FastQCFastQC Report
Tue 24 May 2016
ERR841161.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841161.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences595220
Sequences flagged as poor quality0
Sequence length24-50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC93081.5637915392627937No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA67951.1415947044790162No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA57680.9690534592251604No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGA26270.4413494170222775No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA25520.4287490339706327Illumina Single End Adapter 1 (100% over 32bp)
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA25290.42488491650146165Illumina Single End Adapter 1 (100% over 32bp)
AGGCCTGGTTTGCGCTGGAGAGTGGTCTTGGTTGTGAGGGTCATAAGAT20160.3386982964282114No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAAG19830.3331541278854877No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA18160.3050972749571587No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGAC14760.2479755384563691No Hit
TCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA12950.21756661402506636Illumina Single End Adapter 1 (100% over 31bp)
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA12420.20866234333523742Illumina Single End Adapter 1 (100% over 32bp)
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG12250.2058062565101979No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC11880.19959006753805317No Hit
CTTCCGCCACGGCCGTCTCTGGAGAGCAGCAGCCATGGCCCTACGCTAC11110.18665367427169788No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGC10980.18446960787607944No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAAT10950.18396559255401365No Hit
CCCTTCCACCTTCTGCCGTGTTAGGATTTGGTGTTCCTCCCTTCCAGA10420.17506132186418466No Hit
TCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA9830.16514902053022412Illumina Single End Adapter 1 (100% over 31bp)
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA9460.15893283155807936Illumina Single End Adapter 1 (100% over 32bp)
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA9360.15725278048452673Illumina Single End Adapter 1 (100% over 32bp)
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCC8680.1458284331843688No Hit
CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGG8110.13625214206511876No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT7620.12801989180471085No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG7550.126843856053224Illumina Single End Adapter 1 (100% over 32bp)
CGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAATGG7220.12129968751050033No Hit
ATCCGAATTCAGTCTTCCTGAGGCTCTGGCTCACCTCCAGGCAGGTCCC7020.11793958536339504No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGGTTGTGAGGGTTCCTGCTT7010.11777158025603979No Hit
CAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGCGC6790.11407546789422397No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA6600.11088337085447399No Hit
AACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTT6440.10819528913678976No Hit
TCTGGAAGAGGTCTTGCGAAAGCCGCCTCGAGCGCGCTCCGCGGCTGC6190.10399516145290816No Hit
ATTAGGCCTGGTTTGCGCTGGAGAGTGGTCTTGGTTGTGAGGGTCATA6170.10365915123819763No Hit
CTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAATCCT6040.10147508484257921No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGT6000.10080306441315817No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTAGTC11700.092.20074544
TATTAGT11800.057.32134643
TAAGCCA3200.055.93287744
CTATCAT1500.054.824344
CAATGAA10550.052.1452243
ATATTAG12050.047.90219542
AATGAAG4500.046.22440744
CGATCTT206.5294455E-445.67961534
GCGATAA206.8206683E-445.2769132
CGCGATA206.936843E-445.12198331
CATATTA12600.044.2267441
TAAGCAT12100.044.10228737
TAACCCG207.787847E-444.07443626
TCGTGCG2050.043.9929743
AAGCATA12200.043.9622938
GCAATGA10650.043.739742
GCATATT12600.043.3674240
TTAAGCA12350.042.3389936
TTTGGAC3350.042.1763443
CGCTGAA12150.042.1121828
Produced by FastQC (version 0.11.5)