FastQCFastQC Report
Tue 24 May 2016
ERR841164.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841164.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3792722
Sequences flagged as poor quality0
Sequence length24-50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA2017815.320215929351004No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGC467891.233652242373683No Hit
CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGG359560.9480262460575808No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGC281760.7428965265579708No Hit
CGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGT245800.6480833554370713No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA242850.6403053005203123No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCG218560.5762615873243544No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC184050.48527152794220085No Hit
ATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGC173430.45727053024186853No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCC172530.4548975643350607No Hit
CAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGCGC172520.45487119804720727No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAAG159300.42001496550498557No Hit
ACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCAT124950.32944676672848683No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGAC117150.30888106220281897No Hit
AAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCGAATG116090.3060862356903564No Hit
CAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGG116090.3060862356903564No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCC99260.26171177323305006No Hit
AACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTT97360.25670217854090016No Hit
ACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTCG95830.25266813649932685No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGA93890.24755307665576334No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA93390.24623476226309232Illumina Single End Adapter 1 (100% over 32bp)
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA93000.24520647703680892Illumina Single End Adapter 1 (100% over 32bp)
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA92720.24446822097691315No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCCG89940.2371383929536623No Hit
TACCTGGTTGATCCTGCCAGTAGCATATG88940.23450176416832028No Hit
AGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTCGCT85190.22461440622328765No Hit
CGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTC80690.2127495766892485No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAAT78200.20618437101374687No Hit
TCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGC77920.2054461149538511No Hit
CGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGGGGAATCA75340.19864361268766864No Hit
ATCAGATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGG68690.18111003126514413No Hit
ATCCGAATTCAGTCTTCCTGAGGCTCTGGCTCACCTCCAGGCAGGTCCC65930.17383293581760012No Hit
CGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTG64800.17085354529016364No Hit
AGGCCTGGTTTGCGCTGGAGAGTGGTCTTGGTTGTGAGGGTCATAAGAT64390.1697725274881734No Hit
ACGCACGGCCGGTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATG58780.1549810400024046No Hit
ATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAAT56510.1489958926596782No Hit
CTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAATCCT56470.1488904275082645No Hit
ATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGGGGAATCAG54660.14411812940679544No Hit
CTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAATCCTG50980.13441533547673676No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTAG50300.13262242790270418No Hit
AGGCCTGGTTTGCGCTGGAGAGTGGTCTTGGTTGTGAGGGTCATAAGATG50000.13183143926710156No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA49530.13059222373799081No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG47920.12634725139359015No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCCC47480.12518713472803966No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTAGATGGGGGAAGCAGTGGT47160.12434341351673019No Hit
TTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAATTC46780.12334149457830022No Hit
ATCCGAATTCAGTCTTCCTGAGGCTCTGGCTCACCTCCAGGCAGGTCCCA45960.12117945897431977No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA44350.1169344866299191Illumina Single End Adapter 1 (100% over 32bp)
CGCTGACCCCCTTCGCGGGGGGGATGCGTGCATTTATCAGATCAAAA44090.11624896314573016No Hit
TCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA43440.11453515443525784Illumina Single End Adapter 1 (100% over 31bp)
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAAT43060.11353323549682787No Hit
AGTTATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGG42300.11152939761996793No Hit
CCCTTCCACCTTCTGCCGTGTTAGGATTTGGTGTTCCTCCCTTCCAGA40830.10765355330551514No Hit
AACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGGG38860.10245939459839135No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC38780.10224846429556399No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGT38670.10195843512917635No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGCCA245700.077.4335444
ATTAGTC22800.074.12611444
CTATACG3750.062.04259544
TTAAGCC244700.053.07028643
CAATGAA49900.051.3212543
AATGAAG29900.051.23887644
TATTAGT22950.050.47382743
AATCCCG13200.045.3441743
ATTAAGC245300.044.31564342
TATACGC1200.043.89806744
CGACGGG75900.043.66671844
ATAAGAT16600.043.41168243
GCAATGA50300.042.7682942
GATTAAG251250.042.06318341
ATATTAG23550.041.71381842
AGATTAA248950.041.69551540
AAGATTA250000.041.35148639
AAAGATT251500.040.9294538
TAAGCAT22850.040.8235137
CATATTA23200.040.66343341
Produced by FastQC (version 0.11.5)