FastQCFastQC Report
Tue 24 May 2016
ERR841151.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841151.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4372566
Sequences flagged as poor quality0
Sequence length24-50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA1772344.053317891599578No Hit
CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGG360990.8255793051494249No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGC343400.7853512102504571No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGC293100.6703157825405036No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC254610.5822896669827282No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA223310.5107069853262364No Hit
CGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGT219680.5024052238433908No Hit
ATCCGAATTCAGTCTTCCTGAGGCTCTGGCTCACCTCCAGGCAGGTCCC200720.459043957255305No Hit
ATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGC184800.4226351300357731No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCC179560.41065132007155525No Hit
CAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGCGC169590.3878500633266599No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCC168460.38526576842979615No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCG154520.3533851747463618No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA151910.34741613963059675No Hit
CTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAATCCT131350.3003956944274826No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA128270.2933517755935531Illumina Single End Adapter 1 (100% over 32bp)
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA122090.2792181981930061Illumina Single End Adapter 1 (100% over 32bp)
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTTTCATAGGGAAGCAGTGGT114000.2607164763207691No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGA106930.24454748081561262No Hit
ACTACTTCTCCCCCGGACTCCTTGGTAGTCTGTTAGTGGGAGATCCTTG105710.24175735712165353No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAAG100360.2295219786276525No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGAC99000.22641167680487845No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCCG95400.21817852492106463No Hit
CGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTG94710.21660050414333368No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAAT91400.20903057838349381No Hit
TCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGC89910.20562296829824867No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATC86570.197984432939377No Hit
TACCTGGTTGATCCTGCCAGTAGCATATG83420.19078042504103998No Hit
CGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGGGGAATCA80400.183873725405174No Hit
AGGCCTGGTTTGCGCTGGAGAGTGGTCTTGGTTGTGAGGGTCATAAGAT78510.17955132066617174No Hit
CTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAATCCTG76830.175709183120392No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA70070.16024915347189728No Hit
TCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA67780.15501195407913798Illumina Single End Adapter 1 (100% over 31bp)
CTCTCTCCTCCCGCCGCCCAAGATGCCGAAAGGAAAGAAGGCCAAGGGA63560.14536087048200075No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTAG60020.13726493779625054No Hit
CCCTTCCACCTTCTGCCGTGTTAGGATTTGGTGTTCCTCCCTTCCAGA58870.1346349031666989No Hit
AAGCAGTGGTATCAACGCAGAGTGCAG55370.1266304499463244No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA54820.12537260729740843Illumina Single End Adapter 1 (100% over 32bp)
ATCCGAATTCAGTCTTCCTGAGGCTCTGGCTCACCTCCAGGCAGGTCCCA54720.12514390863396918No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG52480.12002105857292948No Hit
CAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGG52360.11974662017680236No Hit
TCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA52160.11928922284992383Illumina Single End Adapter 1 (100% over 31bp)
CTTCCGCCACGGCCGTCTCTGGAGAGCAGCAGCCATGGCCCTACGCTAC49470.11313722880340743No Hit
TTGTCTTTCTAGGTCTCAGCCGGTCGTCGCGACGTTCGCCCGCTCGCT48400.11069015310460724No Hit
TCGCTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAAT47180.10790002941064812No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCCC46690.1067794059597957No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTG46510.106367748365605No Hit
AAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCGAATG46120.10547582357819184No Hit
ATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGGGGAATCAG46090.10540721397916007No Hit
ATCAGATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGG45810.1047668577215301No Hit
AACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTT44300.10131350790359712No Hit
CGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTC43770.10010140498736897No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGCCA208300.0114.00799644
ATTAGTC30500.0112.5113744
CGACGGG68900.066.81866544
TTAAGCC206550.060.96089643
TATTAGT30700.059.81074543
AATGAAG26750.056.0816744
CAATGAA44400.050.6336743
AGGTCCC38050.049.8518843
CTATCAT5100.048.8271644
ATTAAGC206450.048.4414542
ATATTAG30900.047.4749942
AATCCCG14550.047.40678843
GATTAAG213400.045.58551441
CCAGAAT13750.045.49695244
AGATTAA209750.045.31560540
CATATTA31050.045.01339741
AAGATTA212950.044.3656439
ATCCTTG19200.044.2415343
AAAGATT212200.044.15997338
GCATATT31550.043.76144440
Produced by FastQC (version 0.11.5)