FastQCFastQC Report
Tue 24 May 2016
ERR841159.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841159.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7356897
Sequences flagged as poor quality0
Sequence length24-50
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA919641.2500378896156898No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCC684720.9307184809030221No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGA426520.5797552962886391No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTATGCTTGGGAAGCAGTGGT402800.5475134421482318No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGAC397390.5401597983497662No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCCG390620.530957549086252No Hit
ATCCGAATTCAGTCTTCCTGAGGCTCTGGCTCACCTCCAGGCAGGTCCC323160.4392612809449419No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA322250.4380243464058285No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAAT285410.3879488866025989No Hit
CTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAATCCT254550.3460018537706862No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATC233780.3177698423669653No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA206710.2809744380001514No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA147620.2006552490812363No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA146300.19886101436515968Illumina Single End Adapter 1 (100% over 32bp)
CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGG144520.19644151603590482No Hit
CTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAATCCTG144270.1961016988548297No Hit
ACTACTTCTCCCCCGGACTCCTTGGTAGTCTGTTAGTGGGAGATCCTTG142950.19430746413875308No Hit
CTGCGCGTCTGCTCCTCCTTCTCACGTTTCTCCTGCCGCCCTAATCCT138110.18772860351313875No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA133290.18117692826201046Illumina Single End Adapter 1 (100% over 32bp)
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGC129370.1758485948627526No Hit
AAGCAGTGGTATCAACGCAGAGTGCAG124070.16864447062396007No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTAG123870.1683726168791No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG122070.1659259331753591No Hit
AAGCAGTGGTATCAACGCAGAGTG120000.16311224691605714No Hit
ATCCGAATTCAGTCTTCCTGAGGCTCTGGCTCACCTCCAGGCAGGTCCCA107360.14593109024089912No Hit
TCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA103750.1410241301461744Illumina Single End Adapter 1 (100% over 31bp)
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAAG100600.13674243366462788No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGC98150.13341222529009172No Hit
AGCGAGCGGCCTTGAGAGGCTCTGGCTCTTGCTTCTTAGGCGGCCCGAG92860.12622169373854222No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAAT92770.12609935955335516No Hit
AGGCCTGGTTTGCGCTGGAGAGTGGTCTTGGTTGTGAGGGTCATAAGAT92560.1258139131212521No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGGTTGTGAGGGTTCCTGCTT87610.11908553293596472No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTG87280.11863697425694557No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTA84210.1144640192733431No Hit
CTGCGCGTCTGCTCCTCCTTCTCACGTTTCTCCTGCCGCCCTAATCCTG81890.111310515832966No Hit
TCTGGAAGAGGTCTTGCGAAAGCCGCCTCGAGCGCGCTCCGCGGCTGC81620.11094351327740487No Hit
CTCTCTCCTCCCGCCGCCCAAGATGCCGAAAGGAAAGAAGGCCAAGGGA80190.10899975900165518No Hit
ATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGC77860.10583266287403506No Hit
AGCTGTTTTTCGTCTTCCCTAGGCTATTTCTGCCGGGCGCTCCGCGAAG77630.10552003106744595No Hit
TCGCTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAAT76750.10432387459006155No Hit
ACCAGCGAGCGGCCTTGAGAGGCTCTGGCTCTTGCTTCTTAGGCGGCCC73720.1002052903554311No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGCCA106250.0136.9904344
CGACGGG35550.069.7499244
TTAAGCC103400.061.62855543
CCAGAAT26600.058.0089844
AATCCCG55550.052.34776343
TTTGGAC55550.051.16208643
AGGTCCC59550.051.0436243
AATCCTG52000.048.70174443
ATTAAGC102700.047.59177442
GGTCCCA33800.047.01478644
CAATGAA48300.046.2278943
ATGTTCT13150.046.11924444
AATGAAG35250.045.7341944
TCGTGCG25400.045.6384143
TAATCCT106650.045.04753542
GATTAAG108000.044.1225441
TAATCCC136900.044.06078742
AGATTAA105700.043.87391340
CTAATCC242650.043.37998641
ATCCTTG28100.043.1283743
Produced by FastQC (version 0.11.5)