FastQCFastQC Report
Tue 24 May 2016
ERR841165.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841165.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences907547
Sequences flagged as poor quality0
Sequence length24-50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA394174.3432461349109195No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC269002.9640338186341864No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGC56120.6183701780734221No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCC40350.444605072795128No Hit
CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGG40280.4438337628794983No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA38300.4220167109802578No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGC37720.4156258573936116No Hit
CAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGCGC33860.3730936249031731No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA31110.34279216393200573No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGA28330.31216014156842564No Hit
CGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGT27380.30169236414202244No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCC26670.293869077854921No Hit
ATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGC25170.27734100823428426No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGT24700.2721622130864848No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCG22800.25122665823367824No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG19730.2173992090767751No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC18180.20032020380211712No Hit
ATCAGATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGG17920.19745533840120677No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA17760.19569234430833887Illumina Single End Adapter 1 (100% over 32bp)
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA17510.19293766603823273Illumina Single End Adapter 1 (100% over 32bp)
TACCTGGTTGATCCTGCCAGTAGCATATG16930.18654681245158655No Hit
AGGCCTGGTTTGCGCTGGAGAGTGGTCTTGGTTGTGAGGGTCATAAGAT15870.17486697658633657No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTGCAGGAGGGAAGCAGTGGT15370.16935762004612434No Hit
TGGAGAAGGGTTCCATGTGAACAGCAGTTGAACATGGGTCAGTCGGTCC15330.16891687152290735No Hit
AAGCAGTGGTATCAACGCAGAGTG15230.1678150002148649No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA14860.16373807637510784No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGC14700.16197508228223992No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGAC14340.1580083455732871No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGT13970.15393142173353005No Hit
CGCGACCTCAGATCAGACGTGGCGACCCG13630.15018505928618572No Hit
AAGCAGTGGTATCAACGCAGAGTGCAG13560.14941374937055601No Hit
CTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAATCCT13170.14511645126919046No Hit
AACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTT12580.13861541055174004No Hit
ATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAAT11980.13200418270348532No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCC11640.12825782025614102No Hit
TCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA11540.12715594894809856Illumina Single End Adapter 1 (100% over 31bp)
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAAT10920.12032434683823538No Hit
CGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGGGGAATCA10700.11790022996054199No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCT10130.11161956350470004No Hit
ACACCCGGGTGAGGGGTCTGCTGGCTGCACCTGTCGGTCTCATGGCTCC9910.10919544662700664No Hit
CTTCCGCCACGGCCGTCTCTGGAGAGCAGCAGCCATGGCCCTACGCTAC9790.1078732010573557No Hit
AGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTCGCT9640.10622039409529202No Hit
TCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGC9390.1034657158251859No Hit
CAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGG9340.10291478017116469No Hit
CCCTGTAATTGGAATGAGTCCACTTTAAATCCTTTAACGAGGATCCAT9290.10236384451714345No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTAGTC30350.089.968344
TAAGCCA44100.088.8373844
TTAAGCC47650.057.40008543
TATTAGT33450.057.08278743
ATATTAG33600.047.7705342
ATTAAGC47800.047.70683742
CATATTA33550.045.9021641
AACGACA401.9948602E-645.53463743
GATTAAG49100.045.52337641
AGATTAA48650.044.7800240
GCATATT33900.044.6901140
AAGCATA33650.044.06888638
TAAGCAT33600.043.950137
CAATGAA6900.043.5548743
AAGATTA50500.042.93227839
CAAAGAT49800.042.9152437
GTCTCAA48900.042.8493133
TTAAGCA34400.042.8203136
AAAGATT50750.042.51449638
CTCAAAG49700.042.48272735
Produced by FastQC (version 0.11.5)