##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841158.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	930688
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.54857266882134	39.0	35.0	39.0	32.0	39.0
2	37.50158270011003	39.0	37.0	39.0	35.0	39.0
3	37.727115854077844	39.0	37.0	39.0	35.0	40.0
4	38.11767316221978	39.0	37.0	40.0	35.0	41.0
5	38.47522155652592	40.0	38.0	41.0	34.0	41.0
6	38.394221264269014	40.0	38.0	41.0	34.0	41.0
7	38.195040658093795	40.0	37.0	41.0	33.0	41.0
8	38.10891405068079	40.0	37.0	41.0	33.0	41.0
9	38.09120779466373	40.0	37.0	41.0	33.0	41.0
10	38.11489134919543	40.0	37.0	41.0	34.0	41.0
11	38.154002200522626	40.0	37.0	41.0	34.0	41.0
12	37.9852560686288	39.0	36.0	41.0	34.0	41.0
13	37.849515627148946	39.0	36.0	41.0	33.0	41.0
14	37.77259618690689	39.0	35.0	41.0	33.0	41.0
15	37.63886823511209	39.0	35.0	40.0	33.0	41.0
16	37.54183786618072	39.0	35.0	40.0	33.0	41.0
17	37.40269671468849	38.0	35.0	40.0	33.0	41.0
18	37.32591803053225	38.0	35.0	40.0	33.0	41.0
19	37.103886587126944	38.0	35.0	40.0	33.0	41.0
20	37.027298084857655	38.0	35.0	40.0	33.0	41.0
21	36.71348185428414	37.0	35.0	40.0	33.0	41.0
22	36.4816200488241	37.0	35.0	40.0	32.0	41.0
23	36.4066475553569	37.0	35.0	40.0	32.0	41.0
24	36.225309663388806	37.0	34.0	40.0	31.0	41.0
25	36.104716169957335	36.0	34.0	40.0	31.0	41.0
26	35.89867460726887	36.0	34.0	40.0	31.0	41.0
27	35.803887749649725	36.0	34.0	39.0	31.0	41.0
28	35.97350924012056	36.0	35.0	39.0	32.0	41.0
29	36.02630461824559	35.0	35.0	39.0	32.0	41.0
30	36.02040906306273	35.0	35.0	39.0	32.0	41.0
31	35.957559598128334	35.0	35.0	39.0	32.0	41.0
32	35.92343021742037	35.0	35.0	39.0	33.0	41.0
33	35.85847846185777	35.0	35.0	39.0	33.0	41.0
34	35.791576641868616	35.0	35.0	39.0	33.0	41.0
35	35.71042278640369	35.0	35.0	39.0	33.0	41.0
36	35.61475586663151	35.0	35.0	38.0	32.0	41.0
37	35.5634497355007	35.0	35.0	38.0	33.0	41.0
38	35.45135972194844	35.0	35.0	37.0	33.0	41.0
39	35.38499082287976	35.0	35.0	37.0	32.0	41.0
40	35.26257565658563	35.0	35.0	37.0	32.0	41.0
41	35.141488032251175	35.0	34.0	37.0	32.0	40.0
42	35.0832580987457	35.0	35.0	36.0	32.0	40.0
43	35.05116239606313	35.0	34.0	36.0	32.0	40.0
44	35.007301035751766	35.0	34.0	36.0	32.0	40.0
45	34.86809957856529	35.0	34.0	36.0	32.0	40.0
46	34.875321993021345	35.0	34.0	36.0	32.0	40.0
47	34.84928219681162	35.0	34.0	35.0	32.0	39.0
48	34.694507416106745	35.0	34.0	35.0	32.0	39.0
49	34.88318657226036	35.0	34.0	36.0	32.0	39.0
50	35.48378562894692	35.0	34.0	38.0	32.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	4.0
14	34.0
15	48.0
16	7.0
17	0.0
18	0.0
19	0.0
20	4.0
21	3.0
22	27.0
23	76.0
24	207.0
25	483.0
26	1073.0
27	2179.0
28	4258.0
29	7691.0
30	13497.0
31	21231.0
32	32604.0
33	51411.0
34	96109.0
35	125804.0
36	155758.0
37	140611.0
38	120028.0
39	95841.0
40	61700.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	40.61575952413698	18.79899601155274	40.58524446431027
2	26.152158403245775	20.384812095997802	32.77607533351671	20.68695416723972
3	33.953698769082656	13.661291431715034	22.21378163251272	30.17122816668959
4	24.788113739513136	16.274734390042635	27.087595413285655	31.849556457158574
5	28.01927176454408	17.48158351671022	29.9124948425251	24.586649876220605
6	31.434487175079077	22.010061373951313	23.35121974281392	23.20423170815569
7	36.75764595653968	17.96445210424976	21.619919887223215	23.657982051987346
8	30.765089911979093	15.63069471186907	26.823489719433365	26.780725656718467
9	27.072445330766058	21.85748607481777	25.964125464172742	25.105943130243435
10	34.19147985146472	16.97335734424426	21.55824508320726	27.27691772108376
11	31.893287546417277	20.03764956677211	24.762971049374226	23.30609183743639
12	27.072552778159814	16.033085201485353	25.832287512034107	31.062074508320727
13	24.218642552606244	17.432050268188696	28.7071499793701	29.642157199834962
14	24.148479404483563	21.581561167652318	21.08558399807454	33.184375429789576
15	26.004203342043734	19.07395389217439	27.469033661119518	27.452809104662357
16	29.144245977169582	22.224956161463346	24.245289506257738	24.38550835510934
17	26.809951347820103	17.965419130793563	23.2535500618897	31.971079459496632
18	28.374063058726446	20.887988240957228	18.850248418374363	31.88770028194196
19	25.499415486177966	24.592881825058452	25.65693336542429	24.250769323339295
20	33.867955748865356	15.552902798789713	20.921941617384128	29.6571998349608
21	28.835012377939762	17.643506739100538	24.103458946499796	29.41802193645991
22	26.45483771145647	21.81214327465273	27.901187078806217	23.831831935084583
23	30.735971668271215	18.209109819832207	20.992427107688076	30.062491404208497
24	31.474887395131347	19.009485455920778	20.95643223077981	28.559194918168064
25	29.0704478225366	20.378115586993083	26.00647858228286	24.544958008187457
26	30.241004242388627	17.28811456408314	25.69561895557573	26.775262237952507
27	26.404528283669453	22.89541565040316	22.638126805547785	28.061929260379603
28	27.15464457431932	17.412766241942403	26.57098763712539	28.861601546612885
29	37.21057010221164	18.42529650026688	22.59251798462899	21.771615412892483
30	26.723225722613105	22.002919226984982	21.14281471030501	30.131040340096902
31	25.529586109522867	18.372793148111413	33.677289116559116	22.420331625806604
32	27.811464239052157	16.85377313126148	25.28282944381763	30.05193318586873
33	31.7814958772807	19.26262759674711	20.942494198003736	28.01338232796845
34	23.097034781670853	21.132232453016243	29.905694703045576	25.86503806226733
35	26.02906954564529	22.99038168084538	21.412085606378515	29.568463167130815
36	25.35175819171121	19.59227210623457	27.50629209712601	27.549677604928206
37	26.77077288695514	19.336861595952932	22.764276581758818	31.12808893533311
38	28.122849641491023	25.387684318603164	20.268667077036195	26.22079896286962
39	25.306015356340716	25.624511839153204	19.086345101021205	29.983127703484875
40	26.228389500489524	27.00247325925763	18.482180569899484	28.286956670353362
41	29.9601279637027	20.954424592993423	20.082469316454826	29.002978126849055
42	28.94895013740103	23.189979494967734	22.381403494017924	25.479666873613315
43	24.539084257493936	21.44992563460137	26.028014294127626	27.98297581377706
44	27.964667774525875	21.294960584486024	24.365175256355176	26.375196384632925
45	26.750555388924756	23.2603744617684	27.55644124061431	22.432628908692536
46	27.920771756978652	26.11889367816092	20.774194376026273	25.186140188834155
47	36.17694161589183	18.204344378599448	19.046693723395375	26.572020282113346
48	24.1798907157491	21.249185005742472	21.677346842459905	32.893577436048524
49	28.97117492721849	19.580203428817885	19.256011213744024	32.1926104302196
50	26.317000230043707	31.777941034603025	18.267325630579485	23.63773310477378
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	339.0
1	439.0
2	1042.5
3	1517.5
4	1369.5
5	868.0
6	377.0
7	292.5
8	333.5
9	338.5
10	314.0
11	291.5
12	278.5
13	276.0
14	275.0
15	291.5
16	318.5
17	431.5
18	553.0
19	729.5
20	926.5
21	1127.0
22	1309.0
23	1490.0
24	1662.0
25	1991.0
26	2489.0
27	2984.5
28	3424.0
29	4044.5
30	4851.0
31	5616.0
32	6308.0
33	6947.5
34	8125.5
35	9696.5
36	11485.5
37	13474.0
38	17235.0
39	23616.5
40	29101.0
41	33457.5
42	39535.0
43	60436.5
44	74963.0
45	59981.0
46	47471.0
47	42214.5
48	39418.0
49	44029.5
50	51747.5
51	58259.0
52	61625.0
53	66793.0
54	69147.0
55	64072.0
56	59646.5
57	62021.0
58	66817.5
59	67096.5
60	63098.0
61	61279.0
62	60672.5
63	59583.5
64	69353.5
65	79302.0
66	63933.0
67	45975.5
68	39766.5
69	35588.5
70	32241.5
71	31145.0
72	32065.0
73	27780.5
74	20830.0
75	18897.5
76	19056.0
77	13871.5
78	9189.0
79	9570.5
80	9979.0
81	6632.0
82	3681.0
83	3396.0
84	3035.0
85	2314.0
86	1683.5
87	939.5
88	328.0
89	234.5
90	154.5
91	77.0
92	45.5
93	38.0
94	25.5
95	10.0
96	6.0
97	6.0
98	5.5
99	5.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	6103.0
25	5763.0
26	7395.0
27	13982.0
28	18778.0
29	16795.0
30	4882.0
31	4356.0
32	4650.0
33	4235.0
34	4933.0
35	4436.0
36	3754.0
37	3729.0
38	3654.0
39	4081.0
40	4556.0
41	6024.0
42	7134.0
43	7725.0
44	6887.0
45	7316.0
46	12736.0
47	33650.0
48	176674.0
49	286946.0
50	269514.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.16979212690902
#Duplication Level	Percentage of deduplicated	Percentage of total
1	58.46111798469106	10.622263612885389
2	9.873221025698722	3.587887473199465
3	5.500641281897934	2.9983652597033945
4	4.116686409227196	2.991973452293187
5	3.360407892894606	3.052895643775967
6	2.7239383675571553	2.9696036343015244
7	2.203335280720858	2.8023900825607906
8	1.8823464305086037	2.736147468253642
9	1.554082081233347	2.5413613527747647
>10	9.64236606248652	30.166880197396672
>50	0.3834697690455244	4.8428938362792895
>100	0.23667274808273436	8.553775935536441
>500	0.03295443327734276	4.266763223438262
>1k	0.0251652035936072	9.598039888364054
>5k	0.0023966860565340187	2.9726467089197217
>10k+	0.0011983430282670093	5.296112230317441
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA	33916	3.6441858066290744	No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGC	14731	1.5828075574198872	No Hit
CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGG	9090	0.9766968092421949	No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCG	6761	0.7264518291844313	No Hit
CGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGT	5925	0.636625808004401	No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGC	5529	0.5940766400770183	No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA	4991	0.5362699422362811	No Hit
ATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGC	4770	0.5125240682162013	No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCC	4230	0.45450247558795215	No Hit
CAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGCGC	4186	0.4497747902626874	No Hit
CTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAATCCT	3484	0.3743467198459634	No Hit
ATCCGAATTCAGTCTTCCTGAGGCTCTGGCTCACCTCCAGGCAGGTCCC	3036	0.3262102874432678	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA	2841	0.30525804566084447	No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCC	2824	0.3034314399669921	No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGA	2686	0.28860369962866184	No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAAT	2685	0.28849625223490577	No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAAG	2626	0.28215685600330076	No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA	2625	0.2820494086095448	Illumina Single End Adapter 1 (100% over 32bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC	2563	0.2753876701966717	No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA	2548	0.2737759592903315	Illumina Single End Adapter 1 (100% over 32bp)
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA	2263	0.2431534520698666	No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGAC	2220	0.23853321413835785	No Hit
TCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGC	2128	0.2286480539128043	No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATC	2032	0.21833310411222664	No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCCG	2012	0.21618415623710632	No Hit
CTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAATCCTG	2007	0.21564691926832624	No Hit
AGGCCTGGTTTGCGCTGGAGAGTGGTCTTGGTTGTGAGGGTCATAAGAT	1935	0.207910706917893	No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTAG	1695	0.1821233324164489	No Hit
ATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGGGGAATCAG	1664	0.17879246321001238	No Hit
CGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTG	1657	0.17804033145372025	No Hit
CGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTC	1589	0.17073390867831112	No Hit
TACCTGGTTGATCCTGCCAGTAGCATATG	1463	0.15719553706505296	No Hit
CCCTTCCACCTTCTGCCGTGTTAGGATTTGGTGTTCCTCCCTTCCAGA	1413	0.1518231673772521	No Hit
TCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA	1376	0.14784761380827946	Illumina Single End Adapter 1 (100% over 31bp)
CGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGGGGAATCA	1326	0.1424752441204786	No Hit
CAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGG	1319	0.1417231123641865	No Hit
CTGTGCGTCTGCTCCTCCTTCTCGCGCTTCTCCTGCCGCCCTAATCCT	1308	0.1405411910328703	No Hit
TCGCTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAAT	1295	0.1391443749140421	No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTG	1291	0.138714585339018	No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG	1243	0.1335571104387292	No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA	1187	0.12754005638839225	Illumina Single End Adapter 1 (100% over 32bp)
ATCCGAATTCAGTCTTCCTGAGGCTCTGGCTCACCTCCAGGCAGGTCCCA	1160	0.12463897675697977	No Hit
AAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCGAATG	1148	0.12334960803190757	No Hit
CTGCGCGTCTGCTCCTCCTTCTCACGTTTCTCCTGCCGCCCTAATCCT	1106	0.11883681749415487	No Hit
ATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGG	1085	0.11658042222527851	No Hit
TTGTCTTTCTAGGTCTCAGCCGGTCGTCGCGACGTTCGCCCGCTCGCT	1063	0.11421657956264614	No Hit
ACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTAC	1059	0.11378678998762205	No Hit
TCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA	1023	0.10991868381240544	Illumina Single End Adapter 1 (100% over 31bp)
CGGCAAGTTTGAATTTCGTGGAGGCTCGGGTTGTGAGGGTTCCTGCTT	989	0.10626547242470086	No Hit
AACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTT	975	0.10476120891211663	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.1488146403520836	0.0	0.0	0.0	0.0
2	0.21962247283729885	0.0	0.0	0.0	0.0
3	0.26378335167102185	0.0	0.0	0.0	0.0
4	0.33674013203135744	0.0	0.0	0.0	0.0
5	0.39572875120341083	0.0	0.0	0.0	0.0
6	0.4819015609957365	0.0	0.0	0.0	0.0
7	0.542286996286618	0.0	0.0	0.0	0.0
8	0.5936468505019942	0.0	0.0	0.0	0.0
9	0.6369481501856691	0.0	0.0	0.0	0.0
10	0.674124948425251	0.0	0.0	0.0	0.0
11	0.7128060101774172	0.0	0.0	0.0	0.0
12	0.7396678586164214	0.0	0.0	0.0	0.0
13	0.7676041809929859	0.0	0.0	0.0	0.0
14	0.7951107137945262	0.0	0.0	0.0	0.0
15	0.8229395887773346	0.0	0.0	0.0	0.0
16	0.8480822789162427	0.0	0.0	0.0	0.0
17	0.8738696534176867	0.0	0.0	0.0	0.0
18	0.89428465823133	0.0	0.0	0.0	0.0
19	0.9202869275202861	0.0	0.0	0.0	0.0
20	0.9328582725897401	0.0	0.0	0.0	0.0
21	0.9502647503782148	0.0	0.0	0.0	0.0
22	0.9654148328978133	0.0	0.0	0.0	0.0
23	0.9814244945674597	0.0	0.0	0.0	0.0
24	0.9976490510246183	0.0	0.0	0.0	0.0
25	1.0111874226378765	0.0	0.0	0.0	0.0
26	1.0284864530325952	0.0	0.0	0.0	0.0
27	1.045785483427314	0.0	0.0	0.0	0.0
28	1.0572823545592078	0.0	0.0	0.0	0.0
29	1.0744739375601706	0.0	0.0	0.0	0.0
30	1.0875825195984046	0.0	0.0	0.0	0.0
31	1.0990793907302985	0.0	0.0	0.0	0.0
32	1.109716682712144	0.0	0.0	0.0	0.0
33	1.1199241851189659	0.0	0.0	0.0	0.0
34	1.1322806354009076	0.0	0.0	0.0	0.0
35	1.145926454407922	0.0	0.0	0.0	0.0
36	1.1588201416586439	0.0	0.0	0.0	0.0
37	1.172358513271902	0.0	0.0	0.0	0.0
38	1.1823511208912116	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAAGCCA	3920	0.0	129.46094	44
ATTAGTC	390	0.0	115.34611	44
CGACGGG	1535	0.0	86.54481	44
TATATTA	110	0.0	63.899128	44
AATGAAG	565	0.0	63.44674	44
TTAAGCC	3885	0.0	63.409714	43
GGTCCCA	355	0.0	61.379154	44
CTATACC	35	0.004028753	60.247746	44
AATCGCA	35	0.004028753	60.247746	44
AATCCCG	280	0.0	58.974934	43
TATTAGT	385	0.0	56.89604	43
CGGGCGC	1310	0.0	56.8751	44
ACTCTCG	25	2.8813112E-4	53.919945	43
AGGTCCC	545	0.0	53.79627	43
ACATTGG	80	7.253402E-7	52.716774	44
ACTAAGT	40	0.0068339715	52.716774	44
GGCGGGC	1530	0.0	51.453415	44
CAATGAA	965	0.0	50.637253	43
GACGGGC	655	0.0	50.43641	44
AATCCTG	635	0.0	49.886562	43
>>END_MODULE