##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841163.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9683030
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.51816642104796	38.0	35.0	39.0	32.0	39.0
2	37.51560296725302	39.0	37.0	39.0	35.0	39.0
3	37.70428058159481	39.0	37.0	39.0	35.0	40.0
4	38.04021602742117	39.0	37.0	40.0	35.0	41.0
5	38.50489691759707	40.0	38.0	41.0	35.0	41.0
6	38.43787709012572	40.0	38.0	41.0	34.0	41.0
7	38.382148046634164	40.0	38.0	41.0	34.0	41.0
8	38.207222842436714	39.0	37.0	41.0	34.0	41.0
9	38.33105494870924	40.0	38.0	41.0	34.0	41.0
10	38.238288841406046	40.0	38.0	41.0	34.0	41.0
11	38.42297917077609	40.0	38.0	41.0	34.0	41.0
12	38.407115541313	40.0	38.0	41.0	34.0	41.0
13	38.43740626642693	40.0	38.0	41.0	34.0	41.0
14	38.45362267802537	40.0	38.0	41.0	34.0	41.0
15	38.49263329763514	40.0	38.0	41.0	35.0	41.0
16	38.269091183235	40.0	38.0	41.0	34.0	41.0
17	38.23765505218924	40.0	37.0	41.0	34.0	41.0
18	38.32836477838032	40.0	38.0	41.0	34.0	41.0
19	38.02820604707411	39.0	37.0	40.0	33.0	41.0
20	38.073996982349534	39.0	37.0	40.0	34.0	41.0
21	37.545600499017354	39.0	36.0	40.0	33.0	41.0
22	37.54095412283139	39.0	36.0	40.0	33.0	41.0
23	37.50486407663717	39.0	36.0	40.0	33.0	41.0
24	37.26082145774618	38.0	35.0	40.0	32.0	41.0
25	37.3050262438905	39.0	35.0	40.0	32.0	41.0
26	37.097687456993995	38.0	35.0	40.0	32.0	41.0
27	37.059211829365644	38.0	35.0	40.0	32.0	41.0
28	37.36932426512355	39.0	35.0	40.0	33.0	41.0
29	37.61207997515224	39.0	35.0	40.0	33.0	41.0
30	37.71310667659028	39.0	35.0	41.0	33.0	41.0
31	37.784186183387476	39.0	35.0	41.0	33.0	41.0
32	37.803387814201585	39.0	35.0	41.0	33.0	41.0
33	37.71279285624117	39.0	35.0	41.0	33.0	41.0
34	37.602461322581256	39.0	35.0	41.0	33.0	41.0
35	37.54552314347034	39.0	35.0	41.0	33.0	41.0
36	37.390282284355195	39.0	35.0	41.0	33.0	41.0
37	37.41770231840007	39.0	35.0	41.0	33.0	41.0
38	37.27187706547157	38.0	35.0	41.0	33.0	41.0
39	37.11427825706001	38.0	35.0	41.0	33.0	41.0
40	37.06635915551071	38.0	35.0	41.0	33.0	41.0
41	36.85322778994781	37.0	35.0	40.0	33.0	41.0
42	36.70206786621059	37.0	35.0	40.0	33.0	41.0
43	36.67819444169964	37.0	35.0	40.0	33.0	41.0
44	36.67185162587655	37.0	35.0	40.0	33.0	41.0
45	36.55173017798091	37.0	35.0	40.0	33.0	41.0
46	36.40213678103504	36.0	35.0	40.0	33.0	41.0
47	36.321317423715975	36.0	35.0	40.0	33.0	41.0
48	36.27346268095703	36.0	35.0	40.0	32.0	41.0
49	36.63072158202133	37.0	35.0	40.0	32.0	41.0
50	37.277546864507386	39.0	35.0	40.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	3.0
14	206.0
15	1209.0
16	1594.0
17	31.0
18	0.0
19	1.0
20	12.0
21	29.0
22	137.0
23	397.0
24	1066.0
25	2513.0
26	5851.0
27	12395.0
28	25342.0
29	49370.0
30	90710.0
31	154749.0
32	249047.0
33	394897.0
34	652995.0
35	702069.0
36	942967.0
37	1323357.0
38	1717997.0
39	1961001.0
40	1393085.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	40.57943639542581	27.16032068474434	32.26024291982984
2	24.400316842971673	27.607959492018512	27.104046977030947	20.887676687978864
3	32.717073064939385	12.390419114677947	19.911618573938117	34.980889246444555
4	19.335600529999393	15.158860398036564	24.30827953646741	41.19725953549664
5	26.709852184698384	17.16213829761965	35.32795003216968	20.800059485512282
6	36.86078634477018	18.41898661885794	19.091606656180968	25.628620380190913
7	39.959568440870264	18.182583344263108	19.6380471815124	22.219801033354226
8	28.57171773711328	14.703135278936449	34.58358592300137	22.1415610609489
9	23.842020524567207	17.45666387484083	31.23852760964285	27.462787990949117
10	36.143046133286795	18.578399529899215	21.821072536179276	23.45748180063472
11	30.279148159202236	27.014322996004346	22.043482257103406	20.66304658769001
12	24.767433334400494	13.788855347964429	31.162394415797536	30.281316901837545
13	22.675949573635528	14.833156563596312	26.745667420218673	35.745226442549495
14	23.909210236878334	18.329696386358403	18.445548552467564	39.3155448242957
15	22.69263856458154	18.02078481632299	30.351511871800458	28.93506474729501
16	34.465895489325135	17.153504636461932	26.13144852386082	22.24915135035211
17	25.62085421608732	16.10428760419001	17.590051874258368	40.6848063054643
18	25.478243896796766	16.39725375218294	16.140650189042066	41.98385216197823
19	23.205009175846815	28.097186521161248	27.29646608551249	21.401338217479445
20	40.005886587152986	14.065772800456056	21.369922431305078	24.558418181085877
21	25.184203704832065	17.42765436025707	29.009421637648547	28.378720297262323
22	25.572429291244582	26.159859052383393	26.471290494814127	21.796421161557898
23	36.251679484624134	15.866851595006933	21.29666023961508	26.58480868075386
24	29.411764705882355	18.42133092637325	18.116240474314342	34.05066389343005
25	24.71622519063958	25.96432878139634	23.096350488884525	26.223095539079555
26	29.519835778024845	16.67321547707543	29.934284414591822	23.872664330307902
27	25.873898818741225	28.573799786952392	22.50889676898896	23.04340462531742
28	25.264218433008374	15.023745188937596	34.403977209250364	25.308059168803666
29	41.15066581138384	15.874801494590299	23.642310644053165	19.332222049972692
30	24.075604550542607	17.890208267013897	19.975005853279367	38.05918132916413
31	23.863100688973894	16.339988426969125	39.6513421651302	20.145568718926775
32	25.774681238202923	15.592419961161704	31.67453327200176	26.95836552863361
33	34.30790246447911	16.791635159888322	20.016929559871354	28.88353281576121
34	21.55604968074526	17.61649640587721	39.023387870726644	21.80406604265088
35	27.459616008673272	18.098214088163495	19.418564559071903	35.02360534409134
36	23.198724001696938	17.50404925114078	31.86758908178548	27.4296376653768
37	22.25338171836112	17.64207782914041	21.674864912965653	38.429675539532816
38	30.110758993904046	29.415953366111818	18.002816937656537	22.4704707023276
39	22.772168089157933	31.135225120583105	19.704590783647983	26.388016006610975
40	24.07293614952541	30.72546211968545	17.22449695635025	27.977104774438892
41	36.134352055345005	18.569645650797735	18.22645716546748	27.069545128389784
42	24.443912687915308	29.36890912007336	24.19839496117925	21.98878323083208
43	21.40569123991927	22.744582520134944	31.602102450223583	24.2476237897222
44	23.027869150692972	21.47987446604227	30.672205634357695	24.820050748907065
45	23.094082613330496	28.886006227282984	27.389745838539554	20.630165320846963
46	23.563131303159775	32.36774963869124	18.69295319570694	25.37616586244205
47	37.920896319198874	17.64765551631764	17.67570744394217	26.75574072054131
48	21.345477183622116	18.46146718106405	21.69222556916957	38.50083006614427
49	25.432752356008688	17.66115271186649	16.566921963037736	40.33917296908708
50	19.07743267555331	47.6109021155229	15.765562395246771	17.546102813677024
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2381.0
1	2712.5
2	5332.5
3	7399.5
4	6816.5
5	4682.5
6	2503.5
7	2281.0
8	2617.0
9	2902.5
10	2954.0
11	2741.0
12	2480.0
13	2519.5
14	2771.5
15	3186.5
16	3703.0
17	4768.5
18	5809.5
19	7441.5
20	9083.5
21	10830.5
22	12844.5
23	15856.0
24	18640.0
25	21671.0
26	26227.5
27	31549.0
28	36661.0
29	41318.5
30	47656.0
31	56363.5
32	65656.0
33	74167.5
34	82964.0
35	93455.0
36	109822.0
37	126598.0
38	157295.5
39	216315.0
40	272712.0
41	321238.5
42	365986.0
43	834352.5
44	1273844.0
45	851662.5
46	441508.5
47	416955.0
48	427570.0
49	487891.0
50	552066.5
51	598419.0
52	633021.5
53	686835.5
54	684834.0
55	634108.5
56	637083.0
57	735471.0
58	850826.5
59	798663.0
60	635099.5
61	562434.0
62	552670.5
63	540161.5
64	617538.0
65	700756.0
66	578380.5
67	432479.5
68	367477.0
69	322369.0
70	296349.0
71	289966.5
72	300558.0
73	262138.5
74	198081.5
75	172072.5
76	165911.0
77	127842.5
78	95221.0
79	89989.0
80	83437.0
81	59607.0
82	39196.0
83	35471.5
84	31664.5
85	25494.0
86	19578.5
87	11535.5
88	5164.0
89	3692.5
90	2497.5
91	1474.5
92	962.0
93	810.0
94	535.0
95	234.5
96	159.5
97	152.0
98	92.0
99	31.5
100	31.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	70126.0
25	59006.0
26	77143.0
27	149813.0
28	199357.0
29	196694.0
30	55083.0
31	49468.0
32	57063.0
33	43643.0
34	47938.0
35	55114.0
36	37006.0
37	37286.0
38	38806.0
39	38897.0
40	44605.0
41	59580.0
42	67894.0
43	64242.0
44	58951.0
45	71443.0
46	118681.0
47	323151.0
48	1723713.0
49	2866226.0
50	3072101.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	10.187842545444452
#Duplication Level	Percentage of deduplicated	Percentage of total
1	44.234816669252375	4.506573472529447
2	11.014304765534318	2.244240053976042
3	7.278169013958709	2.224465198000321
4	5.795774260039415	2.3618574236088556
5	4.7808303392060685	2.435317336615761
6	3.902815501852646	2.385676189007672
7	3.214813263432427	2.2926407938599205
8	2.6510745306117314	2.1606983915288303
9	2.2269220315794436	2.041877791684138
>10	13.645806722969247	24.157662475505056
>50	0.7010687983655018	4.927576478956409
>100	0.45308943056182593	9.128267061649247
>500	0.04866693835737936	3.438962361429512
>1k	0.04251726267047286	8.546727463881583
>5k	0.004453179631311802	3.1183788112208113
>10k+	0.004877291977151021	24.029078696546367
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA	910059	9.398494066423423	No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCC	147603	1.5243472342851359	No Hit
CTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAATCCT	78937	0.8152097019218157	No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCCG	71767	0.7411626319447528	No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATC	63570	0.6565093777464286	No Hit
CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGG	51961	0.5366192193972341	No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGC	51347	0.5302782290254187	No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGC	48742	0.5033754930016741	No Hit
ATCCGAATTCAGTCTTCCTGAGGCTCTGGCTCACCTCCAGGCAGGTCCC	46113	0.476224900676751	No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAAT	45213	0.466930289382559	No Hit
CTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAATCCTG	44467	0.459226089354262	No Hit
TACCTGGTTGATCCTGCCAGTAGCATATG	41883	0.4325402275940486	No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGA	34481	0.3560972133722605	No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGAC	33473	0.3456872487227655	No Hit
CGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGT	29865	0.308426184778938	No Hit
TCGCTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAAT	29341	0.3030146555365418	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA	29305	0.30264287108477406	No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA	28371	0.29299713003057926	No Hit
ATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGC	27311	0.2820501433951976	No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCC	23699	0.24474777006784032	No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCG	23143	0.23900576575720617	No Hit
CAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGCGC	21929	0.2264683678559294	No Hit
TCGCTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAATC	21266	0.2196213375358746	No Hit
ACTACTTCTCCCCCGGACTCCTTGGTAGTCTGTTAGTGGGAGATCCTTG	19798	0.20446079378045923	No Hit
AAGCAGTGGTATCAACGCAGAGTGCAG	18711	0.19323496880625177	No Hit
TCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA	18520	0.19126244574270657	Illumina Single End Adapter 1 (100% over 31bp)
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA	17251	0.17815704381789585	Illumina Single End Adapter 1 (100% over 32bp)
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA	16736	0.17283846068844153	No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA	16036	0.16560931857073663	Illumina Single End Adapter 1 (100% over 32bp)
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTAG	15625	0.16136477941305563	No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTG	15502	0.16009451586951604	No Hit
TCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA	15453	0.1595884759212767	Illumina Single End Adapter 1 (100% over 31bp)
TCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGC	15441	0.15946454777068747	No Hit
CTGTGCGTCTGCTCCTCCTTCTCGCGCTTCTCCTGCCGCCCTAATCCT	15247	0.15746104266949498	No Hit
AAGCAGTGGTATCAACGCAGAGTG	14288	0.14755711796823928	No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAAG	14150	0.14613194423646317	No Hit
ATCCGAATTCAGTCTTCCTGAGGCTCTGGCTCACCTCCAGGCAGGTCCCA	12022	0.1241553521986403	No Hit
CCCTTCCACCTTCTGCCGTGTTAGGATTTGGTGTTCCTCCCTTCCAGA	11478	0.11853727603859535	No Hit
CGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTG	11387	0.11759748756329372	No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGC	11194	0.11560430980798367	No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG	10905	0.11261970684795979	No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTG	10336	0.10674344704085395	No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCC	10232	0.10566940306908064	No Hit
ACACCCGGGTGAGGGGTCTGCTGGCTGCACCTGTCGGTCTCATGGCTCC	10193	0.10526663657966567	No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCT	10084	0.1041409558784802	No Hit
AGGCCTGGTTTGCGCTGGAGAGTGGTCTTGGTTGTGAGGGTCATAAGAT	10073	0.1040273550737734	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.246162616453734	0.0	0.0	0.0	0.0
2	0.36516462305703895	0.0	0.0	0.0	0.0
3	0.4148804661350837	0.0	0.0	0.0	7.229142117704891E-5
4	0.47917852159912755	0.0	0.0	0.0	7.229142117704891E-5
5	0.5716908860139853	0.0	0.0	0.0	7.229142117704891E-5
6	0.6606403160994028	0.0	0.0	0.0	7.229142117704891E-5
7	0.7355548831305904	0.0	0.0	0.0	7.229142117704891E-5
8	0.8075984480064607	0.0	0.0	0.0	7.229142117704891E-5
9	0.8604847862704132	0.0	0.0	0.0	7.229142117704891E-5
10	0.9091678947602145	0.0	0.0	0.0	7.229142117704891E-5
11	0.9663090995277305	0.0	0.0	0.0	7.229142117704891E-5
12	1.0014737122574235	0.0	0.0	0.0	7.229142117704891E-5
13	1.0405627164224422	0.0	0.0	0.0	7.229142117704891E-5
14	1.0819753734110087	0.0	0.0	0.0	7.229142117704891E-5
15	1.1298632762678624	0.0	0.0	0.0	7.229142117704891E-5
16	1.159420140183393	0.0	0.0	0.0	7.229142117704891E-5
17	1.217439169350916	0.0	0.0	0.0	7.229142117704891E-5
18	1.2419253064381708	0.0	0.0	0.0	7.229142117704891E-5
19	1.2986121079868596	0.0	0.0	0.0	7.229142117704891E-5
20	1.317191003229361	0.0	0.0	0.0	7.229142117704891E-5
21	1.3501352365943304	0.0	0.0	0.0	7.229142117704891E-5
22	1.3672373213756437	0.0	0.0	0.0	7.229142117704891E-5
23	1.390029773738179	0.0	0.0	0.0	7.229142117704891E-5
24	1.4132972840113063	0.0	0.0	0.0	7.229142117704891E-5
25	1.43074017120674	0.0	0.0	0.0	7.229142117704891E-5
26	1.4694367362282261	0.0	0.0	0.0	7.229142117704891E-5
27	1.4892445856307375	0.0	0.0	0.0	7.229142117704891E-5
28	1.5043018559273285	0.0	0.0	0.0	7.229142117704891E-5
29	1.5267535058757435	0.0	0.0	0.0	7.229142117704891E-5
30	1.5398692351464367	0.0	0.0	0.0	7.229142117704891E-5
31	1.551353243767705	0.0	0.0	0.0	7.229142117704891E-5
32	1.5617012443419054	0.0	0.0	0.0	7.229142117704891E-5
33	1.57257593955611	0.0	0.0	0.0	7.229142117704891E-5
34	1.5853405390668003	0.0	0.0	0.0	7.229142117704891E-5
35	1.5974441884410149	0.0	0.0	0.0	7.229142117704891E-5
36	1.6101158418387633	0.0	0.0	0.0	7.229142117704891E-5
37	1.6207116987141421	0.0	0.0	0.0	7.229142117704891E-5
38	1.6296655075942137	0.0	0.0	0.0	7.229142117704891E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAAGCCA	99130	0.0	123.597496	44
CGACGGG	10955	0.0	66.89089	44
TTAAGCC	99850	0.0	64.581665	43
ATTAGTC	835	0.0	63.71458	44
CTATACG	250	0.0	58.268505	44
AATCCCG	9345	0.0	54.541286	43
AGGTCCC	8025	0.0	53.41948	43
AATGAAG	4040	0.0	51.89107	44
GGTCCCA	3600	0.0	50.3164	44
AATCCTG	7990	0.0	50.315594	43
ATTAAGC	100050	0.0	50.21919	42
CAATGAA	6215	0.0	49.428135	43
GATTAAG	101715	0.0	47.845413	41
AGATTAA	100910	0.0	47.381527	40
ATCCTTG	3870	0.0	46.878574	43
AAGATTA	101455	0.0	46.822052	39
TAATCCT	18405	0.0	46.582737	42
TAATCCC	25725	0.0	46.551414	42
AAAGATT	102000	0.0	46.31014	38
TTTGGAC	5020	0.0	46.240135	43
>>END_MODULE