##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841156.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	878708
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.30277407284331	39.0	35.0	39.0	32.0	39.0
2	37.10948574498013	39.0	35.0	39.0	34.0	39.0
3	37.34937544667853	39.0	35.0	39.0	35.0	39.0
4	37.6066816280266	39.0	35.0	39.0	35.0	41.0
5	38.426471592383365	40.0	36.0	41.0	35.0	41.0
6	38.36772852870351	40.0	36.0	41.0	35.0	41.0
7	38.301440296435224	40.0	35.0	41.0	34.0	41.0
8	38.31953959677163	40.0	35.0	41.0	34.0	41.0
9	38.309900444743874	40.0	35.0	41.0	34.0	41.0
10	38.30228585605229	40.0	35.0	41.0	35.0	41.0
11	38.324360310819976	40.0	35.0	41.0	35.0	41.0
12	38.31172926614985	40.0	35.0	41.0	34.0	41.0
13	38.30300964598023	40.0	35.0	41.0	35.0	41.0
14	38.279364703633064	40.0	35.0	41.0	35.0	41.0
15	38.27909954159971	40.0	35.0	41.0	35.0	41.0
16	38.25355749577789	40.0	35.0	41.0	35.0	41.0
17	38.20202160444653	40.0	35.0	41.0	34.0	41.0
18	38.15806047060002	40.0	35.0	41.0	34.0	41.0
19	37.98711175953787	40.0	35.0	41.0	34.0	41.0
20	38.00643672300696	40.0	35.0	41.0	34.0	41.0
21	37.81160749646071	39.0	35.0	41.0	34.0	41.0
22	37.672332560987265	39.0	35.0	41.0	33.0	41.0
23	37.6095233001179	39.0	35.0	41.0	33.0	41.0
24	37.518285937990775	39.0	35.0	41.0	33.0	41.0
25	37.46615380218901	39.0	35.0	41.0	33.0	41.0
26	37.39255673902915	39.0	35.0	41.0	33.0	41.0
27	37.32811112483992	38.0	35.0	40.0	33.0	41.0
28	37.462113967158054	39.0	35.0	41.0	33.0	41.0
29	37.52298304028846	39.0	35.0	41.0	34.0	41.0
30	37.54067063748286	39.0	35.0	41.0	34.0	41.0
31	37.468039475329135	39.0	35.0	41.0	34.0	41.0
32	37.450313887799425	39.0	35.0	41.0	34.0	41.0
33	37.40484146967512	38.0	35.0	41.0	34.0	41.0
34	37.32638011680128	38.0	35.0	41.0	34.0	41.0
35	37.21169338698334	37.0	35.0	41.0	34.0	41.0
36	37.13074734661796	37.0	35.0	41.0	34.0	41.0
37	37.03120922247967	37.0	35.0	41.0	34.0	41.0
38	36.92103697841225	37.0	35.0	40.0	33.0	41.0
39	36.79292765375263	36.0	35.0	40.0	33.0	41.0
40	36.60110476601121	36.0	35.0	40.0	33.0	41.0
41	36.48189315121598	36.0	35.0	40.0	33.0	41.0
42	36.40902052073581	36.0	35.0	40.0	33.0	41.0
43	36.28632033959395	35.0	35.0	39.0	33.0	41.0
44	36.16425057580934	35.0	35.0	39.0	33.0	41.0
45	36.01506852498896	35.0	35.0	39.0	33.0	41.0
46	35.95030282852375	35.0	35.0	39.0	33.0	41.0
47	35.87726271411	35.0	35.0	39.0	33.0	41.0
48	35.75896195476195	35.0	35.0	38.0	33.0	41.0
49	35.922074148363144	35.0	35.0	39.0	33.0	41.0
50	36.03909811182436	36.0	35.0	39.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	5.0
14	101.0
15	333.0
16	53.0
17	0.0
18	0.0
19	0.0
20	3.0
21	7.0
22	19.0
23	48.0
24	149.0
25	293.0
26	614.0
27	1275.0
28	2485.0
29	4282.0
30	7776.0
31	12890.0
32	22070.0
33	42590.0
34	125763.0
35	46703.0
36	58625.0
37	101865.0
38	137116.0
39	174057.0
40	139586.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	40.92633730431497	27.153844052859426	31.919818642825604
2	31.906162229090896	24.540575481274782	24.42904810244131	19.124214187193015
3	29.108645875535444	16.494899329470087	21.345543684591465	33.050911110403
4	21.682743300391028	18.61960969969546	27.936925577097284	31.760721422816225
5	24.511897012431888	22.09459797794034	31.015308839796614	22.37819616983116
6	30.311206908324493	27.272199638560252	21.088689302931122	21.327904150184136
7	34.28909262235009	22.725638095931757	21.50384428046632	21.48142500125184
8	30.43969100087856	21.201240912794695	24.190743682770613	24.16832440355613
9	24.500630471100752	25.332078460649043	27.923610573705943	22.243680494544265
10	31.88021504299494	19.94701311470932	22.68990381332593	25.482868028969808
11	29.233374454312468	22.39947741456775	25.360074108805204	23.00707402231458
12	27.14337413566281	18.839250353928723	27.650823709355098	26.36655180105337
13	23.142386321736	18.79577743687323	27.65389640244541	30.407939838945357
14	20.9575877310779	23.950959818278655	24.10994323484024	30.981509215803204
15	22.889287453852702	23.72414954683467	29.000532600135653	24.386030399176974
16	29.15530528912904	28.01226345953377	20.301852264916217	22.530578986420974
17	26.154422174374194	23.645966578203453	22.525913045061614	27.67369820236074
18	27.95103720462315	22.908292629633507	21.06319733062633	28.077472835117014
19	24.846479148932296	25.43154267401685	24.90190142800566	24.82007674904519
20	33.00891763816877	18.30539838034933	19.90513344592288	28.780550535559023
21	27.577875699322185	19.803506967047074	26.122329602097626	26.496287731533112
22	24.909184848664175	24.432462205874987	25.760662245023376	24.89769070043746
23	29.99437810967921	20.702212794238815	22.490406369351366	26.813002726730613
24	28.073944928235544	21.667948852178426	22.73246630279911	27.525639916786922
25	25.114737233160916	24.457920155459664	25.627411196525017	24.7999314148544
26	26.37398071710697	20.339527635676795	28.019865832191552	25.266625815024685
27	24.831953587896272	25.672618683319843	23.53400363287416	25.961424095909724
28	24.298959693972755	20.037670274304908	26.985095167008772	28.678274864713565
29	32.157496183062	21.75076515743247	22.82717707052663	23.264561588978903
30	26.18477595842493	23.660695932694424	22.38759228762058	27.76693582126007
31	25.136574880417328	21.536047150952715	29.70517303530617	23.622204933323783
32	26.37193450865658	19.912813004723542	26.104088458624545	27.611164027995333
33	29.850147896448043	22.033515046324588	22.64355733737502	25.472779719852344
34	22.059916458384944	23.640486533022976	29.446825001655625	24.852772006936455
35	24.428367505040466	25.560363036669465	22.77760740163241	27.233662056657664
36	25.06624186189066	23.742640479709724	27.798718664880447	23.392398993519173
37	24.952865527831666	22.919261129937336	23.2571408725335	28.870732469697497
38	25.174215160701042	27.102776989661574	22.492794958711006	25.230212890926378
39	25.031457061111585	29.082677145696124	20.284311914237065	25.601553878955226
40	24.809479880072598	30.57923130145297	20.474520440803392	24.136768377671043
41	27.786826027745544	24.31687111236416	21.644015274900376	26.25228758498992
42	28.211457199969637	26.18177677690342	21.57523848080767	24.03152754231927
43	23.746868524516255	22.202368140524445	27.770822481160202	26.2799408537991
44	25.97066834920977	22.6531601545067	26.071723529441936	25.304447966841593
45	25.837678285054654	25.978937636707805	25.841164576183207	22.34221950205433
46	27.85112047204055	28.126702882105608	22.07364383932206	21.948532806531784
47	33.857097846277156	21.928370951305588	20.307245299236566	23.907285903180686
48	24.25989322868379	24.676683281073135	21.113349735562487	29.950073754680584
49	26.432954975658028	23.278959638158113	21.94397029814762	28.344115088036236
50	27.540437410126035	29.88726397804491	21.507955901841182	21.064342709987876
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	567.0
1	670.0
2	975.0
3	1164.5
4	1088.5
5	807.0
6	552.5
7	526.0
8	555.0
9	580.0
10	585.5
11	580.0
12	586.0
13	657.0
14	740.5
15	839.0
16	910.5
17	1050.5
18	1208.5
19	1523.5
20	1859.5
21	2305.0
22	2767.5
23	3507.5
24	4302.5
25	4880.5
26	5457.5
27	6421.5
28	7580.0
29	8653.0
30	9833.5
31	11362.0
32	12968.5
33	14528.0
34	15948.0
35	17378.5
36	19708.0
37	22354.0
38	25941.5
39	32455.0
40	37882.0
41	42904.0
42	50483.0
43	69447.5
44	81320.5
45	64772.0
46	51970.5
47	50205.5
48	48547.5
49	49714.0
50	54564.5
51	59520.5
52	59942.5
53	60183.0
54	60183.0
55	54731.5
56	49226.5
57	49096.0
58	50815.0
59	51520.0
60	49459.0
61	44478.0
62	40869.5
63	39510.0
64	43725.5
65	47753.0
66	39365.5
67	30472.5
68	27092.5
69	25173.5
70	22649.0
71	21844.0
72	23608.5
73	20222.0
74	14167.0
75	12081.0
76	11672.0
77	8366.0
78	5381.5
79	6070.5
80	6843.0
81	4631.5
82	2429.0
83	2348.0
84	2356.0
85	1967.5
86	1525.5
87	892.0
88	352.5
89	245.0
90	187.5
91	123.0
92	60.0
93	33.5
94	20.0
95	6.0
96	5.0
97	4.0
98	5.0
99	6.0
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	3883.0
25	3379.0
26	5465.0
27	8541.0
28	9902.0
29	20504.0
30	3127.0
31	2913.0
32	2854.0
33	2738.0
34	3473.0
35	2773.0
36	2952.0
37	2600.0
38	2512.0
39	3276.0
40	3310.0
41	4086.0
42	5247.0
43	5401.0
44	5310.0
45	5545.0
46	10370.0
47	35881.0
48	120414.0
49	176656.0
50	425596.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.734240622113134
#Duplication Level	Percentage of deduplicated	Percentage of total
1	55.63320239116267	10.422458001747612
2	9.566322918376297	3.5843559084339414
3	5.775022857047548	3.2457200340639605
4	4.655121044606704	3.4884063109889856
5	3.7398164482981047	3.503131061247661
6	3.1026783798595194	3.487579400478982
7	2.5402046002978778	3.3312162947965405
8	2.123081839175223	3.1819460828437696
9	1.7286384875477894	2.91462564369294
>10	10.58706685883334	33.36331852828892
>50	0.3208818087401789	4.120879099771179
>100	0.1796442558583787	6.371701430449041
>500	0.02292012919572418	2.9339025182715686
>1k	0.02230066624448839	8.646314355496418
>5k	0.0024778518049431548	3.7266515433225194
>10k+	6.194629512357887E-4	3.67779378610594
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA	31691	3.6065450638892558	No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGC	9773	1.1122010952443815	No Hit
CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGG	9430	1.0731665126526673	No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGC	7196	0.8189296102914735	No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA	5713	0.6501590972200094	No Hit
CGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGT	3912	0.4451990877515626	No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCG	3857	0.43893989812315354	No Hit
CAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGG	3558	0.4049126672341665	No Hit
ATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGC	3388	0.3855660811099933	No Hit
CAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGCGC	3244	0.36917838462834074	No Hit
AAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCGAATG	3149	0.35836705708836153	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA	3135	0.356773808819312	No Hit
AGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTCGCT	3050	0.3471005157572254	No Hit
AACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTT	2961	0.33697200890398177	No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGA	2831	0.3221775606913787	No Hit
ACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTCG	2579	0.29349909184848666	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC	2288	0.26038228854181367	No Hit
ACGCACGGCCGGTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATG	2283	0.25981327130286735	No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCC	2279	0.25935805751171037	No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGAC	2107	0.23978386449195863	No Hit
ATCAGATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGG	2052	0.23352467486354966	No Hit
CCCTGTAATTGGAATGAGTCCACTTTAAATCCTTTAACGAGGATCCAT	2026	0.23056578522102905	No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA	1978	0.22510321972714484	Illumina Single End Adapter 1 (100% over 32bp)
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA	1966	0.22373757835367378	Illumina Single End Adapter 1 (100% over 32bp)
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA	1733	0.19722137501877757	No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAAG	1554	0.17685055786450107	No Hit
TACCTGGTTGATCCTGCCAGTAGCATATG	1374	0.1563659372624353	No Hit
CGCTGACCCCCTTCGCGGGGGGGATGCGTGCATTTATCAGATCAAAA	1374	0.1563659372624353	No Hit
AGTTATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGG	1348	0.1534070476199147	No Hit
TGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGAC	1343	0.1528380303809684	No Hit
AGGCCTGGTTTGCGCTGGAGAGTGGTCTTGGTTGTGAGGGTCATAAGAT	1308	0.14885490970834453	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT	1298	0.14771687523045196	No Hit
ATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAAT	1295	0.14737546488708422	No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGGTTGTGAGGGTTCCTGCTT	1262	0.14361995111003883	No Hit
TTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAATTC	1238	0.14088866836309671	No Hit
CGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGGGGAATCA	1173	0.1334914442567952	No Hit
CGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTC	1158	0.13178439253995639	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAAT	1132	0.12882550289743577	No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG	1131	0.12871169944964653	No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC	1088	0.1238181511947086	No Hit
CTCTGCAGGTACCATGGAGCTGAGCTATAGGCTCTTCATCTGCCTCCT	1052	0.11972122707429543	No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTAG	990	0.11266541331136168	No Hit
CACGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAA	957	0.10890989953431629	No Hit
TCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA	912	0.10378874438379986	Illumina Single End Adapter 1 (100% over 31bp)
TCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGC	908	0.10333353059264283	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.32798153652863066	0.0	0.0	0.0	0.0
2	0.43882609467536426	0.0	0.0	0.0	0.0
3	0.5168952598587927	0.0	0.0	0.0	0.0
4	0.5998579732971591	0.0	0.0	0.0	0.0
5	0.6860071832736244	0.0	0.0	0.0	0.0
6	0.7938928517778374	0.0	0.0	0.0	0.0
7	0.8737828721258939	0.0	0.0	0.0	0.0
8	0.9480965235322769	0.0	0.0	0.0	0.0
9	1.0006737164109123	0.0	0.0	0.0	0.0
10	1.0540475334240726	0.0	0.0	0.0	0.0
11	1.1065109228549188	0.0	0.0	0.0	0.0
12	1.1454317019988438	0.0	0.0	0.0	0.0
13	1.1865147466507646	0.0	0.0	0.0	0.0
14	1.2285082188849994	4.552137911570169E-4	0.0	0.0	0.0
15	1.2708431014626018	4.552137911570169E-4	0.0	0.0	0.0
16	1.3078292219941094	4.552137911570169E-4	0.0	0.0	0.0
17	1.3475466252725592	4.552137911570169E-4	0.0	0.0	0.0
18	1.3753146665331373	4.552137911570169E-4	0.0	0.0	0.0
19	1.4097971112132812	4.552137911570169E-4	0.0	0.0	0.0
20	1.4327854076667106	4.552137911570169E-4	0.0	0.0	0.0
21	1.4572531489414002	4.552137911570169E-4	0.0	0.0	0.0
22	1.4743236661097885	4.552137911570169E-4	0.0	0.0	0.0
23	1.4970843556676392	4.552137911570169E-4	0.0	0.0	0.0
24	1.5204140624644364	4.552137911570169E-4	0.0	0.0	0.0
25	1.5391916313496634	4.552137911570169E-4	0.0	0.0	0.0
26	1.5620661243553036	4.552137911570169E-4	0.0	0.0	0.0
27	1.5792504449714808	4.552137911570169E-4	0.0	0.0	0.0
28	1.596776175931026	4.552137911570169E-4	0.0	0.0	0.0
29	1.6187402413543521	4.552137911570169E-4	0.0	0.0	0.0
30	1.6351279378360046	4.552137911570169E-4	0.0	0.0	0.0
31	1.6494671722574508	4.552137911570169E-4	0.0	0.0	0.0
32	1.6628959790965827	4.552137911570169E-4	0.0	0.0	0.0
33	1.6738211100843512	4.552137911570169E-4	0.0	0.0	0.0
34	1.6923710720739995	4.552137911570169E-4	0.0	0.0	0.0
35	1.7063688961520778	4.552137911570169E-4	0.0	0.0	0.0
36	1.7201391133345776	4.552137911570169E-4	0.0	0.0	0.0
37	1.733340313278131	4.552137911570169E-4	0.0	0.0	0.0
38	1.7434688201313746	4.552137911570169E-4	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAAGCCA	3705	0.0	75.33574	44
ATTAGTC	160	0.0	60.792946	44
TTAAGCC	3690	0.0	53.822624	43
CTATGCG	50	9.443738E-7	50.748894	44
ATAAGAT	245	0.0	47.75642	43
CAATGAA	895	0.0	47.59908	43
CGACGGG	1860	0.0	45.70129	44
ATTAAGC	3730	0.0	44.69363	42
ACCTTAG	350	0.0	42.975018	42
GATTAAG	3780	0.0	42.800056	41
AGATTAA	3730	0.0	42.525543	40
AAGATTA	3800	0.0	41.614246	39
CACCTTA	330	0.0	41.327183	25
CGTACAC	20	0.0010747896	41.284855	17
CGATCCG	45	8.949428E-10	41.284855	6
CGACCTA	30	3.916017E-6	41.28485	15
CTCAAAG	3795	0.0	40.7708	35
CAAAGAT	3835	0.0	40.7163	37
GTCTCAA	3785	0.0	40.55565	33
AAAGATT	3900	0.0	40.517937	38
>>END_MODULE