FastQCFastQC Report
Tue 24 May 2016
ERR841147.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841147.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences623945
Sequences flagged as poor quality0
Sequence length24-50
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA284604.561299473511287No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGC101161.6212967489121637No Hit
CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGG67971.089358837718068No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCG51210.8207454182660331No Hit
ATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGC30210.4841772912676598No Hit
CAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGCGC26070.4178252890879805No Hit
ATCCGAATTCAGTCTTCCTGAGGCTCTGGCTCACCTCCAGGCAGGTCCC24950.39987498898140056No Hit
TCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGC24760.3968298487847487No Hit
TCGGAAGAGCTCGTATGCCGTCTTCT23600.37823846653150517Illumina Single End Adapter 1 (100% over 26bp)
AACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTT23550.37743711384817574No Hit
CTCCTACTTGGATAACTGTGGTAATT19410.3110851116684964No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA19400.3109248411318305No Hit
TCGGAAGAGCTCGTATGCCGTCTT18620.2984237392718909Illumina Single End Adapter 1 (100% over 24bp)
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGC18500.2965004928319003No Hit
ATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGG17870.28640344902194903No Hit
TCGGAAGAGCTCGTATGCCGTCTTCTGCTTG17410.279031004335318Illumina Single End Adapter 1 (100% over 31bp)
TACCTGGTTGATCCTGCCAGTAGCATATG15620.25034257827212336No Hit
ACCGCGGCCTCAGATGAATGCGGC15380.24649608539214193No Hit
ATCCGAATTCAGTCTTCCTGAGGCTCTGGCTCACCTCCAGGCAGG13910.2229363165022558No Hit
CCGATCGCACGCCCCCCGTGGCGGCG13710.21973090576893795No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTG13590.21780765932894727No Hit
ATCAGATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGG12870.20626818068900302No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAAT11710.18767679843575955No Hit
CAAAACCAACCCGGTCAGCCCCTCTCC10720.17181001530583626No Hit
TCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA10310.16523892330253467Illumina Single End Adapter 1 (100% over 31bp)
CGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGGGGAATCA10050.16107188934922148No Hit
ATCGGAAGAGCTCGTATGCCGTCTT9740.15610350271257883Illumina Single End Adapter 1 (100% over 25bp)
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA9550.1530583625159269Illumina Single End Adapter 1 (100% over 32bp)
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAAT9430.15113511607593616No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG8820.14135861333931676Illumina Single End Adapter 1 (100% over 32bp)
CACGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAA8600.13783266153266713No Hit
ATCAAAACCAACCCGGTCAGCCCCTCTCC8510.13639022670267412No Hit
CCCTTCCACCTTCTGCCGTGTTAGGATTTGGTGTTCCTCCCTTCCAGA8440.13526833294601287No Hit
TCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA8360.13398616865268576Illumina Single End Adapter 1 (100% over 31bp)
CGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTC8290.13286427489602448No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGG8210.13158211060269734No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCGC8190.13126156952936557No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTT7550.12100425518274849No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCC7410.118760467669426No Hit
AGTTATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGG7320.11731803283943296No Hit
TCTGGAAGAGGTCTTGCGAAAGCCGCCTCGAGCGCGCTCCGCGGCTGC7080.11347153995945156No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG6970.11170856405612675No Hit
TCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGG6920.11090721137279728No Hit
AAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCGAATG6900.11058667029946549No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCT6790.10882369439614069Illumina Single End Adapter 1 (100% over 27bp)
TTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAATTC6600.10577855419948874No Hit
TCGGAAGAGCTCGTATGCCGTCTTCTGCTTGGA6540.10481693097949339Illumina Single End Adapter 1 (100% over 31bp)
TCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA6450.10337449614950035Illumina Single End Adapter 1 (100% over 31bp)
CTTTGGTGACTCTAGATAACCTCGGG6430.10305395507616856No Hit
ATGGTAGTCGCCGTGCCTACCATGG6420.10289368453950268No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA6280.10064989702618019No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGCCA32350.094.5538544
TTAAGCC32500.064.30578643
ATTAGTC557.676135E-963.8203244
CTATACG250.004047841860.17344344
AGGTCCC3400.059.96953243
ATTAAGC32350.056.12385642
GTAACCG202.6353958E-454.89977640
GTACCGT150.006463478353.47071538
CCCCCTT5050.052.62694544
GATTAAG33100.052.240641
CGTGCGC1750.051.57724444
AGATTAA33500.051.54023740
AAGATTA33650.050.70688639
TATTCGC203.942447E-450.59961333
ACTACAC705.1468305E-850.1445444
AAAGATT34300.049.26165438
CGCGAAA1250.049.19305838
CAAAGAT33850.049.0645337
GTCTCAA33900.047.6890133
TCTCAAA34350.047.4106334
Produced by FastQC (version 0.11.5)