##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841165.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	907547
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.671498005062	39.0	35.0	39.0	32.0	39.0
2	37.578494557306676	39.0	37.0	39.0	35.0	39.0
3	37.92293842632944	39.0	37.0	39.0	35.0	39.0
4	38.18958467164786	39.0	38.0	40.0	35.0	41.0
5	38.93250928051109	40.0	38.0	41.0	35.0	41.0
6	38.88503625707539	40.0	38.0	41.0	35.0	41.0
7	38.8226031268904	40.0	38.0	41.0	35.0	41.0
8	38.7860639724444	40.0	38.0	41.0	35.0	41.0
9	38.82955703671546	40.0	38.0	41.0	35.0	41.0
10	38.7367960006479	40.0	38.0	41.0	35.0	41.0
11	38.82829980155298	40.0	38.0	41.0	35.0	41.0
12	38.82028699340089	40.0	38.0	41.0	35.0	41.0
13	38.840662797629214	40.0	38.0	41.0	35.0	41.0
14	38.83169025956782	40.0	38.0	41.0	35.0	41.0
15	38.81737915501897	40.0	38.0	41.0	35.0	41.0
16	38.74869621077476	40.0	38.0	41.0	35.0	41.0
17	38.67525428435111	40.0	38.0	41.0	35.0	41.0
18	38.65755933301526	40.0	38.0	41.0	35.0	41.0
19	38.528423321326606	40.0	38.0	41.0	35.0	41.0
20	38.4056638388976	40.0	37.0	41.0	34.0	41.0
21	38.22939748575005	40.0	37.0	41.0	34.0	41.0
22	38.16173156872316	40.0	37.0	41.0	34.0	41.0
23	37.97743918496783	40.0	36.0	41.0	33.0	41.0
24	37.953397454897654	40.0	36.0	41.0	33.0	41.0
25	37.8662704061947	40.0	36.0	41.0	33.0	41.0
26	37.755711579306116	39.0	36.0	41.0	33.0	41.0
27	37.75313844105603	39.0	36.0	40.0	33.0	41.0
28	37.85623997778823	39.0	36.0	41.0	34.0	41.0
29	37.87796673002598	39.0	36.0	41.0	34.0	41.0
30	37.913792513495174	39.0	35.0	41.0	34.0	41.0
31	37.890823762766196	40.0	35.0	41.0	34.0	41.0
32	37.893179589409826	39.0	35.0	41.0	34.0	41.0
33	37.84335642581093	39.0	35.0	41.0	34.0	41.0
34	37.78926645286056	39.0	35.0	41.0	34.0	41.0
35	37.689331361546294	39.0	35.0	41.0	34.0	41.0
36	37.62863743800372	39.0	35.0	41.0	34.0	41.0
37	37.53751662367406	39.0	35.0	41.0	34.0	41.0
38	37.40560905471799	38.0	35.0	41.0	34.0	41.0
39	37.31674081837036	38.0	35.0	41.0	34.0	41.0
40	37.12632158766342	37.0	35.0	41.0	33.0	41.0
41	36.988063491749806	37.0	35.0	41.0	33.0	41.0
42	36.90973339890319	37.0	35.0	41.0	33.0	41.0
43	36.80976429502945	37.0	35.0	40.0	33.0	41.0
44	36.72687019401508	36.0	35.0	40.0	33.0	41.0
45	36.60689935064935	36.0	35.0	40.0	33.0	41.0
46	36.57096105164022	36.0	35.0	40.0	33.0	41.0
47	36.458880083317574	36.0	35.0	40.0	33.0	41.0
48	36.39342602518706	36.0	35.0	40.0	33.0	41.0
49	36.5104079489458	36.0	35.0	40.0	33.0	41.0
50	36.616332127156156	37.0	35.0	40.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	44.0
15	278.0
16	105.0
17	0.0
18	0.0
19	0.0
20	1.0
21	0.0
22	14.0
23	30.0
24	77.0
25	179.0
26	393.0
27	779.0
28	1786.0
29	3488.0
30	6366.0
31	10670.0
32	17191.0
33	29843.0
34	66045.0
35	51490.0
36	71072.0
37	120813.0
38	159119.0
39	189990.0
40	177773.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	37.51199662386631	31.97696648217668	30.511036893957005
2	33.44774430415174	26.953535188811156	21.483846015688442	18.114874491348658
3	27.654104966464548	16.584815993000916	20.376465351105782	35.384613689428754
4	23.55624557185468	18.28775809958052	26.018266822544728	32.13772950602007
5	23.346449274803398	25.66247257717782	29.468336075156436	21.522742072862343
6	31.1805339007236	23.079355669733907	20.478278259968903	25.26183216957359
7	33.09602698262459	20.720579760607443	21.066457164201964	25.116936092566007
8	28.89293887809667	19.295529597916143	29.50965624920803	22.301875274779157
9	24.833865353529898	22.20612265811027	27.099312762865175	25.86069922549466
10	30.0903424285464	22.82504377183771	23.502143690629794	23.582470108986094
11	29.651797647945504	24.703624164919283	23.55988174717122	22.08469643996399
12	24.616466144453124	21.89616625915793	29.206090703842335	24.281276892546614
13	22.661085321200996	17.885575072145024	28.01529838124086	31.438041225413123
14	21.912804515909368	22.3523409806875	21.494534167376457	34.24032033602668
15	22.303858643133633	26.66517546749645	27.909298361407174	23.12166752796274
16	32.94958828578575	23.81143896679731	20.505163919885142	22.7338088275318
17	26.29142071980845	25.036499487078906	19.795999546029023	28.876080247083625
18	25.055892422100452	20.44158594541109	20.120831207639934	34.38169042484852
19	26.56215050019448	27.071765980164113	22.941181007705385	23.424902511936022
20	33.17833676933537	17.590934684374474	23.905979525027355	25.32474902126281
21	28.786057361216557	20.485440423471182	26.043058926975682	24.685443288336582
22	28.313134195804736	24.90339343306738	23.79942856953965	22.98404380158824
23	29.64144005764991	19.88734467746574	22.140891876674157	28.3303233882102
24	31.316284445874427	19.992022451729774	20.845862528331864	27.845830574063935
25	23.5631954515418	28.813813014728385	23.918543748309183	23.70444778542063
26	25.343006607868183	20.498870102431425	26.797895906863918	27.36022738283647
27	24.987168309197802	25.490910231956626	21.817236378795997	27.70468508004958
28	23.158151210031562	19.21771502071021	28.467557781769347	29.156575987488882
29	36.51407854790114	19.888416987972473	22.138843253038544	21.458661211087843
30	23.982183416418394	21.83097843721424	21.991917642547417	32.194920503819944
31	23.4638157231339	20.643479024235155	34.048916363860684	21.84378888877026
32	28.178773186405177	19.763807580987834	27.039039310988272	25.01837992161872
33	29.21006975603336	23.87247687760987	22.09351855175467	24.823934814602104
34	20.907715037295954	26.967556724682595	29.78883643832655	22.335891799694906
35	24.513406061073933	22.142195438513536	25.86100831335009	27.48339018706244
36	23.829820210787354	22.110513212957223	30.914590437073773	23.14507613918165
37	23.111222408347942	22.29420073861385	25.545754915380332	29.04882193765788
38	25.133991915953242	31.247415020515128	20.75352685755558	22.865066205976046
39	23.938104344547096	32.6822919854945	20.040303919960234	23.339299749998162
40	27.133813094706767	29.179499853071544	20.134362693204775	23.552324359016918
41	28.387126258465422	22.950234493593886	20.399398728040545	28.26324051990015
42	24.95231282784931	31.690982157049753	21.558327372436814	21.798377642664125
43	22.245492772306072	22.25574570720869	32.36914066651579	23.12962085396945
44	23.660998763320748	25.69648213867431	26.43357823444517	24.20894086355977
45	24.667588271103895	26.970119724025977	27.84369926948052	20.51859273538961
46	25.565811214377632	28.618271827067698	24.622611491420983	21.193305467133687
47	33.42962178070587	24.497076371851303	19.721212560849548	22.35208928659328
48	26.860604879316508	23.444217046205157	19.90655015214025	29.788627922338083
49	23.74666774375959	22.14912351563131	25.139833589142903	28.964375151466193
50	21.66270639896772	31.47137622879826	19.586785415777605	27.279131956456414
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	994.0
1	983.5
2	1109.5
3	1218.0
4	1124.5
5	859.5
6	597.0
7	569.5
8	625.0
9	663.0
10	687.5
11	696.0
12	704.5
13	744.5
14	772.5
15	826.5
16	915.5
17	1171.5
18	1412.0
19	1793.0
20	2245.5
21	2698.0
22	3090.5
23	3699.5
24	4295.0
25	4731.5
26	5504.0
27	6679.5
28	7787.5
29	8820.5
30	9866.0
31	10866.5
32	12212.0
33	14107.5
34	16247.0
35	18268.0
36	20218.5
37	22199.0
38	25690.5
39	31829.5
40	37249.0
41	41071.5
42	46018.0
43	68623.0
44	88343.0
45	70322.5
46	50554.5
47	47439.5
48	48295.0
49	51167.5
50	57456.5
51	78228.0
52	93094.0
53	79150.5
54	62442.0
55	55723.0
56	51872.5
57	51575.0
58	52384.5
59	51537.5
60	48681.5
61	45448.0
62	42987.5
63	41366.5
64	42299.5
65	43649.5
66	38689.5
67	33198.5
68	29112.5
69	25708.0
70	22810.0
71	19953.5
72	19408.5
73	17334.5
74	13333.0
75	11331.0
76	10657.5
77	7998.0
78	5567.5
79	6128.5
80	6655.0
81	4298.5
82	2140.0
83	1975.5
84	1857.0
85	1520.0
86	1185.0
87	690.5
88	301.5
89	240.5
90	175.5
91	103.0
92	60.0
93	47.5
94	25.0
95	4.5
96	4.0
97	4.0
98	4.5
99	5.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	5615.0
25	4063.0
26	5547.0
27	9907.0
28	13824.0
29	21066.0
30	4576.0
31	3718.0
32	3461.0
33	3240.0
34	3502.0
35	3172.0
36	3234.0
37	2983.0
38	2845.0
39	3473.0
40	3704.0
41	4369.0
42	5477.0
43	5709.0
44	5582.0
45	5931.0
46	10563.0
47	33194.0
48	119842.0
49	222933.0
50	396017.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.699245158095348
#Duplication Level	Percentage of deduplicated	Percentage of total
1	56.10823637961767	10.49181667450835
2	9.706357531578314	3.630031181502162
3	5.77285765983602	3.23844241932187
4	4.585979486912954	3.4301741886312667
5	3.708935337416074	3.467714557493313
6	3.086157467302683	3.462528904654769
7	2.5241607114670677	3.3039929973508553
8	2.046328240782096	3.061183475865471
9	1.742534232935581	2.932566733623245
>10	10.130715751366083	31.695355848384054
>50	0.3362795242980466	4.364374711869468
>100	0.20393337877810153	7.5221850864106115
>500	0.02406293554903853	3.1616350237573894
>1k	0.021656641994134678	8.146727805114839
>5k	6.015733887259633E-4	0.6312920996700516
>10k+	0.0012031467774519266	7.459978291842269
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA	39417	4.3432461349109195	No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC	26900	2.9640338186341864	No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGC	5612	0.6183701780734221	No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCC	4035	0.444605072795128	No Hit
CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGG	4028	0.4438337628794983	No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA	3830	0.4220167109802578	No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGC	3772	0.4156258573936116	No Hit
CAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGCGC	3386	0.3730936249031731	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA	3111	0.34279216393200573	No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGA	2833	0.31216014156842564	No Hit
CGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGT	2738	0.30169236414202244	No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCC	2667	0.293869077854921	No Hit
ATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGC	2517	0.27734100823428426	No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGT	2470	0.2721622130864848	No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCG	2280	0.25122665823367824	No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG	1973	0.2173992090767751	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC	1818	0.20032020380211712	No Hit
ATCAGATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGG	1792	0.19745533840120677	No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA	1776	0.19569234430833887	Illumina Single End Adapter 1 (100% over 32bp)
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA	1751	0.19293766603823273	Illumina Single End Adapter 1 (100% over 32bp)
TACCTGGTTGATCCTGCCAGTAGCATATG	1693	0.18654681245158655	No Hit
AGGCCTGGTTTGCGCTGGAGAGTGGTCTTGGTTGTGAGGGTCATAAGAT	1587	0.17486697658633657	No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTGCAGGAGGGAAGCAGTGGT	1537	0.16935762004612434	No Hit
TGGAGAAGGGTTCCATGTGAACAGCAGTTGAACATGGGTCAGTCGGTCC	1533	0.16891687152290735	No Hit
AAGCAGTGGTATCAACGCAGAGTG	1523	0.1678150002148649	No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA	1486	0.16373807637510784	No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGC	1470	0.16197508228223992	No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGAC	1434	0.1580083455732871	No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGT	1397	0.15393142173353005	No Hit
CGCGACCTCAGATCAGACGTGGCGACCCG	1363	0.15018505928618572	No Hit
AAGCAGTGGTATCAACGCAGAGTGCAG	1356	0.14941374937055601	No Hit
CTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAATCCT	1317	0.14511645126919046	No Hit
AACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTT	1258	0.13861541055174004	No Hit
ATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAAT	1198	0.13200418270348532	No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCC	1164	0.12825782025614102	No Hit
TCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA	1154	0.12715594894809856	Illumina Single End Adapter 1 (100% over 31bp)
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAAT	1092	0.12032434683823538	No Hit
CGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGGGGAATCA	1070	0.11790022996054199	No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCT	1013	0.11161956350470004	No Hit
ACACCCGGGTGAGGGGTCTGCTGGCTGCACCTGTCGGTCTCATGGCTCC	991	0.10919544662700664	No Hit
CTTCCGCCACGGCCGTCTCTGGAGAGCAGCAGCCATGGCCCTACGCTAC	979	0.1078732010573557	No Hit
AGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTCGCT	964	0.10622039409529202	No Hit
TCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGC	939	0.1034657158251859	No Hit
CAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGG	934	0.10291478017116469	No Hit
CCCTGTAATTGGAATGAGTCCACTTTAAATCCTTTAACGAGGATCCAT	929	0.10236384451714345	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.24946366414081034	1.1018713080424484E-4	0.0	0.0	0.0
2	0.44581713123397465	1.1018713080424484E-4	0.0	0.0	0.0
3	0.5222870000121206	1.1018713080424484E-4	0.0	0.0	0.0
4	0.6091144590858655	1.1018713080424484E-4	0.0	0.0	0.0
5	0.7197423384133274	1.1018713080424484E-4	0.0	0.0	0.0
6	0.828497036517117	1.1018713080424484E-4	0.0	0.0	0.0
7	0.9160958055064917	1.1018713080424484E-4	0.0	0.0	0.0
8	1.002151954664607	1.1018713080424484E-4	0.0	0.0	0.0
9	1.0677132974931325	1.1018713080424484E-4	0.0	0.0	0.0
10	1.1297486521359223	1.1018713080424484E-4	0.0	0.0	0.0
11	1.198395234626967	1.1018713080424484E-4	0.0	0.0	0.0
12	1.2455553266111838	1.1018713080424484E-4	0.0	0.0	0.0
13	1.2944784126882685	1.1018713080424484E-4	0.0	0.0	0.0
14	1.3497923523519995	1.1018713080424484E-4	0.0	0.0	0.0
15	1.4087424673322704	1.1018713080424484E-4	0.0	0.0	0.0
16	1.4482996472909944	1.1018713080424484E-4	0.0	0.0	0.0
17	1.5211333407526002	1.1018713080424484E-4	0.0	0.0	0.0
18	1.550773678938942	1.1018713080424484E-4	0.0	0.0	0.0
19	1.6229462496157223	1.1018713080424484E-4	0.0	0.0	0.0
20	1.6455346114305927	1.1018713080424484E-4	0.0	0.0	0.0
21	1.6865242240897718	1.1018713080424484E-4	0.0	0.0	0.0
22	1.7076801532041868	1.1018713080424484E-4	0.0	0.0	0.0
23	1.7377612399137454	1.1018713080424484E-4	0.0	0.0	0.0
24	1.77213962472467	1.1018713080424484E-4	0.0	0.0	0.0
25	1.7995762202949268	1.1018713080424484E-4	0.0	0.0	0.0
26	1.8492706162876413	1.1018713080424484E-4	0.0	0.0	0.0
27	1.8697654226172309	1.1018713080424484E-4	0.0	0.0	0.0
28	1.888276860592344	1.1018713080424484E-4	0.0	0.0	0.0
29	1.9140606492005372	1.1018713080424484E-4	0.0	0.0	0.0
30	1.9320211515216292	1.1018713080424484E-4	0.0	0.0	0.0
31	1.9474473498342235	1.1018713080424484E-4	0.0	0.0	0.0
32	1.9596781213534946	1.1018713080424484E-4	0.0	0.0	0.0
33	1.9748839454044804	1.1018713080424484E-4	0.0	0.0	0.0
34	1.9917425764175298	1.1018713080424484E-4	0.0	0.0	0.0
35	2.0059567162912777	1.1018713080424484E-4	0.0	0.0	0.0
36	2.021493101734676	1.1018713080424484E-4	0.0	0.0	0.0
37	2.034935931692794	1.1018713080424484E-4	0.0	0.0	0.0
38	2.0491500715665416	1.1018713080424484E-4	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTAGTC	3035	0.0	89.9683	44
TAAGCCA	4410	0.0	88.83738	44
TTAAGCC	4765	0.0	57.400085	43
TATTAGT	3345	0.0	57.082787	43
ATATTAG	3360	0.0	47.77053	42
ATTAAGC	4780	0.0	47.706837	42
CATATTA	3355	0.0	45.90216	41
AACGACA	40	1.9948602E-6	45.534637	43
GATTAAG	4910	0.0	45.523376	41
AGATTAA	4865	0.0	44.78002	40
GCATATT	3390	0.0	44.69011	40
AAGCATA	3365	0.0	44.068886	38
TAAGCAT	3360	0.0	43.9501	37
CAATGAA	690	0.0	43.55487	43
AAGATTA	5050	0.0	42.932278	39
CAAAGAT	4980	0.0	42.91524	37
GTCTCAA	4890	0.0	42.84931	33
TTAAGCA	3440	0.0	42.82031	36
AAAGATT	5075	0.0	42.514496	38
CTCAAAG	4970	0.0	42.482727	35
>>END_MODULE