FastQCFastQC Report
Tue 24 May 2016
ERR841163.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841163.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9683030
Sequences flagged as poor quality0
Sequence length24-50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA9100599.398494066423423No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCC1476031.5243472342851359No Hit
CTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAATCCT789370.8152097019218157No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCCG717670.7411626319447528No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATC635700.6565093777464286No Hit
CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGG519610.5366192193972341No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGC513470.5302782290254187No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGC487420.5033754930016741No Hit
ATCCGAATTCAGTCTTCCTGAGGCTCTGGCTCACCTCCAGGCAGGTCCC461130.476224900676751No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAAT452130.466930289382559No Hit
CTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAATCCTG444670.459226089354262No Hit
TACCTGGTTGATCCTGCCAGTAGCATATG418830.4325402275940486No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGA344810.3560972133722605No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGAC334730.3456872487227655No Hit
CGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGT298650.308426184778938No Hit
TCGCTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAAT293410.3030146555365418No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA293050.30264287108477406No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA283710.29299713003057926No Hit
ATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGC273110.2820501433951976No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCC236990.24474777006784032No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCG231430.23900576575720617No Hit
CAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGCGC219290.2264683678559294No Hit
TCGCTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAATC212660.2196213375358746No Hit
ACTACTTCTCCCCCGGACTCCTTGGTAGTCTGTTAGTGGGAGATCCTTG197980.20446079378045923No Hit
AAGCAGTGGTATCAACGCAGAGTGCAG187110.19323496880625177No Hit
TCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA185200.19126244574270657Illumina Single End Adapter 1 (100% over 31bp)
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA172510.17815704381789585Illumina Single End Adapter 1 (100% over 32bp)
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA167360.17283846068844153No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA160360.16560931857073663Illumina Single End Adapter 1 (100% over 32bp)
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTAG156250.16136477941305563No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTG155020.16009451586951604No Hit
TCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA154530.1595884759212767Illumina Single End Adapter 1 (100% over 31bp)
TCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGC154410.15946454777068747No Hit
CTGTGCGTCTGCTCCTCCTTCTCGCGCTTCTCCTGCCGCCCTAATCCT152470.15746104266949498No Hit
AAGCAGTGGTATCAACGCAGAGTG142880.14755711796823928No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAAG141500.14613194423646317No Hit
ATCCGAATTCAGTCTTCCTGAGGCTCTGGCTCACCTCCAGGCAGGTCCCA120220.1241553521986403No Hit
CCCTTCCACCTTCTGCCGTGTTAGGATTTGGTGTTCCTCCCTTCCAGA114780.11853727603859535No Hit
CGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTG113870.11759748756329372No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGC111940.11560430980798367No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG109050.11261970684795979No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTG103360.10674344704085395No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCC102320.10566940306908064No Hit
ACACCCGGGTGAGGGGTCTGCTGGCTGCACCTGTCGGTCTCATGGCTCC101930.10526663657966567No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCT100840.1041409558784802No Hit
AGGCCTGGTTTGCGCTGGAGAGTGGTCTTGGTTGTGAGGGTCATAAGAT100730.1040273550737734No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGCCA991300.0123.59749644
CGACGGG109550.066.8908944
TTAAGCC998500.064.58166543
ATTAGTC8350.063.7145844
CTATACG2500.058.26850544
AATCCCG93450.054.54128643
AGGTCCC80250.053.4194843
AATGAAG40400.051.8910744
GGTCCCA36000.050.316444
AATCCTG79900.050.31559443
ATTAAGC1000500.050.2191942
CAATGAA62150.049.42813543
GATTAAG1017150.047.84541341
AGATTAA1009100.047.38152740
ATCCTTG38700.046.87857443
AAGATTA1014550.046.82205239
TAATCCT184050.046.58273742
TAATCCC257250.046.55141442
AAAGATT1020000.046.3101438
TTTGGAC50200.046.24013543
Produced by FastQC (version 0.11.5)