FastQCFastQC Report
Tue 24 May 2016
ERR841148.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841148.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences390677
Sequences flagged as poor quality0
Sequence length24-50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC20200.5170511701482299No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA19880.5088602605221193No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA18830.4819838383114439No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA12920.3307079761542144No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC11950.3058792813500667No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTAACCTAGGGAAGCAGTGGT11350.29052132580110934No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA9770.2500787095221884Illumina Single End Adapter 1 (100% over 32bp)
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA9030.2311372310118077Illumina Single End Adapter 1 (100% over 32bp)
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA8130.20810029768837174No Hit
AGGCCTGGTTTGCGCTGGAGAGTGGTCTTGGTTGTGAGGGTCATAAGAT8120.20784433176255576No Hit
TCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA8080.20682046805929194Illumina Single End Adapter 1 (100% over 31bp)
CTTCCGCCACGGCCGTCTCTGGAGAGCAGCAGCCATGGCCCTACGCTAC6370.16305029474476357No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG5820.14897216882488604No Hit
TCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA5810.14871620289907006Illumina Single End Adapter 1 (100% over 31bp)
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGT5730.14666847549254242No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC5630.14410881623438287No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAAG5510.1410372251245914No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTCCT5250.13438211105337658No Hit
AGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA4770.1220957466142107Illumina Single End Adapter 2 (100% over 34bp)
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGC4620.11825625772697139No Hit
AAGCAGTGGTATCAACGCAGAGTG4590.11748835994952352No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA4300.11006534810086083Illumina Single End Adapter 1 (100% over 32bp)
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT4270.10929745032341295No Hit
AAGCAGTGGTATCAACGCAGAGTGCAG4250.10878551847178103No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAAT4230.10827358662014912No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGA4040.10341023402964597No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTG3910.10008267699403856No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTAGTC1950.078.6480444
GACCGTA301.9349046E-458.4242644
TAAGCCA2200.055.76861244
TTAAGCC1700.050.42569743
CGGATAT150.00910403649.02969441
AACGCTT1050.047.85887543
CAATGAA3750.047.2958343
TATTAGT2200.047.02710343
ACCCGAC406.9630914E-944.87405429
ATTAAGC1600.044.5300542
GCTAGAC408.0005673E-443.81819544
AGTCGGT800.042.90098641
GAACACA1950.042.69465344
CCTTACT1950.042.20622327
TCGTGCG1350.041.60281443
CTACGTC256.733389E-540.99718512
GTATCAC256.733389E-540.99718516
TAAGCAT2000.040.98145337
ACCTTAC2000.040.98031626
CACCTTA2000.040.788525
Produced by FastQC (version 0.11.5)