0.0	75.688416	44
ATTAGTC	635	0.0	74.07519	44
CGGGTAT	20	0.0029241866	65.3302	44
AATGAAG	940	0.0	53.74683	44
GATACTC	50	7.709423E-7	52.26416	44
TTAAGCC	1315	0.0	51.697826	43
AATCCCG	255	0.0	47.647408	43
ATAAGAT	425	0.0	46.28605	43
CAATGAA	1350	0.0	46.071766	43
CCAGAAT	550	0.0	45.929108	44
TATTAGT	715	0.0	45.719612	43
ATTAAGC	1350	0.0	44.808323	42
GATTAAG	1390	0.0	43.325268	41
AGATTAA	1355	0.0	42.696968	40
ATATTAG	690	0.0	42.52853	42
GCAATGA	1355	0.0	41.9834	42
CTAATCC	995	0.0	41.186962	41
AAGCAAT	1335	0.0	40.939274	40
TAATCCT	590	0.0	40.574722	42
ATATGCT	1345	0.0	40.455547	25
>>END_MODULE