FastQCFastQC Report
Tue 24 May 2016
ERR841162.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841162.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6361550
Sequences flagged as poor quality0
Sequence length24-50
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA3907646.142591035203685No Hit
CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGG494220.7768861362403816No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGC429620.6753385574270422No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTAGTTTAGGGAAGCAGTGGT387930.6098042143817152No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGC329280.5176097020380254No Hit
CGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGT295780.46494957989798086No Hit
ATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGC232610.3656498809252462No Hit
CAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGCGC205500.32303448059042217No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCG189550.29796197467598307No Hit
TACCTGGTTGATCCTGCCAGTAGCATATG183660.2887032248430021No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCC183290.2881216055835449No Hit
AAGCAGTGGTATCAACGCAGAGTGCAG178980.2813465271828407No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCC156520.24604066618984366No Hit
AAGCAGTGGTATCAACGCAGAGTG154400.24270814502754834No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA154250.24247235343587648Illumina Single End Adapter 1 (100% over 32bp)
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA153630.24149774819029954Illumina Single End Adapter 1 (100% over 32bp)
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA144590.2272873749322099No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA142260.22362474554157402No Hit
CGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTG137720.2164881200336396No Hit
CGAGGCCCTGTAATTGGAATGAGTCCACTTTAAATCCTTTAACGAGGATC136540.21463322617915445No Hit
TCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGC127580.20054860843662314No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGAC115810.182046828210106No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGA110390.17352689203103017No Hit
TCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA108280.17021009030817963Illumina Single End Adapter 1 (100% over 31bp)
ATCCGAATTCAGTCTTCCTGAGGCTCTGGCTCACCTCCAGGCAGGTCCC104900.16489691977584078No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTG102560.16121857094576006No Hit
CGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGGGGAATCA95600.15027784109218667No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCT94740.14892596929993476No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCCG90550.142339524172568No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA89960.14141207724532545No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTAG86250.13558016521130856No Hit
AACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTT81390.12794051764114092No Hit
TCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA80900.12717026510834623Illumina Single End Adapter 1 (100% over 31bp)
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG79640.12518961573830278No Hit
CAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGG78400.12324040524714888No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTAG77750.12221864168323758No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAAG75200.11821018462481628No Hit
AGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA73440.11544356328253334Illumina Single End Adapter 2 (100% over 34bp)
CCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGG73030.11479906626529698No Hit
AGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTCGCT73030.11479906626529698No Hit
ACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTCG72480.11393449709583357No Hit
CTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAATCCT71960.11311708624470451No Hit
ATCAGATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGG70660.11107355911688188No Hit
AGGCCTGGTTTGCGCTGGAGAGTGGTCTTGGTTGTGAGGGTCATAAGAT69220.10880995983683223No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGGTTGTGAGGGTTCCTGCTT67840.10664067719345129No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA65830.10348106986504861Illumina Single End Adapter 1 (100% over 32bp)
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATC64830.10190912592056968No Hit
ACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTAC64200.10091880123554793No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGCCA434650.089.06402644
TTAAGCC438650.057.99541543
CGACGGG100000.049.33062444
ATTAAGC438000.048.7437742
GATTAAG447100.046.627241
AGATTAA443850.046.00798440
AAGATTA451500.045.0078439
AAAGATT450650.044.749338
CAAAGAT451850.044.29685637
CAATGAA33050.044.2386143
ATTAGTC5200.044.06125644
GTCTCAA445700.043.75683233
CTCAAAG450800.043.67466435
TCAAAGA454450.043.5517436
TCTCAAA451800.043.2219434
TTGTCTC449000.043.0040731
TGTCTCA451000.042.9641432
GCTTGTC448000.042.95631829
ATGCTTG444150.042.90157327
TATGCTT442500.042.8721126
Produced by FastQC (version 0.11.5)