FastQCFastQC Report
Tue 24 May 2016
ERR841156.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841156.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences878708
Sequences flagged as poor quality0
Sequence length24-50
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA316913.6065450638892558No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGC97731.1122010952443815No Hit
CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGG94301.0731665126526673No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGC71960.8189296102914735No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA57130.6501590972200094No Hit
CGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGT39120.4451990877515626No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCG38570.43893989812315354No Hit
CAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGG35580.4049126672341665No Hit
ATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGC33880.3855660811099933No Hit
CAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGCGC32440.36917838462834074No Hit
AAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCGAATG31490.35836705708836153No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA31350.356773808819312No Hit
AGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTCGCT30500.3471005157572254No Hit
AACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTT29610.33697200890398177No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGA28310.3221775606913787No Hit
ACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTCG25790.29349909184848666No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC22880.26038228854181367No Hit
ACGCACGGCCGGTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATG22830.25981327130286735No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCC22790.25935805751171037No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGAC21070.23978386449195863No Hit
ATCAGATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGG20520.23352467486354966No Hit
CCCTGTAATTGGAATGAGTCCACTTTAAATCCTTTAACGAGGATCCAT20260.23056578522102905No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA19780.22510321972714484Illumina Single End Adapter 1 (100% over 32bp)
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA19660.22373757835367378Illumina Single End Adapter 1 (100% over 32bp)
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA17330.19722137501877757No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAAG15540.17685055786450107No Hit
TACCTGGTTGATCCTGCCAGTAGCATATG13740.1563659372624353No Hit
CGCTGACCCCCTTCGCGGGGGGGATGCGTGCATTTATCAGATCAAAA13740.1563659372624353No Hit
AGTTATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGG13480.1534070476199147No Hit
TGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGAC13430.1528380303809684No Hit
AGGCCTGGTTTGCGCTGGAGAGTGGTCTTGGTTGTGAGGGTCATAAGAT13080.14885490970834453No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT12980.14771687523045196No Hit
ATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAAT12950.14737546488708422No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGGTTGTGAGGGTTCCTGCTT12620.14361995111003883No Hit
TTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAATTC12380.14088866836309671No Hit
CGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGGGGAATCA11730.1334914442567952No Hit
CGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTC11580.13178439253995639No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAAT11320.12882550289743577No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG11310.12871169944964653No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC10880.1238181511947086No Hit
CTCTGCAGGTACCATGGAGCTGAGCTATAGGCTCTTCATCTGCCTCCT10520.11972122707429543No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTAG9900.11266541331136168No Hit
CACGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAA9570.10890989953431629No Hit
TCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA9120.10378874438379986Illumina Single End Adapter 1 (100% over 31bp)
TCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGC9080.10333353059264283No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGCCA37050.075.3357444
ATTAGTC1600.060.79294644
TTAAGCC36900.053.82262443
CTATGCG509.443738E-750.74889444
ATAAGAT2450.047.7564243
CAATGAA8950.047.5990843
CGACGGG18600.045.7012944
ATTAAGC37300.044.6936342
ACCTTAG3500.042.97501842
GATTAAG37800.042.80005641
AGATTAA37300.042.52554340
AAGATTA38000.041.61424639
CACCTTA3300.041.32718325
CGTACAC200.001074789641.28485517
CGATCCG458.949428E-1041.2848556
CGACCTA303.916017E-641.2848515
CTCAAAG37950.040.770835
CAAAGAT38350.040.716337
GTCTCAA37850.040.5556533
AAAGATT39000.040.51793738
Produced by FastQC (version 0.11.5)