##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR841153.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 879405 Sequences flagged as poor quality 0 Sequence length 24-50 %GC 53 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.382266418771785 39.0 37.0 39.0 34.0 39.0 2 37.9563977916887 39.0 38.0 39.0 35.0 39.0 3 38.297377203904915 39.0 38.0 39.0 35.0 40.0 4 38.59318289070451 39.0 38.0 40.0 35.0 41.0 5 39.30318795094411 40.0 39.0 41.0 35.0 41.0 6 39.27906027370779 40.0 39.0 41.0 35.0 41.0 7 39.23081060489763 40.0 39.0 41.0 35.0 41.0 8 39.23812691535754 40.0 39.0 41.0 35.0 41.0 9 39.221263240486465 40.0 39.0 41.0 35.0 41.0 10 39.1980691490269 40.0 39.0 41.0 35.0 41.0 11 39.229574541877746 40.0 39.0 41.0 35.0 41.0 12 39.19224703066278 40.0 39.0 41.0 35.0 41.0 13 39.17231196092813 40.0 39.0 41.0 35.0 41.0 14 39.14968757284755 40.0 38.0 41.0 35.0 41.0 15 39.14696300339434 40.0 38.0 41.0 35.0 41.0 16 39.12158334328324 40.0 38.0 41.0 35.0 41.0 17 39.06530893047003 40.0 38.0 41.0 35.0 41.0 18 39.00485100721511 40.0 38.0 41.0 35.0 41.0 19 38.8941500218898 40.0 38.0 41.0 35.0 41.0 20 38.846141425168156 40.0 38.0 41.0 35.0 41.0 21 38.61736401316799 40.0 38.0 41.0 35.0 41.0 22 38.538856385851794 40.0 38.0 41.0 34.0 41.0 23 38.41754936576435 40.0 38.0 41.0 34.0 41.0 24 38.35448058630551 40.0 37.0 41.0 34.0 41.0 25 38.2944250811374 40.0 37.0 41.0 34.0 41.0 26 38.20149649159428 40.0 37.0 41.0 34.0 41.0 27 38.181757064524874 40.0 37.0 41.0 34.0 41.0 28 38.29145929052872 40.0 37.0 41.0 34.0 41.0 29 38.37272058745164 40.0 37.0 41.0 34.0 41.0 30 38.422433518506644 40.0 37.0 41.0 35.0 41.0 31 38.37174938887782 40.0 37.0 41.0 35.0 41.0 32 38.32107831172382 40.0 36.0 41.0 34.0 41.0 33 38.27927098854992 40.0 36.0 41.0 35.0 41.0 34 38.21291014463489 40.0 36.0 41.0 35.0 41.0 35 38.1142317374124 40.0 35.0 41.0 34.0 41.0 36 38.04641306761718 39.0 35.0 41.0 34.0 41.0 37 37.956971923052976 39.0 35.0 41.0 34.0 41.0 38 37.836638665958844 39.0 35.0 41.0 34.0 41.0 39 37.6935631247454 39.0 35.0 41.0 34.0 41.0 40 37.503470666136266 39.0 35.0 41.0 34.0 41.0 41 37.32648720193958 38.0 35.0 41.0 33.0 41.0 42 37.246537177332954 38.0 35.0 41.0 33.0 41.0 43 37.15722857594299 37.0 35.0 41.0 33.0 41.0 44 37.07657356190752 37.0 35.0 41.0 33.0 41.0 45 36.967007239896866 37.0 35.0 41.0 33.0 41.0 46 36.872590860116155 37.0 35.0 40.0 33.0 41.0 47 36.792737241523824 37.0 35.0 40.0 33.0 41.0 48 36.74684815554593 37.0 35.0 40.0 33.0 41.0 49 37.020794967315666 37.0 35.0 40.0 33.0 41.0 50 37.2584622182943 38.0 35.0 40.0 33.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 14 19.0 15 128.0 16 141.0 17 4.0 18 0.0 19 0.0 20 0.0 21 5.0 22 7.0 23 24.0 24 49.0 25 107.0 26 280.0 27 519.0 28 1126.0 29 2198.0 30 4296.0 31 7464.0 32 12510.0 33 22007.0 34 49352.0 35 42949.0 36 63288.0 37 93901.0 38 151898.0 39 194856.0 40 232277.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 42.76516508320967 22.526026119933366 34.708808796856964 2 29.539063343965523 23.429023032618645 25.931851649694966 21.10006197372087 3 30.24874773284209 15.32024493833899 21.528192357332514 32.90281497148641 4 22.890135944189538 17.515365502811562 29.055895747693043 30.53860280530586 5 24.180667610486637 22.106879083016356 29.972424537044933 23.740028769452074 6 29.852684485532833 24.202841694099988 22.57890278085751 23.365571039509668 7 33.83856129996987 20.605636765767763 21.69375884831221 23.86204308595016 8 30.302420386511336 19.880942227983695 24.67975506166101 25.136882323843963 9 25.51998226073311 22.242084136433157 27.035097594396213 25.202836008437522 10 30.966505762418905 19.36059039919036 24.362836235864023 25.310067602526708 11 30.055207782534783 21.268243869434446 25.593099880032522 23.08344846799825 12 28.00097793394397 18.616223469277525 27.706233191760337 25.676565405018163 13 24.97404495084745 17.235858336034024 27.698045837810792 30.092050875307734 14 22.755613170268536 22.800871043489632 22.94642400259266 31.49709178364917 15 23.49122417998533 23.776303295978533 27.06784701019439 25.66462551384174 16 29.882477356849236 24.31928406138241 22.342834075312286 23.45540450645607 17 28.496199134642175 22.610287637664104 20.301226397393695 28.592286830300033 18 27.436164224674638 20.930060666018502 20.83465524985644 30.799119859450425 19 26.330302875239507 23.357156259061522 24.788351214741784 25.52418965095718 20 33.20824875910417 18.092232816506616 21.255280559014334 27.44423786537488 21 28.227608439797365 19.79872754874034 25.603561498968052 26.370102512494242 22 27.85474269534515 23.01760849665399 24.16668088082283 24.960967927178036 23 29.103427885900125 19.78235284084125 22.90332668110825 28.210892592150373 24 29.977200493515504 19.952467861792915 22.167033391895657 27.903298252795928 25 25.965337966818492 23.483179055184856 26.461015779667267 24.09046719832939 26 27.02825763327698 20.040463077441835 26.672026571671715 26.259252717609467 27 27.022146370151695 23.28891758967233 23.383476420832924 26.305459619343047 28 25.557991463955034 19.26090911211139 27.014133451500804 28.166965972432774 29 33.33392542698834 20.200459227838824 23.2011898714091 23.26442547376374 30 25.88764001437503 21.90195696444842 23.306426534274404 28.903976486902145 31 26.791572102697685 20.53078938032454 29.429603098620298 23.248035418357478 32 27.804152347106147 20.076959501679294 25.396757595875968 26.72213055533859 33 29.035345267037883 21.4535869735707 23.082625872480588 26.428441886910832 34 23.309525070088885 23.421815034296458 28.827123936354244 24.441535959260413 35 25.014465660746872 23.81035395122723 24.58210313397922 26.593077254046676 36 26.372448428890987 22.4809961998603 27.19091142560448 23.95564394564423 37 24.41225175870012 23.14175434227728 23.227666064869577 29.218327834153023 38 26.140836273928116 26.66533429097836 21.879274271617273 25.31455516347625 39 26.42747469683201 27.879046156738635 20.23338451414784 25.460094632281518 40 25.636729391267565 28.70733107410634 20.705916119903524 24.950023414722576 41 27.790417525016576 24.02515070684166 21.404673810162524 26.77975795797924 42 28.689484204076276 26.00470584638354 21.738105763069424 23.567704186470756 43 23.714158356445232 22.92130992126753 27.597471414679514 25.76706030760772 44 25.04974116471348 23.41815329210782 25.349029578031818 26.18307596514688 45 26.458733630715724 24.748480716637687 25.28684539157231 23.50594026107428 46 26.516174808819276 26.5812156728773 23.662654718419045 23.239954799884373 47 34.14761754134484 21.447800258125824 20.521937358441207 23.882644842088123 48 24.739988546366266 25.359043481904653 21.27470562764516 28.62626234408392 49 25.871757925072046 23.89242285794616 22.38788922471357 27.847929992268224 50 25.775813816467814 29.079687730151715 21.61702754455737 23.527470908823094 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 532.0 1 594.0 2 779.0 3 863.0 4 794.0 5 612.5 6 434.5 7 413.0 8 420.5 9 403.0 10 375.0 11 382.0 12 394.0 13 419.0 14 466.0 15 540.0 16 601.0 17 758.5 18 922.5 19 1132.5 20 1395.5 21 1842.0 22 2277.0 23 2765.0 24 3250.0 25 3728.0 26 4370.0 27 5161.5 28 6012.5 29 6832.5 30 7643.5 31 8805.0 32 10015.0 33 11343.5 34 12989.5 35 14493.0 36 16544.0 37 18947.0 38 22603.5 39 31499.0 40 40085.0 41 44417.5 42 50660.0 43 64529.0 44 69814.5 45 55338.5 46 45752.5 47 44889.5 48 45401.0 49 48601.5 50 55543.0 51 64904.0 52 69389.5 53 69419.0 54 66068.5 55 58031.0 56 51721.0 57 51611.5 58 54248.5 59 55458.5 60 53480.0 61 49506.0 62 46213.0 63 44328.0 64 46357.0 65 48706.5 66 42466.5 67 35696.0 68 31447.0 69 28267.5 70 25459.5 71 22795.5 72 22288.0 73 19583.5 74 15749.0 75 14070.0 76 12996.5 77 9347.5 78 6372.5 79 6838.0 80 7034.5 81 4426.5 82 2293.0 83 2171.5 84 2015.0 85 1681.0 86 1274.5 87 751.0 88 359.0 89 253.0 90 185.0 91 108.5 92 59.5 93 43.0 94 30.5 95 16.0 96 11.5 97 10.0 98 13.0 99 16.0 100 16.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 24 4038.0 25 3461.0 26 4721.0 27 9645.0 28 13079.0 29 18028.0 30 3357.0 31 3684.0 32 3328.0 33 3881.0 34 3371.0 35 2789.0 36 2873.0 37 2575.0 38 2750.0 39 3453.0 40 4096.0 41 5295.0 42 6522.0 43 5960.0 44 5361.0 45 6078.0 46 11813.0 47 33317.0 48 146850.0 49 229630.0 50 339450.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 17.77514206291712 #Duplication Level Percentage of deduplicated Percentage of total 1 56.134479658883784 9.977983505645906 2 9.26008768677247 3.291987482949004 3 5.48505543563487 2.924929187741567 4 4.273233095129362 3.0382930133553367 5 3.604808329792133 3.2037990085821075 6 2.980941631136879 3.179199658483314 7 2.5736722570606085 3.2023172994848608 8 2.143242549716173 3.0477152637194944 9 1.7618356708944578 2.8185191407497494 >10 11.103328629485397 33.33077638280637 >50 0.3962126310195196 4.840893022421517 >100 0.22491278064474327 7.891350602786416 >500 0.03007554624900637 3.781693449598981 >1k 0.02419120024376599 8.695803032353846 >5k 0.0032690811140224313 4.01761347733979 >10k+ 6.538162228044862E-4 2.7571264719817523 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA 23724 2.6977331263752196 No Hit AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA 8383 0.9532581688755466 No Hit CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC 7383 0.8395449195763044 No Hit CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGC 6640 0.7550559753469676 No Hit ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA 6217 0.706955270893388 No Hit CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGG 5947 0.6762526935825928 No Hit CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGC 4475 0.5088667906141084 No Hit CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGA 3910 0.4446188047600367 No Hit CCCTGTAATTGGAATGAGTCCACTTTAAATCCTTTAACGAGGATCCAT 3559 0.4047054542560026 No Hit CAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGCGC 3232 0.36752122173515045 No Hit ATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGC 2717 0.3089588983460408 No Hit CGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGT 2711 0.3082766188502453 No Hit AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG 2631 0.299179558906306 No Hit AGGCCTGGTTTGCGCTGGAGAGTGGTCTTGGTTGTGAGGGTCATAAGAT 2511 0.2855339689903969 No Hit CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA 2449 0.2784837475338439 No Hit ATCAGATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGG 2373 0.2698415405871015 No Hit CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGAC 2312 0.26290503237984775 No Hit ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGT 2273 0.25847021565717726 No Hit CAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGG 2200 0.2501691484583326 No Hit TCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA 2157 0.24527947873846523 Illumina Single End Adapter 1 (100% over 31bp) AACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTT 2129 0.24209550775808641 No Hit ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA 2029 0.23072418282816223 Illumina Single End Adapter 1 (100% over 32bp) ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA 1950 0.2217408361335221 Illumina Single End Adapter 1 (100% over 32bp) CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCG 1901 0.21616888691785927 No Hit AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAAG 1862 0.21173407019518878 No Hit ACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTCG 1824 0.20741296672181758 No Hit CTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAATCCT 1791 0.2036604294949426 No Hit CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCC 1787 0.20320557649774565 No Hit CCCTTCCACCTTCTGCCGTGTTAGGATTTGGTGTTCCTCCCTTCCAGA 1786 0.20309186324844636 No Hit ATCCGAATTCAGTCTTCCTGAGGCTCTGGCTCACCTCCAGGCAGGTCCC 1770 0.2012724512596585 No Hit TCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA 1684 0.1914931118199237 Illumina Single End Adapter 1 (100% over 31bp) ATTAGGCCTGGTTTGCGCTGGAGAGTGGTCTTGGTTGTGAGGGTCATA 1570 0.1785298013998101 No Hit AGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTCGCT 1431 0.16272365974721545 No Hit ACGCACGGCCGGTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATG 1347 0.1531717468060791 No Hit ATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAAT 1331 0.15135233481729124 No Hit AAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCGAATG 1238 0.14077700263246173 No Hit CGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTG 1196 0.13600104616189357 No Hit CTTCCGCCACGGCCGTCTCTGGAGAGCAGCAGCCATGGCCCTACGCTAC 1151 0.13088394994342764 No Hit CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCC 1147 0.1304290969462307 No Hit CGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGGGGAATCA 1132 0.12872339820674206 No Hit ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAAT 1103 0.12542571397706404 No Hit TCTGGAAGAGGTCTTGCGAAAGCCGCCTCGAGCGCGCTCCGCGGCTGC 1084 0.12326516224037842 No Hit TACCTGGTTGATCCTGCCAGTAGCATATG 1071 0.12178688999948828 No Hit CACGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAA 992 0.11280354330484817 No Hit CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC 946 0.10757273383708303 No Hit CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTAG 924 0.1050710423524997 No Hit TCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA 919 0.1045024761060035 Illumina Single End Adapter 1 (100% over 31bp) TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAAT 904 0.10279677736651485 No Hit CTCTCTCCTCCCGCCGCCCAAGATGCCGAAAGGAAAGAAGGCCAAGGGA 884 0.10052251238053002 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.22799506484498042 0.0 0.0 0.0 0.0 2 0.3819628043961542 0.0 0.0 0.0 0.0 3 0.45633126943785857 0.0 0.0 0.0 0.0 4 0.5479841483730477 0.0 0.0 0.0 0.0 5 0.6530551907255474 0.0 0.0 0.0 0.0 6 0.7841665671675735 0.0 0.0 0.0 0.0 7 0.8636521284277437 0.0 0.0 0.0 0.0 8 0.9417731306963231 0.0 0.0 0.0 0.0 9 1.0007903070826298 0.0 0.0 0.0 0.0 10 1.0524161222644857 0.0 0.0 0.0 0.0 11 1.1066573421802242 0.0 0.0 0.0 0.0 12 1.1427044422080839 0.0 0.0 0.0 0.0 13 1.182162939714921 0.0 0.0 0.0 0.0 14 1.2229859962133487 0.0 0.0 0.0 0.0 15 1.2702907079218335 0.0 0.0 0.0 0.0 16 1.3022441309749206 0.0 0.0 0.0 0.0 17 1.3513682546721932 0.0 0.0 0.0 0.0 18 1.3774085887617196 0.0 0.0 0.0 0.0 19 1.4215293294898255 0.0 0.0 0.0 0.0 20 1.4426799938594845 0.0 0.0 0.0 0.0 21 1.4739511374167762 0.0 0.0 0.0 0.0 22 1.4929412500497496 0.0 0.0 0.0 0.0 23 1.515229046912401 0.0 0.0 0.0 0.0 24 1.5366071377806585 0.0 0.0 0.0 0.0 25 1.556620669657325 0.0 0.0 0.0 0.0 26 1.586072401225829 0.0 0.0 0.0 0.0 27 1.6056310801052984 0.0 0.0 0.0 0.0 28 1.6217783615057908 0.0 0.0 0.0 0.0 29 1.640199907892268 0.0 0.0 0.0 0.0 30 1.6541866375560748 0.0 0.0 0.0 0.0 31 1.66692252147759 0.0 0.0 0.0 0.0 32 1.6788624126540104 0.0 0.0 0.0 0.0 33 1.6936451350629118 0.0 0.0 0.0 0.0 34 1.706949585230923 0.0 0.0 0.0 0.0 35 1.718662049908745 0.0 0.0 0.0 0.0 36 1.7281002496005822 0.0 0.0 0.0 0.0 37 1.741177273269995 0.0 0.0 0.0 0.0 38 1.7534583041943133 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATTAGTC 805 0.0 97.84766 44 TAAGCCA 2775 0.0 96.202965 44 CTTATCG 15 0.0033044915 63.34778 43 TATTAGT 800 0.0 59.388542 43 TTAAGCC 2790 0.0 58.12556 43 CGACGGG 1255 0.0 57.286884 44 CTATCAT 170 0.0 55.973648 44 CAATGAA 1370 0.0 53.17514 43 CGCGAAC 70 3.4009645E-8 52.864002 44 ATAAGAT 485 0.0 47.674095 43 ATTAAGC 2790 0.0 46.516438 42 AGGTCCC 360 0.0 45.75117 43 ATATTAG 850 0.0 45.44855 42 AATGAAG 565 0.0 43.975365 44 CATATTA 835 0.0 43.882324 41 GCAATGA 1360 0.0 43.257812 42 AGATTAA 2845 0.0 43.25148 40 GCATATT 845 0.0 43.209568 40 GATTAAG 2925 0.0 43.020668 41 AAGATTA 2905 0.0 42.086094 39 >>END_MODULE