##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841161.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	595220
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.12941265414469	39.0	37.0	39.0	32.0	39.0
2	37.81299351500286	39.0	37.0	39.0	35.0	39.0
3	38.03196297167434	39.0	38.0	39.0	35.0	39.0
4	38.25723766002486	39.0	38.0	40.0	35.0	41.0
5	39.006078424784114	40.0	38.0	41.0	35.0	41.0
6	38.96268438560532	40.0	38.0	41.0	35.0	41.0
7	39.00742918584725	40.0	38.0	41.0	35.0	41.0
8	38.966194012297976	40.0	38.0	41.0	35.0	41.0
9	38.88799771513054	40.0	38.0	41.0	35.0	41.0
10	38.85179093444441	40.0	38.0	41.0	35.0	41.0
11	38.8369964046907	40.0	38.0	41.0	35.0	41.0
12	38.7693558684184	40.0	38.0	41.0	35.0	41.0
13	38.723967608615304	40.0	38.0	41.0	35.0	41.0
14	38.61246597896576	40.0	38.0	41.0	35.0	41.0
15	38.56290951244918	40.0	37.0	41.0	35.0	41.0
16	38.48929471455932	40.0	37.0	41.0	35.0	41.0
17	38.408778266859315	40.0	37.0	41.0	35.0	41.0
18	38.326202076543126	40.0	37.0	41.0	35.0	41.0
19	38.22774436342865	40.0	37.0	41.0	34.0	41.0
20	38.06254662141729	39.0	36.0	41.0	34.0	41.0
21	37.805512247572324	39.0	36.0	41.0	34.0	41.0
22	37.68719633076846	39.0	35.0	41.0	33.0	41.0
23	37.49228016531703	39.0	35.0	40.0	33.0	41.0
24	37.42854238768859	39.0	35.0	40.0	33.0	41.0
25	37.28468219315929	38.0	35.0	40.0	33.0	41.0
26	37.11620906098262	38.0	35.0	40.0	33.0	41.0
27	37.06923155667781	38.0	35.0	40.0	33.0	41.0
28	37.144102658667855	38.0	35.0	40.0	33.0	41.0
29	37.16602179253979	38.0	35.0	40.0	33.0	41.0
30	37.20652679063274	38.0	35.0	40.0	33.0	41.0
31	37.144620582588615	38.0	35.0	41.0	33.0	41.0
32	37.0592899742131	37.0	35.0	41.0	33.0	41.0
33	36.93718519487266	37.0	35.0	40.0	33.0	41.0
34	36.93227347427902	37.0	35.0	40.0	34.0	41.0
35	36.811181836848434	37.0	35.0	40.0	33.0	41.0
36	36.7364551154836	36.0	35.0	40.0	33.0	41.0
37	36.62563539303301	36.0	35.0	40.0	33.0	41.0
38	36.496979000573575	36.0	35.0	40.0	33.0	41.0
39	36.36813498052807	36.0	35.0	39.0	33.0	41.0
40	36.14945580931978	35.0	35.0	39.0	33.0	41.0
41	35.99004914190101	35.0	35.0	39.0	33.0	41.0
42	35.92939804261269	35.0	35.0	39.0	33.0	41.0
43	35.85885090305934	35.0	35.0	39.0	33.0	41.0
44	35.78152342538008	35.0	35.0	38.0	33.0	41.0
45	35.72142339211689	35.0	35.0	38.0	33.0	41.0
46	35.64643523246598	35.0	35.0	37.0	33.0	41.0
47	35.56163935937967	35.0	35.0	37.0	33.0	40.0
48	35.494302573697354	35.0	35.0	37.0	33.0	40.0
49	35.531724062664324	35.0	35.0	37.0	33.0	40.0
50	35.882960935697135	36.0	35.0	39.0	33.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	14.0
14	80.0
15	175.0
16	20.0
17	0.0
18	0.0
19	0.0
20	1.0
21	0.0
22	7.0
23	14.0
24	35.0
25	126.0
26	287.0
27	575.0
28	1205.0
29	2436.0
30	4388.0
31	7328.0
32	12489.0
33	21253.0
34	43264.0
35	51953.0
36	78534.0
37	95468.0
38	98658.0
39	105928.0
40	70982.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	51.61133698464433	14.511105137596184	33.877557877759486
2	31.97036389906253	21.56244749840395	27.514532441786233	18.952656160747285
3	31.084976983300294	15.20110211350425	22.434730015792482	31.279190887402976
4	27.555021672658846	17.41204932629952	28.88663015355667	26.14629884748496
5	22.90413628574309	23.67897584086556	26.977252108464096	26.439635764927257
6	24.582003292900104	27.271765061657877	24.091428379422737	24.054803266019288
7	31.87611303383623	24.30093074829475	20.6064984375525	23.21645778031652
8	30.236047175834145	21.417459090756356	23.664359396525654	24.68213433688384
9	25.29804106044824	23.226370081650483	24.879204327811564	26.59638453008972
10	29.54437014885253	21.564127549477504	24.627700682100738	24.263801619569232
11	29.71489533281812	19.465071738180843	26.632673633278454	24.18735929572259
12	27.532844998487953	21.631161587312253	27.633480057793758	23.202513356406033
13	25.812640704277413	17.958737945633548	28.6003494506233	27.628271899465744
14	23.245858674103694	22.780988542051677	24.93935015624475	29.03380262759988
15	22.022781492557375	24.74429622660529	27.686905681932732	25.546016598904608
16	27.790396828063574	27.163233762306376	23.783643022747892	21.26272638688216
17	27.4113773058701	27.021269446591177	20.89697926816975	24.67037397936897
18	26.89207351903498	22.11199220456302	22.93992137360976	28.056012902792244
19	27.68656967171802	20.602466314975977	24.40156580760055	27.309398205705453
20	29.095796512213973	20.385067706058262	23.797587446658376	26.721548335069382
21	28.979032962602062	20.816840831961294	24.86845200094083	25.335674204495817
22	29.383757266220893	21.950875306609323	24.414166190652196	24.251201236517588
23	26.83495178253419	20.937300493935016	23.709888780618932	28.517858942911868
24	29.157118376398643	20.2760323913847	24.256241389738246	26.31060784247841
25	25.102401237256828	23.389016644210994	27.818186729880225	23.69039538865195
26	26.642436659108213	21.244786958534803	24.89014241206729	27.22263397028969
27	27.465536067699446	20.37006073841534	26.41250938374394	25.75189381014127
28	27.92161350307538	20.257025814601562	24.81796733493838	27.003393347384673
29	29.33003185818377	22.21083178248115	24.406382851955826	24.05275350737925
30	25.604383970658688	22.72175100439079	25.077202168299827	26.596662856650692
31	27.18704862349301	20.84643563393523	29.418131783029505	22.548383959542257
32	29.878081859839345	22.52664585872541	23.64184783198371	23.953424449451532
33	25.8808186159382	24.35050014622034	24.744296044421922	25.02438519341954
34	23.802140997977197	27.129461248447328	24.749703145833372	24.318694607742103
35	23.828319791147223	25.342099087735765	26.9832947112141	23.846286409902905
36	25.717515289196864	24.231490038549765	26.61461650901888	23.436378163234497
37	23.991183546070964	25.13170132783045	24.368767416102234	26.508347709996354
38	25.024826953811658	28.322217664168814	23.53372474843567	23.11923063358386
39	26.216669461680365	28.671995826112884	21.959490934838914	23.15184377736784
40	26.123587089235283	26.885940929281425	22.44065587523689	24.549816106246393
41	23.30622489203102	27.642130889302592	23.285874718300835	25.765769500365547
42	26.761724567642318	27.672031245250906	22.6596456034771	22.906598583629677
43	23.600296412335666	24.719713724044723	26.696307900233446	24.983681963386164
44	23.723045299410487	27.261092150170647	23.791498603785293	25.22436394663357
45	25.197253596689933	24.546223890894378	26.375986756597758	23.88053575581793
46	24.878847981325436	25.55075372525206	26.32104003438391	23.24935825903859
47	30.29134232372216	24.478328244749203	22.346535586343215	22.883793845185423
48	25.678570460153477	27.875568126117216	22.105763755483107	24.340097658246197
49	23.87117897431318	27.174797352938256	24.66118006568047	24.29284360706809
50	23.264089909839154	26.35231151038651	24.089585956445873	26.29401262332846
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	648.0
1	660.0
2	723.5
3	788.5
4	799.0
5	675.5
6	518.0
7	456.0
8	441.0
9	438.5
10	434.5
11	447.0
12	453.5
13	431.0
14	416.0
15	492.0
16	589.0
17	797.5
18	1003.0
19	1197.5
20	1383.0
21	1744.5
22	2176.0
23	2814.0
24	3450.0
25	3960.0
26	4705.5
27	5523.0
28	6164.5
29	6856.0
30	7639.0
31	8809.5
32	10296.5
33	11565.0
34	12561.5
35	13259.5
36	14367.5
37	16258.0
38	20027.5
39	25418.5
40	29765.0
41	32009.0
42	35587.0
43	39732.0
44	37203.0
45	31640.0
46	29758.5
47	30242.0
48	32194.5
49	35163.0
50	41896.5
51	51671.5
52	55716.5
53	50771.0
54	43849.0
55	38644.0
56	35676.5
57	35760.5
58	36916.0
59	36385.5
60	33691.5
61	31040.0
62	29658.5
63	28399.5
64	26785.0
65	25934.5
66	24887.0
67	22775.5
68	19345.0
69	16801.0
70	15009.0
71	12791.5
72	11348.0
73	9982.5
74	8141.0
75	6748.5
76	5876.0
77	4550.0
78	3489.5
79	3170.5
80	2778.5
81	1860.5
82	1181.0
83	1091.5
84	966.5
85	742.0
86	516.0
87	331.5
88	207.0
89	148.0
90	90.0
91	40.0
92	20.0
93	14.0
94	9.5
95	5.5
96	5.0
97	5.0
98	5.5
99	5.5
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	2942.0
25	2651.0
26	3507.0
27	5867.0
28	9599.0
29	13351.0
30	2057.0
31	2252.0
32	2455.0
33	2290.0
34	2793.0
35	2262.0
36	2387.0
37	2078.0
38	2059.0
39	2666.0
40	3296.0
41	4292.0
42	5520.0
43	5216.0
44	4029.0
45	4437.0
46	8385.0
47	19402.0
48	76263.0
49	156161.0
50	247003.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.87573757837949
#Duplication Level	Percentage of deduplicated	Percentage of total
1	56.28405803019039	11.18687167254347
2	9.157394996890002	3.640199597195019
3	5.440566138726552	3.24405794553439
4	4.290440083480058	3.411026447800409
5	3.5636872249590184	3.5415456047354446
6	2.9454799375400276	3.5126151768556326
7	2.563121251605355	3.566074776493294
8	2.0719204383062735	3.2944757532045204
9	1.8128141533923752	3.2427916552079132
>10	11.280786745920086	38.800926030225
>50	0.36901806593597836	4.871637998235264
>100	0.18668999080449025	7.134584592589209
>500	0.018320045826608854	2.5483507851674143
>1k	0.013085747019006324	4.212572792110112
>5k	0.002617149403801265	3.7922691721029134
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC	9308	1.5637915392627937	No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA	6795	1.1415947044790162	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA	5768	0.9690534592251604	No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGA	2627	0.4413494170222775	No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA	2552	0.4287490339706327	Illumina Single End Adapter 1 (100% over 32bp)
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA	2529	0.42488491650146165	Illumina Single End Adapter 1 (100% over 32bp)
AGGCCTGGTTTGCGCTGGAGAGTGGTCTTGGTTGTGAGGGTCATAAGAT	2016	0.3386982964282114	No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAAG	1983	0.3331541278854877	No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA	1816	0.3050972749571587	No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGAC	1476	0.2479755384563691	No Hit
TCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA	1295	0.21756661402506636	Illumina Single End Adapter 1 (100% over 31bp)
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA	1242	0.20866234333523742	Illumina Single End Adapter 1 (100% over 32bp)
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG	1225	0.2058062565101979	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC	1188	0.19959006753805317	No Hit
CTTCCGCCACGGCCGTCTCTGGAGAGCAGCAGCCATGGCCCTACGCTAC	1111	0.18665367427169788	No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGC	1098	0.18446960787607944	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAAT	1095	0.18396559255401365	No Hit
CCCTTCCACCTTCTGCCGTGTTAGGATTTGGTGTTCCTCCCTTCCAGA	1042	0.17506132186418466	No Hit
TCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA	983	0.16514902053022412	Illumina Single End Adapter 1 (100% over 31bp)
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA	946	0.15893283155807936	Illumina Single End Adapter 1 (100% over 32bp)
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA	936	0.15725278048452673	Illumina Single End Adapter 1 (100% over 32bp)
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCC	868	0.1458284331843688	No Hit
CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGG	811	0.13625214206511876	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT	762	0.12801989180471085	No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG	755	0.126843856053224	Illumina Single End Adapter 1 (100% over 32bp)
CGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAATGG	722	0.12129968751050033	No Hit
ATCCGAATTCAGTCTTCCTGAGGCTCTGGCTCACCTCCAGGCAGGTCCC	702	0.11793958536339504	No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGGTTGTGAGGGTTCCTGCTT	701	0.11777158025603979	No Hit
CAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGCGC	679	0.11407546789422397	No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA	660	0.11088337085447399	No Hit
AACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTT	644	0.10819528913678976	No Hit
TCTGGAAGAGGTCTTGCGAAAGCCGCCTCGAGCGCGCTCCGCGGCTGC	619	0.10399516145290816	No Hit
ATTAGGCCTGGTTTGCGCTGGAGAGTGGTCTTGGTTGTGAGGGTCATA	617	0.10365915123819763	No Hit
CTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAATCCT	604	0.10147508484257921	No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGT	600	0.10080306441315817	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.19354188367326366	0.0	0.0	0.0	0.0
2	0.30022512684385605	0.0	0.0	0.0	0.0
3	0.37515540472430364	0.0	0.0	0.0	0.0
4	0.49813514330835656	0.0	0.0	0.0	0.0
5	0.6249789993615806	0.0	0.0	0.0	0.0
6	0.7916400658580021	0.0	0.0	0.0	0.0
7	0.9112597022949498	0.0	0.0	0.0	0.0
8	1.0092066798830683	0.0	0.0	0.0	0.0
9	1.088001075232687	0.0	0.0	0.0	0.0
10	1.1562111488189242	0.0	0.0	0.0	0.0
11	1.2242532172978058	0.0	0.0	0.0	0.0
12	1.2855750814824771	0.0	0.0	0.0	0.0
13	1.3388327005140956	0.0	0.0	0.0	0.0
14	1.3922583246530695	0.0	0.0	0.0	0.0
15	1.445515943684688	0.0	0.0	0.0	0.0
16	1.4844931285911092	0.0	0.0	0.0	0.0
17	1.5333826148314909	0.0	0.0	0.0	0.0
18	1.566647626087833	0.0	0.0	0.0	0.0
19	1.6141930714693726	0.0	0.0	0.0	0.0
20	1.6432579550418334	0.0	0.0	0.0	0.0
21	1.6721548335069387	0.0	0.0	0.0	0.0
22	1.6919794361748597	0.0	0.0	0.0	0.0
23	1.7158361614193072	0.0	0.0	0.0	0.0
24	1.7400288968784652	0.0	0.0	0.0	0.0
25	1.758509458687544	0.0	0.0	0.0	0.0
26	1.783374214576123	0.0	0.0	0.0	0.0
27	1.8018547763852022	0.0	0.0	0.0	0.0
28	1.8188232922280838	0.0	0.0	0.0	0.0
29	1.8376398642518732	0.0	0.0	0.0	0.0
30	1.8512482779476496	0.0	0.0	0.0	0.0
31	1.8683847988978866	0.0	0.0	0.0	0.0
32	1.882665233023084	0.0	0.0	0.0	0.0
33	1.8962736467188603	0.0	0.0	0.0	0.0
34	1.9102180706293472	0.0	0.0	0.0	0.0
35	1.9223144383589261	0.0	0.0	0.0	0.0
36	1.9364268673767682	0.0	0.0	0.0	0.0
37	1.9468431840327947	0.0	0.0	0.0	0.0
38	1.9587715466550184	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTAGTC	1170	0.0	92.200745	44
TATTAGT	1180	0.0	57.321346	43
TAAGCCA	320	0.0	55.932877	44
CTATCAT	150	0.0	54.8243	44
CAATGAA	1055	0.0	52.14522	43
ATATTAG	1205	0.0	47.902195	42
AATGAAG	450	0.0	46.224407	44
CGATCTT	20	6.5294455E-4	45.679615	34
GCGATAA	20	6.8206683E-4	45.27691	32
CGCGATA	20	6.936843E-4	45.121983	31
CATATTA	1260	0.0	44.22674	41
TAAGCAT	1210	0.0	44.102287	37
TAACCCG	20	7.787847E-4	44.074436	26
TCGTGCG	205	0.0	43.99297	43
AAGCATA	1220	0.0	43.96229	38
GCAATGA	1065	0.0	43.7397	42
GCATATT	1260	0.0	43.36742	40
TTAAGCA	1235	0.0	42.33899	36
TTTGGAC	335	0.0	42.17634	43
CGCTGAA	1215	0.0	42.11218	28
>>END_MODULE