FastQCFastQC Report
Tue 24 May 2016
ERR841150.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841150.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4317086
Sequences flagged as poor quality0
Sequence length24-50
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA2077434.812111688300859No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGC346970.8037134307725164No Hit
CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGG273960.6345947243117233No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGC230490.5339018032070707No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAAG212550.4923459944972141No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCG199580.462302580953912No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC195370.45255063253314853No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA180180.41736486139029894No Hit
CGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGT170360.3946180363328412No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCC168440.3901705919224217No Hit
ATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGC161670.37448871762109903No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCCG160770.37240397805371495No Hit
CTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAATCCTG154800.358575205590067No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGAC147740.3422215818725872No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA140860.32628490606858424Illumina Single End Adapter 1 (100% over 32bp)
ATCCGAATTCAGTCTTCCTGAGGCTCTGGCTCACCTCCAGGCAGGTCCC136840.31697306933426855No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA135550.31398494262101795Illumina Single End Adapter 1 (100% over 32bp)
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA134990.31268777133464565No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCC134050.3105103766753778No Hit
CTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAATCCT132520.3069663194108248No Hit
CAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGCGC128320.2972375347630323No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAAT110040.2548941577721639No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATC96520.22357673671546036No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTAG95760.22181628996966934No Hit
TACCTGGTTGATCCTGCCAGTAGCATATG94580.21908296475909908No Hit
TCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGC86980.2014784973011888No Hit
ATCCGAATTCAGTCTTCCTGAGGCTCTGGCTCACCTCCAGGCAGGTCCCA86080.19939375773380472No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGA72750.16851644836354893No Hit
AGGCCTGGTTTGCGCTGGAGAGTGGTCTTGGTTGTGAGGGTCATAAGATG70930.16430064168283884No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA68200.1579769316617737No Hit
CGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTG67070.1553594253160581No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA64100.14847978474369053Illumina Single End Adapter 1 (100% over 32bp)
CGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTC62340.14440296070080605No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG61620.14273516904689876No Hit
TCGCTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAATC60730.14067359325248557No Hit
ATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGGGGAATCAG60580.14032613665792157No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAAT60050.13909845669046203No Hit
AGGCCTGGTTTGCGCTGGAGAGTGGTCTTGGTTGTGAGGGTCATAAGAT59090.13687473448525234No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG58850.13631880393394988No Hit
CAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGG57340.13282107421533879No Hit
AAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCGAATG55700.1290222154481055No Hit
CGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGGGGAATCA55270.1280261732103553No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA52670.12200359223791234Illumina Single End Adapter 1 (100% over 32bp)
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCGC52110.12070642095154No Hit
CTGTGCGTCTGCTCCTCCTTCTCGCGCTTCTCCTGCCGCCCTAATCCTG51020.11818156969770813No Hit
AACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTT50570.11713919991401607No Hit
TCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA50480.11693072595727766Illumina Single End Adapter 1 (100% over 31bp)
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGT48550.11246011777388729No Hit
ATCAGATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGG47360.10970362879034609No Hit
ACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTCG46700.10817481977426441No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA46230.10708612244463049Illumina Single End Adapter 1 (100% over 32bp)
AGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTCGCT45920.1063680454825315No Hit
CTGTGCGTCTGCTCCTCCTTCTCGCGCTTCTCCTGCCGCCCTAATCCT45670.10578895115825815No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGCCA237550.0102.1002344
ATTAGTC24250.092.3637644
AATGAAG36100.067.5899944
CTATACG1050.066.9833844
TTAAGCC237500.058.1793143
CGACGGG58800.054.83804344
CGCTACC6600.053.28223444
TATTAGT24400.052.14221243
CCAGAAT24950.051.8251344
CAATGAA49650.051.7423343
AATCCCG23250.050.643543
CGTGCGC12300.049.26357744
ATTAAGC237750.048.30287642
ATAAGAT17400.047.98431843
AATCCTG37400.047.6741443
TCGTGCG17750.046.69354643
AGGTCCC28900.046.4571543
GGTCCCA19500.046.33347744
AGATTAA240600.045.6030340
GATTAAG245450.045.59517741
Produced by FastQC (version 0.11.5)