FastQCFastQC Report
Tue 24 May 2016
ERR841146.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841146.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2340958
Sequences flagged as poor quality0
Sequence length24-50
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA254641.0877597974846196No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCC146670.6265383659168596No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGC137610.5878362619064502No Hit
ATCCGAATTCAGTCTTCCTGAGGCTCTGGCTCACCTCCAGGCAGGTCCC132670.5667337901833351No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA124790.53307235755618No Hit
CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGG115520.4934731849097677No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC106630.4554972793189797No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA99290.42414259461297465No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCCG94560.4039371915258625No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGC93000.3972732530869841No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAAG92240.39402671897573555No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGAC89120.3806988420979787No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGA87570.37407762121319565No Hit
CTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAATCCT86130.36792629342346167No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAAT79690.34041618858604045No Hit
AGGCCTGGTTTGCGCTGGAGAGTGGTCTTGGTTGTGAGGGTCATAAGAT65150.2783048649313657No Hit
ATCCGAATTCAGTCTTCCTGAGGCTCTGGCTCACCTCCAGGCAGGTCCCA65070.2779631244986027No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA64520.2756136590233571Illumina Single End Adapter 1 (100% over 32bp)
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCG64040.2735632164267791No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAAT61290.2618158890505511No Hit
CTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAATCCTG59190.2528452026905224No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA58850.2513928058512797Illumina Single End Adapter 1 (100% over 32bp)
CGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGT58150.24840257706460347No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG55830.23849210451447656No Hit
ATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGC51630.2205507317944192No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCC46890.20030261115321163No Hit
CCCTTCCACCTTCTGCCGTGTTAGGATTTGGTGTTCCTCCCTTCCAGA46250.19756868769110766No Hit
TCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA44930.1919299705505182Illumina Single End Adapter 1 (100% over 31bp)
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATC41580.17761958992856772No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA39580.16907607910949277No Hit
CTCTGCAGGTACCATGGAGCTGAGCTATAGGCTCTTCATCTGCCTCCT38290.16356551463118946No Hit
CAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGCGC38010.16236942311651897No Hit
CTCTGCAGGTACCATGGAGCTGAGCTATAGGCTCTTCATCTGCCTCCTG35840.15309971387782267No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTAG34890.14904154623876206No Hit
AGCGAGCGGCCTTGAGAGGCTCTGGCTCTTGCTTCTTAGGCGGCCCGAG34150.14588044723570437No Hit
TCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA32450.13861846303949069Illumina Single End Adapter 1 (100% over 31bp)
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGT31110.13289431079071046No Hit
CTGTGCGTCTGCTCCTCCTTCTCGCGCTTCTCCTGCCGCCCTAATCCT29950.127939074515647No Hit
TCGCTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAAT29760.12712744098783488No Hit
ATTAGGCCTGGTTTGCGCTGGAGAGTGGTCTTGGTTGTGAGGGTCATA27870.1190538232638091No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA27620.11798588441142473Illumina Single End Adapter 1 (100% over 32bp)
AGGCCTGGTTTGCGCTGGAGAGTGGTCTTGGTTGTGAGGGTCATAAGATG27550.11768686153275709No Hit
CCCTTCCACCTTCTGCCGTGTTAGGATTTGGTGTTCCTCCCTTCCAGAA27090.11572185404436987No Hit
CGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTC25830.11033944222835267No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCG25160.10747736610396257No Hit
AGCTGTTTTTCGTCTTCCCTAGGCTATTTCTGCCGGGCGCTCCGCGAAG24980.10670845013024581No Hit
ACCAGCGAGCGGCCTTGAGAGGCTCTGGCTCTTGCTTCTTAGGCGGCCC24410.10427354954680947No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCGC23530.1005144047864165No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTAGTC12600.0123.7361844
TAAGCCA18700.096.77228544
CTATACG1300.085.6635244
GGTCCCA14700.076.2303444
AATGAAG17800.071.9477244
CCAGAAT13450.068.8253844
TATTAGT12750.059.62600343
CGCTACC3300.056.9467744
CAATGAA42050.056.31759643
CGACGGG30700.054.86511244
AGGTCCC26800.053.4702643
TCGTGCG10650.051.1685243
ATAAGAT15850.050.5109143
TTAAGCC17700.050.1727843
AATCGCA702.1582206E-549.71543544
AATCCTG16300.049.52917543
GACCTTA3400.047.4897443
TAAGATG13100.046.75527644
AATCCCG13750.046.2377443
TTTGGAC16200.045.8896543
Produced by FastQC (version 0.11.5)