##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR841147.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 623945 Sequences flagged as poor quality 0 Sequence length 24-50 %GC 52 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.90398833230493 32.0 27.0 37.0 20.0 39.0 2 33.4974332673553 35.0 32.0 37.0 26.0 39.0 3 33.51849762398929 35.0 32.0 37.0 26.0 39.0 4 33.983254934329146 35.0 32.0 37.0 27.0 39.0 5 33.561539879316285 34.0 31.0 38.0 26.0 40.0 6 33.280055133064614 34.0 31.0 37.0 26.0 40.0 7 33.15562910192405 34.0 30.0 37.0 26.0 40.0 8 32.49206740978772 33.0 30.0 37.0 24.0 40.0 9 32.86553462244268 34.0 30.0 37.0 26.0 39.0 10 32.20517673833431 33.0 29.0 37.0 24.0 39.0 11 32.43346288535047 33.0 29.0 37.0 24.0 39.0 12 32.29869780188959 33.0 29.0 36.0 24.0 39.0 13 32.348208576076416 33.0 29.0 36.0 25.0 39.0 14 32.52676437827052 34.0 29.0 37.0 25.0 39.0 15 32.12631401806249 33.0 29.0 36.0 24.0 39.0 16 31.889187348243837 33.0 29.0 35.0 24.0 39.0 17 31.833946902371203 33.0 29.0 35.0 24.0 39.0 18 31.820198895736002 33.0 29.0 36.0 23.0 39.0 19 31.29157377653479 32.0 28.0 35.0 22.0 39.0 20 31.234985455448797 32.0 28.0 35.0 23.0 38.0 21 31.073698803580445 32.0 28.0 35.0 22.0 38.0 22 30.604910689243443 32.0 27.0 35.0 21.0 38.0 23 30.84569152729808 32.0 28.0 35.0 22.0 38.0 24 30.680374071432578 32.0 27.0 35.0 22.0 37.0 25 30.455712002864455 32.0 27.0 35.0 21.0 37.0 26 30.257454473367602 31.0 26.0 34.0 22.0 37.0 27 30.1208543377853 31.0 26.0 34.0 21.0 36.0 28 30.426249634074942 31.0 27.0 34.0 22.0 36.0 29 30.686172529044832 32.0 28.0 34.0 22.0 36.0 30 31.29133904517261 33.0 29.0 35.0 23.0 36.0 31 31.674732442002636 33.0 30.0 35.0 24.0 36.0 32 32.11260992696378 33.0 30.0 35.0 25.0 37.0 33 32.187836477526055 33.0 31.0 35.0 26.0 36.0 34 32.22457938897786 33.0 31.0 35.0 26.0 36.0 35 32.236736200310276 33.0 31.0 35.0 26.0 36.0 36 32.21534191450186 34.0 31.0 35.0 25.0 36.0 37 32.2195697192821 33.0 31.0 35.0 26.0 36.0 38 32.123232714554746 33.0 31.0 35.0 25.0 36.0 39 32.444662642184156 34.0 31.0 35.0 27.0 36.0 40 32.34070054180957 33.0 31.0 35.0 27.0 36.0 41 32.14001259821636 33.0 31.0 35.0 26.0 35.0 42 32.19208426784499 33.0 31.0 35.0 27.0 35.0 43 32.15524701469774 33.0 31.0 35.0 27.0 35.0 44 32.196044455066925 34.0 31.0 35.0 27.0 35.0 45 32.09247032658208 33.0 31.0 35.0 26.0 35.0 46 32.100073163248446 33.0 31.0 35.0 26.0 35.0 47 32.02145729497733 33.0 31.0 35.0 26.0 35.0 48 31.933816573374962 33.0 31.0 35.0 26.0 35.0 49 32.16655968442183 33.0 31.0 35.0 26.0 35.0 50 31.763753410566874 33.0 30.0 34.0 25.0 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 10.0 14 50.0 15 48.0 16 11.0 17 0.0 18 0.0 19 3.0 20 61.0 21 289.0 22 997.0 23 2788.0 24 6688.0 25 13163.0 26 23164.0 27 35950.0 28 50077.0 29 64252.0 30 73023.0 31 72129.0 32 64477.0 33 59500.0 34 59690.0 35 31738.0 36 21710.0 37 16317.0 38 12775.0 39 10098.0 40 4937.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 42.58356105105418 25.63110530575612 31.785333643189706 2 24.10276546811017 25.516031060430006 27.302566732644706 23.07863673881512 3 23.952271434180897 15.319940058819286 23.813637419964902 36.91415108703492 4 21.947287020490588 22.975101972128954 23.44341248026669 31.634198527113767 5 25.60514147881624 23.775653302775083 28.377020410452847 22.242184807955827 6 32.41487631121333 27.54713957159686 17.98331583713308 22.054668280056735 7 37.56116324355512 23.13585332040484 19.672086481981584 19.63089695405845 8 26.93282260455649 22.194744729102727 27.54265199657021 23.32978066977057 9 23.119505725664922 25.890262763544865 27.97586325717812 23.014368253612098 10 30.977570138393606 20.539150085344062 21.25700181907059 27.22627795719174 11 27.963041614244844 25.68271241856253 23.685581261168853 22.66866470602377 12 25.349029161224145 18.616224186426688 27.247593938568304 28.787152713780863 13 23.899221886544485 17.385346464832637 26.568207133641586 32.14722451498129 14 20.46430374472109 23.65336688329901 23.22143778698443 32.66089158499547 15 22.702642059796936 22.704725576773594 30.695654264398303 23.896978099031166 16 28.56069044547196 26.19044947872008 21.550136630632508 23.698723445175457 17 24.47459311317504 22.93695758440247 22.008029553886963 30.580419748535526 18 26.64593834392455 22.230645329315884 18.797009351785814 32.32640697497375 19 22.33033360312207 26.240934697769834 25.90869387526144 25.520037823846653 20 37.16128825457372 16.566043481396598 19.277340150173494 26.99532811385619 21 27.67439437770957 18.027710775789533 26.732644704260792 27.5652501422401 22 24.56306244941461 24.896104624606334 24.140108503153325 26.40072442282573 23 32.46664369455641 20.537867921050733 21.332809782913557 25.6626786014793 24 27.820240566075537 20.51959707987082 22.08335670611993 29.576805647933714 25 25.474756985425756 24.88296842468206 25.698045274311404 23.94422931558078 26 27.96675051952313 18.74965820846549 28.35622197309417 24.927369298917203 27 24.047901684082397 27.228689907748983 23.592413378261124 25.1309950299075 28 24.552107728337237 18.832150175644028 28.726031908665107 27.88971018735363 29 36.88104766877861 20.31262665583357 21.22765908915251 21.578666586235304 30 25.686830399097797 24.747039659169225 20.69027629847754 28.875853643255432 31 25.604784286326755 19.857447957190722 30.29895834987376 24.23880940660876 32 24.41395785411287 19.92321729987552 26.590944797831067 29.071880048180542 33 30.69673517461171 21.673329991482355 20.44377335729685 27.186161476609083 34 21.883108986844245 22.773281229711433 29.291798832202844 26.051810951241478 35 24.09065507865281 23.74952040969523 22.54929354262932 29.610530969022637 36 24.79926772528731 21.330875544935136 30.086746713516497 23.783110016261055 37 24.59626561674422 20.682492202280514 21.9222443796765 32.79899780129877 38 25.055044390637608 29.94048964218456 19.332795264998655 25.671670702179178 39 23.313427913012156 32.18016430651454 18.450632098358234 26.05577568211507 40 24.17775908965481 32.40213920607597 19.77780113793149 23.642300566337727 41 32.33696913436992 22.15031661306899 19.964194542983122 25.54851970957796 42 28.376795099253506 28.59527555540678 19.155089880494092 23.872839464845626 43 23.080990092298652 22.22375362924636 29.927358586486214 24.767897691968773 44 26.09620900132372 20.659288130607443 26.332456978967496 26.91204588910134 45 26.49855994937722 27.053177184386968 24.31847684984855 22.129786016387264 46 26.51860942625853 30.090156002926534 20.39649760449364 22.994736966321305 47 36.407465864483015 19.35898756248099 18.514251772939236 25.71929480009676 48 25.0817306351022 21.47949569218049 19.9148834720239 33.523890200693415 49 28.340471966761587 21.298900611549413 19.658279258671396 30.7023481630176 50 24.2958759381783 33.767880959230105 19.85146055074662 22.084782551844974 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 860.0 1 895.5 2 1276.0 3 1556.0 4 1405.0 5 988.0 6 602.0 7 534.0 8 524.0 9 522.5 10 506.5 11 448.5 12 378.0 13 414.0 14 480.0 15 512.5 16 555.5 17 665.5 18 798.0 19 1034.5 20 1269.5 21 1543.5 22 1878.5 23 2310.0 24 2686.0 25 3098.0 26 3678.5 27 4391.5 28 5096.5 29 5779.5 30 6625.5 31 7828.5 32 8977.5 33 10053.0 34 11256.5 35 12537.0 36 14184.5 37 16938.0 38 21000.5 39 25545.5 40 27356.0 41 27354.0 42 28233.5 43 43327.5 44 58173.5 45 48966.0 46 40067.5 47 36724.5 48 34954.0 49 38341.0 50 42549.0 51 45627.5 52 48443.0 53 51689.5 54 52239.0 55 50218.5 56 45012.0 57 41886.5 58 43314.5 59 43859.5 60 41239.0 61 38599.0 62 36553.0 63 34506.0 64 36828.5 65 39115.0 66 33554.5 67 26097.5 68 23311.5 69 21586.0 70 18526.0 71 20439.5 72 23704.0 73 17541.5 74 11120.5 75 11460.5 76 11947.0 77 8767.5 78 5649.5 79 6072.5 80 6391.0 81 4556.5 82 2912.0 83 3033.5 84 3196.0 85 2614.5 86 2051.5 87 1139.5 88 311.0 89 241.5 90 194.5 91 140.0 92 110.5 93 98.5 94 56.0 95 11.5 96 7.5 97 7.0 98 5.5 99 4.0 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 24 20689.0 25 18104.0 26 21581.0 27 17011.0 28 16087.0 29 19721.0 30 6936.0 31 7350.0 32 5720.0 33 5559.0 34 5611.0 35 5437.0 36 4771.0 37 4743.0 38 3779.0 39 4228.0 40 4173.0 41 4350.0 42 5510.0 43 7449.0 44 5290.0 45 6136.0 46 6183.0 47 23244.0 48 51547.0 49 113302.0 50 229434.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 27.815373575765044 #Duplication Level Percentage of deduplicated Percentage of total 1 62.532996391232544 17.39378655434101 2 13.121737744357997 7.299720746450687 3 6.6848547904819835 5.57825199890997 4 4.190307950991845 4.66219924217347 5 3.0021712074699054 4.175325683709053 6 2.0663273207917143 3.4485399814578743 7 1.5862466157880222 3.088542954100582 8 1.2184974295725877 2.7114368963736792 9 0.9338496797401687 2.337783993502321 >10 4.31590411224483 19.908403770192713 >50 0.17911411086423457 3.4943263464941277 >100 0.13287223256921765 7.331051738122691 >500 0.019901567873803525 3.9635579432975505 >1k 0.012877485094814047 6.385913147059504 >5k 0.0011706804631649134 1.9404240703343827 >10k+ 0.0011706804631649134 6.280734933480378 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA 28460 4.561299473511287 No Hit CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGC 10116 1.6212967489121637 No Hit CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGG 6797 1.089358837718068 No Hit CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCG 5121 0.8207454182660331 No Hit ATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGC 3021 0.4841772912676598 No Hit CAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGCGC 2607 0.4178252890879805 No Hit ATCCGAATTCAGTCTTCCTGAGGCTCTGGCTCACCTCCAGGCAGGTCCC 2495 0.39987498898140056 No Hit TCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGC 2476 0.3968298487847487 No Hit TCGGAAGAGCTCGTATGCCGTCTTCT 2360 0.37823846653150517 Illumina Single End Adapter 1 (100% over 26bp) AACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTT 2355 0.37743711384817574 No Hit CTCCTACTTGGATAACTGTGGTAATT 1941 0.3110851116684964 No Hit ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA 1940 0.3109248411318305 No Hit TCGGAAGAGCTCGTATGCCGTCTT 1862 0.2984237392718909 Illumina Single End Adapter 1 (100% over 24bp) CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGC 1850 0.2965004928319003 No Hit ATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGG 1787 0.28640344902194903 No Hit TCGGAAGAGCTCGTATGCCGTCTTCTGCTTG 1741 0.279031004335318 Illumina Single End Adapter 1 (100% over 31bp) TACCTGGTTGATCCTGCCAGTAGCATATG 1562 0.25034257827212336 No Hit ACCGCGGCCTCAGATGAATGCGGC 1538 0.24649608539214193 No Hit ATCCGAATTCAGTCTTCCTGAGGCTCTGGCTCACCTCCAGGCAGG 1391 0.2229363165022558 No Hit CCGATCGCACGCCCCCCGTGGCGGCG 1371 0.21973090576893795 No Hit TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTG 1359 0.21780765932894727 No Hit ATCAGATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGG 1287 0.20626818068900302 No Hit ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAAT 1171 0.18767679843575955 No Hit CAAAACCAACCCGGTCAGCCCCTCTCC 1072 0.17181001530583626 No Hit TCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA 1031 0.16523892330253467 Illumina Single End Adapter 1 (100% over 31bp) CGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGGGGAATCA 1005 0.16107188934922148 No Hit ATCGGAAGAGCTCGTATGCCGTCTT 974 0.15610350271257883 Illumina Single End Adapter 1 (100% over 25bp) ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA 955 0.1530583625159269 Illumina Single End Adapter 1 (100% over 32bp) ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAAT 943 0.15113511607593616 No Hit ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG 882 0.14135861333931676 Illumina Single End Adapter 1 (100% over 32bp) CACGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAA 860 0.13783266153266713 No Hit ATCAAAACCAACCCGGTCAGCCCCTCTCC 851 0.13639022670267412 No Hit CCCTTCCACCTTCTGCCGTGTTAGGATTTGGTGTTCCTCCCTTCCAGA 844 0.13526833294601287 No Hit TCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA 836 0.13398616865268576 Illumina Single End Adapter 1 (100% over 31bp) CGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTC 829 0.13286427489602448 No Hit CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGG 821 0.13158211060269734 No Hit AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCGC 819 0.13126156952936557 No Hit ACCGCGGCCTCAGATGAATGCGGCTGTT 755 0.12100425518274849 No Hit CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCC 741 0.118760467669426 No Hit AGTTATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGG 732 0.11731803283943296 No Hit TCTGGAAGAGGTCTTGCGAAAGCCGCCTCGAGCGCGCTCCGCGGCTGC 708 0.11347153995945156 No Hit CGGCAAGTTTGAATTTCGTGGAGGCTCGGG 697 0.11170856405612675 No Hit TCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGG 692 0.11090721137279728 No Hit AAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCGAATG 690 0.11058667029946549 No Hit ATCGGAAGAGCTCGTATGCCGTCTTCT 679 0.10882369439614069 Illumina Single End Adapter 1 (100% over 27bp) TTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAATTC 660 0.10577855419948874 No Hit TCGGAAGAGCTCGTATGCCGTCTTCTGCTTGGA 654 0.10481693097949339 Illumina Single End Adapter 1 (100% over 31bp) TCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA 645 0.10337449614950035 Illumina Single End Adapter 1 (100% over 31bp) CTTTGGTGACTCTAGATAACCTCGGG 643 0.10305395507616856 No Hit ATGGTAGTCGCCGTGCCTACCATGG 642 0.10289368453950268 No Hit AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA 628 0.10064989702618019 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.15930891344589668 0.0 0.0 0.0 0.0 2 0.24056607553550394 0.0 0.0 0.0 0.0 3 0.3051551018118584 0.0 0.0 0.0 0.0 4 0.431929096314579 0.0 0.0 0.0 0.0 5 0.5617482310139515 0.0 0.0 0.0 0.0 6 0.7002219746932823 0.0 0.0 0.0 0.0 7 0.7811585957095577 0.0 0.0 0.0 0.0 8 0.8515173613058843 0.0 0.0 0.0 0.0 9 0.9092147545056055 0.0 0.0 0.0 0.0 10 0.9633861958986769 0.0 0.0 0.0 0.0 11 1.0087427577751245 0.0 0.0 0.0 0.0 12 1.0432009231582913 0.0 0.0 0.0 0.0 13 1.0832685573247642 0.0 0.0 0.0 0.0 14 1.1289456602745433 0.0 0.0 0.0 0.0 15 1.1664489658543622 0.0 0.0 0.0 0.0 16 1.1989838847975383 0.0 0.0 0.0 0.0 17 1.2313585332040484 0.0 0.0 0.0 0.0 18 1.2552388431672663 0.0 0.0 0.0 0.0 19 1.279439694203816 0.0 0.0 0.0 0.0 20 1.300274863970382 0.0 0.0 0.0 0.0 21 1.3163019176369712 0.0 0.0 0.0 0.0 22 1.3312070775468992 0.0 0.0 0.0 0.0 23 1.3494779187268109 0.0 0.0 0.0 0.0 24 1.3645433491734047 0.0 0.0 0.0 0.0 25 1.3797690501566644 0.0 0.0 0.0 0.0 26 1.3945139395299264 0.0 0.0 0.0 0.0 27 1.4167915441264856 0.0 0.0 0.0 0.0 28 1.429452916523091 0.0 0.0 0.0 0.0 29 1.4433964532130237 0.0 0.0 0.0 0.0 30 1.4542948497063042 0.0 0.0 0.0 0.0 31 1.4655137872729167 0.0 0.0 0.0 0.0 32 1.473847855179543 0.0 0.0 0.0 0.0 33 1.4818613820128377 0.0 0.0 0.0 0.0 34 1.4948432954827748 0.0 0.0 0.0 0.0 35 1.5071841268060486 0.0 0.0 0.0 0.0 36 1.515678465249341 0.0 0.0 0.0 0.0 37 1.5236919920826355 0.0 0.0 0.0 0.0 38 1.5315452483792642 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAAGCCA 3235 0.0 94.55385 44 TTAAGCC 3250 0.0 64.305786 43 ATTAGTC 55 7.676135E-9 63.82032 44 CTATACG 25 0.0040478418 60.173443 44 AGGTCCC 340 0.0 59.969532 43 ATTAAGC 3235 0.0 56.123856 42 GTAACCG 20 2.6353958E-4 54.899776 40 GTACCGT 15 0.0064634783 53.470715 38 CCCCCTT 505 0.0 52.626945 44 GATTAAG 3310 0.0 52.2406 41 CGTGCGC 175 0.0 51.577244 44 AGATTAA 3350 0.0 51.540237 40 AAGATTA 3365 0.0 50.706886 39 TATTCGC 20 3.942447E-4 50.599613 33 ACTACAC 70 5.1468305E-8 50.14454 44 AAAGATT 3430 0.0 49.261654 38 CGCGAAA 125 0.0 49.193058 38 CAAAGAT 3385 0.0 49.06453 37 GTCTCAA 3390 0.0 47.68901 33 TCTCAAA 3435 0.0 47.41063 34 >>END_MODULE