##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841149.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3856529
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.65467807969291	37.0	35.0	39.0	31.0	39.0
2	36.678717572200284	39.0	35.0	39.0	33.0	39.0
3	36.92105543611885	39.0	35.0	39.0	33.0	39.0
4	37.345358222380796	39.0	35.0	40.0	34.0	41.0
5	37.869915408389254	40.0	35.0	41.0	34.0	41.0
6	37.87495802572728	40.0	35.0	41.0	34.0	41.0
7	37.749860042540845	40.0	35.0	41.0	34.0	41.0
8	37.73571960693152	39.0	35.0	41.0	34.0	41.0
9	37.78442091320978	39.0	35.0	41.0	34.0	41.0
10	37.69608292845717	39.0	35.0	41.0	34.0	41.0
11	37.76511028440341	39.0	35.0	41.0	34.0	41.0
12	37.729677645364525	39.0	35.0	41.0	34.0	41.0
13	37.71279147648053	39.0	35.0	41.0	34.0	41.0
14	37.7214881568374	39.0	35.0	41.0	34.0	41.0
15	37.73535762339658	39.0	35.0	41.0	34.0	41.0
16	37.676367790829524	39.0	35.0	41.0	34.0	41.0
17	37.60653426954653	39.0	35.0	41.0	34.0	41.0
18	37.58252018849074	39.0	35.0	41.0	34.0	41.0
19	37.37929028927307	38.0	35.0	41.0	33.0	41.0
20	37.37852794572529	38.0	35.0	41.0	33.0	41.0
21	37.09006648206198	38.0	35.0	40.0	33.0	41.0
22	36.964887597111286	38.0	35.0	40.0	33.0	41.0
23	36.87881149085097	38.0	35.0	40.0	33.0	41.0
24	36.77686904467722	38.0	35.0	40.0	33.0	41.0
25	36.704249978554685	38.0	35.0	40.0	33.0	41.0
26	36.56028691432926	37.0	35.0	40.0	33.0	41.0
27	36.49945951938801	37.0	35.0	40.0	33.0	41.0
28	36.65113901520787	37.0	35.0	40.0	33.0	41.0
29	36.69135744407772	37.0	35.0	40.0	33.0	41.0
30	36.68380685746752	36.0	35.0	40.0	33.0	41.0
31	36.62358865911678	36.0	35.0	40.0	33.0	41.0
32	36.63578759965898	36.0	35.0	40.0	33.0	41.0
33	36.58735310841546	36.0	35.0	40.0	33.0	41.0
34	36.489458353345235	35.0	35.0	40.0	33.0	41.0
35	36.37277292060323	35.0	35.0	40.0	33.0	41.0
36	36.27320776922561	35.0	35.0	40.0	33.0	41.0
37	36.23708682972306	35.0	35.0	40.0	33.0	41.0
38	36.14135371745656	35.0	35.0	39.0	33.0	41.0
39	36.01233471983662	35.0	35.0	39.0	33.0	41.0
40	35.872745460476246	35.0	35.0	39.0	33.0	41.0
41	35.71763491483738	35.0	35.0	39.0	33.0	41.0
42	35.63299589154619	35.0	35.0	38.0	33.0	41.0
43	35.58210459864774	35.0	35.0	38.0	33.0	41.0
44	35.523001433323735	35.0	35.0	37.0	33.0	41.0
45	35.412224220385426	35.0	35.0	37.0	33.0	41.0
46	35.30716127197121	35.0	35.0	37.0	33.0	41.0
47	35.260296898878636	35.0	35.0	37.0	33.0	40.0
48	35.202564676738994	35.0	35.0	36.0	33.0	40.0
49	35.443555613447835	35.0	35.0	37.0	32.0	41.0
50	36.21945837082744	36.0	35.0	39.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	5.0
14	254.0
15	765.0
16	103.0
17	2.0
18	0.0
19	1.0
20	13.0
21	50.0
22	134.0
23	362.0
24	949.0
25	2135.0
26	4351.0
27	8467.0
28	15420.0
29	27614.0
30	46979.0
31	78411.0
32	130790.0
33	253834.0
34	755362.0
35	235659.0
36	291339.0
37	470630.0
38	530843.0
39	548938.0
40	453119.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	43.33399282100562	20.02811336307856	36.63789381591581
2	28.41726329556967	21.005339257140292	28.538045480793738	22.039351966496298
3	33.76302887907753	12.6456458644548	21.127703175575757	32.463622080891916
4	20.155352131411433	17.125736640383103	26.82145006559007	35.89746116261539
5	28.497387158245147	17.740097377719703	31.659245917766988	22.10326954626816
6	33.5109369072552	21.490179381511197	19.949078562614204	25.04980514861939
7	36.12414168284486	18.774032296917774	21.447005843855965	23.654820176381406
8	31.082276316345602	14.932365347181364	28.702986545673582	25.282371790799445
9	25.4260242824571	20.974741795018268	27.62782284276872	25.971411079755914
10	33.36839940786132	16.98410669283182	22.465304941308624	27.182188957998243
11	32.35562341162221	21.65776531176091	23.570780875756412	22.41583040086046
12	27.252926141615948	14.937551357710522	26.384399028245348	31.425123472428183
13	23.382787994074462	15.711485639029293	29.18595970625399	31.71976666064225
14	23.75908491807011	20.78783797554744	19.942388609031592	35.510688497350856
15	25.258671722681196	18.660069715539542	27.48437779153223	28.59688077024703
16	30.115578023658063	20.795616991341177	24.68489151773525	24.40391346726551
17	27.345729800035212	17.207182935743514	20.564606152319868	34.882481111901406
18	28.308720095194413	19.745786949871245	18.043764224254506	33.90172873067984
19	26.131581014948935	24.098872327940487	27.045874671239346	22.72367198587123
20	36.75776844929729	15.213524908019622	20.191861645536697	27.836844997146397
21	29.155336314079317	17.813686867128446	24.55259638913645	28.478380429655786
22	27.842160657938784	22.03712198196876	25.87591069586149	24.24480666423097
23	33.37392250907487	17.351172518085566	20.658239572423803	28.616665400415762
24	32.79215584791402	18.2502452334729	19.039633826168554	29.917965092444526
25	27.941768871940063	21.427556431155853	25.20435622736672	25.426318469537357
26	31.15882592794857	16.435179604470797	26.70102503844287	25.70496942913776
27	26.864064176628272	23.705783208541046	22.58741729458767	26.842735320243012
28	28.02040403309356	16.054639491668308	27.817114244625913	28.107842230612214
29	39.3170794662566	17.47421500723355	22.386987208740525	20.821718317769328
30	26.58373977360658	21.461270686856935	19.91052331811078	32.04446622142571
31	26.000292437798954	17.350479992194202	34.46558390127121	22.18364366873563
32	28.12291477941191	16.028726602368767	25.580156265395722	30.268202352823604
33	31.796133208780102	18.547544586447167	21.114706307086898	28.541615897685833
34	23.122383349493887	19.863435764061947	30.86347767578001	26.150703210664155
35	26.54367745112066	21.19325404399247	21.721321850572597	30.541746654314274
36	25.150375563988703	18.546305275892063	29.124540989249397	27.178778170869833
37	25.60428020885025	18.104569036371053	22.747826196641803	33.543324558136895
38	29.56412336004295	24.64743854395722	19.62364098732456	26.16479710867527
39	25.792657500574663	26.673044735811636	18.87344393786956	28.66085382574414
40	26.787401688956543	26.85852633104998	18.358160269653148	27.995911710340327
41	31.066106974194614	20.09414227769438	20.07401595076943	28.76573479734158
42	29.381037860441417	22.91132123540591	22.29174414552653	25.415896758626143
43	23.627000112339772	21.92624672821471	26.94403091024406	27.50272224920146
44	26.754219105641596	20.775033459058278	25.117054771959086	27.35369266334104
45	25.9423555627082	23.794132725344987	28.33365231621883	21.929859395727977
46	27.374772179655626	26.283732837283225	21.24623560273249	25.09525938032866
47	35.98817693461595	17.077332605282916	18.40536145468881	28.529129005412322
48	23.69108437478367	20.366936756369036	22.226483394374625	33.715495474472675
49	28.769581261086103	19.562378102032806	18.3674611215968	33.30057951528429
50	25.22063303095849	35.34003662778458	18.364940167787296	21.074390173469634
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1078.0
1	1281.5
2	2509.5
3	3412.0
4	3114.0
5	2094.5
6	1038.0
7	875.5
8	988.0
9	1058.0
10	1078.5
11	1085.0
12	1050.5
13	1036.5
14	1050.5
15	1138.5
16	1262.0
17	1663.0
18	2025.5
19	2386.5
20	2838.5
21	3506.0
22	4254.0
23	5359.5
24	6475.5
25	7521.0
26	9033.0
27	10687.0
28	12257.5
29	14408.5
30	16984.0
31	19820.5
32	23571.0
33	28686.0
34	33377.5
35	36201.5
36	41935.5
37	48883.0
38	59452.5
39	81529.5
40	101524.0
41	116327.5
42	144744.0
43	254629.0
44	330781.5
45	249465.5
46	183644.5
47	166581.0
48	159956.5
49	183202.0
50	214011.5
51	237737.5
52	250929.5
53	272445.0
54	280598.5
55	266999.5
56	258215.0
57	269672.5
58	306411.5
59	313677.0
60	267360.5
61	234255.5
62	228598.0
63	225922.0
64	276613.5
65	331854.5
66	268579.0
67	191119.5
68	163574.5
69	145252.5
70	131168.5
71	136613.0
72	154900.5
73	131133.0
74	91400.0
75	79620.5
76	76263.0
77	56079.0
78	39829.5
79	36190.0
80	32454.5
81	23997.5
82	16749.5
83	15267.0
84	13785.5
85	11047.0
86	8639.0
87	4901.5
88	1723.0
89	1166.5
90	804.5
91	470.0
92	283.5
93	232.5
94	163.0
95	66.0
96	30.5
97	27.0
98	32.5
99	38.0
100	38.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	21192.0
25	19285.0
26	27757.0
27	57660.0
28	73372.0
29	63254.0
30	17180.0
31	14556.0
32	16801.0
33	14240.0
34	15585.0
35	14912.0
36	12494.0
37	12464.0
38	12869.0
39	14033.0
40	15561.0
41	18404.0
42	23413.0
43	21707.0
44	20603.0
45	26942.0
46	55072.0
47	190294.0
48	789105.0
49	1214272.0
50	1073502.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	11.328159503235815
#Duplication Level	Percentage of deduplicated	Percentage of total
1	44.5930717939159	5.051574300207254
2	10.527803481370759	2.3852127411137856
3	7.197162046825981	2.445917989112396
4	5.647650759699512	2.5590995449778804
5	4.683469755244967	2.6527546207997896
6	3.7868533243209552	2.5738847083959944
7	3.2682758993694034	2.5916485482046663
8	2.7540077386401447	2.4958271149169065
9	2.2967653224525586	2.3416311522819084
>10	14.044281156581834	27.561741438360833
>50	0.673613730589924	5.229131016655004
>100	0.4314676788767664	9.727542947920158
>500	0.04766888498784342	3.7124643322525928
>1k	0.038805825970003184	8.831589869052408
>5k	0.004072216305494161	3.149859549007145
>10k+	0.0050303848479633755	16.69012012674136
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA	172099	4.462536130287105	No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGC	47733	1.2377192029412978	No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGC	42426	1.100108413550112	No Hit
CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGG	39737	1.0303825019855937	No Hit
ATCCGAATTCAGTCTTCCTGAGGCTCTGGCTCACCTCCAGGCAGGTCCC	33248	0.8621223903670893	No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA	32120	0.8328732909826426	No Hit
CTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAATCCT	27924	0.7240707900809251	No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCC	25463	0.660256930519646	No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCG	21873	0.5671680415212748	No Hit
CGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGT	21294	0.5521545410393647	No Hit
CTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAATCCTG	16827	0.43632499587063905	No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAAT	15490	0.40165651548322334	No Hit
ATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGC	15110	0.3918030954778247	No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGAC	14736	0.3821052557883008	No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCC	14573	0.3778786572070377	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA	14482	0.3755190224162712	No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGA	13722	0.35581218240547396	No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCCG	12916	0.33491255997297054	No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG	11843	0.3070896134840423	No Hit
TCGCTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAAT	10771	0.27929259704775977	No Hit
AGGCCTGGTTTGCGCTGGAGAGTGGTCTTGGTTGTGAGGGTCATAAGAT	10674	0.27677738194111856	No Hit
ATCCGAATTCAGTCTTCCTGAGGCTCTGGCTCACCTCCAGGCAGGTCCCA	8894	0.23062188823161966	No Hit
CTCTGCAGGTACCATGGAGCTGAGCTATAGGCTCTTCATCTGCCTCCT	8363	0.21685303027670738	No Hit
TACCTGGTTGATCCTGCCAGTAGCATATG	8117	0.21047423732584403	No Hit
CAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGCGC	8020	0.20795902221920284	No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGT	7785	0.20186545984744314	No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAAG	7381	0.19138971857854564	No Hit
ACTACTTCTCCCCCGGACTCCTTGGTAGTCTGTTAGTGGGAGATCCTTG	7311	0.18957461489334063	No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATC	7042	0.1825994307316242	No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA	6819	0.17681702899161397	Illumina Single End Adapter 1 (100% over 32bp)
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA	6415	0.16634128772271645	Illumina Single End Adapter 1 (100% over 32bp)
TCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGC	6142	0.15926238335041693	No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA	6099	0.15814739108664813	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAAT	5911	0.15327254118924039	No Hit
CGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTC	5903	0.15306510076807409	No Hit
AGCGAGCGGCCTTGAGAGGCTCTGGCTCTTGCTTCTTAGGCGGCCCGAG	5657	0.14668630781721076	No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTG	5203	0.13491406391602398	No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC	5016	0.13006514407126202	No Hit
ATTAGGCCTGGTTTGCGCTGGAGAGTGGTCTTGGTTGTGAGGGTCATA	4682	0.12140450648756952	No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGGTTGTGAGGGTTCCTGCTT	4632	0.12010800385528023	No Hit
ATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGGGGAATCAG	4580	0.11875964111769936	No Hit
CTCTTTTTAAGTTAGTGCTGGAACGTGGAAGAGCTGCTGCCTCCGAAG	4443	0.1152072239052267	No Hit
CTCTGCAGGTACCATGGAGCTGAGCTATAGGCTCTTCATCTGCCTCCTG	4413	0.11442932232585312	No Hit
TCTGGAAGAGGTCTTGCGAAAGCCGCCTCGAGCGCGCTCCGCGGCTGC	4359	0.11302909948298068	No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTGAGGAGGGGAAGCAGTGGT	4168	0.10807645942763557	No Hit
CCGATCGCACGCCCCCCGTGGCGGCG	4143	0.10742820811149092	No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTAG	4096	0.10620949563713898	No Hit
TTGTCTTTCTAGGTCTCAGCCGGTCGTCGCGACGTTCGCCCGCTCGCT	4086	0.10595019511068114	No Hit
TCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA	3967	0.10286451884583261	Illumina Single End Adapter 1 (100% over 31bp)
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.18083618715171076	0.0	0.0	0.0	0.0
2	0.24898036550483607	0.0	0.0	0.0	0.0
3	0.2860084806830183	0.0	0.0	0.0	0.0
4	0.330374800759958	0.0	0.0	0.0	0.0
5	0.3913363545302006	0.0	0.0	0.0	0.0
6	0.4515718668263612	0.0	0.0	0.0	0.0
7	0.4982978216940674	0.0	0.0	0.0	0.0
8	0.5487577041427667	0.0	0.0	0.0	0.0
9	0.5851634980574502	0.0	0.0	0.0	0.0
10	0.6182761752861187	0.0	0.0	0.0	0.0
11	0.6589863579400025	0.0	0.0	0.0	0.0
12	0.6862388432707235	0.0	0.0	0.0	0.0
13	0.7159028234975026	0.0	0.0	0.0	0.0
14	0.748185739041506	0.0	0.0	0.0	0.0
15	0.7814021364807577	8.816217899567201E-4	0.0	0.0	0.0
16	0.8057245258625049	8.816217899567201E-4	0.0	0.0	0.0
17	0.8472126100957623	8.816217899567201E-4	0.0	0.0	0.0
18	0.8691235045814514	8.816217899567201E-4	0.0	0.0	0.0
19	0.9082000939186506	8.816217899567201E-4	0.0	0.0	0.0
20	0.925962179981014	8.816217899567201E-4	0.0	0.0	0.0
21	0.9504660797312817	8.816217899567201E-4	0.0	0.0	0.0
22	0.9640793573703192	8.816217899567201E-4	0.0	0.0	0.0
23	0.9833453864861382	8.816217899567201E-4	0.0	0.0	0.0
24	1.0022743249175619	8.816217899567201E-4	0.0	0.0	0.0
25	1.017650846136513	8.816217899567201E-4	0.0	0.0	0.0
26	1.0490262098379137	8.816217899567201E-4	0.0	0.0	0.0
27	1.0639359901092407	8.816217899567201E-4	0.0	0.0	0.0
28	1.0784827496435265	8.816217899567201E-4	0.0	0.0	0.0
29	1.0981117994963865	8.816217899567201E-4	0.0	0.0	0.0
30	1.1123733284515687	8.816217899567201E-4	0.0	0.0	0.0
31	1.1252346345638786	8.816217899567201E-4	0.0	0.0	0.0
32	1.1362549069383376	8.816217899567201E-4	0.0	0.0	0.0
33	1.1485198218397943	8.816217899567201E-4	0.0	0.0	0.0
34	1.1611218274256463	8.816217899567201E-4	0.0	0.0	0.0
35	1.1730755816953535	8.816217899567201E-4	0.0	0.0	0.0
36	1.1863517686499958	8.816217899567201E-4	0.0	0.0	0.0
37	1.2000687664996166	8.816217899567201E-4	0.0	0.0	0.0
38	1.2089368445044755	8.816217899567201E-4	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAAGCCA	19250	0.0	137.34018	44
ATTAGTC	725	0.0	120.67509	44
CGACGGG	6610	0.0	97.656624	44
CTATACG	130	0.0	79.175964	44
GGTCCCA	1900	0.0	72.359695	44
TTAAGCC	19275	0.0	64.74107	43
CCAGAAT	1485	0.0	61.885975	44
AATGAAG	1850	0.0	60.80347	44
CAATGAA	4930	0.0	59.405838	43
AGGTCCC	5400	0.0	58.951443	43
TATTAGT	700	0.0	58.50533	43
ATAAGAT	1835	0.0	55.326405	43
GGCGGGC	4705	0.0	52.19099	44
AATCCTG	2795	0.0	52.084084	43
AATCCCG	1915	0.0	51.03828	43
TCGTGCG	2255	0.0	49.294952	43
ATTAAGC	19230	0.0	48.287872	42
TTTGGAC	2300	0.0	47.133446	43
TAATCCT	6340	0.0	46.940704	42
ATCCTTG	1500	0.0	46.804264	43
>>END_MODULE