##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841155.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3807127
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.19452962824723	39.0	37.0	39.0	33.0	39.0
2	37.851680545461186	39.0	37.0	39.0	35.0	39.0
3	38.17898299688978	39.0	38.0	39.0	35.0	40.0
4	38.545887226772315	39.0	38.0	41.0	35.0	41.0
5	39.08826366969108	40.0	38.0	41.0	35.0	41.0
6	39.05040362457044	40.0	38.0	41.0	35.0	41.0
7	38.97570215020408	40.0	38.0	41.0	35.0	41.0
8	38.94746799883482	40.0	38.0	41.0	35.0	41.0
9	38.92150905394015	40.0	38.0	41.0	35.0	41.0
10	38.874227731305	40.0	38.0	41.0	35.0	41.0
11	38.825806178779956	40.0	38.0	41.0	35.0	41.0
12	38.75827572865313	40.0	38.0	41.0	35.0	41.0
13	38.68309147554048	40.0	38.0	41.0	35.0	41.0
14	38.618267790908995	40.0	37.0	41.0	35.0	41.0
15	38.5522584352978	40.0	37.0	41.0	35.0	41.0
16	38.453840389353964	40.0	37.0	41.0	35.0	41.0
17	38.34074093141626	40.0	37.0	41.0	35.0	41.0
18	38.23015307868637	40.0	36.0	41.0	34.0	41.0
19	38.04822166426284	39.0	36.0	41.0	34.0	41.0
20	37.95437320583211	39.0	35.0	41.0	34.0	41.0
21	37.59896005570605	39.0	35.0	41.0	33.0	41.0
22	37.44981478159252	39.0	35.0	40.0	33.0	41.0
23	37.30745204980028	38.0	35.0	40.0	33.0	41.0
24	37.1465785092013	38.0	35.0	40.0	33.0	41.0
25	36.99790246958858	38.0	35.0	40.0	33.0	41.0
26	36.79132350853733	37.0	35.0	40.0	33.0	41.0
27	36.72277364172348	37.0	35.0	40.0	33.0	41.0
28	36.76849399314702	37.0	35.0	40.0	33.0	41.0
29	36.77520202258807	37.0	35.0	40.0	33.0	41.0
30	36.73255501931644	37.0	35.0	40.0	33.0	41.0
31	36.60262227861328	36.0	35.0	40.0	33.0	41.0
32	36.525163711415445	36.0	35.0	40.0	33.0	41.0
33	36.452520962834846	36.0	35.0	40.0	33.0	41.0
34	36.347993405332666	35.0	35.0	40.0	33.0	41.0
35	36.23317102907965	35.0	35.0	39.0	33.0	41.0
36	36.09739021409153	35.0	35.0	39.0	33.0	41.0
37	36.00869694844016	35.0	35.0	39.0	33.0	41.0
38	35.86698333758271	35.0	35.0	39.0	33.0	41.0
39	35.72101672138139	35.0	35.0	38.0	33.0	41.0
40	35.5766947679868	35.0	35.0	37.0	33.0	41.0
41	35.449888402895134	35.0	35.0	37.0	33.0	41.0
42	35.36319285364558	35.0	35.0	37.0	33.0	40.0
43	35.28812064940105	35.0	35.0	37.0	33.0	40.0
44	35.22695129690995	35.0	35.0	36.0	33.0	40.0
45	35.11897983146759	35.0	35.0	36.0	33.0	39.0
46	35.01453608631101	35.0	35.0	36.0	33.0	39.0
47	34.964816722590406	35.0	35.0	36.0	33.0	39.0
48	34.86858398557541	35.0	35.0	36.0	33.0	39.0
49	34.972830612124646	35.0	35.0	36.0	32.0	39.0
50	35.39039664909543	35.0	35.0	37.0	32.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	37.0
14	387.0
15	671.0
16	61.0
17	0.0
18	0.0
19	1.0
20	7.0
21	12.0
22	41.0
23	103.0
24	323.0
25	765.0
26	1996.0
27	4455.0
28	9178.0
29	17762.0
30	31504.0
31	52156.0
32	84912.0
33	146598.0
34	312701.0
35	375551.0
36	554382.0
37	713302.0
38	591550.0
39	557117.0
40	351554.0
41	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	43.5916637401379	19.85948984628041	36.548846413581686
2	28.37357408880765	20.39467031176002	28.892469308221138	22.339286291211195
3	32.483681264113336	13.268850763318376	22.47658142215902	31.770886550409273
4	21.726777173443388	16.156041025161493	28.24523584319619	33.87194595819892
5	27.16907001001017	18.30695429913423	30.971412301191947	23.55256338966365
6	31.20809996619498	21.311030601290685	21.692210425341734	25.788659007172598
7	34.49170463711875	18.514328521218232	21.90021504404765	25.093751797615365
8	30.35543600200361	16.18243888370417	27.767421470310815	25.69470364398141
9	25.39684124012674	20.18929234564542	26.81074206350353	27.60312435072431
10	32.41882921163386	17.407982449758048	22.442513738049715	27.730674600558373
11	31.23300063276061	20.135787432360413	24.81750674458719	23.81370519029179
12	27.392230414167955	15.273144289644133	26.16014648316171	31.1744788130262
13	24.9341301196414	15.919537225839854	28.49839261994675	30.647940034572002
14	24.704481883583078	19.953707874730735	20.845403896428987	34.4964063452572
15	25.397970700741006	19.575837632944737	26.373036675687466	28.65315499062679
16	29.361116663562836	20.868282040499306	25.211058102343316	24.55954319359454
17	27.854626336342335	18.280451374487903	20.318996450604352	33.5459258385654
18	28.397030096448056	19.12812469875578	18.712325593551252	33.76251961124491
19	26.933538072147318	21.85435368980336	26.105958640202964	25.106149597846354
20	34.72355926135377	16.003117311295366	21.350377857108523	27.922945570242337
21	28.76386839735055	18.32901818090124	24.661772512448362	28.24534090929985
22	28.44817102240088	21.209510478636513	25.865751260727578	24.476567238235024
23	32.03300021249619	17.904078324678952	21.422269338532704	28.64065212429215
24	31.30066320351278	18.88610492899239	20.47328602381796	29.339945843676873
25	28.355579988017883	20.559256950379833	25.689202708729226	25.395960352873054
26	30.0327272100153	17.693653680584024	25.808064929086623	26.46555418031406
27	27.98421548138108	22.375022535004373	23.380637924242322	26.260124059372224
28	28.59420710975901	16.963581380448282	26.450544709980424	27.991666799812275
29	36.9975474655348	17.865927563343327	23.30452012849214	21.83200484262973
30	26.996770351044635	20.487835402341876	21.462874821647723	31.05251942496577
31	26.825345296967708	17.976669158225697	32.704217036773095	22.49376850803351
32	28.07571551715073	17.3926734931743	25.099338105958186	29.43227288371679
33	30.551455268820888	19.56364767596509	21.84835408211609	28.036542973097934
34	23.831190964698003	20.423544831496844	29.967522610758962	25.777741593046187
35	26.517111666114	21.377460960694062	22.038182649472652	30.067244723719288
36	25.810342996216328	19.149746563858585	27.821219057795826	27.218691382129258
37	25.424357267352487	19.13686077617846	23.346696414924264	32.09208554154479
38	29.00914072942636	24.17109447793057	20.445344365764534	26.374420426878544
39	26.090720992410237	25.804464580711826	19.867038539316102	28.237775887561835
40	27.080208389431505	25.704835787232778	18.99612749741691	28.21882832591881
41	29.980420211028587	20.91880630150863	20.42499207104345	28.675781416419333
42	28.391685830753865	23.06663738288848	23.021847506413774	25.519829279943878
43	24.26704461478011	22.0330312071549	26.2197298047495	27.480194373315488
44	26.55797014820574	21.81471452485274	24.734732938210836	26.892582388730684
45	26.14605572957755	23.086147592054516	27.466167320417632	23.301629357950297
46	26.482067187722514	25.380326579046198	22.445734968945494	25.691871264285798
47	34.550664589365134	18.396358273786216	19.584170311178895	27.468806825669763
48	23.775562992221737	21.818343761975846	22.039997607295383	32.36609563850703
49	28.315813924492755	20.35705776409132	19.119640098481025	32.2074882129349
50	26.03745706738239	31.144553509249047	19.29862463124969	23.519364792118868
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1191.0
1	1315.0
2	2723.5
3	3932.0
4	3657.0
5	2512.5
6	1328.0
7	1125.0
8	1179.0
9	1239.0
10	1259.5
11	1216.5
12	1119.5
13	1030.5
14	1021.5
15	1158.0
16	1345.5
17	1675.0
18	2004.0
19	2536.5
20	3081.0
21	3642.0
22	4268.0
23	5393.0
24	6478.5
25	7375.5
26	8925.5
27	11165.5
28	13202.0
29	15186.5
30	17576.5
31	20889.0
32	24838.5
33	28721.0
34	32805.0
35	37190.5
36	43853.5
37	51254.5
38	63878.0
39	87584.5
40	111247.5
41	132510.0
42	160852.0
43	246342.5
44	302281.0
45	234223.0
46	179562.5
47	168336.5
48	165361.0
49	188387.5
50	223150.0
51	246325.0
52	255481.5
53	270150.0
54	273522.5
55	259064.0
56	254824.0
57	274829.0
58	307722.0
59	310197.5
60	275762.5
61	252821.5
62	244201.0
63	237029.0
64	259542.5
65	283827.0
66	238934.5
67	184608.5
68	161549.0
69	144672.5
70	131022.5
71	126349.0
72	131328.5
73	117761.5
74	89990.5
75	76782.5
76	72825.0
77	54644.5
78	38860.5
79	36785.5
80	34997.0
81	24507.0
82	15123.0
83	13162.0
84	11197.0
85	8667.0
86	6425.0
87	3760.5
88	1607.5
89	1087.0
90	747.0
91	439.5
92	281.0
93	248.5
94	176.0
95	84.5
96	56.5
97	51.0
98	43.0
99	34.5
100	34.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	19816.0
25	18276.0
26	24860.0
27	51476.0
28	73178.0
29	71767.0
30	14491.0
31	14724.0
32	14719.0
33	15547.0
34	17220.0
35	15942.0
36	13789.0
37	13368.0
38	13882.0
39	16011.0
40	18045.0
41	24347.0
42	26817.0
43	24729.0
44	23567.0
45	29337.0
46	56690.0
47	151947.0
48	700608.0
49	1161636.0
50	1180338.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	11.796845553987119
#Duplication Level	Percentage of deduplicated	Percentage of total
1	45.62915924829454	5.382801444104138
2	10.27061146034493	2.4232163428539852
3	6.470539153387413	2.2899585313061364
4	5.323118629410975	2.511840333468515
5	4.5213453361316684	2.666880631329264
6	3.7566115126436435	2.6589699492592196
7	3.168429776417705	2.6164233704737523
8	2.629386700856527	2.4814775049369744
9	2.229390805007468	2.36698011055369
>10	14.808238700642972	30.735346228262756
>50	0.6789429175434519	5.501928584501995
>100	0.42545104951367696	9.994359212315159
>500	0.04380176974554587	3.605121865126757
>1k	0.03607204567280248	8.83115037869095
>5k	0.0037477450049664914	2.8961064595308446
>10k+	0.005153149381828925	13.037439053285912
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA	126422	3.320666738987168	No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGC	36054	0.947013325271261	No Hit
CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGG	27801	0.7302356869103658	No Hit
ATCCGAATTCAGTCTTCCTGAGGCTCTGGCTCACCTCCAGGCAGGTCCC	24348	0.6395373729323975	No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA	22939	0.6025278379208259	No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGC	20618	0.541563231276498	No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCC	18786	0.4934429558036808	No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCG	16505	0.4335290101958773	No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAAG	16309	0.4283807711169078	No Hit
CGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGT	15841	0.4160880369895725	No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGAC	15649	0.4110448640142554	No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCCG	14672	0.38538246819714705	No Hit
CTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAATCCT	12999	0.3414385703445144	No Hit
ATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGC	12898	0.33878565122729026	No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATC	12452	0.32707078067004336	No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGA	12387	0.32536345648569115	No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCC	11315	0.2972057407068375	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA	11097	0.29147963805777954	No Hit
CAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGCGC	10823	0.2842826099575875	No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC	10727	0.2817610234699289	No Hit
ATCCGAATTCAGTCTTCCTGAGGCTCTGGCTCACCTCCAGGCAGGTCCCA	10630	0.27921317045635724	No Hit
CTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAATCCTG	10548	0.2770593153314822	No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAAT	9227	0.24236123460026418	No Hit
TCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA	8393	0.2204549519887306	Illumina Single End Adapter 1 (100% over 31bp)
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTAG	8027	0.21084140350453243	No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA	7786	0.20451117075947295	Illumina Single End Adapter 1 (100% over 32bp)
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA	7322	0.19232350273579002	Illumina Single End Adapter 1 (100% over 32bp)
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA	7221	0.18967058361856592	No Hit
AGGCCTGGTTTGCGCTGGAGAGTGGTCTTGGTTGTGAGGGTCATAAGAT	6446	0.1693140260359058	No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG	5975	0.1569424923308311	No Hit
TACCTGGTTGATCCTGCCAGTAGCATATG	5930	0.15576049866474115	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAAT	5901	0.15499876941326096	No Hit
TCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGC	5803	0.1524246498737762	No Hit
TCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA	5678	0.14914133413463748	Illumina Single End Adapter 1 (100% over 31bp)
CGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTG	5512	0.14478109083306126	No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTG	5381	0.14134017593844386	No Hit
CCCTTCCACCTTCTGCCGTGTTAGGATTTGGTGTTCCTCCCTTCCAGA	5175	0.13592927160034327	No Hit
ACTACTTCTCCCCCGGACTCCTTGGTAGTCTGTTAGTGGGAGATCCTTG	5032	0.13217315839476854	No Hit
CTGTGCGTCTGCTCCTCCTTCTCGCGCTTCTCCTGCCGCCCTAATCCT	4809	0.12631572311614506	No Hit
CCCTTCCACCTTCTGCCGTGTTAGGATTTGGTGTTCCTCCCTTCCAGAA	4799	0.12605305785701396	No Hit
TCGCTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAAT	4647	0.12206054591822127	No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGT	4632	0.12166654802952463	No Hit
AGGCCTGGTTTGCGCTGGAGAGTGGTCTTGGTTGTGAGGGTCATAAGATG	4618	0.1212988166667411	No Hit
ATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGGGGAATCAG	4585	0.12043202131160848	No Hit
CTGCGCGTCTGCTCCTCCTTCTCACGTTTCTCCTGCCGCCCTAATCCT	4355	0.11439072035159321	No Hit
CGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTC	4090	0.1074300909846191	No Hit
TCGCTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAATC	3983	0.10461957271191637	No Hit
CGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGGGGAATCA	3868	0.10159892223190874	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.09676588146389653	0.0	0.0	0.0	0.0
2	0.1615391343656253	0.0	0.0	0.0	0.0
3	0.21588457647984952	0.0	0.0	0.0	0.0
4	0.2897985803993405	0.0	0.0	0.0	0.0
5	0.3709358789449367	0.0	0.0	0.0	0.0
6	0.4807562237876488	0.0	0.0	0.0	0.0
7	0.5558259548473167	0.0	0.0	0.0	0.0
8	0.6281114341601948	0.0	0.0	0.0	0.0
9	0.6849521962361644	0.0	0.0	0.0	0.0
10	0.7381419112102118	0.0	0.0	0.0	0.0
11	0.7921196219616524	0.0	0.0	0.0	0.0
12	0.834356195629933	0.0	0.0	0.0	0.0
13	0.8739923832328157	0.0	0.0	0.0	0.0
14	0.9150469632350063	0.0	0.0	0.0	0.0
15	0.9568107394368509	0.0	0.0	0.0	0.0
16	0.9894074980950202	0.0	0.0	0.0	0.0
17	1.0335352616290447	0.0	0.0	0.0	0.0
18	1.061587911304246	0.0	0.0	0.0	0.0
19	1.1025899582546104	0.0	0.0	0.0	0.0
20	1.1253893027471897	0.0	0.0	0.0	0.0
21	1.1518396943416913	0.0	0.0	0.0	0.0
22	1.171381989621045	0.0	0.0	0.0	0.0
23	1.1906090865894414	0.0	0.0	0.0	0.0
24	1.2102827144983606	0.0	0.0	0.0	0.0
25	1.228800615267103	0.0	0.0	0.0	0.0
26	1.2557763373798667	0.0	0.0	0.0	0.0
27	1.2742679716226961	0.0	0.0	0.0	0.0
28	1.290290552429693	0.0	0.0	0.0	0.0
29	1.3092549841389582	0.0	0.0	0.0	0.0
30	1.324962366634998	0.0	0.0	0.0	0.0
31	1.3386209601098151	0.0	0.0	0.0	0.0
32	1.3502570310893227	0.0	0.0	0.0	0.0
33	1.3638630915123136	0.0	0.0	0.0	0.0
34	1.3784672799200026	0.0	0.0	0.0	0.0
35	1.392178406446646	0.0	0.0	0.0	0.0
36	1.4064936630692908	0.0	0.0	0.0	0.0
37	1.4193642607667147	0.0	0.0	0.0	0.0
38	1.4301335363910896	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAAGCCA	14215	0.0	122.78906	44
ATTAGTC	1435	0.0	114.302155	44
CTATACG	75	1.8189894E-12	78.41766	44
CGACGGG	5195	0.0	77.48681	44
AATGAAG	3015	0.0	76.076836	44
TTAAGCC	14090	0.0	63.21221	43
CCAGAAT	1570	0.0	60.3533	44
GGTCCCA	2280	0.0	59.90238	44
TATTAGT	1415	0.0	59.746784	43
CGCTACC	515	0.0	58.369133	44
CAATGAA	5175	0.0	54.43393	43
AGGTCCC	4485	0.0	54.119762	43
CTATCAT	510	0.0	53.816044	44
AATCCCG	2175	0.0	52.838566	43
AATCCTG	3135	0.0	51.19526	43
TTTGGAC	2475	0.0	49.636223	43
ATTAAGC	14035	0.0	49.053345	42
AATCGCA	135	9.094947E-12	48.405964	44
ACTACAC	370	0.0	45.92025	44
ATGTTCT	570	0.0	45.858284	44
>>END_MODULE