FastQCFastQC Report
Tue 24 May 2016
ERR841157.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841157.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10049266
Sequences flagged as poor quality0
Sequence length24-50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA7310547.274700460710265No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCC697540.694120346699948No Hit
CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGG633750.630643073832457No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGC619110.6160748456653451No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTACAATAGGGAAGCAGTGGT511680.5091715156111899No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGC486910.48452294923828265No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA464550.4622725679666555No Hit
ATCCGAATTCAGTCTTCCTGAGGCTCTGGCTCACCTCCAGGCAGGTCCC412280.4102588189027935No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCCG409200.40719391844140657No Hit
CGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGT369200.3673900163454724No Hit
TACCTGGTTGATCCTGCCAGTAGCATATG338800.3371390507525624No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATC332300.3306709166619731No Hit
ATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGC324780.3231877830679375No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAAT318530.3169684233654478No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCC297130.29567333574412297No Hit
CTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAATCCT282840.2814533917203505No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAAG270170.2688455057314634No Hit
CAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGCGC269580.26825839817554836No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCG265400.26409889040652323No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA260340.2590636967913876No Hit
CTGCGCGTCTGCTCCTCCTTCTCACGTTTCTCCTGCCGCCCTAATCCT252870.2516303180749718No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA206440.2054279387171163Illumina Single End Adapter 1 (100% over 32bp)
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA203510.2025123028885891Illumina Single End Adapter 1 (100% over 32bp)
ACTACTTCTCCCCCGGACTCCTTGGTAGTCTGTTAGTGGGAGATCCTTG193550.19260113126670148No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTAG192910.19196426883316653No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG192050.19110848493810395No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGAC190830.18989446592417794No Hit
CGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTG189960.1890287310535914No Hit
AAGCAGTGGTATCAACGCAGAGTGCAG188790.1878644669172853No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA188010.1870882908264146No Hit
TCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGC179530.17864986358207655No Hit
CTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAATCCTG176460.1755949140962136No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGA171790.17094780852651328No Hit
AAGCAGTGGTATCAACGCAGAGTG165740.16492746833450322No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGT155110.15434958135250873No Hit
CTGCGCGTCTGCTCCTCCTTCTCACGTTTCTCCTGCCGCCCTAATCCTG152280.15153345527922138No Hit
TCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA150580.1498417894401442Illumina Single End Adapter 1 (100% over 31bp)
AGGCCTGGTTTGCGCTGGAGAGTGGTCTTGGTTGTGAGGGTCATAAGAT143270.1425676263321122No Hit
CGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGGGGAATCA128960.12832778035729175No Hit
ATCCGAATTCAGTCTTCCTGAGGCTCTGGCTCACCTCCAGGCAGGTCCCA126130.12551165428400443No Hit
TCGCTGCGCGTCTGCTCCTCCTTCTCACGTTTCTCCTGCCGCCCTAAT120140.11955101994513828No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCT115730.11516263973906155No Hit
TCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA111860.11131161221127991Illumina Single End Adapter 1 (100% over 31bp)
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG110550.11000803441763805No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTG109780.10924180930229133No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC103700.10319161618370934No Hit
TCGCTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAAT101640.10114171522576873No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGCCA819600.0104.2875744
ATTAGTC13650.082.8201744
CTATACG3700.068.70978544
TTAAGCC823950.060.1616643
AATGAAG59050.054.50288844
CGACGGG140600.052.51335544
CAATGAA99000.050.76212743
ATTAAGC825250.048.70367442
AGGTCCC73650.048.18017243
AATCCCG58500.048.16615343
GATTAAG840850.046.57015641
AGATTAA833700.046.17175740
TATTAGT14650.045.864743
AAGATTA837850.045.6732239
AAAGATT844500.045.06524738
CAAAGAT845050.044.75029437
AATCCTG67250.044.43155743
CTCAAAG840600.044.36491835
TAATCCT137700.044.2989442
TCAAAGA849100.044.17886436
Produced by FastQC (version 0.11.5)