FastQCFastQC Report
Tue 24 May 2016
ERR841152.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841152.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences214435
Sequences flagged as poor quality0
Sequence length24-50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA27811.2968964954415092No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTACCTGAGGGAAGCAGTGGT13780.6426189754471052No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC12820.5978501643854781No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA10870.5069135169165482No Hit
AAGCAGTGGTATCAACGCAGAGTGCAG8000.3730734255135589No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA7650.3567514631473407Illumina Single End Adapter 1 (100% over 32bp)
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA7470.3483573110732856Illumina Single End Adapter 1 (100% over 32bp)
AGGCCTGGTTTGCGCTGGAGAGTGGTCTTGGTTGTGAGGGTCATAAGAT7220.3366987665259869No Hit
AAGCAGTGGTATCAACGCAGAGTG7020.3273719308881479No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA6660.31058362674003775No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTG6380.29752605684706324No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA5700.2658148156784107No Hit
CTTCCGCCACGGCCGTCTCTGGAGAGCAGCAGCCATGGCCCTACGCTAC5650.263483106768951No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTACCTG5440.2536899293492201No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTACC5030.23456991629165017No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG4980.2322382073821904No Hit
CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGG4580.21358453610651248No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGC4070.18980110523002308No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTACCTGAGGG3900.18187329493785995No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGGTTGTGAGGGTTCCTGCTT3850.1795415860284002No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCC3800.17720987711894048No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA3520.1641523072259659Illumina Single End Adapter 1 (100% over 32bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC3450.16088791475272227No Hit
ATTAGGCCTGGTTTGCGCTGGAGAGTGGTCTTGGTTGTGAGGGTCATA3210.1496957119873155No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTA3110.14503229416839603No Hit
TCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA2910.13570545853055704Illumina Single End Adapter 1 (100% over 31bp)
AAGCAGTGGTATCAACGCAGAGTGCAGT2820.1315083824935295No Hit
CGCAATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAG2760.1287103318021778No Hit
TGGAGAAGGGTTCCATGTGAACAGCAGTTGAACATGGGTCAGTCGGTCC2690.12544593932893416No Hit
AGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA2680.12497959754704223Illumina Single End Adapter 2 (100% over 34bp)
AAGCAGTGGTATCAACGCAGAGTGCAGTGCT2640.12311423041947442No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAAG2550.11891715438244689No Hit
ATCAAAAGGGACAAGCATCAAGCACGCAGCAATGCAGCTCAAAACGCTT2530.11798447081866299No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT2480.11565276190920325No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTCCT2380.11098934409028377No Hit
ACAACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAAT2360.11005666052649989No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA2290.10679226805325624Illumina Single End Adapter 1 (100% over 32bp)
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTACCTGAGGGAAGCAGTGG2250.10492690092568843No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTACCT2240.10446055914379648No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA2220.1035278755800126Illumina Single End Adapter 1 (100% over 32bp)
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGT2220.1035278755800126No Hit
AGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA2180.10166250845244479Illumina Single End Adapter 2 (100% over 34bp)
TCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA2180.10166250845244479Illumina Single End Adapter 1 (100% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGCCA1050.087.1162144
CGCCTAA250.003054241464.5684944
GTAGAGA351.4970452E-461.49379744
GAACACA1050.061.49379344
ACACCTT300.00628656253.8070744
ACTAACG203.343261E-452.29846242
CAATGAA4350.051.7350243
ACGCTAC1100.051.14712543
AATCCCG254.166759E-450.0105243
GAGTATT254.166759E-450.0105243
GGCTTAC150.00940443948.61939639
TCGTGCG651.0913936E-1148.0870443
ACGTCCC205.3683406E-447.51410737
ATAAGAT1450.047.42377543
TACGCTA1000.047.06861542
AACGCTT401.6220511E-646.88486543
GACAATT205.879863E-446.64533635
CGCTACT253.399962E-546.0143233
GACGCTA206.5558834E-445.62703731
CGACGTT302.0431762E-645.35267630
Produced by FastQC (version 0.11.5)