##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841162.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6361550
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.40591884053415	39.0	37.0	39.0	34.0	39.0
2	37.978044501733066	39.0	38.0	39.0	35.0	39.0
3	38.270844369689776	39.0	38.0	39.0	35.0	40.0
4	38.57603775809355	39.0	38.0	40.0	35.0	41.0
5	39.37910729303393	40.0	39.0	41.0	36.0	41.0
6	39.30158216158012	40.0	39.0	41.0	36.0	41.0
7	39.167231413727784	40.0	39.0	41.0	35.0	41.0
8	39.19686931644018	40.0	39.0	41.0	35.0	41.0
9	39.210692991487925	40.0	39.0	41.0	35.0	41.0
10	39.08349490297176	40.0	39.0	41.0	35.0	41.0
11	39.21522097601999	40.0	39.0	41.0	35.0	41.0
12	39.2284796944141	40.0	39.0	41.0	35.0	41.0
13	39.243249365327635	40.0	39.0	41.0	35.0	41.0
14	39.220023264770376	40.0	39.0	41.0	35.0	41.0
15	39.216112268236515	40.0	39.0	41.0	35.0	41.0
16	39.160978535105436	40.0	38.0	41.0	35.0	41.0
17	39.06766244075736	40.0	38.0	41.0	35.0	41.0
18	39.04115710793753	40.0	38.0	41.0	35.0	41.0
19	38.8881456563259	40.0	38.0	41.0	35.0	41.0
20	38.88466222854493	40.0	38.0	41.0	35.0	41.0
21	38.60264573885295	40.0	38.0	41.0	35.0	41.0
22	38.53339563471167	40.0	38.0	41.0	34.0	41.0
23	38.37848024459448	40.0	38.0	41.0	34.0	41.0
24	38.278262844746955	40.0	37.0	41.0	34.0	41.0
25	38.27827919175165	40.0	37.0	41.0	34.0	41.0
26	38.180855642510664	40.0	37.0	41.0	34.0	41.0
27	38.14187210863036	40.0	37.0	41.0	34.0	41.0
28	38.202900709496596	40.0	37.0	41.0	34.0	41.0
29	38.327791298105105	40.0	37.0	41.0	34.0	41.0
30	38.43232283846021	40.0	37.0	41.0	34.0	41.0
31	38.43889335334072	40.0	37.0	41.0	34.0	41.0
32	38.4470923705408	40.0	37.0	41.0	35.0	41.0
33	38.40098205679671	40.0	36.0	41.0	35.0	41.0
34	38.28961227265262	40.0	36.0	41.0	34.0	41.0
35	38.2171390899218	40.0	36.0	41.0	34.0	41.0
36	38.15524594884428	40.0	35.0	41.0	34.0	41.0
37	38.10971003543643	40.0	35.0	41.0	34.0	41.0
38	38.00343932069389	40.0	35.0	41.0	34.0	41.0
39	37.874048144782	39.0	35.0	41.0	34.0	41.0
40	37.71487717643984	39.0	35.0	41.0	34.0	41.0
41	37.581335138784276	39.0	35.0	41.0	33.0	41.0
42	37.48206351558061	39.0	35.0	41.0	33.0	41.0
43	37.38450376109184	39.0	35.0	41.0	33.0	41.0
44	37.30792581130488	38.0	35.0	41.0	33.0	41.0
45	37.21789108696552	38.0	35.0	41.0	33.0	41.0
46	37.147042365612414	37.0	35.0	41.0	33.0	41.0
47	37.010632136332845	37.0	35.0	41.0	33.0	41.0
48	36.982863468646165	37.0	35.0	41.0	33.0	41.0
49	37.274398575541916	38.0	35.0	41.0	33.0	41.0
50	37.491461516926904	39.0	35.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	3.0
14	113.0
15	1276.0
16	1974.0
17	74.0
18	0.0
19	0.0
20	6.0
21	15.0
22	51.0
23	124.0
24	270.0
25	734.0
26	1954.0
27	4554.0
28	9982.0
29	19767.0
30	36356.0
31	61436.0
32	97511.0
33	156498.0
34	320228.0
35	312839.0
36	437077.0
37	651154.0
38	1006463.0
39	1408778.0
40	1832313.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	47.30534225149531	20.592402794916335	32.10225495358836
2	26.186118163026308	28.9285787268826	26.46230871407126	18.42299439601984
3	31.029246017087033	14.974526648379719	21.418396459982237	32.57783087455101
4	21.63364274429974	17.307715886851476	25.892479034197642	35.16616233465115
5	24.44132326241246	21.8536834576479	31.477721624446875	22.227271655492764
6	34.464996738216314	22.480354630553876	20.440049987817435	22.614598643412375
7	35.725161320747304	19.208133237968735	22.864427694508414	22.20227774677555
8	30.744236860513553	17.97925033993288	28.019617860427097	23.25689493912647
9	26.274304218311574	21.941822354614835	29.605237717223005	22.178635709850585
10	32.82198520800748	18.575410080876516	23.75682027178911	24.84578443932689
11	27.339814982197737	28.019617860427097	22.33399092988344	22.30657622749173
12	25.269250418530078	17.599688755098995	31.184915625908783	25.94614520046215
13	23.91010052581525	17.136091046993265	23.56370695820987	35.39010146898162
14	22.634169345521137	23.207190071602046	21.59176615761882	32.566874425258
15	22.878025009628157	22.537101806949565	29.612264306654822	24.972608876767456
16	31.54885208793454	21.7438674536866	20.863248736550055	25.8440317218288
17	28.180757834175633	19.47767446612854	20.58185505104888	31.75971264864695
18	26.57478130329872	20.358749047008985	18.61335680769624	34.45311284199605
19	23.447933286698998	29.73545755358364	23.326642091943	23.489967067774366
20	37.80185646579843	16.80470954405766	20.1087628015185	25.284671188625413
21	27.276780030024128	20.788659996384528	27.091070572423387	24.843489401167954
22	26.972577437888567	25.45112433290629	23.682891748080266	23.89340648112488
23	33.287799357074924	18.95234651932312	22.638256399776786	25.121597723825168
24	29.585934245584806	19.725868695522315	21.328402669160816	29.359794389732063
25	25.548987522072387	25.312098490653145	23.209935764832935	25.92897822244154
26	26.48373776995111	20.621880900462184	28.452799481236134	24.441581848350573
27	26.884079809996	26.929918036390788	22.277170181010632	23.908831972602588
28	25.489532573521164	17.935541551961666	30.477898681600184	26.097027192916983
29	36.79993611233691	19.056883072403824	22.218367373821867	21.924813441437408
30	25.59525611150688	20.966873842887523	21.82590805799221	31.61196198761339
31	24.570857869892667	19.508814820500735	33.29560437544961	22.624722934156996
32	25.438434579937937	19.816155346768376	28.446523778829775	26.298886294463912
33	32.8186196322175	20.113795788441024	21.822241888512714	25.245342690828764
34	22.551319744122114	21.47926192703961	32.54091180065005	23.428506528188223
35	24.312512454249298	22.114158916826995	23.00030418232519	30.573024446598517
36	24.091710286387848	21.44592315989538	30.919169084063185	23.543197469653585
37	24.219691907198776	21.86944179335205	22.3097189338014	31.601147365647776
38	26.412823882934234	29.355418006850275	20.679011967414347	23.552746142801144
39	24.68379292762675	30.248137505499116	20.10305917149467	24.965010395379455
40	25.372805681588428	31.102617687334295	19.809065714555626	23.71551091652165
41	31.177694513012987	23.242038028819742	20.3769677870477	25.203299671119566
42	26.46969832091923	29.57150565028346	21.594357347834816	22.3644386809625
43	24.19946849241025	22.106679879284716	29.161623350299536	24.532228278005494
44	25.294898573492752	21.155001095412818	28.460932274422163	25.08916805667226
45	25.34328591109273	28.972133099732496	24.33145922748353	21.353121761691245
46	26.93957040826751	29.895181922793494	20.07733840892813	23.087909260010868
47	35.58069126792328	19.655286658932955	20.712837819467993	24.05118425367578
48	24.364439908171594	21.95771723478557	21.027997395446388	32.64984546159645
49	26.80496909159722	20.84645147046819	19.477882681647163	32.87069675628743
50	23.02974642703116	35.65135995659149	20.851682926240493	20.46721069013685
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3970.0
1	3824.0
2	5103.0
3	6174.0
4	5534.0
5	4021.0
6	2433.0
7	2190.5
8	2392.5
9	2547.0
10	2663.5
11	2735.5
12	2726.0
13	2850.5
14	3012.5
15	3427.0
16	3994.0
17	5516.5
18	6943.0
19	8211.0
20	9595.5
21	11891.0
22	14333.5
23	17718.0
24	21006.5
25	24704.0
26	29443.0
27	34085.0
28	38818.5
29	45313.0
30	52221.0
31	61062.0
32	70893.0
33	80668.5
34	90779.5
35	101847.0
36	117095.0
37	134225.0
38	163283.0
39	210667.0
40	253571.5
41	292150.5
42	325986.0
43	529027.0
44	710958.5
45	530448.5
46	362959.0
47	344257.0
48	349521.0
49	408389.0
50	482459.0
51	500542.5
52	472700.5
53	461764.0
54	435276.5
55	384327.0
56	351797.5
57	353247.0
58	368218.0
59	372518.0
60	356442.5
61	337605.0
62	317883.0
63	298445.5
64	311545.5
65	328099.5
66	286074.0
67	238326.0
68	205682.0
69	181945.0
70	168918.0
71	167807.5
72	179574.0
73	161322.5
74	119760.0
75	103052.0
76	100516.0
77	75153.5
78	53282.0
79	53982.0
80	54489.5
81	37143.5
82	21163.0
83	19239.0
84	17510.5
85	13635.5
86	9870.0
87	6095.5
88	2996.5
89	2106.5
90	1557.0
91	1158.0
92	863.5
93	697.5
94	402.5
95	112.0
96	76.0
97	68.0
98	103.0
99	137.5
100	137.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	40869.0
25	28801.0
26	37275.0
27	78254.0
28	103191.0
29	159193.0
30	25475.0
31	31917.0
32	25955.0
33	28321.0
34	26158.0
35	21672.0
36	23079.0
37	19797.0
38	22046.0
39	26342.0
40	31371.0
41	36451.0
42	45133.0
43	45476.0
44	44748.0
45	46933.0
46	79734.0
47	191595.0
48	829668.0
49	1536603.0
50	2775493.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	11.848684544847236
#Duplication Level	Percentage of deduplicated	Percentage of total
1	44.464569032752486	5.268466518916674
2	9.994780626883292	2.368500054857813
3	6.570605024565104	2.3355907861458043
4	5.630602001024542	2.6686090763090164
5	4.6601617047514985	2.760839298378901
6	3.886497748598355	2.762993148444055
7	3.360869433366747	2.787531719866745
8	2.826818235770097	2.6795262033009184
9	2.3964893633502067	2.555572183327655
>10	15.178137281236403	31.058836280284964
>50	0.5962794272247114	4.836943144081368
>100	0.34997385300303463	8.18306858532946
>500	0.04259055124793726	3.4112819644232997
>1k	0.03294220588712853	7.6990404586766275
>5k	0.005099839405120313	4.141298027742121
>10k+	0.003583670933327788	14.481902549914812
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA	390764	6.142591035203685	No Hit
CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGG	49422	0.7768861362403816	No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGC	42962	0.6753385574270422	No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTAGTTTAGGGAAGCAGTGGT	38793	0.6098042143817152	No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGC	32928	0.5176097020380254	No Hit
CGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGT	29578	0.46494957989798086	No Hit
ATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGC	23261	0.3656498809252462	No Hit
CAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGCGC	20550	0.32303448059042217	No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCG	18955	0.29796197467598307	No Hit
TACCTGGTTGATCCTGCCAGTAGCATATG	18366	0.2887032248430021	No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCC	18329	0.2881216055835449	No Hit
AAGCAGTGGTATCAACGCAGAGTGCAG	17898	0.2813465271828407	No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCC	15652	0.24604066618984366	No Hit
AAGCAGTGGTATCAACGCAGAGTG	15440	0.24270814502754834	No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA	15425	0.24247235343587648	Illumina Single End Adapter 1 (100% over 32bp)
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA	15363	0.24149774819029954	Illumina Single End Adapter 1 (100% over 32bp)
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA	14459	0.2272873749322099	No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA	14226	0.22362474554157402	No Hit
CGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTG	13772	0.2164881200336396	No Hit
CGAGGCCCTGTAATTGGAATGAGTCCACTTTAAATCCTTTAACGAGGATC	13654	0.21463322617915445	No Hit
TCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGC	12758	0.20054860843662314	No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGAC	11581	0.182046828210106	No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGA	11039	0.17352689203103017	No Hit
TCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA	10828	0.17021009030817963	Illumina Single End Adapter 1 (100% over 31bp)
ATCCGAATTCAGTCTTCCTGAGGCTCTGGCTCACCTCCAGGCAGGTCCC	10490	0.16489691977584078	No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTG	10256	0.16121857094576006	No Hit
CGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGGGGAATCA	9560	0.15027784109218667	No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCT	9474	0.14892596929993476	No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCCG	9055	0.142339524172568	No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA	8996	0.14141207724532545	No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTAG	8625	0.13558016521130856	No Hit
AACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTT	8139	0.12794051764114092	No Hit
TCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA	8090	0.12717026510834623	Illumina Single End Adapter 1 (100% over 31bp)
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG	7964	0.12518961573830278	No Hit
CAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGG	7840	0.12324040524714888	No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTAG	7775	0.12221864168323758	No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAAG	7520	0.11821018462481628	No Hit
AGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA	7344	0.11544356328253334	Illumina Single End Adapter 2 (100% over 34bp)
CCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGG	7303	0.11479906626529698	No Hit
AGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTCGCT	7303	0.11479906626529698	No Hit
ACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTCG	7248	0.11393449709583357	No Hit
CTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAATCCT	7196	0.11311708624470451	No Hit
ATCAGATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGG	7066	0.11107355911688188	No Hit
AGGCCTGGTTTGCGCTGGAGAGTGGTCTTGGTTGTGAGGGTCATAAGAT	6922	0.10880995983683223	No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGGTTGTGAGGGTTCCTGCTT	6784	0.10664067719345129	No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA	6583	0.10348106986504861	Illumina Single End Adapter 1 (100% over 32bp)
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATC	6483	0.10190912592056968	No Hit
ACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTAC	6420	0.10091880123554793	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.48181653842223987	0.0	0.0	0.0	0.0
2	0.733500483372763	0.0	0.0	0.0	0.0
3	0.8111230753511329	0.0	0.0	0.0	0.0
4	0.9122305098600184	0.0	0.0	0.0	0.0
5	1.0501528715486006	0.0	0.0	0.0	0.0
6	1.1873992973410568	0.0	0.0	0.0	0.0
7	1.2943858022022934	0.0	0.0	0.0	0.0
8	1.4046891087863806	0.0	0.0	0.0	0.0
9	1.4862887189442824	0.0	0.0	0.0	0.0
10	1.5603272787292406	0.0	0.0	0.0	0.0
11	1.6464383680077967	0.0	0.0	0.0	0.0
12	1.704333063482956	0.0	0.0	0.0	0.0
13	1.767933915476574	0.0	0.0	0.0	0.0
14	1.836172002106405	0.0	0.0	0.0	0.0
15	1.9098018564657984	0.0	0.0	0.0	0.0
16	1.9592709323985507	0.0	0.0	0.0	0.0
17	2.051795552970581	0.0	0.0	0.0	0.0
18	2.0943795144265156	0.0	0.0	0.0	0.0
19	2.19033097279751	0.0	0.0	0.0	0.0
20	2.2200721522270515	0.0	0.0	0.0	0.0
21	2.2680793202914384	0.0	0.0	0.0	0.0
22	2.2911082990780547	0.0	0.0	0.0	0.0
23	2.325895418569374	0.0	0.0	0.0	0.0
24	2.359535018981223	0.0	0.0	0.0	0.0
25	2.3865252965079264	0.0	0.0	0.0	0.0
26	2.4436340200108466	0.0	0.0	0.0	0.0
27	2.467008826465248	0.0	0.0	0.0	0.0
28	2.487491256061809	0.0	0.0	0.0	0.0
29	2.5166979745502274	0.0	0.0	0.0	0.0
30	2.532637486147244	0.0	0.0	0.0	0.0
31	2.547791025772021	0.0	0.0	0.0	1.57194394447894E-5
32	2.5609481965873098	0.0	0.0	0.0	1.57194394447894E-5
33	2.5736495036586997	0.0	0.0	0.0	1.57194394447894E-5
34	2.588693007207363	0.0	0.0	0.0	1.57194394447894E-5
35	2.6029190999048972	0.0	0.0	0.0	1.57194394447894E-5
36	2.617129473162987	0.0	0.0	0.0	1.57194394447894E-5
37	2.6305224355699477	0.0	0.0	0.0	1.57194394447894E-5
38	2.641824712530751	0.0	0.0	0.0	1.57194394447894E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAAGCCA	43465	0.0	89.064026	44
TTAAGCC	43865	0.0	57.995415	43
CGACGGG	10000	0.0	49.330624	44
ATTAAGC	43800	0.0	48.74377	42
GATTAAG	44710	0.0	46.6272	41
AGATTAA	44385	0.0	46.007984	40
AAGATTA	45150	0.0	45.00784	39
AAAGATT	45065	0.0	44.7493	38
CAAAGAT	45185	0.0	44.296856	37
CAATGAA	3305	0.0	44.23861	43
ATTAGTC	520	0.0	44.061256	44
GTCTCAA	44570	0.0	43.756832	33
CTCAAAG	45080	0.0	43.674664	35
TCAAAGA	45445	0.0	43.55174	36
TCTCAAA	45180	0.0	43.22194	34
TTGTCTC	44900	0.0	43.00407	31
TGTCTCA	45100	0.0	42.96414	32
GCTTGTC	44800	0.0	42.956318	29
ATGCTTG	44415	0.0	42.901573	27
TATGCTT	44250	0.0	42.87211	26
>>END_MODULE