##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841157.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10049266
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.50279373637836	39.0	37.0	39.0	34.0	39.0
2	38.01815107690452	39.0	38.0	39.0	35.0	39.0
3	38.34967976765666	39.0	38.0	39.0	35.0	40.0
4	38.7166399018595	39.0	39.0	41.0	35.0	41.0
5	39.42943653795213	41.0	39.0	41.0	36.0	41.0
6	39.376433661921176	41.0	39.0	41.0	36.0	41.0
7	39.296866557219204	41.0	39.0	41.0	35.0	41.0
8	39.26835134028694	40.0	39.0	41.0	35.0	41.0
9	39.31150364613694	40.0	39.0	41.0	35.0	41.0
10	39.22428832115699	40.0	39.0	41.0	35.0	41.0
11	39.325114391439136	40.0	39.0	41.0	35.0	41.0
12	39.33707665813603	40.0	39.0	41.0	35.0	41.0
13	39.3334269388431	41.0	39.0	41.0	35.0	41.0
14	39.32367786861249	40.0	39.0	41.0	35.0	41.0
15	39.32267580537723	40.0	39.0	41.0	35.0	41.0
16	39.26672375873024	40.0	39.0	41.0	35.0	41.0
17	39.18773629835253	40.0	39.0	41.0	35.0	41.0
18	39.16386559973634	40.0	39.0	41.0	35.0	41.0
19	39.032232602858755	40.0	38.0	41.0	35.0	41.0
20	39.008669886935024	40.0	38.0	41.0	35.0	41.0
21	38.726265281464336	40.0	38.0	41.0	35.0	41.0
22	38.675059949652045	40.0	38.0	41.0	35.0	41.0
23	38.5401165617469	40.0	38.0	41.0	35.0	41.0
24	38.422112321437204	40.0	38.0	41.0	34.0	41.0
25	38.425396591628655	40.0	38.0	41.0	34.0	41.0
26	38.315672454685156	40.0	37.0	41.0	34.0	41.0
27	38.26029776278868	40.0	37.0	41.0	34.0	41.0
28	38.33175567798355	40.0	37.0	41.0	34.0	41.0
29	38.42598008843217	40.0	37.0	41.0	34.0	41.0
30	38.47069247979669	40.0	37.0	41.0	35.0	41.0
31	38.4632008676509	40.0	37.0	41.0	35.0	41.0
32	38.447936160640005	40.0	37.0	41.0	35.0	41.0
33	38.39224742186983	40.0	36.0	41.0	35.0	41.0
34	38.3213755001345	40.0	36.0	41.0	35.0	41.0
35	38.23087981194716	40.0	36.0	41.0	34.0	41.0
36	38.05387930987345	40.0	35.0	41.0	34.0	41.0
37	38.04251993272763	39.0	35.0	41.0	34.0	41.0
38	37.91491236775637	39.0	35.0	41.0	34.0	41.0
39	37.75239808213141	39.0	35.0	41.0	34.0	41.0
40	37.61692236174387	39.0	35.0	41.0	34.0	41.0
41	37.46986990482131	39.0	35.0	41.0	34.0	41.0
42	37.35987086330245	38.0	35.0	41.0	33.0	41.0
43	37.261442826215024	38.0	35.0	41.0	33.0	41.0
44	37.1890725986598	37.0	35.0	41.0	33.0	41.0
45	37.107378845216346	37.0	35.0	41.0	33.0	41.0
46	36.99265921279371	37.0	35.0	41.0	33.0	41.0
47	36.85379139816032	37.0	35.0	40.0	33.0	41.0
48	36.826965947175935	37.0	35.0	40.0	33.0	41.0
49	37.15786751326378	38.0	35.0	41.0	33.0	41.0
50	37.543294942324245	39.0	35.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	297.0
15	2103.0
16	2922.0
17	91.0
18	0.0
19	2.0
20	5.0
21	23.0
22	63.0
23	156.0
24	417.0
25	1074.0
26	2610.0
27	6306.0
28	14204.0
29	28723.0
30	52280.0
31	88142.0
32	142603.0
33	234652.0
34	501589.0
35	472361.0
36	679316.0
37	1039369.0
38	1618229.0
39	2211359.0
40	2950369.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	46.30969067790623	22.817188837473303	30.87312048462047
2	25.65208245059888	26.975601999190786	26.943221524835742	20.42909402537459
3	31.498768168739886	13.421398139923852	21.150698966471783	33.92913472486448
4	20.370651946122234	16.04580871876613	25.712216195690313	37.87132313942133
5	24.88014547530138	19.033141325943607	33.47311136952689	22.613601829228124
6	34.84855510840294	20.11386702272584	20.943658969719777	24.09391889915144
7	37.15542010729938	18.35028548353681	21.902694186819215	22.591600222344596
8	29.519907225064994	15.946338767428387	31.00386635203009	23.529887655476532
9	24.843555738299695	19.171877826698985	30.820300706539168	25.16426572846216
10	33.946310108618874	18.182293114740915	23.450140537627327	24.421256239012877
11	28.288832239090894	26.859732840189523	22.659913669316744	22.191521251402836
12	25.542144073009908	15.482822327521234	30.7337670233826	28.241266576086254
13	23.197186739807663	15.578381545478049	25.955527498227234	35.26890421648706
14	23.262266119734516	20.454787444177516	19.833000738561406	36.449945697526566
15	22.752497545591886	19.919086627819386	29.691263023587993	27.637152803000735
16	32.617974287873366	19.261565969096647	23.854747202432495	24.26571254059749
17	27.31479095090129	17.70913417955102	19.08262752722438	35.893447342323306
18	25.72452555241348	18.224196672672413	18.178840126234093	37.87243764868001
19	23.78459282498841	28.06084543886091	25.24163456316113	22.91292717298955
20	37.914410863440175	15.795183449219078	20.69470546406076	25.595700223279987
21	26.676634890548222	19.11026138625448	27.42202266314774	26.79108106004956
22	26.87959498733539	25.304076934574127	25.074876115330213	22.741451962760266
23	34.471482792872635	17.516712165843753	21.732224025117855	26.27958101616576
24	29.321027028242657	19.038952695649613	19.57811645149009	32.06190382461764
25	25.489378216802656	25.33009824232747	23.71731846560158	25.46320507526829
26	28.278887343046456	18.575712865366008	29.014509208533923	24.130890583053613
27	26.790942939069488	27.29628947164249	22.39560098081051	23.517166608477513
28	26.280592843978802	16.632267047456743	31.620709102822737	25.46643100574173
29	39.64412436782987	17.493133088585893	22.195400172487016	20.667342371097224
30	25.08061802852731	19.604448429743215	20.626843974059717	34.688089567669756
31	24.25867270744046	17.996795568538147	36.35995261673462	21.384579107286772
32	25.84149160431015	18.11737950230451	29.424899574721923	26.616229318663414
33	32.861995568437145	18.414746416365592	20.76303623832978	27.960221776867485
34	22.001212434139898	20.49546074503221	34.361914518297056	23.141412302530835
35	25.717190476133432	21.214117335483987	20.840555503345275	32.228136685037306
36	24.04679786417106	18.67045895213326	31.69740352823403	25.58533965546165
37	23.575129505244252	19.425276267061616	22.452401328018013	34.54719289967612
38	28.742532184619694	28.261025446884847	19.834387676864093	23.162054691631365
39	24.98233830160862	29.391852630058825	19.68621721859958	25.939591849732974
40	25.190943965486607	30.136452640032978	18.56487519456428	26.10772819991613
41	33.48241305090865	21.12456546942251	19.302422565875293	26.090598913793556
42	26.00999199351577	28.330087213529037	22.776645587378518	22.883275205576673
43	23.37396744242383	22.01370172703917	29.514479527423077	25.097851303113927
44	24.259634283063715	21.217214116619974	28.92112068503977	25.60203091527654
45	24.747228304329013	28.228113618507656	25.541136760763134	21.483521316400196
46	25.51117456756548	29.758455770332432	20.34451198508609	24.385857677016
47	36.3761024396197	18.23521970950025	19.866286974099477	25.52239087678057
48	22.983713084038598	20.223377806895126	21.669503459313848	35.12340564975243
49	26.832904548129545	19.29849087149747	18.34815855356959	35.5204460268034
50	22.033117987061377	38.682141710673406	19.659208159517753	19.625532142747463
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2681.0
1	3431.5
2	7533.0
3	10643.0
4	9853.5
5	6682.0
6	3433.0
7	3067.5
8	3550.0
9	3859.0
10	3864.5
11	3691.0
12	3476.5
13	3475.0
14	3664.5
15	4207.5
16	4862.0
17	6098.0
18	7297.5
19	9057.0
20	10814.5
21	13246.0
22	15998.5
23	20017.0
24	23950.5
25	27555.0
26	32513.5
27	39554.0
28	46518.0
29	52814.5
30	60109.5
31	69545.0
32	79925.0
33	91972.0
34	104552.5
35	115940.0
36	135389.5
37	160154.5
38	198582.0
39	261231.0
40	317331.5
41	373794.5
42	444712.5
43	847110.0
44	1191412.5
45	843092.5
46	524617.0
47	506935.0
48	522447.0
49	605191.5
50	691448.0
51	714741.5
52	700362.5
53	703768.0
54	671481.5
55	619508.0
56	611826.5
57	650064.0
58	709293.0
59	718355.0
60	662183.5
61	607299.0
62	563162.5
63	529096.0
64	551625.0
65	582552.5
66	504592.5
67	412296.0
68	356055.0
69	311985.0
70	282873.5
71	274224.0
72	292720.0
73	266321.0
74	200387.5
75	170526.0
76	163231.5
77	122186.0
78	87901.5
79	85971.5
80	83246.5
81	56979.5
82	33484.0
83	28662.5
84	24551.5
85	20255.0
86	15643.0
87	9269.0
88	4340.5
89	2913.0
90	1964.0
91	1248.0
92	806.5
93	662.5
94	450.0
95	205.0
96	134.5
97	122.5
98	107.0
99	91.0
100	90.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	54287.0
25	40680.0
26	55547.0
27	125388.0
28	178439.0
29	220471.0
30	36136.0
31	41107.0
32	38175.0
33	36267.0
34	39818.0
35	38773.0
36	33157.0
37	32228.0
38	35212.0
39	38845.0
40	46218.0
41	51987.0
42	64288.0
43	63541.0
44	58679.0
45	68771.0
46	121193.0
47	308920.0
48	1568624.0
49	2842682.0
50	3809833.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	10.262982774881333
#Duplication Level	Percentage of deduplicated	Percentage of total
1	44.42911023405543	4.559751930354149
2	10.394350069186965	2.1335407143230483
3	6.486574480318072	1.9971480647846762
4	5.4038619296942	2.2183896760915434
5	4.480690520876625	2.2992624817665437
6	3.9029407355044774	2.4033488123919082
7	3.216189855056223	2.3105390758233075
8	2.8044608990256665	2.302570711962291
9	2.3436726027355625	2.1647764396652747
>10	15.238314347103287	27.405849672865173
>50	0.7379905953971971	5.203430869561107
>100	0.45967087002060125	9.179518136525177
>500	0.05063355543171249	3.5947130097030064
>1k	0.04137237226425027	8.355968002943408
>5k	0.005435786136667975	4.0389856129794985
>10k+	0.00473114719302583	19.832206788259825
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA	731054	7.274700460710265	No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCC	69754	0.694120346699948	No Hit
CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGG	63375	0.630643073832457	No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGC	61911	0.6160748456653451	No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTACAATAGGGAAGCAGTGGT	51168	0.5091715156111899	No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGC	48691	0.48452294923828265	No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA	46455	0.4622725679666555	No Hit
ATCCGAATTCAGTCTTCCTGAGGCTCTGGCTCACCTCCAGGCAGGTCCC	41228	0.4102588189027935	No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCCG	40920	0.40719391844140657	No Hit
CGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGT	36920	0.3673900163454724	No Hit
TACCTGGTTGATCCTGCCAGTAGCATATG	33880	0.3371390507525624	No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATC	33230	0.3306709166619731	No Hit
ATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGC	32478	0.3231877830679375	No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAAT	31853	0.3169684233654478	No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCC	29713	0.29567333574412297	No Hit
CTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAATCCT	28284	0.2814533917203505	No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAAG	27017	0.2688455057314634	No Hit
CAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGCGC	26958	0.26825839817554836	No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCG	26540	0.26409889040652323	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA	26034	0.2590636967913876	No Hit
CTGCGCGTCTGCTCCTCCTTCTCACGTTTCTCCTGCCGCCCTAATCCT	25287	0.2516303180749718	No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA	20644	0.2054279387171163	Illumina Single End Adapter 1 (100% over 32bp)
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA	20351	0.2025123028885891	Illumina Single End Adapter 1 (100% over 32bp)
ACTACTTCTCCCCCGGACTCCTTGGTAGTCTGTTAGTGGGAGATCCTTG	19355	0.19260113126670148	No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTAG	19291	0.19196426883316653	No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG	19205	0.19110848493810395	No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGAC	19083	0.18989446592417794	No Hit
CGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTG	18996	0.1890287310535914	No Hit
AAGCAGTGGTATCAACGCAGAGTGCAG	18879	0.1878644669172853	No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA	18801	0.1870882908264146	No Hit
TCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGC	17953	0.17864986358207655	No Hit
CTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAATCCTG	17646	0.1755949140962136	No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGA	17179	0.17094780852651328	No Hit
AAGCAGTGGTATCAACGCAGAGTG	16574	0.16492746833450322	No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGT	15511	0.15434958135250873	No Hit
CTGCGCGTCTGCTCCTCCTTCTCACGTTTCTCCTGCCGCCCTAATCCTG	15228	0.15153345527922138	No Hit
TCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA	15058	0.1498417894401442	Illumina Single End Adapter 1 (100% over 31bp)
AGGCCTGGTTTGCGCTGGAGAGTGGTCTTGGTTGTGAGGGTCATAAGAT	14327	0.1425676263321122	No Hit
CGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGGGGAATCA	12896	0.12832778035729175	No Hit
ATCCGAATTCAGTCTTCCTGAGGCTCTGGCTCACCTCCAGGCAGGTCCCA	12613	0.12551165428400443	No Hit
TCGCTGCGCGTCTGCTCCTCCTTCTCACGTTTCTCCTGCCGCCCTAAT	12014	0.11955101994513828	No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCT	11573	0.11516263973906155	No Hit
TCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA	11186	0.11131161221127991	Illumina Single End Adapter 1 (100% over 31bp)
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG	11055	0.11000803441763805	No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTG	10978	0.10924180930229133	No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC	10370	0.10319161618370934	No Hit
TCGCTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAAT	10164	0.10114171522576873	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.3147792087501714	0.0	0.0	0.0	0.0
2	0.4849906450879099	0.0	0.0	0.0	0.0
3	0.536944688298628	0.0	0.0	0.0	0.0
4	0.6048999001519116	0.0	0.0	0.0	0.0
5	0.6969663256998073	0.0	0.0	0.0	0.0
6	0.7895800549015222	0.0	0.0	0.0	0.0
7	0.8647198710831219	0.0	0.0	0.0	0.0
8	0.941451843348559	0.0	0.0	0.0	0.0
9	0.9975156394506822	0.0	0.0	0.0	0.0
10	1.0512111033780975	0.0	0.0	0.0	0.0
11	1.1088372026374862	0.0	0.0	0.0	0.0
12	1.1469693408453911	0.0	0.0	0.0	0.0
13	1.18755937000772	0.0	0.0	0.0	0.0
14	1.2337119944879555	0.0	0.0	0.0	0.0
15	1.2857555964783896	0.0	0.0	0.0	0.0
16	1.3201959227668967	0.0	0.0	0.0	0.0
17	1.3841707444105869	0.0	0.0	0.0	0.0
18	1.414212739517493	0.0	0.0	0.0	0.0
19	1.4820982945421088	0.0	0.0	0.0	0.0
20	1.5053537243416584	0.0	0.0	0.0	9.950975523983543E-6
21	1.5385004238120477	0.0	0.0	0.0	9.950975523983543E-6
22	1.557019189262181	0.0	0.0	0.0	9.950975523983543E-6
23	1.5817175105127081	0.0	0.0	0.0	9.950975523983543E-6
24	1.6072616646827738	0.0	0.0	0.0	9.950975523983543E-6
25	1.6280890564544714	0.0	0.0	0.0	9.950975523983543E-6
26	1.666390361246284	0.0	0.0	0.0	9.950975523983543E-6
27	1.6897751537276453	0.0	0.0	0.0	9.950975523983543E-6
28	1.7072988216253804	0.0	0.0	0.0	9.950975523983543E-6
29	1.7302756241102584	0.0	0.0	0.0	9.950975523983543E-6
30	1.7430128727809573	0.0	0.0	0.0	9.950975523983543E-6
31	1.7550734551160254	0.0	0.0	0.0	9.950975523983543E-6
32	1.7664872240420346	0.0	0.0	0.0	9.950975523983543E-6
33	1.777672120530992	0.0	0.0	0.0	9.950975523983543E-6
34	1.7906879965163625	0.0	0.0	0.0	9.950975523983543E-6
35	1.8029177454353382	0.0	0.0	0.0	9.950975523983543E-6
36	1.8162022977598562	0.0	0.0	0.0	9.950975523983543E-6
37	1.8277454293676771	0.0	0.0	0.0	9.950975523983543E-6
38	1.8374973853811811	0.0	0.0	0.0	9.950975523983543E-6
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAAGCCA	81960	0.0	104.28757	44
ATTAGTC	1365	0.0	82.82017	44
CTATACG	370	0.0	68.709785	44
TTAAGCC	82395	0.0	60.16166	43
AATGAAG	5905	0.0	54.502888	44
CGACGGG	14060	0.0	52.513355	44
CAATGAA	9900	0.0	50.762127	43
ATTAAGC	82525	0.0	48.703674	42
AGGTCCC	7365	0.0	48.180172	43
AATCCCG	5850	0.0	48.166153	43
GATTAAG	84085	0.0	46.570156	41
AGATTAA	83370	0.0	46.171757	40
TATTAGT	1465	0.0	45.8647	43
AAGATTA	83785	0.0	45.67322	39
AAAGATT	84450	0.0	45.065247	38
CAAAGAT	84505	0.0	44.750294	37
AATCCTG	6725	0.0	44.431557	43
CTCAAAG	84060	0.0	44.364918	35
TAATCCT	13770	0.0	44.29894	42
TCAAAGA	84910	0.0	44.178864	36
>>END_MODULE