FastQCFastQC Report
Tue 24 May 2016
ERR841160.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841160.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2809345
Sequences flagged as poor quality0
Sequence length24-50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA1134814.0394113218561625No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA213880.761316249873191No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGC201050.7156472416168181No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC196010.6977071167834495No Hit
CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGG177770.6327809507198298No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGC135240.48139334969539166No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGC120470.428818817197603No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA105070.37400176909564326No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCG91950.32730049175163606No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTGCTGTGGGGAAGCAGTGGT88730.31583874533031725No Hit
AAGCAGTGGTATCAACGCAGAGTG86640.3083992888021941No Hit
ATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGC86270.30708225582831583No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA84270.2999631586722172Illumina Single End Adapter 1 (100% over 32bp)
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGAC83760.298147788897412No Hit
CGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGT81930.29163381499958174No Hit
CAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGCGC79760.2839095945852147No Hit
AAGCAGTGGTATCAACGCAGAGTGCAG79390.28259256161133645No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA77950.27746681165894543Illumina Single End Adapter 1 (100% over 32bp)
AGGCCTGGTTTGCGCTGGAGAGTGGTCTTGGTTGTGAGGGTCATAAGAT77800.276932879372238No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAAG71120.25315509487086846No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCC68620.24425622342574516No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGA67170.2390948779875736No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAAT60770.21631376708805788No Hit
AACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTT56890.20250271860522648No Hit
AAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCGAATG56740.20196878631851908No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCC56710.2018619998611776No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCCG55760.19848042871203073No Hit
CAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGG53750.19132573607015158No Hit
TACCTGGTTGATCCTGCCAGTAGCATATG52460.18673391840446793No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTG50400.17940124833368631No Hit
ATCAGATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGG50120.1784045747318325No Hit
ATCCGAATTCAGTCTTCCTGAGGCTCTGGCTCACCTCCAGGCAGGTCCC48240.17171262340509977No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG45890.16334768424668383No Hit
TCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA45720.16274256098841544Illumina Single End Adapter 1 (100% over 31bp)
ACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTCG42120.14992818610743786No Hit
AGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTCGCT41930.1492518718776085No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCT41550.14789924341794974No Hit
TCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGC38310.13636630602506988No Hit
CTCTGCAGGTACCATGGAGCTGAGCTATAGGCTCTTCATCTGCCTCCT37910.1349424865938502No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA36340.1293539953263127Illumina Single End Adapter 1 (100% over 32bp)
CTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAATCCT35850.12760981652306855No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAAT35600.12671992937855622No Hit
TCTGGAAGAGGTCTTGCGAAAGCCGCCTCGAGCGCGCTCCGCGGCTGC35400.12600801966294634No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGT34840.12401467245923871No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTG34070.12127382005414074No Hit
ATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAAT33070.1177142714760914No Hit
CGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGGGGAATCA33010.11750069856140843No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA32610.1160768791301887No Hit
CCCTTCCACCTTCTGCCGTGTTAGGATTTGGTGTTCCTCCCTTCCAGA32500.11568532878660329No Hit
CTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAATCCTG32340.1151158010141154No Hit
CTTCCGCCACGGCCGTCTCTGGAGAGCAGCAGCCATGGCCCTACGCTAC32000.11390555449757862No Hit
TCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA31580.11241054409479789Illumina Single End Adapter 1 (100% over 31bp)
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTAG31170.11095112917779766No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTGC30270.10774753545755328No Hit
ATTAGGCCTGGTTTGCGCTGGAGAGTGGTCTTGGTTGTGAGGGTCATA29950.10660847991257748No Hit
CGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTG29830.10618133408321158No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGCCA128500.087.3286244
ATTAGTC23050.086.00957544
CTATACG2400.056.9013344
TTAAGCC128100.056.25936543
TATTAGT23350.054.5497443
CAATGAA39150.049.87642343
ATTAAGC128600.047.53428342
CGACGGG41450.046.0114744
GATTAAG132200.045.24831441
AGATTAA130750.044.94459540
ATATTAG24150.044.79858842
CATATTA23500.044.59501341
AAGATTA132800.044.05012539
AATCCCG7500.043.6595243
AAAGATT134350.043.35417638
GCATATT23900.043.27145440
GTCTCAA131450.042.92611333
CAAAGAT135200.042.83661337
GCAATGA38850.042.62146842
AATGAAG17850.042.47401444
Produced by FastQC (version 0.11.5)