##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841159.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7356897
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.427796392962954	39.0	37.0	39.0	34.0	39.0
2	37.98445662621075	39.0	38.0	39.0	35.0	39.0
3	38.33850195265749	39.0	38.0	39.0	35.0	41.0
4	38.8300566121831	39.0	39.0	41.0	35.0	41.0
5	39.327341269016	41.0	39.0	41.0	36.0	41.0
6	39.32300112397931	41.0	39.0	41.0	35.0	41.0
7	39.27559839426867	41.0	39.0	41.0	35.0	41.0
8	39.32159319343468	40.0	39.0	41.0	35.0	41.0
9	39.318462253855124	40.0	39.0	41.0	35.0	41.0
10	39.18619412505028	40.0	39.0	41.0	35.0	41.0
11	39.2620661944839	40.0	39.0	41.0	35.0	41.0
12	39.21083522033814	40.0	39.0	41.0	35.0	41.0
13	39.207942152785336	40.0	39.0	41.0	35.0	41.0
14	39.19327686659199	40.0	39.0	41.0	35.0	41.0
15	39.19557919595721	40.0	39.0	41.0	35.0	41.0
16	39.12620823697817	40.0	38.0	41.0	35.0	41.0
17	39.03837569018569	40.0	38.0	41.0	35.0	41.0
18	38.99194116758737	40.0	38.0	41.0	35.0	41.0
19	38.894064304556665	40.0	38.0	41.0	35.0	41.0
20	38.83147120314448	40.0	38.0	41.0	35.0	41.0
21	38.47267115470014	40.0	38.0	41.0	34.0	41.0
22	38.46217161936615	40.0	38.0	41.0	34.0	41.0
23	38.28303644865492	40.0	37.0	41.0	34.0	41.0
24	38.15367144599143	40.0	37.0	41.0	34.0	41.0
25	38.118695838836615	40.0	37.0	41.0	34.0	41.0
26	38.06069404416255	40.0	36.0	41.0	34.0	41.0
27	38.02160387657106	40.0	36.0	41.0	34.0	41.0
28	38.05509445364095	40.0	36.0	41.0	34.0	41.0
29	38.1340088768777	40.0	36.0	41.0	34.0	41.0
30	38.17556763815807	40.0	36.0	41.0	34.0	41.0
31	38.127889939287094	40.0	35.0	41.0	34.0	41.0
32	38.080100404090715	40.0	35.0	41.0	34.0	41.0
33	37.986608547348204	40.0	35.0	41.0	34.0	41.0
34	37.90828388216089	39.0	35.0	41.0	34.0	41.0
35	37.78819753634155	39.0	35.0	41.0	34.0	41.0
36	37.6172167923874	39.0	35.0	41.0	34.0	41.0
37	37.614564701571396	39.0	35.0	41.0	34.0	41.0
38	37.50986233280868	39.0	35.0	41.0	34.0	41.0
39	37.327302139741214	38.0	35.0	41.0	33.0	41.0
40	37.19981453880366	38.0	35.0	41.0	33.0	41.0
41	37.01878383657251	37.0	35.0	41.0	33.0	41.0
42	36.9154206314178	37.0	35.0	41.0	33.0	41.0
43	36.80292987028818	37.0	35.0	40.0	33.0	41.0
44	36.721234978316474	36.0	35.0	40.0	33.0	41.0
45	36.646479758816234	36.0	35.0	40.0	33.0	41.0
46	36.47340349156875	36.0	35.0	40.0	33.0	41.0
47	36.353186590040174	36.0	35.0	40.0	33.0	41.0
48	36.32320117523893	36.0	35.0	39.0	33.0	41.0
49	36.551989769227326	36.0	35.0	40.0	33.0	41.0
50	36.94286237942419	38.0	35.0	40.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	8.0
14	326.0
15	2122.0
16	2041.0
17	51.0
18	0.0
19	1.0
20	6.0
21	20.0
22	51.0
23	114.0
24	323.0
25	876.0
26	2073.0
27	4967.0
28	11235.0
29	23391.0
30	42172.0
31	72109.0
32	118499.0
33	197822.0
34	420338.0
35	373756.0
36	596579.0
37	857982.0
38	1246738.0
39	1623898.0
40	1759399.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	47.08176558676844	19.338356918684603	33.579877494546956
2	29.046213369576872	22.766487012119377	26.852027424062076	21.335272194241675
3	33.819380643768696	14.678158468169391	22.938896657109648	28.56356423095226
4	22.61662763526525	16.5689148563586	28.007188356721592	32.80726915165456
5	27.06342089606528	20.443646281849535	27.419073557778507	25.073859264306677
6	30.07704199202463	20.319096488641883	22.872102191997524	26.73175932733597
7	33.87625244719343	19.891579289474898	22.49820814400419	23.73396011932748
8	31.84053004955758	16.926089899043035	26.111633749935603	25.121746301463784
9	28.62115644680087	17.981113504783337	24.91686372664997	28.48086632176582
10	29.843859442370878	19.331451833565154	25.987369403160056	24.83731932090391
11	32.35782694796461	20.403874078976504	23.608445245325576	23.62985372773331
12	29.171347648335978	16.532445676485615	26.050915216021103	28.245291459157308
13	25.8807075863642	16.530433958773653	27.02513029610174	30.563728158760412
14	26.7861028909335	21.147652332226478	20.93706082877061	31.12918394806941
15	24.284423174607447	21.273642950281893	24.304649093225038	30.137284781885626
16	28.989151268530737	19.638238784639775	26.41511767801017	24.957492268819315
17	29.79356649957176	18.951373112876258	19.56168477008717	31.69337561746481
18	28.379288169998844	18.681463122291913	19.056308658392254	33.88294004931699
19	27.682866839103497	20.595816415534973	26.688684101462883	25.032632643898644
20	33.768516808105375	16.83031582472882	23.048263418666863	26.35290394849894
21	28.24419045149062	20.978749600544905	22.602912613837056	28.17414733412742
22	31.282278384487373	19.314202713453785	25.08057405180472	24.32294485025412
23	30.450134071470618	18.36827401552584	24.327335288233613	26.854256624769928
24	32.50460078481458	20.15609298322377	20.752689619006492	26.58661661295516
25	28.162779505101305	19.541839968716758	24.813722514363217	27.481658011818723
26	30.447182981547705	20.52389150646005	22.747815127043086	26.281110384949162
27	29.937354960833378	20.70420892989519	25.3903192374397	23.968116871831725
28	29.236511647852925	18.067653797233127	26.80040428479094	25.895430270123004
29	33.4987594118585	18.86963287501986	25.13234301176893	22.499264701352708
30	27.60131339004004	19.409073636573346	23.3225886697216	29.66702430366502
31	28.181577920504385	19.017260678933216	30.373787370867035	22.427374029695365
32	28.221593352252388	19.87807402205236	24.31058245758769	27.589750168107564
33	27.339763832610686	19.223579601230984	25.13712267978077	28.29953388637756
34	26.596551217055865	18.9762976012406	29.788509899394406	24.63864128230913
35	28.835405838443872	19.820572146659885	22.346630417047272	28.997391597848964
36	26.977971974693478	20.58049206861509	25.047152776194686	27.394383180496746
37	25.127547199125384	21.01291648268566	24.759971594788244	29.09956472340071
38	31.60477222083271	22.50202318406479	20.910889981857462	24.982314613245034
39	27.36993165858254	23.825823043362238	22.41354708988091	26.390698208174314
40	28.225785053492793	22.408369634137305	20.948819602464642	28.417025709905264
41	28.089437521692446	22.35254006555394	22.249965376441285	27.308057036312327
42	27.508356455689952	22.93307736704516	24.92967614297487	24.62889003429002
43	25.383543362413008	24.511199797369503	24.307387601258885	25.797869238958604
44	25.2082268208641	23.186988789081433	24.454850587635654	27.149933802418815
45	26.37022502715241	22.044700118607985	27.08586281435414	24.499212039885464
46	27.220021044286923	23.5105595519891	22.20275740649062	27.06666199723336
47	30.360202804348212	20.131116800342003	22.06697207148999	27.441708323819796
48	24.953632096180474	22.75306473945493	23.573621289385702	28.719681874978892
49	28.463757959970515	22.057345546068152	20.462516181402933	29.016380312558404
50	24.657967462436616	27.99155577893678	24.623313709897264	22.727163048729338
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	5266.0
1	5380.0
2	6043.0
3	6347.0
4	5829.5
5	4263.5
6	2586.5
7	2327.5
8	2558.5
9	2733.0
10	2767.0
11	2696.5
12	2629.5
13	2625.0
14	2669.5
15	3285.5
16	4057.5
17	5184.5
18	6333.0
19	7695.5
20	9047.5
21	11342.0
22	13783.0
23	17044.0
24	20439.0
25	22781.0
26	26439.0
27	32062.0
28	37512.5
29	43096.5
30	49540.5
31	55761.5
32	63960.5
33	73664.0
34	82631.5
35	91842.0
36	104659.0
37	118181.5
38	142308.0
39	186526.5
40	231125.0
41	265533.5
42	297568.5
43	357862.0
44	391013.0
45	341923.5
46	310758.5
47	319783.5
48	356862.0
49	423155.0
50	506671.0
51	583444.0
52	604733.5
53	576239.5
54	524518.0
55	498011.0
56	512508.5
57	567071.0
58	646518.5
59	639123.5
60	550570.0
61	495469.0
62	472216.0
63	453953.5
64	451185.0
65	453867.5
66	410335.0
67	353870.5
68	309649.5
69	273356.5
70	244331.5
71	221569.0
72	210725.5
73	188057.5
74	155775.0
75	134049.0
76	122005.0
77	96748.5
78	75729.5
79	64756.0
80	52092.0
81	36203.5
82	24827.5
83	19727.0
84	15212.5
85	12097.0
86	9068.5
87	5614.5
88	3131.0
89	2157.0
90	1493.5
91	852.5
92	507.0
93	416.0
94	264.5
95	106.5
96	65.5
97	63.5
98	50.5
99	36.5
100	36.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	43077.0
25	33534.0
26	41724.0
27	102998.0
28	155407.0
29	122179.0
30	28293.0
31	31954.0
32	33715.0
33	31786.0
34	33493.0
35	31049.0
36	25245.0
37	25398.0
38	29239.0
39	30100.0
40	37579.0
41	40107.0
42	48609.0
43	45226.0
44	45128.0
45	59067.0
46	106580.0
47	253210.0
48	1389991.0
49	2584368.0
50	1947841.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	12.012110488195143
#Duplication Level	Percentage of deduplicated	Percentage of total
1	44.62270146270102	5.360128202517117
2	9.99807188197185	2.4019588823032603
3	6.713426029303074	2.419272456549412
4	5.43458325048507	2.6112325784848545
5	4.554948883232692	2.735727462673609
6	3.861335480792404	2.7829673056359883
7	3.2049066150055214	2.6948384654655673
8	2.772131225169284	2.6639317251607366
9	2.3178155167191368	2.505767047027505
>10	15.287690014431057	32.304349831541046
>50	0.7023954584267288	5.802348785983652
>100	0.4436201906622155	10.239578230668629
>500	0.04241896990794279	3.574169795241873
>1k	0.03603839103106105	8.35224635366617
>5k	0.0047263463647274715	3.9344956088247924
>10k+	0.003190283796191043	9.616987268255905
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA	91964	1.2500378896156898	No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCC	68472	0.9307184809030221	No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGA	42652	0.5797552962886391	No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTATGCTTGGGAAGCAGTGGT	40280	0.5475134421482318	No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGAC	39739	0.5401597983497662	No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCCG	39062	0.530957549086252	No Hit
ATCCGAATTCAGTCTTCCTGAGGCTCTGGCTCACCTCCAGGCAGGTCCC	32316	0.4392612809449419	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA	32225	0.4380243464058285	No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAAT	28541	0.3879488866025989	No Hit
CTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAATCCT	25455	0.3460018537706862	No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATC	23378	0.3177698423669653	No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA	20671	0.2809744380001514	No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA	14762	0.2006552490812363	No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA	14630	0.19886101436515968	Illumina Single End Adapter 1 (100% over 32bp)
CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGG	14452	0.19644151603590482	No Hit
CTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAATCCTG	14427	0.1961016988548297	No Hit
ACTACTTCTCCCCCGGACTCCTTGGTAGTCTGTTAGTGGGAGATCCTTG	14295	0.19430746413875308	No Hit
CTGCGCGTCTGCTCCTCCTTCTCACGTTTCTCCTGCCGCCCTAATCCT	13811	0.18772860351313875	No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA	13329	0.18117692826201046	Illumina Single End Adapter 1 (100% over 32bp)
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGC	12937	0.1758485948627526	No Hit
AAGCAGTGGTATCAACGCAGAGTGCAG	12407	0.16864447062396007	No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTAG	12387	0.1683726168791	No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG	12207	0.1659259331753591	No Hit
AAGCAGTGGTATCAACGCAGAGTG	12000	0.16311224691605714	No Hit
ATCCGAATTCAGTCTTCCTGAGGCTCTGGCTCACCTCCAGGCAGGTCCCA	10736	0.14593109024089912	No Hit
TCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA	10375	0.1410241301461744	Illumina Single End Adapter 1 (100% over 31bp)
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAAG	10060	0.13674243366462788	No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGC	9815	0.13341222529009172	No Hit
AGCGAGCGGCCTTGAGAGGCTCTGGCTCTTGCTTCTTAGGCGGCCCGAG	9286	0.12622169373854222	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAAT	9277	0.12609935955335516	No Hit
AGGCCTGGTTTGCGCTGGAGAGTGGTCTTGGTTGTGAGGGTCATAAGAT	9256	0.1258139131212521	No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGGTTGTGAGGGTTCCTGCTT	8761	0.11908553293596472	No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTG	8728	0.11863697425694557	No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTA	8421	0.1144640192733431	No Hit
CTGCGCGTCTGCTCCTCCTTCTCACGTTTCTCCTGCCGCCCTAATCCTG	8189	0.111310515832966	No Hit
TCTGGAAGAGGTCTTGCGAAAGCCGCCTCGAGCGCGCTCCGCGGCTGC	8162	0.11094351327740487	No Hit
CTCTCTCCTCCCGCCGCCCAAGATGCCGAAAGGAAAGAAGGCCAAGGGA	8019	0.10899975900165518	No Hit
ATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGC	7786	0.10583266287403506	No Hit
AGCTGTTTTTCGTCTTCCCTAGGCTATTTCTGCCGGGCGCTCCGCGAAG	7763	0.10552003106744595	No Hit
TCGCTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAAT	7675	0.10432387459006155	No Hit
ACCAGCGAGCGGCCTTGAGAGGCTCTGGCTCTTGCTTCTTAGGCGGCCC	7372	0.1002052903554311	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.2789355349137007	0.0	0.0	0.0	0.0
2	0.41009137412145363	0.0	0.0	0.0	0.0
3	0.48830369651770306	0.0	0.0	0.0	0.0
4	0.576669756284477	1.3592687243004761E-5	0.0	0.0	0.0
5	0.6819723043560348	1.3592687243004761E-5	0.0	0.0	0.0
6	0.8059919827612103	1.3592687243004761E-5	0.0	0.0	0.0
7	0.9013854618326177	1.3592687243004761E-5	0.0	0.0	0.0
8	1.0051384435584731	1.3592687243004761E-5	0.0	0.0	0.0
9	1.0722863185389166	1.3592687243004761E-5	0.0	0.0	0.0
10	1.136185541268282	1.3592687243004761E-5	0.0	0.0	0.0
11	1.2034013796849405	1.3592687243004761E-5	0.0	0.0	0.0
12	1.253123429619852	1.3592687243004761E-5	0.0	0.0	0.0
13	1.3020027329456971	1.3592687243004761E-5	0.0	0.0	0.0
14	1.3566589283498192	5.4370748972019044E-5	0.0	0.0	0.0
15	1.4098471135316968	5.4370748972019044E-5	0.0	0.0	0.0
16	1.4492115357874387	5.4370748972019044E-5	0.0	0.0	0.0
17	1.5118466386032046	5.4370748972019044E-5	0.0	0.0	0.0
18	1.544428309924687	5.4370748972019044E-5	0.0	0.0	0.0
19	1.6058400708885825	5.4370748972019044E-5	0.0	0.0	0.0
20	1.6312040252840294	5.4370748972019044E-5	0.0	0.0	0.0
21	1.6661916022475236	5.4370748972019044E-5	0.0	0.0	0.0
22	1.6858058499391795	5.4370748972019044E-5	0.0	0.0	0.0
23	1.7123659608120108	5.4370748972019044E-5	0.0	0.0	0.0
24	1.7403940819070867	5.4370748972019044E-5	0.0	0.0	0.0
25	1.7621151961214083	5.4370748972019044E-5	0.0	0.0	1.3592687243004761E-5
26	1.8056253879862665	5.4370748972019044E-5	0.0	0.0	1.3592687243004761E-5
27	1.8237852181429208	5.4370748972019044E-5	0.0	0.0	1.3592687243004761E-5
28	1.8432091682131746	5.4370748972019044E-5	0.0	0.0	1.3592687243004761E-5
29	1.8677303759995552	5.4370748972019044E-5	0.0	0.0	1.3592687243004761E-5
30	1.8837697469463008	5.4370748972019044E-5	0.0	0.0	1.3592687243004761E-5
31	1.8993741519012703	5.4370748972019044E-5	0.0	0.0	1.3592687243004761E-5
32	1.912654207337686	5.4370748972019044E-5	0.0	0.0	1.3592687243004761E-5
33	1.9251730722884934	5.4370748972019044E-5	0.0	0.0	1.3592687243004761E-5
34	1.9399347306343966	5.4370748972019044E-5	0.0	0.0	1.3592687243004761E-5
35	1.9547643524165148	5.4370748972019044E-5	0.0	0.0	1.3592687243004761E-5
36	1.969797864507278	5.4370748972019044E-5	0.0	0.0	1.3592687243004761E-5
37	1.9832546248778526	5.4370748972019044E-5	0.0	0.0	1.3592687243004761E-5
38	1.9943462576681446	5.4370748972019044E-5	0.0	0.0	1.3592687243004761E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAAGCCA	10625	0.0	136.99043	44
CGACGGG	3555	0.0	69.74992	44
TTAAGCC	10340	0.0	61.628555	43
CCAGAAT	2660	0.0	58.00898	44
AATCCCG	5555	0.0	52.347763	43
TTTGGAC	5555	0.0	51.162086	43
AGGTCCC	5955	0.0	51.04362	43
AATCCTG	5200	0.0	48.701744	43
ATTAAGC	10270	0.0	47.591774	42
GGTCCCA	3380	0.0	47.014786	44
CAATGAA	4830	0.0	46.22789	43
ATGTTCT	1315	0.0	46.119244	44
AATGAAG	3525	0.0	45.73419	44
TCGTGCG	2540	0.0	45.63841	43
TAATCCT	10665	0.0	45.047535	42
GATTAAG	10800	0.0	44.12254	41
TAATCCC	13690	0.0	44.060787	42
AGATTAA	10570	0.0	43.873913	40
CTAATCC	24265	0.0	43.379986	41
ATCCTTG	2810	0.0	43.12837	43
>>END_MODULE